Use of fresh vegetable discards as sea urchin diet: effect on gonad index and quality     

Salvatrice Vizzini  Luca Miccichè  Antonino Vaccaro  Antonio Mazzola 《Aquaculture International》2015,23(1):127-139

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Completeness of the dog registry and estimation of the dog population size in a densely populated area of Rome     

Antonino Caminiti  Marcello Sala  Valentina Panetta  Sabrina Battisti  Roberta Meoli  Pasquale Rombolà  Valentina Spallucci  Claudia Eleni  Paola Scaramozzino 《Preventive veterinary medicine》2014

In most European countries, registration and identification of dogs is compulsory. In Italy, the national dog registry is composed of regional dog registries. Although dog registries have been established for many years, the issue related to completeness of data has not been addressed so far. The objective of this study was twofold: first to assess the completeness of data of the dog registry through telephone interview of a sample of dog owners drawn from the dog registry, then to estimate the total owned dog population in 4 boroughs of Rome. For the second objective, a capture–recapture method was applied using data from the dog registry and data from a face-to-face questionnaire submitted to people waiting in the sitting room of 5 points of access for booking and payment of primary and specialist care. Different scenarios are proposed to verify the assumptions of the estimation procedure and potential biases are discussed. The completeness of data of the dog registry was 88.9% (95% CI: 85.8–91.9%) and the owned-dog population was estimated at 26,244 dogs (95% CI: 24,110–28,383). The dog registry is an important source of information especially when it is properly updated and completeness of data is known.  相似文献   

Forage chain arrangement for sustainable livestock systems in a Mediterranean area     

S. L. Cosentino  F. Gresta  G. Testa 《Grass and Forage Science》2014,69(4):625-634

Mediterranean forage systems suffer from limited availability of fresh forage because of water deficits and extreme temperatures. Consequently, fresh forage is unavailable for at least 6–7 months a year, and farmers must buy feed to support livestock production. With the aim of overcoming these limitations, a 2‐year trial was conducted on three distinct sites in Sicily (at 10, 600 and 1200 m elevation) with thirty‐four varieties of forage species belonging to nine biennial/perennial and thirteen annual species. Results showed that by integrating grasses and legumes, species from environments with different climatic conditions enable the season of forage production to be extended from mid‐April to mid‐November. Quality traits of forage in different areas varied in relation to species and varieties. In general, the sown‐forage quality was better than in pastures and fallows in the same areas commonly used to feed animals. This also leads to a reduction in the use of supplementary feeds. Among the tested species, Lolium multiflorum and Medicago sativa emerged as the most promising for filling the forage‐deficit periods, and Trifolium spp. and Vicia sativa were found to be superior for increasing forage quality. The results are discussed in the context of adapting Mediterranean forage supplies for ensuring greater sustainability of livestock production in mountain, hill and plain areas. The proposed forage chain arrangement represents part of local potential adaptation to climate limitations and climate change.  相似文献   

Dry matter and qualitative characteristics of alfalfa as affected by harvest times and soil water content     

G. Testa  F. Gresta  S.L. Cosentino 《European Journal of Agronomy》2011,34(3):144-152

The best trade-off between quantity and quality in forage crops is one the most important targets of the agronomic research, since quantitative and qualitative traits are generally inversely correlated. With the aim of exploring the effect of harvest time (early and late), soil water content (irrigated and rainfed) and variety (Equipe, Europe and Midi) on duration, yield and quality of alfalfa, a four year trial was carried out in a Mediterranean environment. Early cut resulted in a reduction of forage yield (12%) with a faster reduction of longevity, but with a higher quality characteristics in terms of protein (20.2%) and fibre (23.1%) compared to the late cut (18.0 and 25.9%, respectively for protein and fibre). Dry treatment led to a limited reduction of yield and quality traits, suggesting alfalfa is a potential forage resource for the improvement of hilly areas of Mediterranean environment. The tested varieties show all a good regrowth capability allowing three, sometimes four, cut per year. The obtained results pointed out that the combination of early and late harvest times would allow a better distribution of the forage production between the months of June and September as well as a fair useful forage regrowth during the months of October and November.  相似文献   

Mitotic Golgi partitioning is driven by the membrane-fissioning protein CtBP3/BARS     

Hidalgo Carcedo C  Bonazzi M  Spanò S  Turacchio G  Colanzi A  Luini A  Corda D 《Science (New York, N.Y.)》2004,305(5680):93-96

Organelle inheritance is an essential feature of all eukaryotic cells. As with other organelles, the Golgi complex partitions between daughter cells through the fission of its membranes into numerous tubulovesicular fragments. We found that the protein CtBP3/BARS (BARS) was responsible for driving the fission of Golgi membranes during mitosis in vivo. Moreover, by in vitro analysis, we identified two stages of this Golgi fragmentation process: disassembly of the Golgi stacks into a tubular network, and BARS-dependent fission of these tubules. Finally, this BARS-induced fission of Golgi membranes controlled the G2-to-prophase transition of the cell cycle, and hence cell division.  相似文献   

Space astronomy     

Testa JP  Arnold JR 《Science (New York, N.Y.)》1983,219(4583):338

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Genetics. Ethical aspects of ES cell-derived gametes     

Testa G  Harris J 《Science (New York, N.Y.)》2004,305(5691):1719

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Identification,partial characterization,and use of grey mullet ( Mugil cephalus ) vitellogenins for the development of ELISA and biosensor immunoassays     

Paolo Cocci  Francesco Alessandro Palermo  Stefania Pucciarelli  Antonino Miano  Massimiliano Cuccioloni  Mauro Angeletti  Alessandra Roncarati  Gilberto Mosconi 《International Aquatic Research》2019,11(4):389-399

Vitellogenin (Vtg) has proven to be a sensitive and simple biomarker in determining sex, sexual maturity, and xenoestrogenic effects in fish. Thus, our investigation has been focused on identification, partial characterization, and quantification of grey mullet (Mugil cephalus) Vtg through the use of a variety of biochemical and immunological analytical techniques. Mullet is considered both a promising aquaculture candidate and an important species for improving sediment quality in polyculture systems. In the first part of this work, grey mullet Vtg was purified from plasma of 17β-estradiol (E2)-induced male fish by a one-step chromatographic protocol, and partially characterized. Specific polyclonal antibodies were then raised against the mullet Vtg, and both an indirect ELISA and an optical immunosensor were set up and validated to quantify plasma Vtg. The indirect ELISA and the optical immunosensor assay developed showed linear measuring in the range 56.8–1047.1 ng mL−1 and 70–739 ng mL−1 Vtg concentrations in standard solutions, respectively. The results obtained suggest that the indirect ELISA allows Vtg detection over a wide dynamic range, thus resulting more suitable for rapid and sensitive sample screening. Therefore, we suggest that the direct immunosensor is a promising tool which needs more investigation to improve the sensitivity.  相似文献   

Effects of n-3 Polyunsaturated Fatty Acids (ω-3) Supplementation on Some Cardiovascular Risk Factors with a Ketogenic Mediterranean Diet     

Antonio Paoli  Tatiana Moro  Gerardo Bosco  Antonino Bianco  Keith A. Grimaldi  Enrico Camporesi  Devanand Mangar 《Marine drugs》2015,13(2):996-1009

Background: the ketogenic diet (KD) has become a widely used nutritional approach for weight loss. Some of the KD’s positive effects on metabolism and cardiovascular risk factors are similar to those seen after n-3 polyunsaturated fatty acids (ω-3) supplementation. We hypothesized that a ketogenic Mediterranean diet with phytoextracts combined with ω-3 supplementation may have increased positive effects on cardiovascular risk factors and inflammation. Methods: We analyzed 34 male overweight subjects; aged between 25 and 65 years who were overall healthy apart from overweight. The subjects followed a ketogenic diet protocol for four weeks; with (KDO3) or without (KD) ω-3 supplementation. Results: All subjects experienced a significant loss of body weight and body fat and there was no significant differences between treatment (body weight: KD—4.7 kg, KDO3—4.03 kg, body fat KD—5.41 kg, KDO3—5.86 kg). There were also significant decreases in total cholesterol, LDL-c, and glucose levels. Triglycerides and insulin levels decreased more in KDO3 vs. KD subjects, with a significant difference. All the investigated inflammatory cytokines (IL-1β, IL-6, TNF-α) decreased significantly in KDO3 subjects whilst only TNF-α showed a significant decrease in KD subjects over the 12 month study period. No significant changes were observed in anti-inflammatory cytokines (IL-10 and IL-1Ra), creatinine, urea and uric acid. Adiponectin increased significantly only in the KDO3 group. Conclusions: ω-3 supplementation improved the positive effects of a ketogenic Mediterranean diet with phytoextracts on some cardiovascular/metabolic risk factors and inflammatory state.  相似文献   

Hymenoptera wasps associated with the Asian gall wasp of chestnut (Dryocosmus kuriphilus) in Calabria,Italy   总被引：1，自引：0，他引：1  

Vincenzo Palmeri  Pasquale Cascone  Orlando Campolo  Saverio B. Grande  Francesca Laudani  Antonino Malacrinò  Emilio Guerrieri 《Phytoparasitica》2014,42(5):699-702

The parasitoid complex of the Asian chestnut gall wasp Drycosmus kuriphilus Yasumatsu was studied in Calabria (Italy). A total of 14 different species of parasitoids were collected, of which three are recorded on the Asian gall wasp for the first time. The composition of the parasitoid complex collected in Calabria was compared with that reported from Italy and from Europe. The altitude of the sites of collection seemed to have an effect on the distribution and abundance of the single species of parasitoids.  相似文献