Artificial suckling in Martina Franca donkey foals: effect on in vivo performances and carcass composition     

Pasquale De Palo  Aristide Maggiolino  Paola Milella  Nicola Centoducati  Alessandro Papaleo  Alessandra Tateo 《Tropical animal health and production》2016,48(1):167-173

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Rapid communication: nucleotide sequence of chamois, alpine ibex, and red deer tRNA(Lys) and ATPase8 mitochondrial genes     

Saulle E  Di Pasquale S  Tartaglia M 《Journal of animal science》1999,77(12):3398-3399

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Prevalence and diversity of Aeromonas and Vibrio spp. in coastal waters of Southern Italy     

Dumontet S  Krovacek K  Svenson SB  Pasquale V  Baloda SB  Figliuolo G 《Comparative immunology, microbiology and infectious diseases》2000,23(1):53-72

A survey was undertaken to examine sea water and sediment for the presence of Vibrio and Aeromonas spp. along approximately 900 km of coast in Southern Italy during early and late summer. A quantitative analysis was also done to evaluate the water fecal contamination at the stations examined. The results indicate that all the investigated areas were submitted to a wide spatial fluctuation of fecal contamination and that Vibrio and Aeromonas spp. were present in both high and low fecal-contaminated stations. Sixty two percent of the investigated samples were positive for Aeromonas spp., while 42% of samples were positive for Vibrio spp. It was interesting to note that 38% of the positive stations for both Aeromonas and Vibrio spp. showed a fecal coliform contamination of water at < 10(2) cells 100 ml(-1). Thus, these findings support the hypothesis that the bacterial indicators (such as fecal coliforms) do not always satisfactorily reflect the hygienic quality of water. The presence of Vibrionaceae on copepods was also investigated. Copepods were sampled at a station located inside the harbour of the city of Naples and were found contaminated by V. cholerae non-O1, V. alginolyticus, V. fluvialis and A. caviae. Furthermore, the antibiotic resistance patterns of isolated bacteria showed the presence of a number of resistant strains among the isolates. In order to discriminate the isolates on the basis of their biochemical profiles and/or antibiotic resistance patterns, cluster analysis was carried out which showed that no unique assay could fully discern these isolates. However, the best discrimination resulted from complete pattern profile based on both biochemical profiles and antibiotic resistance patterns.  相似文献   

Use of Degenerate Primers in a RT-PCR Assay for the Identification and Analysis of Some Filamentous Viruses, with Special Reference to Clostero- and Vitiviruses of the Grapevine     

Pasquale Saldarelli  Adib Rowhani  Geoffrey Routh  Angelantonio Minafra  Michele Digiaro 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(9):945-950

RT-PCR with degenerate primers was used for the screening of the genome of some members of the Closterovirus, Vitivirus and Trichovirus genera. Two sets of primers, targeted to conserved sequences of the heat shock protein 70 homologue of closteroviruses or to the RNA dependent RNA polymerase genes of tricho- and vitiviruses, amplified the expected fragments from total RNA extracts or double-stranded RNAs of infected plants. Amplified cDNAs were cloned, sequenced and phylogenetically analyzed. Results support the allocation of grapevine viruses A, B, D and heracleum latent virus (HLV) in the genus Vitivirus, whereas, the detection of a HSP70 homologue in grapevine leafroll-associated viruses agrees with their assignment in the genus Closterovirus. The use of degenerate primers for the identification of grapevine viruses belonging to Vitivirus and Closterovirus genera is envisaged.  相似文献   

A systematic and quantitative approach to improve water use efficiency in agriculture   总被引：6，自引：2，他引：4  

Theodore C. Hsiao  Pasquale Steduto  Elias Fereres 《Irrigation Science》2007,25(3):209-231

As the competition for the finite water resources on earth increases due to growth in population and affluence, agriculture is faced with intensifying pressure to improve the efficiency of water used for food production. The causes for the relatively low water use efficiency in agriculture are numerous and complex, including environmental, biological, engineering, management, social, and economic facets. The complexity of the problem, with its myriads of local variations, requires a comprehensive conceptual framework of the underlying physical and biological processes as the basis to analyze the existing situation and quantify the efficiencies, and to plan and execute improvements. This paper proposes such a framework, based on the simple fact that the overall efficiency of any process consisting of a chain of sequential step is the product of the efficiency (i.e., output/input ratio) of its individual component steps. In most cases of water use, a number of process chains, both branching and merging, are involved. Means to integrate the diverging and converging chains are developed and presented as equations. Upscaling from fields to regions and beyond are discussed. This chain of efficiencies approach is general and can be applied to any process composed of chains of sequential steps. Here the framework is used to analyze the systems of irrigated and dryland crop production, and animal production on rangeland. Range of plausible efficiencies of each step is presented as tables, with values separately for the poor and for the good situation of circumstances, management and technology. Causes of the differences in efficiency of each step, going from water delivery to soil water extraction, transpiration, photosynthesis, and conversion to crop biomass and yield, and to animal product are briefly discussed. Sample calculations are made to demonstrate how modest differences in the efficiencies of the component steps are manifested as large to huge differences in the overall efficiency. Based on an equation quantifying the impact of changes in efficiency of component steps on the overall efficiency, it is concluded that generally, it is more effective to made modest improvements in several or more steps than to concentrate efforts to improve one or two steps. Hence, improvement efforts should be systematic and not overly concentrated on one or two components. The potential use of the same equation as the point of departure to optimize the allocation of economic resource among the component steps to maximize the improvement in the overall water use efficiency is elaborated on. The chain of efficiencies framework provides the means to examine the current levels of efficiency along the pathways of agricultural water use, to analyze where inefficiencies lie by comparing with the range of known efficiency values in the tables presented, to assess the potential improvements that may be achieved in various parts and their impact on the overall efficiency, and to aid in the optimal allocation of resources for improvements.

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Theodore C. HsiaoEmail:

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Completeness of the dog registry and estimation of the dog population size in a densely populated area of Rome     

Antonino Caminiti  Marcello Sala  Valentina Panetta  Sabrina Battisti  Roberta Meoli  Pasquale Rombolà  Valentina Spallucci  Claudia Eleni  Paola Scaramozzino 《Preventive veterinary medicine》2014

In most European countries, registration and identification of dogs is compulsory. In Italy, the national dog registry is composed of regional dog registries. Although dog registries have been established for many years, the issue related to completeness of data has not been addressed so far. The objective of this study was twofold: first to assess the completeness of data of the dog registry through telephone interview of a sample of dog owners drawn from the dog registry, then to estimate the total owned dog population in 4 boroughs of Rome. For the second objective, a capture–recapture method was applied using data from the dog registry and data from a face-to-face questionnaire submitted to people waiting in the sitting room of 5 points of access for booking and payment of primary and specialist care. Different scenarios are proposed to verify the assumptions of the estimation procedure and potential biases are discussed. The completeness of data of the dog registry was 88.9% (95% CI: 85.8–91.9%) and the owned-dog population was estimated at 26,244 dogs (95% CI: 24,110–28,383). The dog registry is an important source of information especially when it is properly updated and completeness of data is known.  相似文献   

Expert knowledge for translating land cover/use maps to General Habitat Categories (GHC)     

Maria Adamo  Cristina Tarantino  Valeria Tomaselli  Vasiliki Kosmidou  Zisis Petrou  Ioannis Manakos  Richard M. Lucas  Caspar A. Mücher  Giuseppe Veronico  Carmela Marangi  Vito De Pasquale  Palma Blonda 《Landscape Ecology》2014,29(6):1045-1067

Monitoring biodiversity at the level of habitats and landscape is becoming widespread in Europe and elsewhere as countries establish international and national habitat conservation policies and monitoring systems. Earth Observation (EO) data offers a potential solution to long-term biodiversity monitoring through direct mapping of habitats or by integrating Land Cover/Use (LC/LU) maps with contextual spatial information and in situ data. Therefore, it appears necessary to develop an automatic/semi-automatic translation framework of LC/LU classes to habitat classes, but also challenging due to discrepancies in domain definitions. In the context of the FP7 BIO_SOS (www.biosos.eu) project, the authors demonstrated the feasibility of the Food and Agricultural Organization Land Cover Classification System (LCCS) taxonomy to habitat class translation. They also developed a framework to automatically translate LCCS classes into the recently proposed General Habitat Categories classification system, able to provide an exhaustive typology of habitat types, ranging from natural ecosystems to urban areas around the globe. However discrepancies in terminology, plant height criteria and basic principles between the two mapping domains inducing a number of one-to-many and many-to-many relations were identified, revealing the need of additional ecological expert knowledge to resolve the ambiguities. This paper illustrates how class phenology, class topological arrangement in the landscape, class spectral signature from multi-temporal Very High spatial Resolution (VHR) satellite imagery and plant height measurements can be used to resolve such ambiguities. Concerning plant height, this paper also compares the mapping results obtained by using accurate values extracted from LIght Detection And Ranging (LIDAR) data and by exploiting EO data texture features (i.e. entropy) as a proxy of plant height information, when LIDAR data are not available. An application for two Natura 2000 coastal sites in Southern Italy is discussed.  相似文献   

Towards a Pan‐European Assessment of Land Susceptibility to Wind Erosion     

Pasquale Borrelli  Panos Panagos  Cristiano Ballabio  Emanuale Lugato  Melanie Weynants  Luca Montanarella 《Land Degradation \u0026amp; Development》2016,27(4):1093-1105

Understanding spatial and temporal patterns in land susceptibility to wind erosion is essential to design effective management strategies to control land degradation. The knowledge about the land surface susceptible to wind erosion in European contexts shows significant gaps. The lack of researches, particularly at the landscape to regional scales, prevents national and European institutions from taking actions aimed at an effective mitigating of land degradation. This study provides a preliminary pan‐European assessment that delineates the spatial patterns of land susceptibility to wind erosion and lays the groundwork for future modelling activities. An Index of Land Susceptibility to Wind Erosion (ILSWE) was created by combining spatiotemporal variations of the most influential wind erosion factors (i.e. climatic erosivity, soil erodibility, vegetation cover and landscape roughness). The sensitivity of each input factor was ranked according to fuzzy logic techniques. State‐of‐the‐art findings within the literature on soil erodibility and land susceptibility were used to evaluate the outcomes of the proposed modelling activity. Results show that the approach is suitable for integrating wind erosion information and environmental factors. Within the 34 European countries under investigation, moderate and high levels of land susceptibility to wind erosion were predicted, ranging from 25·8 to 13·0 M ha, respectively (corresponding to 5·3 and 2·9% of total area). New insights into the geography of wind erosion susceptibility in Europe were obtained and provide a solid basis for further investigations into the spatial variability and susceptibility of land to wind erosion across Europe. © 2014 The Authors. Land Degradation and Development published by John Wiley & Sons, Ltd.  相似文献   

Magma ascent and the pressurization of Mount Etna's volcanic system     

Patanè D  De Gori P  Chiarabba C  Bonaccorso A 《Science (New York, N.Y.)》2003,299(5615):2061-2063

After a period of deflation during the 1991-1993 flank eruption, Mount Etna underwent a rapid inflation. Seismicity and ground deformation show that since 1994, a huge volume of magma intruded beneath the volcano, producing from 1998 onward a series of eruptions at the summit and on the flank of the volcano. The last of these, started on 27 October 2002, is still in progress and can be considered one of the most explosive eruptions of the volcano in recent times. Here we show how geodetic data and seismic deformation, between 1994 and 2001, indicate a radial compression around an axial intrusion, consistent with a repressurization of Mount Etna's plumbing system at a depth of 6 to 15 kilometers, which triggered most of the seismicity and provoked the dilatation of the volcano and the recent explosive eruptive activity.  相似文献   

Durum wheat varieties in N‐deficient environments and organic farming: a comparison of yield,quality and stability performances     

Fabio Stagnari  Andrea Onofri  Pasquale Codianni  Michele Pisante 《Plant Breeding》2013,132(3):266-275

Stability and reliability of yield and quality for 15 durum wheat genotypes (old and modern) were evaluated in a 5‐year experiment (southern Italy) in organic farming. Genotypes were grown at two N levels (0 and 80 kg/ha), with the aim of evaluating ‘genotype × environment’ (GE) interactions and their role on genotype selection in N‐limited environments. Several approaches to stability were used, within the frame of mixed models and additive main effects and multiplicative interaction analysis, and their validity in stressful conditions and organic farming was discussed. Especially for protein and gluten content, results indicate high environmental variability and the presence of crossover ‘N × environment’ interactions, which supports the need for specific breeding programmes in N‐deficient environments. The average response was strongly affected by N availability (on average, yield was 2.95 and 3.42 t/ha, protein content was 11.6% and 12.85%, gluten content was 8.55% and 9.92%, respectively, at 0 and 80 kg N/ha), and few genotypes gave high yield and quality at both fertilization levels. Only ‘Gargano’ and ‘Fortore’ showed a good inter‐year stability at both N levels. The old cultivars gave minimal responsiveness to increased N input, but gave good results in limiting conditions, indicating that they may play a role in organic farming.  相似文献