以球果量、雄花量等数量性状对油松优良无性系的再选择   总被引：1，自引：0，他引：1  

刘梅  安守芹  吕馨  王永红 《内蒙古林业科技》2001,(Z1):6-9

本研究对油松无性系种子园 2 2个无性系的球果量和雄球花量进行观测调查 ,并对数据进行处理。通过无性系内和无性系间的方差分析、聚类分析和秩序排序法对各无性系进行分类和排序。研究结果表明 :无性系间球果量和雄球花量的差异极显著 ,而无性系内差异不显著。并筛选出 1 61 #,6#,2 9#,63 #,78#5个球果数量大 ,占总数的 46% ,雄花量高 ,占总数的 5 7.6%的无性系为优良无性系  相似文献   

High frequency embryogenic callus induction and plant regeneration from mature caryposis of big bluestem and little bluestem     

Yonghong Li  Junping Gao  Shui-zhang Fei 《Scientia Horticulturae》2009

Big bluestem (Andropogon gerardii Vitman) and little bluestem [Schizachyrium scoparium (Michaux) Nash.] are native to the North America and are important forage grasses and ornamental grasses. Both grasses are proposed as ideal biomass producers for cellulosic ethanol production. To apply genetic transformation, which is an important tool for incorporating desirable agronomic traits into plants to both species, however requires an efficient and reproducible regeneration protocol. We used mature caryopses from big and little bluestem as explants and tested the effect of various combinations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) (1, 2, 3, 4 or 5 mg l⁻¹) and kinetin (KT) (0, 0.1 or 0.2 mg l⁻¹) on embryogenic callus induction with LS as the basal medium. The highest percentage of embryogenic calli induction occurred on medium containing 2, 4-D alone at 2 mg l⁻¹ for ‘Bison’ and on medium containing 4 mg l⁻¹ 2, 4-D alone for ‘Bonilla’ big bluestem. For little bluestem, the highest percentage of embryogenic callus induction occurred on medium containing 3 mg l⁻¹ 2, 4-D plus 0.1 mg l⁻¹ kinetin, suggesting that addition of KT is beneficial. Shoot regeneration took place on LS basal medium without any plant growth regulator for both species, although the addition of KT increased both regeneration frequency and the number of shoots produced per callus. Rooting of shoots reaching about 2 cm long occurred readily with or without α-naphthaleneacetic acid (NAA). Rooted plantlets were all successfully established in the soil.  相似文献   

PEG诱导水分胁迫对柑桔幼苗细胞质膜透性及脯氨酸含量的影响   总被引：3，自引：0，他引：3  

张云贵  谢永红  吴学良  毛启才 《果树学报》1995,(Z1)

柑桔叶片相对含水量(RWC)随聚乙二醇(PEG)处理渗透势的降低而依次降低;叶片细胞质膜透性随PEG处理浓度的增加而依次增大;幼苗叶片累积脯氨酸的数量随水分胁迫时间和强度递增。  相似文献   

Effect of cGMP on voltage-gated potassium channel in pulmonary artery smooth muscle cells from rats exposed to chronic hypoxia     

ZHANG Yong-chang  NI Wang  ZHEN Guo-hua  ZHANG Zhen-xiang  XU Yong-jian 《园艺学报》2003,19(8):1012-1015

AIM: To investigate the effect of cGMP on voltage-gated potassium channel in pulmonary artery smooth muscle cells (PASMCs) from rats exposed to chronic hypoxia. METHODS: (1) Wistar rats were randomly divided into control group (group A) and chronic hypoxia group (group B). Then group B received hypoxia 8 hours per day for 4 consecutive weeks. (2) Single PASMC was obtained via acute enzyme separation method. (3) Conventional whole-cell patch clamp technique was used to record resting membrane potential (Em) and ion currents of voltage-gated potassium channel. The changes of ion currents of voltage-gated potassium channel before and after applying cGMP (1 mmol/L), an agonist of protein kinase G (PKG), and cGMP plus H-8 (1 mmol/L), an inhibitor of PKG were compared between two groups. RESULTS: The Em of group B were significantly lower than that of group A. The ion currents of voltage-gated potassium channel in group A and group B were all significantly inhibited by cGMP [control group: from (118.0±5.0) pA/pF to (89.9±16.5) pA/pF, n=6, P＜0.05;chronic hypoxia group: from (81.0±5.0) pA/pF to (56.8±9.1) pA/pF, n=6, P＜0.05]and these effects were reversed by H-8 [control group: from (119.2±10.3) pA/pF to (117.8±9.1) pA/pF, n=6, P＞0.05;chronic hypoxia group: from (96.8±6.2)　pA/pF to (98.0±2.2)　pA/pF, n=6, P＞0.05]. CONCLUSIONS: The currents of voltage-gated potassium channel was inhibited by chronic hypoxic. The inhibitory effect of cGMP on currents of voltage-gated potassium channel in PASMCs from both normal and chronic hypoxic rats may be probably through the phosphorylation of voltage-gated potassium channel.  相似文献   

茎瘤芥新品种涪杂1号的选育   总被引：6，自引：1，他引：6  

陈材林  周光凡  范永红  林合清  刘义华  王彬 《中国蔬菜》2003,1(1):23-24

涪杂1号茎瘤芥母本是胞质雄性不育系96118-3A,父本是优良的茎瘤芥自交系920154。该一代杂种从出苗至现蕾150～155 d(天),属中熟类型。瘤茎近圆球形,加工性能好,品质优,适于四川盆地茎瘤芥产区种植,一般每667 m2产量2 500—3 000 kg,最高可达4 000 kg。  相似文献   

富铁酵母对家兔红细胞免疫功能的影响     

罗永江  胡振英  张新国  罗超应  史彦斌  尚若峰  郑继方  梁纪兰 《中兽医医药杂志》2004,23(6):16-18

为探讨富铁酵母对家兔红细胞免疫功能的影响,将32只家免随机分成富铁酵母高、低剂量组,FeSO4组及空白对照组,分别灌服富铁酵母(Fe元素8 mg/kg、4 mg/kg)、FeSO4(Fe元素4 mg/kg)及空白生理盐水;连续5 d后,检测红细胞C3g受体花环率和复合物(IC)花环率.结果:富铁酵母较FeSO4及空白组能显著提高红细胞C3h受体花环和红细胞复合物(IC)花环的百分率,但高、低剂量间无显著性差异.结论:富铁酵母能显著提高红细胞免疫功能.  相似文献   

亚洲棉GaPP2C24基因的克隆及表达分析     

蔡肖  甄军波  刘琳琳  刘迪  唐丽媛  张素君  李兴河  王海涛  刘存敬  张香云  张建宏  迟吉娜 《华北农学报》2020,35(3):33-39

PP2C是一大类重要的蛋白磷酸酶,广泛参与不同的逆境胁迫响应。为了解PP2C基因在干旱响应中的功能,以亚洲棉石系亚1号为材料,利用RT-PCR方法从中克隆了GaPP2C24基因,并对该基因进行了生物信息学分析,荧光定量PCR研究了其在亚洲棉不同组织和干旱胁迫下的表达情况。结果表明,GaPP2C24基因CDS序列全长为1 251 bp,编码416个氨基酸。序列同源比对发现,GaPP2C24与其他植物的PP2C氨基酸序列有较高的相似性,其中与可可树(EOY33930.1)、黄麻(OMO91132.1)的相似性分别为85%,84%。进化分析表明,GaPP2C24与同属锦葵目的黄麻聚在一支,亲缘关系最近,与可可树的亲缘关系次之。实时荧光定量PCR结果表明,GaPP2C24基因在子叶、下胚轴、胚根、叶、茎和根均有表达,且在茎中的表达量最高,根中次之。GaPP2C24受干旱胁迫的诱导表达,其在根和叶片中的相对表达量在处理1 h后最高,分别达到对照的53.9,6.9倍,推测该基因在棉花干旱响应中有重要作用。  相似文献   

甘草黄酮对氧化损伤小鼠体内抗氧化作用的影响     

刘俊峰  刘永宏  郭雪峰 《塔里木大学学报》2015,(2):18-22

研究甘草黄酮对酒精氧化损伤小鼠体内MDA的含量及T-SOD的活力的影响;采用每天定时定量给小鼠灌胃不同浓度的甘草黄酮来研究甘草黄酮对氧化损伤小鼠体内MDA、T-SOD活力的影响。采用健康且体重相近的60只小白鼠,平均体重18±2 g,随机分为6组,每组10只。实验全期自由饮水,在第42d,分别取血清、肝脏和脑组织。实验测定对照组和实验组的小白鼠血清中的MDA的含量和T-SOD的活性。结果表明:实验组的T-SOD的活性显著高于对照组(P0.05),实验组的MDA的质量分数与对照组相比差异显著(P0.05)。  相似文献   

别墅侧院园林布景探析     

李永红 《安徽农学通报》2011,17(4):103-104,106

在园林布景中,别墅侧院是容易被忽视的角落。深入探讨别墅侧院园林布景的方法和技巧,有利于进一步促进别墅景观设计在理论和实践上的推陈出新。以图文并茂的形式进行解说和点评,旨在促使同行能更加直观的借鉴和引用。  相似文献   

网络时代高校图书馆信息资源建设研究     

郑永红 《计算机与农业》2011,(3):43-45

信息技术给高校图书馆的发展带来了机遇和挑战,信息资源已经成为高校图书馆馆藏资源的重要组成部分。从理论、观念、服务模式等方面阐述了信息时代高校图书馆信息资源建设应采取的措施。  相似文献