Determination of critical nitrogen dilution curve based on leaf area index for winter wheat in the Guanzhong Plain,Northwest China     

Sheng-cai QIANG  Fu-cang ZHANG  Miles Dyck  Yan ZHANG  You-zhen XIANG  Jun-liang FAN 《农业科学学报》2019,18(10):2369-2380

Excessive use of nitrogen(N) fertilizers in agricultural systems increases the cost of production and risk of environmental pollution. Therefore, determination of optimum N requirements for plant growth is necessary. Previous studies mostly established critical N dilution curves based on aboveground dry matter(DM) or leaf dry matter(LDM) and stem dry matter(SDM), to diagnose the N nutrition status of the whole plant. As these methods are time consuming, we investigated the more rapidly determined leaf area index(LAI) method to establish the critical nitrogen(N_c) dilution curve, and the curve was used to diagnose plant N status for winter wheat in Guanzhong Plain in Northwest China. Field experiments were conducted using four N fertilization levels(0, 105, 210 and 315 kg ha-1) applied to six wheat cultivars in the 2013–2014 and 2014–2015 growing seasons. LAI, DM, plant N concentration(PNC) and grain yield were determined. Data points from four cultivars were used for establishing the N_c curve and data points from the remaining two cultivars were used for validating the curve. The N_c dilution curve was validated for N-limiting and non-N-limiting growth conditions and there was good agreement between estimated and observed values. The N nutrition index(NNI) ranged from 0.41 to 1.25 and the accumulated plant N deficit(N_(and)) ranged from 60.38 to –17.92 kg ha~(-1) during the growing season. The relative grain yield was significantly affected by NNI and was adequately described with a parabolic function. The N_c curve based on LAI can be adopted as an alternative and more rapid approach to diagnose plant N status to support N fertilization decisions during the vegetative growth of winter wheat in Guanzhong Plain in Northwest China.  相似文献   

青稞粮草双高新品种藏青27的选育与栽培技术     

靳玉龙  周珠扬  白婷  强小林 《大麦与谷类科学》2017,34(3)

选育粮草双高型青稞新品种,既是西藏自治区粮食安全保障的要求,也是种植区农户种养结构改善的需要。藏青27以中晚熟高秆大粒丰产型新品种藏青320为母本,用中熟中秆大穗多粒的美国大麦品种做父本杂交,1987年形成杂交组合,2016年通过西藏自治区作物品种审定后推广。该品种在西藏全区多点生产比较示范中,平均产量为326.7 kg/667 m~2,比对照喜拉19号增产15.8%,丰产性较好。本文从选育过程、不同海拔种植地的播种期、播种管理、栽培等方面进行了阐述,以期为该品种的大面积推广提供参考。  相似文献   

奶牛繁殖疾病的调控     

黄正平  张慧林  刘小林 《畜牧兽医杂志》2009,28(1):52-55

本文综述了奶牛繁殖疾病的发病原因和调控措施，分析认为奶牛的营养状况是影响奶牛繁殖力的重要因素。理想的奶牛管理应做到整个泌乳期始终监测奶牛的体况和奶牛日粮中微量元素的维生素的含量，避免营养素的不平衡而引发的奶牛繁殖障碍，同时应避免奶牛过肥或过瘦。适当补充某些维生素和矿物质十分重要。  相似文献   

人血栓调节蛋白基因的表达及转基因小鼠的建立     

陈红英  苟克勉  王晓琳  陈学进  黄秀英  孙方臻 《中国兽医学报》2003,23(2):115-118

用PCR方法从人的基因组DNA中扩增了人血栓调节蛋白（hTM)基因，将其克隆到带有人EF－1α启动子的pEF-neo哺乳动物表达载体上，得到表达质粒pEF-TM。在转染pEF-TM的COS－1细胞中，hTM得到了瞬时表达，并且正确地定位到细胞膜上。用pEF-TM转染猪内皮细胞（PEC），经G418筛先得到稳定转染的克隆。凝血活性测定结果显示，表达hTM的PEC凝血时间明显延长，表明其抗凝血能力增强。通过显微注射方法，得到了1只F0代hTM转基因小鼠。Southern杂交结果表明，该转基因小鼠整合了9个拷贝的pEF-TM DNA,并能将外源DNA遗传给后代。  相似文献   

The Location Algorithm of QRS Waves Based on Fractal Box Dimensions Detection Methods     

LUO Xiao-gang  PENG Cheng-lin  ZHENG Xiao-lin  LI Qing-huiistry of Education  College of Bioengineering  Chongqing University  Chongqing  Chin 《保鲜与加工》2003,(12):51-54

We research fractal characteristics of ECG signal, and find that it is logarithmic linear relationship between the boxes number covering signal and box scale. It is shown that ECG signal have some characteristics. Further more, we discover that fractal dimensions at QRS site are higher than others when using a time windows to detect signal. Based on this foundation, we raise a location algorithm of QRS waves which is based on fractal boxes dimension detection methods. The application results of this algorithm show that it can get rid of noise in ECG effectively with high speed, so this algorithm can be used in real time detection of ECG signal.  相似文献   

and laminin on adhesion and proliferation of human hepatocellular carcinoma cell     

ZHANG Hai-wei  KUANG Min  CHEN Xiao-lin  ZHENG Pei-e 《园艺学报》2003,19(7):927-929

AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer.  相似文献   

Effect of moxonidine hydrochloride on left ventricular hypertrophy in spontaneously hypertensive rats     

ZHANG Peng-bo  NIU Xiao-lin  SUN Yu-ming  CHANG Dan  JINYao-feng 《园艺学报》2003,19(8):1089-1091

AIM:To investate the effect of domestric moxonidine hydrochloride on myocardium fibrosis and coronary artery microvascular structure in left ventricular hypertrophy of spontaneously hypertensive rats(SHR).METHODS:30 male SHR, aged 20 weeks, were divided into group Mox+SHR, Cap+SHR and SHR randomly (10 in each group). 10 age and sex-mached sprauge-dawley rats were designed as normal control(NC). At the end of 13 weeks, left ventricular wight/body weight ratio(LVW/BW), collagen volume fraction(CVF) and standardized perivascular collagen area(PVCA) as well as intramyocardial arterial average medial thickness (AMT) were determined.RESULTS:LVW/BW, CVF, PVCA and AMT in group Mox+SHR were lower significantly than that in group SHR, respectively.CONCLUSION:Long-term antihypertensive treatment with moxonidine hydrochloride reduces myocardium fibrosis and improves impaired coronary artery microvascular structure in left ventricular hypertrophy.  相似文献   

杏鲍菇优良品种HRX的选育研究   总被引：3，自引：1，他引：3  

王淑芳  卜庆梅  辛晓林  杨立红 《中国食用菌》2003,22(3):10-12

通过对杏鲍菇优质菌株HRX3定向驯化试验,结果表明:经过热处理的菌株,其对温度的耐受性能有明显改善;提高培养基中的木质素和纤维素成分,可以增强菇体内相关酶的活性;并筛选出适合本地栽培该菇的培养基质.  相似文献   

酯酶同工酶在食用菌菌种选育中的应用研究   总被引：3，自引：0，他引：3  

杨立红  辛晓林  蔡德华  谢红艳 《中国食用菌》2004,23(5):10-12

采用聚丙烯酰胺凝胶电泳 ,分析了不同培养时间和不同培养基种类对平菇、杏鲍菇、白灵菇酯酶同工酶的影响 ,结果表明在不同培养时间和不同培养基条件下 ,酯酶同工酶酶谱的酶带呈现不同的多态性 ,由此提示同工酶在食用菌菌种选育的应用上应固定最佳培养时间及最适培养基种类 ,并在所有操作条件一致的情况下 ,才能得到稳定准确的分析结果  相似文献   

胶孢炭疽菌株QZ-97a分生孢子货架期及对温度的耐受力     

朱秦  强胜 《中国生物防治》2004,20(2):122-126

将不同含水量的胶孢炭疽菌婆婆纳专化型菌株QZ 97a分生孢子粉置于4℃和室温下,密闭或开放贮存。结果表明,4℃下密闭贮藏6个月,分生孢子萌发率和芽管长度无明显降低,室温贮藏降低也不明显。贮藏10个月后,各种贮藏方式孢子的活力都显著下降。但以4℃、含水量4%、密闭贮藏孢子活力较为稳定,420d孢子萌发率为70 4%,芽管长度为贮藏60d时的43 2%。含水量9 6%、开放室温下贮藏的孢子粉活力下降最大。因此,温度和孢子粉含水量是影响孢子货架期的两个主要因素。分生孢子粉对温度有一定的耐受性,随温度增高,敏感性增强,60℃下分生孢子的活力下降显著,处理6min,孢子芽管长度较对照下降50%。  相似文献