鸡霍乱口服弱毒疫苗的研究     

刘学贤  丁铲  于圣青  尤兆岚 《中国预防兽医学报》1997,(5)

用经过选择的禽多杀巴氏杆菌自然弱毒菌株Ｒ１－２３菌株制成的鸡霍乱固体培养口服弱毒疫苗，其安全剂量接近于２００个免疫剂量。该口服苗两次饮水免疫的最适总剂量为每羽５０亿个活菌，最佳间隔时间为４８小时。免疫鸡第二次饮苗后第３天即可产生较好的免疫保护率（７／８）。免疫鸡对异型强毒株Ｐ１０５９（８∶Ａ）、Ｐ２７２３（９∶Ａ）和同型强毒株Ｃ４８－１（５∶Ａ）滴鼻攻击的近期保护率平均为５／１０、６／１０和９／１０。６个月免疫期的平均保护率为７６．７０％（４６／６０），一万多羽鸡田间试验结果表明，免疫后２个月的平均保护率为９３．１０％（２７／２９），６个月的平均保护率为７３．５０％（２５／３４），除对产蛋率有轻度影响（下降１．２７％）外，无其它不良反应。野外大面积使用结果表明，该口服苗不但性能稳定，免疫原性优良，而且安全可靠，适用于鸡口服免疫。  相似文献   

Pyruvate kinase is necessary for Brucella abortus full virulence in BALB/c mouse     

Jianpeng Gao  Mingxing Tian  Yanqing Bao  Peng Li  Jiameng Liu  Chan Ding  Shaohui Wang  Tao Li  Shengqing Yu 《Veterinary research》2016,47(1)

Brucellosis, caused by a facultative intracellular pathogen Brucella, is one of the most prevalent zoonosis worldwide. Host infection relies on several uncanonical virulence factors. A recent research hotpot is the links between carbon metabolism and bacterial virulence. In this study, we found that a carbon metabolism-related pyruvate kinase (Pyk) encoded by pyk gene (locus tag BAB_RS24320) was associated with Brucella virulence. Determination of bacterial growth curves and resistance to environmental stress factors showed that Pyk plays an important role in B. abortus growth, especially under the conditions of nutrition deprivation, and resistance to oxidative stress. Additionally, cell infection assay showed that Pyk is necessary for B. abortus survival and evading fusion with lysosomes within RAW264.7 cells. Moreover, animal experiments exhibited that the Pyk deletion significantly reduced B. abortus virulence in a mouse infection model. Our results elucidated the role of the Pyk in B. abortus virulence and provided information for further investigation of Brucella virulence associated carbon metabolism.  相似文献   

Isolation of myxoviruses from migratory waterfowls in San-in district,western Japan in winters of 1997-2000     

Shengqing Y  Shinya K  Otsuki K  Ito H  Ito T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):1049-1052

Between November 1997 and February 2000, winter migratory waterfowls of several species staying in San-in district, western Japan were surveyed for influenza A virus and paramyxovirus at four stations. A total of 18 influenza A viruses was isolated from 1,404 fecal samples of whistling swans, pintails, mallards, and white-fronted geese. Five different hemagglutinins and eight neuraminidases were identified in the viruses isolated, in 11 different combinations, including H7N8 related to a subtype of a highly pathogenic chicken virus. In 2000, five lentogenic (non-pathogenic) Newcastle disease viruses were also isolated from white-fronted geese. These results suggested that possible precursor viruses for highly pathogenic avian myxoviruses are still brought into Japan by migratory waterfowls. The results also support the contention that continued surveillance of wild waterfowl population should be an integral part of control policies for these serious poultry diseases.  相似文献   

毛白杨纤维素合酶基因(PtoCesA1)的克隆及其在烟草中的遗传转化   总被引：1，自引：0，他引：1  

李春秀  齐力旺  史胜青  汪阳东  王建华  张守攻 《林业科学》2007,43(3):39-45

克隆毛白杨纤维素合酶基因(PtoCesA1),全长为3 215 bp,与欧洲颤杨的PtrCesA1基因的同源性为97%,具有开放的阅读框,编码区在52～2 988碱基之间,为2 937 bp.构建PtoCesA1基因的全长正义植物表达载体为pBIPA1,经酶切和PCR鉴定确认载体构建正确.通过农杆菌介导的方法将pBIPA1表达载体转入烟草中,转基因植株的纤维细胞壁厚度和木质部厚度都有不同程度的下降,形态表征为植株明显变矮,叶片变小.  相似文献   

海拔与加施有机肥对香榧果园土壤肥力的影响     

吴远海  臧运祥  吴陆斌  刘伟  吴盛清  张婷婷  毛永铨 《湖南林业科技》2022,49(2):20-24,31

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The siderophore-interacting protein is involved in iron acquisition and virulence of Riemerella anatipestifer strain CH3     

Jing Tu  Fengying Lu  Shuang Miao  Xintao Ni  Pan Jiang  Hui Yu  Linlin Xing  Shengqing Yu  Chan Ding  Qinghai Hu 《Veterinary microbiology》2014,168(2-4):395-402

Riemerella anatipestifer causes epizootic infectious disease in poultry and serious economic losses especially to the duck industry. However, little is known regarding the molecular basis of its pathogenesis. The ability to acquire iron under low-iron conditions is related to the virulence of a variety of bacterial pathogens. In this study, a sip (Riean_1281) deletion mutant CH3Δsip was constructed and characterized for iron-limited growth, biofilm formation, and pathogenicity to ducklings. Results showed that siderophore-interacting protein (SIP) was involved in iron utilization and the sip deletion significantly reduced biofilm formation and adherence to and invasion of Vero cells. In addition, the sip gene was absent in 1 of 24 (4.17%) virulent strains and 2 of 3 (66.7%) avirulent strains of R. anatipestifer, and the sip gene from six R. anatipestifer strains, which belong to serotypes 1, 2, and 10, respectively, shared 100% amino acid identities to those of R. anatipestifer strains DSM15868 and RA-GD. These results suggested that siderophore-mediated iron acquisition may be an important iron-uptake pathway in R. anatipestifer. Animal experiments indicated that the median lethal dose of the CH3Δsip mutant in ducklings was about 35-fold higher than that of the wild-type CH3 strain. Thus, our results demonstrated that R. anatipestifer SIP was involved in iron acquisition and necessary for its optimal virulence.  相似文献   

单克隆抗体夹心ELISA试验检测鸡新城疫病毒抗原的研究   总被引：1，自引：0，他引：1  

于圣青  刘秀梵 《扬州大学学报(农业与生命科学版)》1990,(2)

从10株抗鸡新城疫病毒(NDV)特异性单克隆抗体(MAbs) 中筛选出2株MAbs—M22和F23,分别用作包被抗体和酶标抗体而建立的MAbs—夹心ELISA试验,对提纯NDV的最小检出量为25ng/ml病毒蛋白.NDV鸡阳性血清对本试验的特异性阻断作用和与其他禽类病毒交叉反应的阴性结果,证明本试验对NDV的特异性。从临床疑为鸡新城疫的724只病鸡的口腔棉试样品中共检出阳性502只,其中200个样品与病毒分离试验结果完全一致,两者均查出阳性鸡176只。  相似文献   

虱螨脲80%水分散粒剂的研制     

刘兵  戴航星  尹旺华  陈胜庆  陈其平  刘庆华 《农药科学与管理》2012,33(11):12-15

虱螨脲属高效低毒的苯甲酰脲类杀虫剂,主要用于防治玉米、蔬菜、棉花、果树等鳞翅目等幼虫;另外,虱螨脲也可用于防治宠物身上和公共场所的跳蚤[1]。我们通过该药的特点,对其水性化高含量农药制剂进行了研究,并确定了最佳配方。对该产品的崩解性、悬浮率、分散性、润湿性和稳定性进行了测定。研究结果表明产品各项技术指标和热贮稳定性测定均达到水分散粒剂的质量指标。  相似文献   

血清10型鸭疫里默氏杆菌灭活油乳剂疫苗的研制     

王小兰  刘蕾  刘海文  苗双  胡青海  韩先干  丁铲  于圣青 《中国家禽》2012,34(15):29-32

鸭疫里默氏杆菌(Riemerella anatipestifer,RA)感染是主要危害2~8周龄雏鸭的高致病性细菌性传染病。为研制血清10型RA灭活油乳剂疫苗,选取10株血清10型RA分离株进行动物体复壮、分离和鉴定后,分别对部分毒株进行了毒力测定和灭活油乳剂疫苗的研制。结果表明,所有试验用分离株对7日龄雏鸭均具有较强的致病力。分离株YXL1和HXb2的半数致死量分别为2.8×108 cfu和82 cfu,不同菌株之间的毒力差异很大。用研制的灭活油乳剂疫苗对雏鸭进行2次免疫后,免疫鸭可获得高水平的特异性抗体,并可抵抗强毒RA的攻击,以HXb2免疫后的攻毒保护性最好。抗体水平检测和攻毒保护试验结果表明,用血清10型RA分离株HXb2制备的油乳剂灭活疫苗具有良好的免疫保护作用。  相似文献   

应用PAPD方法对鸡败血霉形体DNA多态性的研究   总被引：2，自引：0，他引：2  

于圣青  丁铲  周怀军  刘晓文  徐步 《中国兽医学报》1999,19(3):261-263

用随机扩增多态性ＤＮＡ（ＲＡＰＤ）方法,通过筛选的５个随机引物（ＯＰＨ－０２,ＯＰＨ－０５,ＯＰＨ－０７,ＯＰＨ－１３,ＯＰＧ－１６）,对１４株鸡败血霉形体（ＭＧ）国际标准株和国内分离株进行了ＤＮＡ多态性研究。结果表明,５个引物共产生２１种条带,其中有３个条带分别为２个菌株所特有,扩增产物片段的长度在１５０～４５００ｂｐ之间,所有菌株均有１条共同条带,以ＯＰＨ－０５扩增产物的多态性最丰富。根据样品ＤＮＡ所获得的菌株间相似性指数表明,Ｄ９６０３与Ｄ９６０７相似指数最高,Ｓ６,Ｋ３９１３和Ｄ９６０４三者相互间相似性指数最低。提示ＲＡＰＤ方法可用于霉形体遗传标记的分析。  相似文献