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1.
Genetic Resources and Crop Evolution - Cicer arietinum L. (chickpea) is one of the most significant legume crops domesticated in the Fertile Crescent. This study was aimed to characterize a diverse...  相似文献   
2.
Kappaphycus alvarezzi, an edible seaweed from the west coast of India, was analyzed for its chemical composition. It was found that K. alvarezzi is rich in protein (16.24% w/w) and contains a high amount of fiber (29.40% w/w) and carbohydrates (27.4% w/w). K. alvarezzi showed vitamin A activity of 865 mug retinal equivalents/100 g of sample. It contained a higher quantity of unsaturated fatty acids (44.50% of the total), in which relative percentage of oleic acid was 11%, cis-heptadecanoic acid 13.50%, and linoleic acid 2.3% and 37.0% of saturated fatty acids (mainly heptadecanoic acid). K. alvarezziwas also found to be good source of minerals, viz 0.16% of calcium, 0.033% of iron, and 0.016% of zinc, which are essential for various vital biological activities. Bioavailability of iron by in vitro methods showed a higher efficiency in intestinal conditions than in stomach conditions. Ascorbic acid influenced higher bioavailability of iron. Successive extracts of n-hexane, acetone, ethyl acetate, ethanol, and direct extractables of chloroform/methanol (1:1 and 2:1) were screened for antioxidant activity using a beta-carotene linoleic acid model system (B-CLAMS), DPPH (alpha,alpha-diphenyl-beta-picrylhydrazyl) model system and hydroxyl radical scavenging activity. The chloroform/methanol (2:1) extract has shown 82.5% scavenging activity at 1000 ppm. Acetone fraction extracts at the 1000 ppm level showed 63.31% antioxidant activity in beta-carotene linoleic acid system. The acetone extract showed 46.04% scavenging activity at 1000 ppm concentration. In the case of hydroxyl radical scavenging activity, all the extracts showed better activity at the concentrations of 25 and 50 ppm, where at the 50 ppm level ethyl acetate extract showed 76.0%, acetone 75.12%, and hexane 71.15% activity, respectively. Results of this study suggest the utility of K. alvarezzi (Eucheuma) for various nutritional products, including antioxidant for use as health food or nutraceutical supplement.  相似文献   
3.
The anthelmintic potentials of the aqueous and methanol extracts of Euphorbia helioscopia were investigated. In folklore medicine, E. helioscopia (Euphorbiaceae) is used in the treatment of various gastrointestinal ailments and diseases. A worm motility inhibition (WMI) assay and egg hatch assay (EHA) were used for in vitro assessment, and a faecal egg count reduction (FECR) assay was used for an in vivo study. The in vitro study revealed anthelmintic effects of crude methanolic extracts of E. helioscopia on live Haemonchus contortus worms as evident from their paralysis and/or death at 8h after exposure, different concentrations (12.5mgml(-1), 25mgml(-1) and 50mgml(-1)) of aqueous and methanolic extracts were used against H. contortus which exhibited dose-dependent anthelmintic effects on H. contortus. Aqueous and methanolic extracts of aerial parts (stem, leaves and flowers) of E. helioscopia were found to have very low percent inhibitory egg hatching as compared to levamisole. It is concluded that the entire plant of E. helioscopia possesses significant anthelmintic activity and could be a potential alternative for treating cases of helminth infections in ruminants.  相似文献   
4.
In order to assess the species richness and diversity profile of helminth parasite fauna in an endemic fish, an investigation was carried out in two urban and two rural lakes of Kashmir. Overall nine species of helminth parasites were observed in four lakes. Of these three were autogenic and six were allogenic. Heteroxenous parasite species were more in number than monoxenous species. Results showed significant differences in heteroxenous / monoxenous ratio between different lakes. Core species (Prevalence?>?20) were only found in hypertrophic lake (Anchar Lake). Overall, majority of helminth species were either secondary or satellite species. Prevalence of some helminth parasites showed significant differences in different lakes. In addition mean intensity showed significant differences between autogenic and allogenic parasites (P?<?0.05). Principle Component Analysis based on prevalence showed that Anchar Lake was strongly associated with most of helminth parasites. Diversity indices showed significant variation between different lakes. Maximum helminth species per host was in Anchar Lake. Finally we concluded that helminth parasite fauna showed significant differences in species richness and infection indices between different lakes. Diversity profile was higher in Anchar Lake in comparison to other three lakes. The results clearly show that environmental features of lake ecosystems have got an impact on distribution pattern of helminth parasites in S. esocinus. We suggest comparative parasitological study should be taken between different species of fish in order to have a clear picture regarding the species composition of helminth species in this region. Also we need to characterize the species spectrum of parasitic worms in fish of freshwater bodies of this region as well as other similar type of climatic zones because parasite fauna is an integral part of the inventory of biodiversity and as possible regulators of host populations in aquatic ecosystems.  相似文献   
5.
Detailed chemical studies and RAPD analysis were done in different populations of Podophyllum hexandrum collected from high altitude regions of North Western Himalayas. Random amplified polymorphic DNA (RAPD) analysis revealed a high degree of genetic diversity among the 12 collected accessions, attributed to their geographical and climatic conditions. HPLC analysis also revealed variation in the concentration of two major marker compounds which lead to the identification of a chemotype. The study demonstrated that RAPD and chemical markers are very useful tools to compare the genetic relationship and pattern of variation among such prioritized and endangered medicinal plants.  相似文献   
6.
The response of nursery seedlings and wildings of oriental beech(Fagus orientalis Lipsky) to canopy gap size was studied ina climax beech forest in northern Iran with respect to seedlingsurvival, shoot growth and vitality. These parameters were followedfor 2 years after planting in gaps of 50, 200 and 600 m2, aswell as in the open field. Foliage coloration was used as acriterion for vitality. The survival rate of nursery seedlingsand wildings dropped rapidly with increasing gap size from 84per cent in 50-m2 canopy openings to  相似文献   
7.
Seventy-five Escherichia coli isolates with at least one targeted virulence gene were recovered from 338 lambs with (n=230) and without (n=108) diarrhoea. The isolates belonged to 36 different serogroups. Shiga toxin-producing E. coli (STEC) was isolated from 9.6% of lambs with and 24.1% of lambs without diarrhoea. Enteropathogenic E. coli (EPEC) was isolated from 6.1% of lambs with and 11.1% of lambs without diarrhoea. Of 26 EPEC isolates, seven were typical (positive for bfpA), and, of 34 stx(1) positive isolates, 25 were subtyped as stx(1c). Five of 29 stx(2) positive isolates were subtyped as stx(2d) and two as stx(2c). Seven of 45 eae positive isolates were subtyped as eae subtype zeta (eaezeta). This appears to be the first report of the isolation of typical EPEC from sheep in India.  相似文献   
8.
To achieve higher titer of rabies virus higher density of host cells will need. In this study, capability of FibraCel disks packed in 500 mL spinner basket versus Cytodex-1 in 500 mL spinner flask was investigated for propagation of Vero cells and PV rabies virus proliferation. Minimal Essential Medium (MEM) + 10% Foetal Calf Serum (FCS) and Virus Production- Serum Free Medium (VP-SFM) +4 mM L-glutamine were used in growth phase and MEM+ 0.2% Bovine Serum Albumin (BSA) and VP-SFM were used in virus production phase. Adapted Vero cells grown in VP-SFM were used in all SFM experiments while batch and stepwise perfusion modes were applied and compared in growth stage. The highest Vero cell density were achieved in the trials with 10 g FibraCel disk in stepwise perfusion mode equal to 6.12 x 10(6) and 5.87 x 10(6) cells mL(-1) in MEM and VP-SFM, respectively while with 2.73 g Cytodex-1 lower density equal to 4.2 x 10(6) and 4.0 x 10(6) cells mL(-1) were achieved. The highest titer of rabies virus and overall virus production rate were resulted in VP-SFM and on 10 g disks equal to 2.9 x 10(7) Fluorescent Focus Unit (FFU) mL(-1) and 0.14 FFU/Cell/h, respectively versus 1.7 x 10(7) FFU mL(-1) and 0.08 FFU/cell/h on cytodex-1 in similar conditions. The second harvest of virus was also satisfactory in experiment with 10 g disks (1.7 x 10(7) FFU mL(-1)) in compare to Cytodex-1 (0.51 x 10(7) FFU mL(-1)). An equal surface area at 6600 and 12000 cm(-2) were provided in all comparable trials with seeding density of 12.5 x 10(3) cells cm(-2). Adapted Vero cells grown in VP-SFM were used in all SFM experiments while batch and stepwise perfusion modes were applied and compared in growth stage.  相似文献   
9.
10.
This study was undertaken to compare patterns of agronomic characters and yellow pigment content diversity between Iranian durum landraces and modern varieties. In four field experiments, a collection of 127 accessions of landraces and 23 modern cultivars tetraploid wheat representing Iranian and global genetic diversity were tested under optimum and rain-fed conditions. Across the environments, the yellow pigment concentrations ranged from 1.85 mg kg?1 to 8.95 mg kg?1 in the field-grown samples. Multivariate analysis detected five groups, four including landraces and one comprising modern cultivars. As a group, modern cultivars were the most productive and showed high mean values for harvest index, grains per spike and yellow pigment, but they had the lowest plant height. Landraces had the highest mean number of spikes per m2 and 1000-kernel weight, but were characterized by low plant height and yellow pigment content. Clustering analysis also showed that, based on yield and yellow pigment content, the accessions in different groups were of different origins, suggesting that there was no clear relationship between accessions and geographical diversity. According to our results, Iranian landraces can be particularly useful as germplasm in breeding programmes to improve spikes per m2 and 1000-kernel weights.  相似文献   
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