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利用C基因组对高杆野生稻和宽叶野生稻基因组的比较分析 总被引:1,自引:0,他引:1
[目的]采用基因组原位杂交(GISH)技术研究稻属2种CCDD基因组型的野生稻宽叶野生稻和高杆野生稻基因组之间的关系。[方法]利用药用野生稻C基因组总DNA为探针,分别对高杆野生稻和宽叶野生稻中期染色体进行基因组原住杂交。[结果]杂交结果显示,在一定的洗脱严谨度下,可以把CCDD染色体组中的C、D基因组染色体分开,并且发现高杆野生稻的CCDD基因组中的某些属于CC基因组的染色体与宽叶野生稻和药用野生稻中的CC基因组染色体存在较大差异,宽叶野生稻的基因组更加原始。[结论]对稻属中有相同基因组型的种进行比较分析,将有助于深入阐明植物基因组进化和物种进化及可能的途径。 相似文献
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利用C基因组对高杆野生稻和宽叶野生稻基因组的比较分析(英文) 总被引:1,自引:0,他引:1
[Objective] Genomic in situ hybridization (GISH) was used to study the relationship between the two CCDD genomes of Oryza alta and Oryza latifolia. [Method] Total DNA of Oryza officinalis (C-genome) was used as a probe for genomic in situ hybridization on metaphase chromosomes from Oryza alta and Oryza latifolia, respectively. [Result] Under certain post-hybridization washing stringencies, C- and D-genome could be distinguished in CCDD genome type; there were huge differences in some CC chromosomes of Oryza alta, Oryza latifolia, and Oryza officinalis. The genome of Oryza latifolia was more original. [Conclusion] Comparative analysis of the Oryza species with identical genome type may facilitate to elucidate the possible approaches to plant genome evolution and species evolution. 相似文献
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<正>1.旋耕机负荷过大。原因:耕深过度,土壤粘重、过硬造成。排除:可减少深耕,降低机组前进速度和犁刀的转速。2.旋耕机工作时跳动。原因:土壤坚硬或刀片安装不正确。排除:可降低机组前进速度和犁刀转速,并正确安装刀片。3.旋耕机间断抛出大土块。原因:刀片弯曲变形,刀片折断、丢失或严重 相似文献
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