全文获取类型
收费全文 | 1081篇 |
免费 | 54篇 |
国内免费 | 116篇 |
专业分类
林业 | 122篇 |
农学 | 166篇 |
基础科学 | 102篇 |
180篇 | |
综合类 | 291篇 |
农作物 | 35篇 |
水产渔业 | 54篇 |
畜牧兽医 | 185篇 |
园艺 | 42篇 |
植物保护 | 74篇 |
出版年
2024年 | 3篇 |
2023年 | 12篇 |
2022年 | 43篇 |
2021年 | 45篇 |
2020年 | 44篇 |
2019年 | 48篇 |
2018年 | 30篇 |
2017年 | 35篇 |
2016年 | 47篇 |
2015年 | 43篇 |
2014年 | 42篇 |
2013年 | 55篇 |
2012年 | 75篇 |
2011年 | 63篇 |
2010年 | 64篇 |
2009年 | 54篇 |
2008年 | 52篇 |
2007年 | 60篇 |
2006年 | 44篇 |
2005年 | 59篇 |
2004年 | 26篇 |
2003年 | 19篇 |
2002年 | 17篇 |
2001年 | 15篇 |
2000年 | 28篇 |
1999年 | 32篇 |
1998年 | 25篇 |
1997年 | 26篇 |
1996年 | 25篇 |
1995年 | 21篇 |
1994年 | 14篇 |
1993年 | 20篇 |
1992年 | 20篇 |
1991年 | 12篇 |
1990年 | 10篇 |
1989年 | 10篇 |
1988年 | 4篇 |
1987年 | 2篇 |
1985年 | 2篇 |
1981年 | 1篇 |
1978年 | 1篇 |
1975年 | 1篇 |
1965年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有1251条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
利用黄淮地区的12个高产夏大豆品种(系),通过NCⅡ交配设计,对12个主要农艺性状的遗传进行了分析。结果表明,所有性状的加性方差大于显性方差。主茎节数、株高、每簇荚数和主茎荚数的加性成分占绝对优势,遗传力较高。分枝荚数、单株粒数和单株粒重的显性成分比例大,遗传力较低,其中单株粒重趋于完全显性。主茎节数的显性成分很少,其它性状均为部分显性。建立了单株产量与产量因子之间的逐步回归方程。 相似文献
5.
农户舍饲奶牛血清钙及游离羟脯氨酸水平测定 总被引:2,自引:0,他引:2
采用氯胺T比色法和EDTANa_2滴定法,对重庆市农户舍饲奶牛不同泌乳阶段血清中游离羟脯氨酸与血清钙的水平进行 测定,旨在调查试验条件下奶牛血钙代谢情况以及骨营养不良发生的可能性。结果显示: 在农户全舍饲条件下,血清钙含量Ⅱ组显著低于Ⅴ组、Ⅵ组,其它各组间差异不显著;Ⅰ~ Ⅵ组血清羟脯氨酸含量分别为2.32±0.37μg/ml、2.36±0.59μg/ml、2.02±0.54μg/ml、1.90±0.44μg/ml、1.98±0.45μg/ml、1.79±0.18μg/ml,Ⅰ、Ⅱ组与Ⅵ组差异极显著。采样后2-3个月内进行临床观察,血清游离羟脯氨酸水平与骨营养不良发生率呈现一致性变化。而钙磷摄入不足,比例不当是主要原因。 相似文献
6.
7.
Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats 下载免费PDF全文
Sui Zhi Tian Chai Ju Chang Chih Chi Chiang Huo Cheng Peh Mu Chiou Huang Jai-Wei Lee Xin Zhao 《Canadian journal of veterinary research》2005,69(1):39-45
The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis. 相似文献
8.
农杆菌介导的蝴蝶兰基因转化系统的建立 总被引:9,自引:1,他引:9
针刺后的蝴蝶兰‘White Hikaru’的类原球茎(PLB),与含绿色荧光蛋白基因( )和潮霉素磷酸转移酶基因(^p£)的pCAMBIA 1300一SmGFP的根瘤农杆菌LBA4404 共培养,培育出了转基因的蝴蝶兰。经绿色荧光蛋白检测和Southern印迹,证实了再生植株中含cop基因和hpt基因。 相似文献
9.
Comparative expression profile of microRNAs and piRNAs in three ruminant species testes using next‐generation sequencing 下载免费PDF全文
microRNA (miRNA) and piwi‐interacting RNA (piRNA) are two classes small non‐coding regulatory RNAs that play crucial roles in multiple biological processes such as spermatogenesis. However, there are no published studies on conjoint analysis of miRNA and piRNA profiles among cattle, yak and their interspecies (the dzo) using sequencing technology. Next‐generation sequencing technology was used to profile miRNAs and piRNAs among those three ruminants to elucidate their functions. A total of 119, 14 and six differentially expressed miRNAs were obtained in cattle vs. dzo, cattle vs. yak and yak vs. dzo comparison groups, while there were 873, 1,065 and 1,158 differentially expressed piRNAs in those three comparison groups. The expression of three miRNAs was validated in the three ruminants, and the results suggested that the miRNA expression profiles data could represent actual miRNA expression levels. Moreover, the putative targets of differentially expressed miRNAs were predicted by their own genome, it is worth to note that both the cattle and yak genome were used for dzo, then the targets were subjected to GO enrichment and KEGG pathway analysis, revealing the likely roles for these differentially expressed miRNAs in spermatogenesis. In conclusion, this study provided a useful resource for further elucidation of the miRNAs and piRNAs regulatory roles in spermatogenesis. It may also facilitate the development of therapeutic strategies for dzo reproduction research. 相似文献
10.