Acute and reproductive toxicity of Annona squamosa to Aedes albopictus     

Vivek Kempraj 《Pesticide biochemistry and physiology》2011,100(1):82-86

The current study involved evaluation of the toxicity of acetone soluble fraction of the ethanol extract of Annona squamosa Linn. seeds on Aedes albopictus (Skuse). Toxicity of fresh sample has been compared with that of solar radiated and heat treated aliquots of the same. Acute toxicity of fresh acetone fraction on adults was evident with LC₅₀ and LC₉₀ values of 15.21 and 60.38 μg/ml, respectively. Larvicidal bioassays recorded LC₅₀ and LC₉₀ values ranging from 0.44 to 5.97 and 1.64-43.36 μg/ml, respectively for different instars. Ovicidal bioassays yielded EC₅₀ and EC₉₀ values of 18.82 and 69.61 μg/ml. The study further revealed ovipositional deterrent and chemosterilant activities of the extract on the target mosquito. Bioassays using solar radiation and heat treated samples of the active fraction have showed toxicity levels similar to those of fresh sample. Chemical analysis of acetone soluble fraction of seed extract of A. squamosa has reveled ethyl oleate and iso-octyl phthalate as major components. Adulticidal, larvicidal, ovicidal, ovipositional deterrent and chemosterilant activities of the fraction on A. albopictus were proved. The investigation further confirmed stability of the active fraction on exposure to solar radiation and high temperature.  相似文献   

奶牛乳腺炎金黄色葡萄球菌TH-16S 733330株荚膜多糖免疫原性研究     

马文戈  李建军  喻昌盛  徐志光 《中国兽药杂志》2006,40(2):26-28

从新疆分离的奶牛乳腺炎金黄色葡萄球菌,经TH-16S系统鉴定,选取鉴定结果为733330的优势生化株。毒力测定试验表明,1×108CFU/只的接种量会使小白鼠在24 h内死亡。菌株接种Columbia Broth Modified 37℃摇床100 r/m in培养24 h后,用0.05%戊二醛灭活。灭活培养物经4℃30 m in 17 000 g离心去除细胞碎片,上清用PBS(pH 7.2)4℃透析48 h,PEG20000浓缩10倍,透析,加入终浓度为10 mg/mL的蜂胶-乙醇浸液,作为荚膜多糖免疫原。先分别用荚膜多糖免疫原0.1、0.2、0.4 mL免疫小白鼠,再用组合免疫原(荚膜多糖+109CFU/mL菌体)0.1、0.2、0.4 mL免疫小白鼠。6组小鼠于免疫后14 d连同对照小鼠2组攻击金黄色葡萄球菌2×108CFU/(0.2 mL.只)。结果表明,荚膜多糖免疫原组保护率分别为0、25%和50%,(荚膜多糖+菌体)组保护率分别为25%、75%和100%。  相似文献   

灭幼脲一号致粘虫成虫产卵不育的研究——室内不育研究部分   总被引：1，自引：0，他引：1  

赵文臣  焦惠生  张淑芬 《华北农学报》1986,(2)

利用化学药剂导致害虫不育是防治害虫的一个新途径.1980年以来笔者用灭幼脲一号乳剂采用Mitlin饲食法于室内对粘虫成虫产卵不育做了研究.试验结果表明,用5oppm处理羽化后一天内的雌、雄蛾,交配后所产卵,不育效果达99.8%,几乎全部不育.用5ppm、50ppm同时处理羽化后第三天雌,雄蛾,交配后所产卵,不育效果分别为99.4—99.0%,9.96—99.1%,效果十分明显.不育效果似与成虫羽化天数无明显关系.这一研究结果,对控制迁飞性昆虫为害及其数量变动具有重要意义和实用价值.  相似文献   

EP-1粒不育剂筛选与配制应用     

舒东辉  李春晓 《内蒙古林业调查设计》2012,35(5):96-97,111

文章介绍了EP-1新型颗粒不育剂药饵适宜组成及加工工艺流程,药量、药效的筛选以及模拟自然的药效试验,为不育剂调控林木害鼠棕背平繁殖找到新途径。  相似文献