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以2个高粱(Sorghum bicolor L.)亲本纯系及其F1杂交种为材料,利用甲基化敏感扩增多态性(MSAP)方法,对DNA甲基化水平和模式的遗传和变异进行了研究.结果表明:亲本纯系的大部分胞嘧啶甲基化位点稳定遗传给其F1杂交种,但3.94%的位点表现出了变异,即未从亲本遗传给杂交种,并通过Southern杂交方法进一步验证了杂交种和纯系亲本间的DNA甲基化模式的遗传和变异. 相似文献
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急性病毒性坏死病毒引物酶表达及酶学活性分析 总被引：1，自引：1，他引：0
根据急性病毒性坏死病毒(acute viral necrosis virus,AVNV)ORF 024序列设计引物,以AVNV的DNA为模板进行扩增AVNV引物酶(primase)基因,同时构建表达质粒pET32a-prim,并转化至E.coli BL21(DE3)进行诱导表达。SDS-PAGE检测显示诱导表达两条蛋白条带,其中分子量为60 ku的蛋白条带与预期表达蛋白条带大小一致,另一蛋白条带分子量约为55 ku,经Western-blotting及质谱分析鉴定分子量60 ku的蛋白条带为AVNV-引物酶,而表达出的55 ku蛋白条带存在部分引物酶多肽片段。RNA结合荧光染料Pico-Green在30 min内荧光强度比较稳定,从而确定30 min为进行AVNV引物酶活性分析的最佳终止反应时间,并在引物合成实验中观察到30 min内引物酶活性较高。当使用多聚胞嘧啶寡核苷酸poly(d C)为模板时,重组引物酶能特异性催化底物GTP。0.1 mmol/L Zn2+可显著增强引物酶活性,而1mmol/L Mn2+和EDTA能抑制引物酶活性。 相似文献
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Global Analysis of Cytosine Methylation and Proteome Under Cold Treatment in Brassica napus 
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下载免费PDF全文 WEI Fang ;HU Jie ;CUI Ming-zhu ;ZHANG Yan-hui ;LI Yun-ling ;TIAN Bao-ming 《农业科学学报》2014,13(10):2170-2176
Cytosine methylation/demethylation plays pivotal roles in regulating gene expression at a genome-wide level. However, limited reports are available to reveal correlating changes of cytosine methylation and proteomic expression in Brassica napus so far. Therefore, in the present study, global cytosine methylation and proteome were analysed in B. napus after cold treatment by methylation-sensitive amplified polymorphism （MSAP） and two-dimensional protein electrophoresis technology （2-DE）. The results showed that the lowered genome-wide DNA methylation status was revealed after cold treatment, and about 0.88% of discrepancy in DNA methylation was detected between the non-flowering and flowering plants after cold treatment. Moreover, the 52 significantly up-regulated proteins emerged in comparison with the 36 down-regulated proteins, as well as the 14 proteins exclusively detected in the flowering plants. Intriguingly the 8 specifically expressed proteins in the non-flowering plants disappeared in the flowering plants with cold treatment. Therefore, these present data proved that the correlating changes of cytosine methylation and proteomic expression were evidenced under cold treatment in B. napus. 相似文献
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正犬真菌性皮肤病是由犬小孢子菌、石膏样小孢子菌及马拉色菌等致病性真菌黏附于犬皮肤上,侵入皮肤表皮、毛发或趾甲从而引起皮肤的一系列器质性病变,是犬临床最常见的传染性皮肤疾病之一。抗真菌药(antifungal drugs)是治疗该病的有效药物,其作用机制在于损害细胞膜、损害真菌细胞壁以及影响真菌核酸合成。传统抗真菌药 相似文献
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DNA甲基化抑制剂作用机理及其在植物发育生物学研究中的应用 总被引：4，自引：0，他引：4
本文综述了DNA甲基化抑制剂作用机理及其在植物发育生物学研究中的应用。DNA甲基化抑制剂使甲基基团不能转移到腺嘌呤或胞嘧啶,导致DNA甲基化反应受阻,从而使基因组甲基化水平降低。DNA甲基化抑制剂已被用于因DNA甲基化引起的植物表观遗传研究中,同时它可以代替低温促进植物提早开花,并可能在植物性状改良中得到进一步的研究和应用。 相似文献
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免疫增强剂可促进动物对抗原的免疫应答反应,显著增强疫苗的免疫效果。文章综述了动物疫苗研究领域中的免疫增强剂,如免疫刺激复合物、脂质体、细胞因子、胞嘧啶-鸟嘌呤寡脱氧核苷酸和天然产物对动物疫苗免疫效果的影响,并对免疫增强剂的发展趋势作了展望。 相似文献
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Study on DNA Cytosine Methylation of Cotton （Gossypium hirsutum L.） Genome and Its Implication for Salt Tolerance 总被引：1，自引：0，他引：1
ZHAO Yun-lei YU Shu-xun YE Wu-wei WANG Hong-mei WANG Jun-juan FANG Bao-xing 《中国农业科学(英文版)》2010,9(6):783-791
To study the relations between DNA methylation and abiotic stress responses in cotton （Gossypium hirsutum L.）,the methylation-sensitive amplified polymorphism （MSAP） method was used to investigate the differences in methylation level and the change of cytosine methylation patterns under salt （NaCl） stress in two different salt-tolerant cotton lines.The results showed that the number of the cytosine methylation of CCGG sites in high salt-tolerant cotton line was less than that in low salt-tolerant line.Under salt stress,extensive cytosine methylation alterations including hypermethylation and demethylation as well as the potential conversion of methylation types occurred in the salt-treated cotton line compared with the corresponding control.Interestingly,salt stress-induced demethylation loci that occurred in high salt- tolerant cotton line were greater than those in low salt-tolerant cotton line,however,salt stress-induced hypermethylation loci in the high salt-tolerant cotton line were less than those in low salttolerant cotton line.It suggested that the demethylation positively contributed to salt tolerance and the hypermethylation had negative effect on salt tolerance in cotton. 相似文献
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不同剂量CpG序列对仔猪的免疫增强效果 总被引：7，自引：0，他引：7
以伪狂犬病毒为模式病毒,按照仔猪体质量分别将100、10、1μg/kg CpG序列同猪伪狂犬疫苗联合免疫接种仔猪,检测仔猪的体液及细胞免疫反应．试验结果表明：不同浓度的CpG序列均能显著提高仔猪的特异性抗体滴度、白细胞介素(IL)-2诱生活性以及淋巴细胞增殖反应,证明CpG序列能显著增强仔猪对常规疫苗的免疫应答能力。 相似文献