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不同基因型小麦对除草剂Basta敏感性研究 总被引：2，自引：0，他引：2
为确定抗除草剂抟基因小麦研究中的筛选压，以4个小麦品种(系)的成熟种子为材料，设置5种除草剂Basta浓度进行抗性鉴定。结果表明，不同基因型小麦对Basta的敏感性差异明显。Basta对小麦发芽成苗无明显影响，对幼苗高度和根长度的影响差异极显著。随着Basta浓度的增加，小麦幼苗生长受到抑制，高度明显降低，黄化率显著增大。Basta浓度为9mg／L的处理适宜，播种后4～5d出现黄化苗，黄化率达60％～70％，且对小麦幼苗的生长影响相对较好。 相似文献
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K. Rajasekaran R. Majumdar C. Sickler Q. Wei J. Cary D. Bhatnagar 《Journal of Crop Improvement》2017,31(4):628-636
Screening of gene manipulation events (transgenic, mutation/genome editing, etc.) is a cost/labor-intensive and time-consuming process in plant science research. While polymerase chain reaction (PCR) is the most commonly used method for screening, the process still requires efficient DNA extraction and subsequent confirmation. However, PCR cannot predict gene expression. To screen a larger number of transgenic plants, it would be ideal to develop a quick and reliable screening procedure. We have applied a Liberty® leaf-painting method (against bar gene under 4x35S promoter) to screen transgenic maize (Zea mays L.) plants and validated the results through PCR and quantitative real-time PCR (qRT-PCR). Liberty leaf painting at 500 mg L?1a.i. was > 95% accurate in identifying transgenic events that agreed with the PCR results. Further investigation of bar gene expression in sensitive lines that were PCR positive shows very low expression of the bar gene. We have provided a simple, and rapid assay to determine the transgene expression potential of maize plants expressing the bar gene. The herbicide can be applied to a fully expanded leaf and evaluated one week after application. Green or partially green leaf blades indicate high or moderately high expression of the bar gene and a total yellowing indicates absence or extremely low expression of the bar gene in the transgenic plants. A small volume of Liberty solution is adequate to test hundreds of maize plants, and the assay is reproducible with a high frequency (> 95%) and also displays good correlation with gene expression in planta. 相似文献
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以二球悬铃木(Platanus acerifolia)的下胚轴和离体叶片为材料,研究了植物遗传转化过程中常用的2种选择剂卡那霉素(Kan)和除草剂(PPT)对其不定芽分化的抑制作用,同时试验了2种选择剂对试管苗生根的影响。结果表明,Kan和PPT对悬铃木下胚轴和离体叶片的不定芽分化及试管苗生根均表现出较强的抑制作用,其中12.5-20.0 mg/L Kan或2.0 mg/L PPT能完全抑制叶片外植体正常的不定芽分化,50 mg/L Kan或1.0 mg/L PPT可完全抑制下胚轴的不定芽分化;Kan和PPT抑制试管苗生根的选择浓度分别为50 mg/L和4.0 mg/L。试验确定了以悬铃木的下胚轴和叶片为转化受体时不同筛选阶段Kan和PPT的选择压力,为其基因工程改良奠定了基础。 相似文献
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以芒(Miscanthus sinensis)胚性愈伤组织为材料,研究了植物遗传转化过程中常用的潮霉素(Hyg)、遗传霉素重硫酸盐(G418)和除草剂(Basta)等3种筛选剂,不同浓度下的不同培养时间对愈伤组织生长、不定芽分化以及再生植株生根的抑制作用。实验结果表明,3种筛选剂均表现出很强的抑制效果。使用75mg/L G418筛选培养20d,50mg/L Hyg筛选20d或20mg/L Basta筛选40d可完全抑制愈伤组织的生长;使用60mg/L G418,40mg/L Hyg或10mg/L Basta筛选培养约20d可完全抑制愈伤组织分化出不定芽;而使用60mg/L G418,50mg/L Hyg或10mg/L Basta筛选培养20d可完全抑制再生植株的生根。3种筛选剂中以Basta对芒植株再生各个阶段的抑制效果最为明显,Hyg的抑制效果次之,G418抑制效果相对不明显。 相似文献
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以常用的筛选剂潮霉素(Hyg)、除草剂Basta和卡那霉素(Kan)对胡萝卜愈伤组织进行敏感性试验,结果表明:胡萝卜愈伤组织对潮霉素和除草剂Basta非常敏感,筛选浓度分别以8mg/L和2mg/L较为合适;卡那霉素合适的筛选浓度应为150mg/L.以带bar基因的质粒载体对胡萝卜愈伤组织进行遗传转化,获得抗性愈伤组织和再生植株. 相似文献
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以2个水稻品种J59和B9363及其转Bar基因株系TR5、TR6为材料,研究了除草剂Basta不同喷施浓度、喷施剂量及喷施时间对转基因水稻秧苗叶绿素含量的影响。结果表明:Basta浓度对转基因和非转基因水稻叶绿素含量影响差异较大,喷施剂量和不同施药时期对其影响次之。在两叶期喷施标准浓度(3.2 g/L)Bas- ta,2个转基因株系TR5和TR6均表现良好的耐药性,在三叶一心期,TR5的耐药性好于TR6。 相似文献
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