为探究长期盐度胁迫条件下红螯光壳螯虾肠道基因表达和代谢通路的变化。实验采用Illumina Hiseq 2 500平台对0、5、10和15这4个盐度下养殖5周的红螯光壳螯虾的肠道组织进行了高通量测序。研究共获76 534个unigenes,通过与NR、NT、KO、Swissprot、PFAM、GO和KOG数据库进行比对,成功注释37 378个unigenes。通过引入FPKM(Fragments Per Kilo bases per Million fragments)来估算基因表达水平,基于NR和Pfam两个数据库的蛋白注释结果,448 362个unigenes在GO数据库得到注释。根据KO功能注释与Pathway的联系,9 483个unigenes在KEGG数据库被注释分类到34条通路途径。通过DEGseq进行基因表达差异分析,以盐度0为对照组,显著差异基因筛选条件设置为Q value<0.05且差异倍数|Fold Change|>2,盐度5、10和15组分别获得差异基因2 733、91和236个,其中盐度5组共有2 068个基因显著上调,665个基因显著下调。将所有差... 相似文献
A trial was conducted in 12 purpose-built, commercial, drainable, earthen ponds to evaluate the effect of fish and plant protein and lipid source on the growth, condition indices, and body composition of marron (Cherax tenuimanus). Juvenile marron (1.3±0.28 S.E. g) at the stocking densities of three per square meter were fed for a period of 1 year with four different formulated isoenergetic practical diets (D1, D2, D3, and D4). Three of the test diets (D1, D2, and D3) were isonitrogenous whereas the fourth test diet (D4) was protein-free. Protein and lipid sources in D1 were from Lupin (Lupinus albus) whereas protein and lipid sources in D2 and D3 were from fish meal. Fish oil (3.5%) was added to D1, D3, and D4 whereas sunflower oil was added to D2 and D4 in order to make them isoenergetic. The four test diets were randomly allocated to three replicate ponds.
The lack of protein in D4 did not significantly influence (P>0.05) the mean final weight and specific growth rate of marron. Survival was low in all ponds (13.82–34.66%) but feeding with D4 resulted in a significantly (P<0.05) higher survival than marron fed with D1 and D2. Feeding a diet containing a combination of fish protein and fish oil (D3) resulted in significantly higher (P<0.05) wet tail muscles-to-body weight ratio than was observed with other diets. Tail muscles protein level of all marron was significantly lower (P<0.05) at the end of the trial than at the beginning. EPA and DHA in hepatopancreas and tail muscles of marron were affected by the four test diets. The inclusion of plant protein in formulated diets had a negative impact on the pond environment due to significantly higher unionised ammonia levels that resulted in lower survival. Juvenile marron fed with a plant protein diet had significantly lower protein levels in their hepatopancreas compared to those fed with diets containing animal protein. Feeding marron with lupin protein source (D1) and plant oil (D2) for 1 year did not alter the lipid content of their hepatopancreas. Four test diets had no influence on the fat content of marron hepatopancreas; however, these test diets significantly reduced the protein content of the tail muscles of marron.
Environmental variables, particularly temperature, nitrogen metabolites, and the natural productivity of the ecosystem, greatly influenced the nutritional requirements of the juvenile marron under culture. 相似文献
Ninety sexually mature Cherax quadricarinatus females were exposed to various combinations of photoperiod and temperature for 2 months during the summer. Females were randomly assigned to either “winter” “semi-winter” or “summer” simulation treatments. In the “winter” treatment, crayfish were exposed to a simulated winter photoperiod (gradual decrease from 14L:10D to 10L:14D, 4 weeks at short day length followed by gradual increase to 14L:10D) and temperature (gradual decrease from 27 to 15 °C, held for 4 weeks, and then gradual increase to 27 °C). In the “semi-winter” treatment, crayfish were exposed to a simulated winter photoperiod and a summer temperature (27–29 °C). In the “summer” treatment, the crayfish were exposed to summer water temperatures and a photoperiod of 14L:10D. Following the 2 months of conditioning, the females were stocked for 7 months in small groups with males under environmental conditions similar to those of the “summer” treatment. All females were individually tagged and molting and spawning events were monitored. Females exposed to “semi-winter” conditioning in the summer, demonstrated a threefold increase in the rate of first spawning during the winter (December–February) compared with the other females. Crayfish breeders can easily implement these findings since shifting the breeding season into the winter only requires shortening of the photoperiod in the summer. The stocking of ponds in the spring with large nursed juveniles that hatched from eggs spawned in the winter, would allow the attainment of market size at the end of the limited growout season in temperate zones. 相似文献
A feed trial was conducted for 12 weeks on juvenile Australian freshwater crayfish (Cherax destructor) (mean weight (SE) 0.82 (0.02)g) maintained on five isoenergetic diets with a protein content of 30%. Diets differed in the primary source of protein used, with meat, snail, soybean, yabby, and zooplankton meals comprising the major protein ingredient, varying from 56–60% of total protein. Mean percentage weight gain per day ranged from 7.57% (yabby meal diet), to 9.42% (snail meal diet). No significant difference occurred in mean weight, percentage weight gain, specific growth rate (%), or survival among diets. A maximum size of 16.44g was attained on the yabby meal diet. Largest mean weight was 8.27g on the snail-based diet. Food conversion ratios were all good, with a minimum value of 0.95 recorded for the snail-based diet. Initial food consumption per day was approximately 5% of mean animal weight, decreasing to around 2.4%, and is collectively described by a power curve. Protein retention ranged from 29.57% in the zooplankton meal diet to 41.15% in the snail-based diet. Carcass composition was influenced by feed type, with the most marked difference occurring in carapace colour. Animals fed the zooplankton-based pellets developed the strongest pigmentation. Recommendations are made for including certain protein-based ingredients in manufactured yabby diets. 相似文献
Cherax albidus (A) and Cherax destructor (D) male juveniles (mean weight 0.95 ± 0.03 g) were reared for 20 weeks on isoenergetic diets containing 150 g kg?1 protein (A 15, D15) or 300 g kg?1 protein (A30, D30). Mean weight, percentage weight gain, and specific growth rate (%) were substantially higher for both species on the 300 g kg?1 protein diet. Mean percentage weight gain ranged from 2.39% day?1 (D15) to 17.59% day?1 (A30). A maximum weight of 33.81 g was attained by C. albidus on the higher protein diet. The most effective utilization of food was observed in C. albidus when fed the higher protein diet (food conversion ratio, 0.79; protein efficiency ratio, 4.21; apparent net protein utilization, 44.64%). Carcass composition was influenced by feed type. The higher protein diet resulted in an increase in carcass protein and ash and a decrease in carcass lipid and energy relative to the low-protein diet (150 g kg?1 protein diet –C. albidus: 37.15% protein, 15.00% lipid, 25.20% ash, 15.55kJ g?1 energy; C. destructor: 38.10% protein, 15.43% lipid, 25.70% ash, 15.65kJ g?1 energy; 300 g kg?1 protein diet –C. albidus: 46.10% protein, 8.71% lipid, 27.36% ash, 14.94kJ g?1 energy; C. destructor: 42.99% protein, 8.56% lipid, 26.44% ash, 14.71kJ g?1 energy). Carcass moisture and calcium were not affected by feed type. The time spent in the intermoult phase of growth was highly dependent on the premoult weight and varied according to diet and to species. A comparison of animals of similar weight (< 8 g) revealed that elevated dietary protein caused a reduction in the intermoult period by 11 days in C. albidus and 7 days in C. destructor. The moult increment, however, was independent of animal weight, and the highest percentage weight increment occurred for C. albidus fed the 300 g kg?1 protein diet (per cent weight increase; A15, 33.1%; A30, 61.3%; D15, 31.2%; D30, 56.5%). Dietary induced morphological changes were also recorded. Animals of a standard carapace length had significantly larger abdomens (both species) and larger claws (C. albidus) when fed the higher protein diet. 相似文献
In the process of exploring ways to intensify crayfish culture, a growout system of individual cages (cells) was designed to determine the effects of gender and cell size on the growth of the red claw crayfish Cherax quadricarinatus. Cells of three different diameters—large (25 cm), medium (20 cm) and small (16 cm)—were used. When crayfish were stocked at a mean weight of approximately 10 g, growth rate of males was significantly higher than that of females. The growth rate of the males in the large cells was 0.31±0.14 g/day, while that of the females was 0.18±0.09 g/day. The size of the cell had significant influence on the weight of males. Male crayfish in the large and medium cells grew better than those in the small cells. When males were stocked at a higher mean weight (about 23 g), their mean weight after 206 days was higher in the large cells (69.28±15.72 g) than in the small cells (58.11±12.66 g), suggesting that the growth of large males was also affected by cell size. Regardless of cell size, male animals of this species grew faster than females under conditions of individual cells. This intensive culture method appears to present a powerful improvement in yields, by as much as two orders of magnitude, in comparison with communal cultures. 相似文献