Decreased transport of orally administered protein into the blood circulation of developing juveniles of Japanese eel Anguilla japonica     

Osamu Nakamura  Yuzuru Suzuki  Katsumi Aida  Hajime Hatta 《Fisheries Science》2001,67(5):863-869

ABSTRACT: To clarify the quantitative changes in the transport of orally intubated protein into the blood circulation as macromolecules in development, immunoglobulin Y (IgY) extracted from chicken eggs was administered orally to juvenile Japanese eel, Anguilla japonica . For the first experiment, which was performed before the commencement of artificial feeding, the oral delivery of 2.0 μg/0.1 g bodyweight of IgY resulted in a rapid increase in plasma IgY to a maximum of 2.30 μg/mL. However, the transport of IgY into the blood decreased significantly in the experiments that followed, which were performed after 12, 25 and 42 days. During this period, bodyweight increased approximately by a factor of eight, and rapid growth of the stomach was observed histologically. Possible contributions for the development of the alimentary canal to the diminishment of intestinal protein assimilation are discussed.  相似文献   

Patterns of silver eel (Anguilla anguilla L.) sex ratio in a catchment     

P. Laffaille  A. Acou  J. Guillouët  B. Mounaix  A. Legault 《Ecology of Freshwater Fish》2006,15(4):583-588

Abstract –  Changes in the numbers and size-class structure of European silver eels, Anguilla anguilla , in the River Frémur (France) were examined over a 9-year period after installation of downstream eel passes. The number of silver eels migrating downstream peaked in 1999, then decreased strongly and steadily after 2000, reaching relatively low levels. At the same time, a gradual shift in the silver eel sex ratio from a dominance of males (size from 270 to 442 mm, age from 3 to 6 years) to females (size from 366 to 1112 mm, age from 4 to 9 years) was recorded. Possible explanations for the escapement patterns observed are environmental sex determination and the installation of eel passes on the main hydraulic engineering structures in 1992 and 1996.  相似文献   

GMP玻璃化冷冻未成熟牛卵母细胞核成熟及冷冻损伤试验     

许卫华  王学琼  王子玉  王锋  张嘉保 《中国兽医学报》2011,31(6)

本试验通过荧光染色的方法建立了未成熟牛卵母细胞在体外培养过程中第1次减数分裂的各个阶段的参考判定图谱;根据这个标准来观察毛细玻璃管(GMP)玻璃化冷冻对卵母细胞核成熟和冷冻损伤的影响。结果表明,从屠宰场废弃的卵巢表面卵泡内抽取的COCs,70%处于生发泡期,12.5%生发泡开始破裂,7.5%已开始浓缩,这说明从屠宰场获得的COCs有较高的异质性;卵母细胞在成熟培养22h时收获排出第一极体的卵母细胞,可得到丰度较高的极体-胞质染色体对称、紧密相邻的成熟卵母细胞;GMP玻璃化冷冻损伤主要有2种表现形式,首先,直接影响膜结构的完整性,包括细胞膜和核膜,这可从退化的细胞比例看出(8～24h,有21.9%～27.2%的细胞处于该阶段),其次,影响CONDENSED向MⅠ期的过渡,这可从处于CONDENSED卵母细胞的比例看出(8～24h,有24.1%～34.3%的细胞处于该阶段)。  相似文献   

Study on The origin of The Cracks And Their Elinination In Expanded Pearlite And Waste Glass Compound Decorative puank     

Zhang Zhiqiang  Lu Jian  Wu Fang  Zou Yicong 《保鲜与加工》1995,(3):60-64

  相似文献   

现代玻璃温室智能气动天窗系统设计与研究   总被引：5，自引：0，他引：5  

司慧萍  苗香雯  崔绍荣  楼华梁 《农机化研究》2004,(1):125-127,130

为满足现代温室对室内环境越来越高的要求，采用快速、安全、可靠、低成本的气动自动化技术设计气动天窗系统，可以实现天窗系统开闭与开度的自动化调控。为此，讨论了气动天窗系统的原理，得出了驱动气缸负载模型以及各级风速下气缸负载特性，探讨了该系统可行性和特点。与传统技术比较，气动系统完全可以取代机械式开天窗，而且结构简单，操作方便，位置控制精度高，天窗开度和通风面积显著增加，增强了利用自然通风调节温室环境的能力。  相似文献   

真空平板玻璃传热理论分析及试验   总被引：3，自引：0，他引：3  

张瑞宏  马承伟  缪宏  张义  孔德军 《农业机械学报》2006,37(12):134-138

利用辐射-导热耦合模式分析真空平板玻璃的传热途径,通过传热量和传热系数的计算模型,建立真空玻璃的传热控制方程,计算出真空玻璃结构沿高度方向的温度分布和热流通量,并用试验验证了理论计算结果正确性。试验结果表明,真空玻璃传热系数与真空压强成正比,真空度决定真空玻璃绝热性能;真空玻璃外面镀低辐射膜隔绝了较多的辐射热交换,其效果比内面镀膜好。  相似文献   

黄鳝排尿器官的解剖学研究     

曾增  刘希彬  吴峻岩  李琦  谢从新  杨春 《华中农业大学学报》2002,21(1):72-76

应用铸型技术等方法研究了黄鳝排尿器官的解剖学结构。结果见到,黄鳝2条中肾管在中肾末端与所谓退化性腺（亦即膀胱）相连,并与该“退化性腺”之间存在着明显的通道关系,而“退化性腺”直接通于尿孔。此外,组织学的研究也支持了解剖学结果,并证实中肾管在“退化性腺”中存在着2种开口类型;单口型和双口型。研究结果为作者先前提出的有关黄鳝“退化性腺”是膀胱的结论提供了直接的形态学证据。  相似文献   

Transient heat transfer characteristics of air-array-jet impingement with different nozzle arrangements on high-temperature flat plate in small jet-to-plate distance     

LI Meixiang  ZHU Keqian  XIE Yu  CAI Tingting  YUAN Ningyi  DING Jianning 《排灌机械工程学报》2020,38(3):277-284

Numerical studies on transient heat transfer characteristics of air-array-jet impingement with a small jet-to-plate distance and a large temperature difference between nozzles and plate were presented.The dimensionless jet-to-plate distance(H/D)was 0.2,and non-dimensional nozzle-to-nozzle spacing(S/D)was 3,4,5 and 6,respectively.It is found that the quenching time is shortened at a constant total mass flow at air jet inlet m·(m·=218.21 kg/h),and the heat transfer uniformity is deterio-rated as S/D increases.However,the adding reversed-flow nozzles can shorten the quenching time of the glass plate considerably with a modest change in the heat transfer uniformity.The results at variable m·are the same as those at a fixed m·.Furthermore,the parity and arrangement of nozzles are also discussed,It is found that an odd number of nozzles is more beneficial for transient heat transfer.Based on these results,an appropriate proposal for ultra-thin glass tempering process is presented.  相似文献   

Fluorescence localization of epidermal cathepsins L and B in the Japanese eel     

F. Aranishi  N. Mano  H. Hirose 《Fish physiology and biochemistry》1998,19(3):205-209

Histochemical localization of proteolytic activities in the dorsal epidermis of Japanese eel was demonstrated by fluorescent microscopy utilizing 4-methoxy-2-naphthylamide (4M$\beta$NA) derivatives as substrates and 5-nitrosalicylaldehyde as a trapping agent. Carbobenzoxy-L-phenylalanyl-L-arginyl-4M$\beta$NA (Cbz-Phe-Arg-4M$\beta$NA) and Cbz-Arg-Arg-4M$\beta$NA were used for direct detection of cathepsins L and B activities, respectively, in fresh frozen sections and unfixed cells of the eel epidermis. The fluorescing areas, where Cbz-Phe-Arg-4M$\beta$NA was hydrolyzed by cathepsin L, were shown in mucus secretory cells and club cells and broadly around skin surface. The fluorescing areas due to Cbz-Arg-Arg-4M$\beta$NA hydrolysis by cathepsin B were localized similarly in these tissues. The fluorescing intensity for both catheptic activities in mucus secretory cells was higher than that in club cells, where small fluorescing granules were distributed. These results indicate that eel cathepsins L and B are stored in epidermal secretory cells at different levels and probably serve as defense factors before or after secretion by these cells. Abbreviations: Cbz – carbobenzoxy; 4M$\beta$NA – 4-methoxy-2-naphthylamide; NSA – 5-nitrosalicylaldehyde.  相似文献   

二株鳗鲡益生菌对欧洲鳗鲡免疫应答的初步研究   总被引：1，自引：0，他引：1  

林煜 《福建水产》2006,(4):44-47

由养殖鳗鲡肠道分离、筛选出的益生菌A40209CDC4和A31009NA,添加鳗鲡饲料,细菌浓度达107cfu·g-1,连续投喂30d,测定试验鳗鲡白细胞吞噬活性、溶菌酶活力和血清抗体滴度.结果显示:与未添加细菌的对照组相比,添加益生菌试验组的白细胞吞噬百分比、吞噬指数、溶菌酶活力和血清抗体滴度无显著变化,说明筛选菌株作为饲料添加剂应用于欧洲鳗鲡未产生特异性刺激.  相似文献