同期排卵处理母牛体温和活动量变化规律及效果分析     

田宏志  陈晓丽  李欣  李茹意  王栋 《畜牧兽医学报》2020,51(7):1619-1627

为探究同期排卵处理母牛体温和活动量变化规律及不同同期排卵技术处理效果，指导同期排卵技术优化。本研究自动监测了18头20月龄左右同期排卵（GnRH-PG-GnRH）处理荷斯坦母牛和17头产后40~60 d预同期排卵（PG-PG-GnRH-PG-GnRH）处理荷斯坦母牛的体温和活动量，应用自动检测系统进行母牛发情监测。结果发现，同期排卵处理母牛发情时阴道温度平均升高（0.43±0.20）℃，持续（12.37±2.73）h；活动量平均升高（18.28±18.61）倍，持续（11.00±1.68）h；排卵时阴道温度平均下降（0.20±0.10）℃，持续（11.00±1.68）h。自动化发情监测显示，同期排卵处理母牛7头发情并排卵；预同期排卵母牛GnRH处理前全部发情排卵。两种同期排卵处理，虽可改变母牛性周期进程，促进母牛性周期同步化，但均难以使母牛性周期完全同步。因此，将同期排卵-定时输精和发情鉴定技术科学结合才能取得更好的繁殖效果。  相似文献   

CIDR PMSG FSH联合处理不同品种绵羊效果的研究     

雷赵民  吴建平  张海容  马志愤  王家贵 《中国草食动物》2006,26(4):28-30

CIDR、PMSG、FSH联合使用对初产供体羊Borderdale(波德代)3♂15♀、Poll Dorset(无角陶赛特)5♂18♀和Ger-man Mutton Merino(德国肉毛兼用美利奴—德美)3♂15♀进行同期发情和超数排卵。同期化发情波德代、无角陶赛特和德美分别为100.00%、88.89%和93.33%,羊只同期化发情时间经历和各时间点分布3个品种各有特点。超数排卵效果品种间存在差异,波德代、无角陶赛特、德美分别为6.60±2.97(枚)、3.63±2.25(枚)、5.50±3.32(枚),波德代极显著地高于无角陶赛特(P<0.01)。超排有效率波德代、无角陶赛特、德美分别为100.00%、87.50%、92.86%。未受精卵波德代极显著地高于德美羊和无角陶赛特羊(P<0.01)。胚胎总数德美羊极显著地高于波德代(P<0.01)。有效胚胎波德代羊、无角陶赛特羊、德美羊分别为2.40±2.31、2.75±2.05、4.07±3.79,德美极显著地高于波德代(P<0.01)。同一方法进行同期化发情,羊只经历时间和各时间点均衡分布能否有效控制是同期发情的焦点问题,是有效胚胎数目差异的主导因素之一。  相似文献   

人绒毛膜促性腺激素对母驴促排卵效果的研究     

人绒毛膜促性腺激素对母驴促排卵效果的研究 《畜牧与饲料科学》2021,42(3):59-65

[目的]研究人绒毛膜促性腺激素（human chorionic gonadotropin,hCG）对母驴卵泡发育、排卵率、受胎率以及血清生殖激素水平的影响。[方法]选择优势卵泡直径在30~35 mm以及大于35 mm的母驴各30头,不同优势卵泡直径的母驴群体分别设置1个500 IU/头hCG处理组（n=10）、1个1 000 IU/头hCG处理组（n=10）、1个不接受hCG处理的对照组（n=10）。采用肌肉注射方法对各组母驴进行hCG处理。每隔24 h进行1次B超检查,观察各组母驴卵泡发育情况,测量卵泡直径;记录各组发生排卵的母驴数量,计算排卵率。对各组母驴进行人工输精,输精后第18天进行孕检,记录各组受胎母驴头数,计算各组受胎率。于hCG处理后0、24、48、72 h分别测定各组母驴血清中雌二醇（estradiol,E₂）和孕酮（progesterone,PROG）水平。[结果]2个群体母驴的卵泡直径随hCG注射剂量的增加而增大;优势卵泡直径大于35 mm的母驴群体中,肌肉注射hCG的2个组在处理后24 h内均出现排卵,而对照组母驴没有排卵;优势卵泡直径不同的2个母驴群体,在hCG处理48 h后排卵母驴数和排卵率与对照组相比均有所提高,其中,hCG处理后72 h,优势卵泡直径大于35 mm的母驴群体中,1 000 IU/头 hCG处理组的排卵率达到100%。2个母驴群体中,接受hCG处理的母驴,受胎率均高于对照,并且随hCG剂量的增加,受胎率有所提高;优势卵泡直径大于35 mm的母驴群体中,1 000 IU/头 hCG处理组的受胎率达到50%。2个母驴群体中,1 000 IU/头处理组在hCG处理后24 h的血清E₂浓度均较0 h时有较大幅度的提升,在0~72 h内血清PROG浓度的总体提升幅度较大。[结论]hCG处理可提高母驴的排卵率、受胎率以及血清中E₂和PROG水平,1 000 IU/头剂量的效果更好。  相似文献   

Preventive effects of CIDR-based protocols on premature ovulation before timed-AI in Ovsynch in cycling beef cows     

Sakase M  Kawate N  Nakagawa C  Fukushima M  Noda M  Takeda K  Ueno S  Inaba T  Kida K  Tamada H  Sawada T 《Veterinary journal (London, England : 1997)》2007,173(3):691-693

Ovsynch is a program developed to synchronize ovulation for timed breeding. In this paper, the authors investigate whether controlled internal drug release (CIDR)-based protocols prevent premature ovulation before timed-artificial insemination (AI) when Ovsynch is started a few days before luteolysis in cycling beef cows. Nine beef cows at 16 days after oestrus were treated with (1) Ovsynch, i.e. gonadotropin releasing hormone (GnRH) analogue on day 0, prostaglandin (PG) F(2alpha) analogue on day 7 and GnRH analogue on day 9 with timed-AI on day 10, (n=3); (2) Ovsynch+CIDR (Ovsynch protocol plus a CIDR for 7 days from day 0, n=3), or (3) oestradiol benzoate (OB)+CIDR+GnRH (OB on day 0 in lieu of the first GnRH treatment, followed by the Ovsynch+CIDR protocol, n=3). In the Ovsynch group (1) plasma progesterone concentrations fell below 0.5 ng/mL earlier (day 5) than in both CIDR-treated groups (2) and (3), where this occurred on day 8. Plasma oestradiol-17beta concentrations peaked on day 8 in the Ovsynch group and on day 9 in both CIDR-treated groups. The dominant follicle ovulated on day 10 in the Ovsynch group and on day 11 in both CIDR-treated groups. Thus, both CIDR-based protocols prevented premature ovulation before timed-AI in Ovsynch when the protocol was started a few days before luteolysis. This reflects the fact that progesterone levels remained high until the beef cattle were treated with PGF(2alpha).  相似文献   

The post-ovulatory rise in progesterone is lower and the persistence of oestrous behaviour longer during the first compared with the second cycle of the breeding season in mares     

John R. Newcombe  Sandra Wilsher  Juan Cuervo-Arango 《Reproduction in domestic animals》2023,58(1):141-145

Mares are seasonally polyoestrous breeders. Therefore, the first ovulation of the season, following winter anoestrus, is the only cycle in which mares ovulate without the presence of an old CL from the previous cycle. The objective of this study was to compare the length of oestrous behaviour, and plasma progesterone concentrations during the early post-ovulatory period between mares after the first and second ovulation of the breeding season. Overall, 38 mares and 167 oestrous periods were used in the study. From those, 11 mares were used during the first and subsequent oestrous period to measure and compare the post-ovulatory rise in progesterone concentration, whereas all the mares were used to compare the length of the post-ovulatory oestrous behaviour between the first and subsequent cycles of the breeding season. The persistence of the post-ovulatory oestrus was longer (p < .001) following the first ovulation of the year (median of 52 h) compared with the subsequent ovulations (median of 36 h for second and later ovulations groups; n = 38 mares). The progesterone concentration at any of the four 8 h-intervals analysed (28, 36, 76 and 84 h post-ovulation) was lower (p < .01) following the first versus the second ovulation of the year. By 36 h post-ovulation the progesterone concentration of mares at the second ovulation of the year had passed the threshold of 2 ng/ml (2.1 ± 0.33 ng/ml), whereas in the first cycle it was 1.2 ± 0.13 ng/ml. In conclusion, mares had lower progesterone concentrations in their peripheral circulation and longer persistence of oestrous behaviour following the first ovulation of the year compared with the second and subsequent ovulatory periods of the breeding season.  相似文献   

He—Ne激光照射对牛超排效果的影响机理   总被引：1，自引：0，他引：1  

王志刚  桑润滋 《河北农业大学学报》1999,22(4):129-132

试验采用Ｈｅ－Ｎｅ激光穴位照射供体母牛，并结合纯化ＦＳＨ超排处理，以研究激光照射对牛超排效果的影响机理。结果表明：①Ｈｅ－Ｎｅ激光照射可提高牛超排效果，增加胚胎数和可用胚胎数，试验组头均胚胎数比对照组多２．２枚，头均可用胚胎数比对照组多１．９枚，差异显著（ｐ〈０．０５）；②Ｈｅ－Ｎｅ激光照射通过神经和体液调节，可显著提高血浆中ＦＳＨ（促卵泡素）水平，而ＦＳＨ水平升高是促使供体母牛卵泡发育，增加胚胎  相似文献   

Periovulatory endocrinology and oocyte maturation in unmated mature blue fox vixens (Alopex lagopus)     

W Farstad  M Mondain-Monval  P Hyttel  A J Smith  D Markeng 《Acta veterinaria Scandinavica》1989,30(3):313-319

Nine of 10 mature blue fox vixens (Alopex lagopus) in spontaneous oestrus ovulated approximately 2 days after the preovulatory increase in luteinizing hormone (LH). Plasma concentrations of follicle-stimulating hormone and progesterone increased simultaneously with the LH peak, whereas oestradiol-17 beta peaked 1 day previously. In the tenth vixen, an LH peak was not observed, and neither visible follicles nor corpora lutea were found in the ovaries 6 days after peak vaginal electrical resistance. Eggs were ovulated as primary oocytes, but oocyte maturation was initiated within the day of ovulation (2 days after the LH peak). Within the next 2 days (3-4 days after the LH peak) the first polar body was extruded, and the cumulus mass was completely dissociated from the zona pellucida. The interval between the preovulatory LH peak and initiation of the final oocyte maturation is thus considerably longer in the blue fox than for example in the cow (48-72 h compared with 9-12 h). This suggests that the relationship between these two events is somewhat different in the blue fox.  相似文献   

哺乳动物精清诱导排卵活性蛋白质研究     

LI Wan-kun 《家畜生态》2007,(5)

在动物的精清里包含一些与动物生殖生理活动密切相关的活性蛋白和多肽。驼科动物的双峰驼、美洲驼、羊驼和澳洲考拉等哺乳动物精清中含有类似促性腺激素释放激素(GnRH)作用的活性蛋白质,诱导排卵因子(ovulation inducing factor,OIF),它能引发外周血液中促黄体素(LH)峰浓度的出现和诱发母畜排卵。OIF被认为是一种新的生物活性蛋白质或潜在的新型激素。甚至报道在牛等自发排卵动物以及人精清中也存在类似的活性蛋白。这类因子的发现对于哺乳动物生殖生理及繁殖的深入研究具有重要的意义,有助于动物克服繁殖障碍及繁殖疾病的治疗等。  相似文献   

哺乳动物精清诱导排卵活性蛋白质研究     

李万坤  李辉  赵兴绪  才学鹏 《家畜生态学报》2007,28(5):1-5

在动物的精清里包含一些与动物生殖生理活动密切相关的活性蛋白和多肽.驼科动物的双峰驼、美洲驼、羊驼和澳洲考拉等哺乳动物精清中含有类似促性腺激素释放激素(GnRH)作用的活性蛋白质,诱导排卵因子(ovulation inducing factor,OIF),它能引发外周血液中促黄体素(LH)峰浓度的出现和诱发母畜排卵.OIF被认为是一种新的生物活性蛋白质或潜在的新型激素.甚至报道在牛等自发排卵动物以及人精清中也存在类似的活性蛋白.这类因子的发现对于哺乳动物生殖生理及繁殖的深入研究具有重要的意义,有助于动物克服繁殖障碍及繁殖疾病的治疗等.  相似文献   

双峰驼精清及其活性组分凝集素寡糖的初步研究   总被引：5，自引：0，他引：5  

潘光武  孙册 《中国兽医学报》1998,18(4):355-358

应用植物血凝素与寡糖结构相同或相似的糖复合物专一凝集的原理与方法,结合小鼠排卵试验,间接观察了公驼精清及其经ＤＥ－５２离子交换层析和高效液相层析提取分离的活性组分的蛋白组成。结果表明,公驼精清内糖蛋白含有２种以上的寡糖,其内的活性组分及诱导排卵因子与所试植物血凝素不发生反应  相似文献