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琴鱼的核型、C带和银染分析   总被引:1,自引:0,他引:1  
张德华 《淡水渔业》2007,37(2):16-19
采用秋水仙素-低渗-空气干燥法和银染,以肾细胞为材料,对琴鱼(Ctenogobius giurinus)染色体的核型、C带和银染(Ag-NORs)进行了研究。结果表明:(1)琴鱼2n=44,核型组成为2n=8m+12sm+24t,NF=64,没有异型性染色体分化;(2)所有染色体着丝粒为阳性C带,而且第1对染色体短臂也呈C带阳性,其着色强度与该对染色体上的着丝粒C带相同;(3)Ag-NORs数目在不同细胞中表现出多态性,数目为1~2个,出现1个Ag-NORs的频率最低(9.8%),出现2个Ag-NORs的频率最高(78%),Ag-NORs主要出现在m1对和sm3对同源染色体上。  相似文献   
2.
布氏石斑鱼的染色体核型、银染和C-带   总被引:1,自引:0,他引:1  
为了补充布氏石斑鱼细胞遗传学数据, 并为石斑鱼染色体进化研究以及石斑鱼遗传育种等工作奠定基础, 实验利用Giemsa染色、C-带和Ag-NORs方法对布氏石斑鱼的染色体核型以及异染色质和具有转录活性的核仁组织区在染色体上的分布位置和数量进行了研究。结果表明,布氏石斑鱼二倍体染色体数目为48, 其染色体组由48条端部着丝粒染色体组成, 绝大多数染色体的着丝粒位置呈异染色质带, 仅在第24对染色体靠近着丝粒的位置检测到银染带, 以上结果均表明, 布氏石斑鱼属于较为原始的类群。  相似文献   
3.
河南紫斑牡丹的细胞学研究   总被引:3,自引:0,他引:3  
本文研究了河南紫斑牡丹的核型、Ag-NORs和Giemsa C带带型.核型为2n=2x=10=6m+2sm+2st;C带所显示的端(T)带和Ag-NORs的数目和位置相同.作者认为,紫斑牡丹染色体的T带,实际上应为N带(NOR带);常规染色显示的所谓随体,实际上是端部NORs.  相似文献   
4.
B. Kjaer    V. Haahr  J. Jensen 《Plant Breeding》1991,106(4):261-274
Associations of 23 quantitative traits and 10 genetic marker characters were examined in 63 chromosome-doubled lines (DH-lines) derived from the F1- generation of a cross between an old and a modern spring barley variety. One fourth of the marker × trait combinations showed significant associations. More than two thirds of these associations were to earliness (heading date). Earliness was found to be controlled by two loci: the previously known eak locus and a new locus designated Ea. It is concluded that the associations of quantitative traits with the earliness loci were caused by pleiotropy. Associations were found between two absolutely linked C-bands on chromosome 3 and QTLs (quantitative trait loci) for lodging, straw diameter, and length of top internode of the straw. The three loci on chromosome 5, eak and the two linked powdery mildew loci, Mla9 and Mlk, were associated with a possible QTL for magnesium concentration in grain. Association to the C-band on chromosome 6 suggests QTLs for TGW (thousand grain weight), straw diameter and magnesium concentration in grain. Locus Estl on chromosome 3 was not associated with any of the quantitative traits.  相似文献   
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