| 鸡毒支原体烯醇化酶单克隆抗体研制及黏附阻断 |
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| 引用本文： | 陈鸿军,沈欣悦,陈丹清,于圣青,丁铲.鸡毒支原体烯醇化酶单克隆抗体研制及黏附阻断[J].中国兽医学报,2012,32(6):862-865,870. |
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| 作者姓名： | 陈鸿军 沈欣悦 陈丹清 于圣青 丁铲 |
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| 作者单位： | 1. 中国农业科学院上海兽医研究所,上海,200241
2. 中国农业科学院上海兽医研究所,上海200241／中国农业科学院家禽研究所,江苏扬州225003 |
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| 基金项目： | 国家自然科学基金资助项目(30871883、31001077);上海市科技兴农重点攻关项目(2007-11-2);中央科研院所基础业务经费资助项目(2008JB12) |
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| 摘 要： | 通过对鸡毒支原体烯醇化酶的克隆和原核表达，利用表达产物免疫小鼠，制备单克隆抗体，以超声波裂解的MG全菌作为筛选抗原，结果获得了6株特异性单抗，分别命名为：1A4、2D7、2E4、2F4、384和3D8。经间接ELISA、免疫荧光试验及Western blot检测发现：1A4具有荧光效价和高滴度的ELISA效价，但无免疫印迹效价；而其他5株单抗，免疫印迹呈阳性，但无表面染色的活细胞免疫荧光。在所有6株单抗中，仅1A4单抗有MG细胞黏附抑制作用，可导致MG的黏附率降低34．40％，而且1A4与其他5种单抗混合后，则黏附率可下降57．69％。这些抗体的制备将为烯醇化酶生物学活性研究奠定基础。
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| 关 键 词： | 鸡毒支原体 烯醇化酶 单克隆抗体 |
Preparation and adherence inhibition by monoclonal antibodies to enolase of Mycoplasma gallisepticum |
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| CHEN Hong-jun,SHEN Xin-yue,CHEN Dan-qing,YU Sheng-qing,DING Chan.Preparation and adherence inhibition by monoclonal antibodies to enolase of Mycoplasma gallisepticum[J].Chinese Journal of Veterinary Science,2012,32(6):862-865,870. |
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| Authors: | CHEN Hong-jun SHEN Xin-yue CHEN Dan-qing YU Sheng-qing DING Chan |
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| Institution: | 1(1.Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;2.Poultry Institute,CAAS,Yangzhou,Jiangsu 225003,China) |
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| Abstract: | To prepare the monoclonal antibodies against MG enolase,we cloned the enolase of Mycoplasma gallisepticum,and immunized the mice with the production of the prokaryotic expression,and used the supersonic lytic MG as screening antigen.At last,6 specific monoclonal antibodies were obtained,named 1A4,2D7,2E4,2F4,3B4 and 3D8.Screening by indirect ELISA,immunofluorescence assy(IFA),and Western blot analysis,1A4 had fluorescence and high ELISA titer,while it didn’t have immunoblotting titer.However,other 5 mAbs had positive immunoblotting titer,while the surface stained cells had no positive results by IFA.The obtained mAbs had a satisfactory immunoreactivity.The preparation of these antibodies established the foundation of the further research of the biological functions of the MG enolase. |
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| Keywords: | Mycoplasma gallisepticum enolase monoclonal antibodies |
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