| 二倍体和四倍体泥鳅线粒体ND-5/6基因多态性的PCR-RFLP分析 |
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| 引用本文： | 郑鹏飞,张桂蓉,魏开建,邹桂伟,王卫民,周玲玲,冉伟.二倍体和四倍体泥鳅线粒体ND-5/6基因多态性的PCR-RFLP分析[J].水利渔业,2011,32(1). |
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| 作者姓名： | 郑鹏飞 张桂蓉 魏开建 邹桂伟 王卫民 周玲玲 冉伟 |
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| 作者单位： | 1. 华中农业大学水产学院／农业部淡水生物多样性保护与利用重点开放实验室／农业动物遗传育种与繁殖教育部重点实验室,湖北武汉,430070
2. 中国水产科学研究院长江水产研究所／农业部淡水生物多样性保护与利用重点开放实验室,湖北荆州,434000 |
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| 基金项目： | 国家科技基础条件平台专项(2006DKA30470-002); 农业部淡水生物多样性保护与利用重点开放实验室开放课题(LFB-CU0708)资助 |
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| 摘 要： | 采用PCR-RFLP分析方法,对洞庭湖、武汉两地的二倍体和四倍体泥鳅线粒体DNAND-5/6基因多态性及4个群体遗传变异和遗传关系进行了研究。结果表明,120尾泥鳅mtDNAND-5/6扩增片段长度均为2.2kb;从13种限制性内切核酸酶中筛选出6种多态性内切酶(HaeⅢ、DraⅠ、RsaⅠ、TaqⅠ、HinfⅠ、MspⅠ),对扩增产物进行酶切,共检测到66种单倍型。泥鳅群体内单倍型多样性和核苷酸多样性分别为0.7679～0.9385和0.01041～0.03212;其中,洞庭湖二倍体核苷酸多样性最高(0.03212),武汉二倍体其次(0.02452),二倍体群体内核苷酸多样性高于四倍体。群体间的核苷酸多样性(π)大小为0.02588～0.04144,平均值为(0.031737±0.000005)。群体间的核苷酸歧化距离(δ)大小为0.00462～0.01617,平均值为(0.010659±0.000003);其中,武汉二倍体和四倍体之间的核苷酸歧化距离最大(0.01617),武汉四倍体和洞庭湖二倍体之间的核苷酸歧化距离最小(0.00462)。MonteCarlo模拟x2检验表明,4个群体间的单倍型频率存在极...
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| 关 键 词： | 泥鳅 二倍体 四倍体 RFLP mtDNA ND-5/6 |
PCR-RFLP Analysis on Mitochondrial ND-5/6 Gene Polymorphisms of Natural Diploid and Tetraploid Loach(Misgurnus anguillicaudatus) |
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| ZHENG Peng-fei,ZHANG Gui-rong,WEI Kai-jian,ZOU Gui-wei,WANG Wei-min,ZHOU Ling-ling,RAN Wei.PCR-RFLP Analysis on Mitochondrial ND-5/6 Gene Polymorphisms of Natural Diploid and Tetraploid Loach(Misgurnus anguillicaudatus)[J].Reservoir Fisheries,2011,32(1). |
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| Authors: | ZHENG Peng-fei ZHANG Gui-rong WEI Kai-jian ZOU Gui-wei WANG Wei-min ZHOU Ling-ling RAN Wei |
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| Institution: | ZHENG Peng-fei1,ZHANG Gui-rong1,WEI Kai-jian1,ZOU Gui-wei2,WANG Wei-min1,ZHOU Ling-ling1,RAN Wei1(1.College of Fisheries,Key Laboratory of Freshwater Biodiversity Conservation and Utilization of Ministry of Agriculture,Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education,Huazhong Agricultural University,Wuhan 430070,China,2.Key Laboratory of Freshwater Biodiversity Conservation and Utilization of Ministry of Agriculture,Yangtze River Fisheries Research Institute,... |
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| Abstract: | Polymerase Chain Reaction-Restriction Fragment Length Polymorphism（PCR-RFLP）analysis was used to investigate mitochondrial ND-5/6 gene polymorphisms,genetic variations and relationships of four natural diploid and tetraploid loach（Misgurnus anguillicaudat |
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| Keywords: | Misgurnus anguillicaudatus diploid Tetraploid RFLP mtDNA ND-5/6 |
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