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1.
为探究不同饲料能量源及其水平对凡纳滨对虾(Litopenaeusvannamei)生长性能的影响,以饲料蛋白质含量为37%,蛋白能量比为20.23 mg/kJ的饲料作为对照组饲料(C),在此基础上分别通过提高饲料碳水化合物、脂肪含量调节饲料能量水平,制作饲料蛋白能量比分别为19.51 mg/kJ (中碳水化合物组, MC)、18.85 mg/kJ (高碳水化合物组, HC)、19.45 mg/kJ (中脂肪组, ML)和18.54 mg/kJ (高脂肪组, HL)的4组实验饲料,在淡水养殖条件下投喂初始体重为(0.6±0.02)g的凡纳滨对虾幼虾56d。结果表明,与对照组相比,提升饲料脂肪水平能显著提高凡纳滨对虾的生长性能和蛋白质沉积率(P<0.05),HL组凡纳滨对虾获得最大特定生长率及蛋白质沉积率;增加饲料碳水化合物水平对凡纳滨对虾的生长性能及蛋白质沉积率无显著影响(P>0.05)。与对照组相比, ML组、MC组、HC组对虾肌肉粗蛋白含量显著提高(P<0.05),HL组对虾肌肉粗蛋白含量较对照组有所提高,但差异不显著(P>0.05),对虾肌肉总脂肪含量则随着... 相似文献
2.
植物乳酸杆菌对凡纳滨对虾生长、消化酶活性和肠道组织形态的影响 总被引：1，自引：0，他引：1
将体质量(8.10±0.13)g的凡纳滨对虾放养在室内500L玻璃纤维水桶中,以不同剂量(0、0.5%、1.0%和2.0%)的植物乳酸杆菌菌液(密度109 cfu/mL)拌料投喂,观察凡纳滨对虾的生长、消化酶活性和肠道组织结构的变化。结果显示,投喂15d后,0.5%和1%菌液组对虾的平均体质量、质量增加率和特定生长率均显著高于对照组(P0.05),饲料系数显著降低(P0.05);0.5%菌液组对虾肝胰腺消化酶和肠道消化酶活性显著增强(P0.05),对虾肠上皮细胞高度显著增加(P0.05)。研究结果表明,投喂0.5%植物乳酸杆菌可显著提高凡纳滨对虾消化酶活性和肠上皮细胞高度,促进对虾生长。 相似文献
3.
试验研究了不同盐度下（3和22）,以鱼粉和大豆浓缩蛋白为蛋白源,配制6种不同动植物蛋白比的饲料对凡纳滨对虾生长、成活和肝胰腺可溶性蛋白质含量的影响,饲养试验为期40 d。结果显示：（1）饲料动植物蛋白比可显著影响凡纳滨对虾增重率、成活率、肝体指数、肥满度和肝胰腺中可溶性蛋白质含量。增重率随饲料动植物蛋白比升高而升高,但当饲料中动植物蛋白比升至29∶8时,增重率不再明显升高,其它指标均先随饲料动植物蛋白比升高至一定程度,而后则稍有下降;（2）盐度22组对虾的增重率、成活率和肥满度显著高于盐度3组对虾,肝体指数却显著低于盐度3组,不同的盐度对凡纳滨对虾肝胰腺可溶性蛋白含量的影响不显著;（3）双因素方差分析结果显示,盐度和饲料动植物蛋白比对凡纳滨对虾增重率、成活率和肝体指数存在显著交互作用,最大值分别出现在盐度22下全动物蛋白饲料组、盐度22下全动物蛋白和动植物蛋白为29∶8的饲料组、盐度3下饲料动植物蛋白比为14∶23的饲料组中;（4）Broken-Line分析表明,3‰盐度下凡纳滨对虾最适饲料蛋白比为29.12∶7.79~30.29∶6.71,盐度22时为26.05∶10.95~29.03∶7.44。结果提示,饲料中氨基酸的组成和含量会随配方中动植物蛋白配比而改变,且不同盐度下凡纳滨对虾对饲料中动植物蛋白比的要求有所不同,但配饵中适当的动植物蛋白比可以满足虾对各种氨基酸的适宜需求。因此,在养殖过程中,需结合实际的养殖环境和饲料蛋白源种类,来设计适宜的实用饲料配方,这样才能达到降低生产成本,提高经济效益的目的。 相似文献
4.
饲料中添加核苷酸对凡纳滨对虾幼虾生长、肠道形态及抗氧化酶活力的影响 总被引：2，自引：1，他引：1
本实验旨在研究外源核苷酸混合物对凡纳滨对虾(Litopenaeus vannamei)幼虾生长性能、体组成、中肠肠道形态和抗氧化酶活力的影响。选取960尾初始体质量为(1.01±0.02)g的凡纳滨对虾,随机分为8组,分别投喂基础饲料和添加5种核苷酸混合物(5′-腺苷酸∶5′-胞苷酸∶5′-尿苷酸二钠∶5′-肌苷酸二钠∶5′-鸟苷酸二钠=1∶1∶1∶1∶1,mix-NT)的实验饲料,各实验组添加量分别为0.1、0.2、0.4、0.6、0.8、1.0和1.2 g/kg饲料,养殖期为7周。结果显示,饲料中添加5种核苷酸混合物对凡纳滨对虾的特定生长率(SGR)和饲料系数(FCR)影响不显著(P>0.05)。外源核苷酸显著影响凡纳滨对虾全虾水分含量(P<0.05),但对全虾粗蛋白、粗脂肪和灰分含量影响不显著(P>0.05)。肝胰指数(HSI)随饲料中核苷酸添加量的增加而显著升高(P<0.05),在0.6 g/kg组达到最高。0.4 g/kg组的肝胰腺谷草转氨酶(GOT)和谷丙转氨酶(GPT)活力及尿酸(UA)含量最低,但与对照组相比差异均不显著(P>0.05)。中肠肠壁厚度和肠绒毛高度均随着核苷酸添加量的增加呈先升高... 相似文献
5.
在室内养殖条件下,进行单因素随机设计动物试验,即5种饲料蛋白质水平（31%、35%、39%、43%、47%;以A～E组表示）处理,投喂平均体质量0.2 g的凡纳滨幼虾,养殖60 d。研究结果表明,蛋白质营养对凡纳滨幼虾生长、免疫与抗氧化指标和消化率特征影响显著。随着饲料蛋白水平的升高,特定生长率显著增加,饲料系数下降,E组两项指标最好,分别为5.52和1.96。高蛋白质含量饲料有利于提高对虾免疫指标水平,血淋巴中血细胞浓度、T-AOC活力、POD活力、溶菌酶活力、总蛋白含量,随着蛋白质水平提高显著增加,各项指标E组最高,比A组分别显著提高133.7%、21%、16.8%、20.8%、19.7%(P<0.05);而SOD活力C组最高,与A组差异显著。凡纳滨幼虾对蛋白质的消化率随饲料蛋白质含量增加显著升高(P<0.05),E组比A组提高8.2%。通过饲料蛋白质水平与特定生长的回归分析,获得凡纳滨对虾幼虾最大生长饲料蛋白质需要量为46.5％。 相似文献
6.
盐度和营养对凡纳滨对虾蜕壳和生长的影响 总被引：3，自引：0，他引：3
采用实验生态学方法以初始体质量为(2.01±0.02) g的凡纳滨对虾为研究对象,投喂不同蛋白水平饲料,实验周期30 d,研究了盐度和饲料蛋白水平对凡纳滨对虾蜕壳和生长的影响。实验采用5×3析因设计,盐度梯度设置为6、12、18、24、30五个水平,饲料蛋白水平梯度设置为30%、36%、42%三个水平。结果表明:(1) 对虾蜕壳相对增重率呈现出随盐度上升而降低的变化趋势,以盐度为6时蜕壳相对增重率为最高。盐度和饲料蛋白水平及其交互作用对实验对虾蜕壳相对增重率影响差异显著(P＜0.05);对虾的特定生长率随盐度升高而上升,盐度和饲料蛋白水平对特定生长率影响差异显著(P＜0.05),其交互作用对特定生长率影响差异不显著。(2) 实验对虾的蜕壳频率,在低盐度水平下随盐度的增加而显著增加(P＜0.05),盐度18时蜕壳频率达到最高,之后随盐度的升高蜕壳频率下降,差异不显著。各盐度水平下,以中等蛋白质水平饲料组(36%)对虾蜕壳频率较高。方差分析表明,盐度和饲料蛋白水平对蜕壳频率影响差异显著(P＜0.05),其交互作用对蜕壳频率影响差异不显著。(3) 对虾蜕壳间期随盐度升高呈先延长后缩短的变化趋势。盐度对对虾蜕壳间期影响差异显著(P＜0.05),饲料蛋白水平单因子以及它和盐度的交互作用对对虾蜕壳间期影响不显著。 相似文献
7.
8.
为了探讨低盐度(0.6～0.8 g/L)条件下初始体质量为(0.38±0.004)g的凡纳滨对虾(Litopenaeus vannamei)幼虾的亮氨酸需求量,以鱼粉、花生麸和L-晶体氨基酸为蛋白源配制含粗蛋白400 g/kg的6种等氮饲料,标记为L7～L12组,各组亮氨酸水平分别为15.95、17.95、19.95、21.95、23.95和25.95 g/kg(饲料干物质).每组3个重复,每个重复30尾虾,进行56 d生长实验.结果表明,凡纳滨对虾幼虾的增重率随饲料亮氨酸水平的升高而升高,当亮氨酸水平达到23.95 g/kg(L11组)时,增重率达到最高值(1143.11±36.40)％,显著高于L7组(P＜0.05);亮氨酸水平继续提高,增重率变化不显著(P＞0.05).幼虾的蛋白质效率、全虾体蛋白沉积率和血淋巴总蛋白随着饲料亮氨酸水平的升高呈现升高趋势,最高值出现在L11组,并显著高于L7组(P＜0.05).各实验组饵料系数、血淋巴谷丙转氨酶、谷草转氨酶活性和尿素氮含量则呈现降低的趋势,最低值出现在L11组,且显著低于L7组(P＜0.05).以增重率为指标,根据折线模型可知,低盐度条件下凡纳滨对虾幼虾的亮氨酸最适需要量为24.80 g/kg饲料,即61.99 g/kg饲料蛋白. 相似文献
9.
以初始体重为5.27±0.20g的凡纳滨对虾为研究对象,分别在饲料中添加0、0.5%、1.0%和2%的盐,配制成4种实验饲料,在盐度为1.5和30的水体中喂养50d,探讨饲料中添加盐对凡纳滨对虾生长性能、虾体成分和碱性磷酸酶活性及风味的影响.结果表明,在盐度为1.5时,饲料中添加盐的各组饲料其特殊增长率(SGR)比对照组均有显著提高(P<0.05);饲料中添加盐组的饲料系数(FCR)均比对照组低(P<0.05),饲料中盐的添加量为2.0%时,饲料系数(FCR)达到最低(P<0.05);饲料中添加盐有助于提高对虾的成活率(P<0.05);随着饲料中盐添加量的增加,对虾体蛋白质含量有增加的趋势,而虾体脂肪含量和水分有下降的趋势;随着饲料中盐添加量的增加,凡纳滨对虾肌肉游离氨基酸中必需氨基酸、非必需氨基酸及游离氨基酸总量有增加趋势,甘氨酸、丙氨酸和谷氨酸等呈鲜味氨基酸都大幅度增加;对虾的色泽、嗅觉、味觉及组织等多项指标的感官评价显示,饲料中添加盐2.0%组的综合感官指标优于对照组及其他实验组;饲料中添加盐可显著提高凡纳滨对虾肝胰脏碱性磷酸酶活性,而且随着饲料中盐的添加量的增加,其酶的活性显著上升(P<0.05).当盐度为30,饲料中添加盐显著降低特殊增长率(SGR)(P<0.05),而且随着盐度的增加,其影响加重;饲料中添加盐显著增加对虾的饲料系数(FCR)(P<0.05). 相似文献
10.
硒源对凡纳滨对虾生长、体组成和抗氧化能力的影响 总被引：1，自引：0，他引：1
水温(29.0±1.3)℃,将初始体质量(0.41±0.01)g的凡纳滨对虾随机分为4组,每组3个重复,饲养在室内循环水养殖系统中,投喂基础饲料(对照组)及在此饲料中分别添加硒含量相等(0.30mg/kg)的亚硒酸钠、酵母硒和蛋氨酸硒的4种饲料,养殖56d,比较了不同硒源对凡纳滨对虾生长、体组成和抗氧化能力的影响。试验结果表明,蛋氨酸硒组对虾的生长性能显著高于对照组和亚硒酸钠组(P0.05)。外源硒可显著影响对虾体蛋白和脂肪含量(P0.05),蛋氨酸硒组对虾机体营养成分显著优于其他组(P0.05)。添加外源硒组对虾的血清总抗氧化能力和谷胱甘肽过氧化物酶活性显著高于对照组(P0.05)。蛋氨酸硒组对虾血清丙二醛含量显著低于其他组(P0.05)。由此得出,饲料添加0.3mg/kg的硒能在一定程度上提高凡纳滨对虾的生长和抗氧化能力,蛋氨酸硒优于酵母硒和亚硒酸钠。 相似文献
11.
不同比例复合益生菌对凡纳滨对虾生长、免疫及抗氨氮能力的影响 总被引：2，自引：0，他引：2
对从凡纳滨对虾(Litopenaeus vannamei)肠道中分离出的鲍鱼希瓦氏菌(Shewanella haliotis)、蜡样芽孢杆菌(Bacillus cereus)和双壳气单胞菌(Aeromonas bivalvium)3株有益菌株,利用正交设计得到9种复合比例,通过饲料中添加上述9种比例混合的菌体(菌数总量为109 cfu/g)饲喂凡纳滨对虾,经过28 d养殖实验,评价其对凡纳滨对虾生长、免疫指标的影响。随后,利用氯化铵调节水体氨氮浓度至26.67 mg/L,经过16 d的氨氮毒性实验,研究不同比例复合益生菌对凡纳滨对虾抗氨氮能力的影响。研究结果表明,9个复合益生菌实验组的增重率和特定生长率均显著高于对照组(P0.05),并且以3株菌菌数6︰1︰3比例效果较好;不同配比的复合益生菌能够显著提高酸性磷酸酶(ACP)、碱性磷酸酶(ALP)、总超氧化物歧化酶(T-SOD)活力(P0.05),并表现出了不同的影响效果,其中,3株菌菌数(菌数总量为109 cfu/g,下同)2︰3︰3、4︰2︰3及6︰1︰3比例对ACP活力具有显著促进作用(P0.05),3株菌菌数4:2︰3和6︰1︰3比例对ALP活力具有显著促进作用(P0.05);3株菌菌数2︰1︰1比例对T-SOD活力具有显著促进作用(P0.05);各比例的复合菌对溶菌酶活力的影响不显著(P0.05);氨氮浓度26.67 mg/L条件下,不同比例复合菌组对虾累计存活率显著高于对照组(P0.05),其中以3株菌菌数4︰3︰1和6︰3︰2比例组累计存活率较高,即抗氨氮效果较好。 相似文献
12.
T. Citarasu K. Venkatramalingam M. Micheal Babu R. Raja Jeya Sekar M. Petermarian 《Aquaculture International》2003,11(6):581-595
The indiscriminate use of antibiotics and chemicals in shrimp hatcheries has led to biomagnification and that in turn could
lead to rejection of a whole consignment. The application of the bioencapsulation technique as a tool for curative treatment
in shrimp larvae was investigated. Herbs having antibacterial properties such as Solanum trilobatum, Andrographis paniculata and Psoralea corylifolia (methanolic extracts) were bioencapsulated in Artemia and fed to Penaeus monodon post larvae PL 1–25. The post larvae were reared in a medium inoculated with pathogenic bacteria such as Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi and Vibrio sp. Post larvae reared in the non-inoculated water and fed with non-enriched Artemia exhibited 90% survival, highest specific growth rate (12.43%) and reduced bacterial load. P. monodon reared in the bacterial inoculated water and fed with the non-enriched Artemia exhibited the lowest survival (10–30%), specific growth rate (8.42–9.1%) and increased bacterial load (2.86 × 103 to 3.76 × 105 cfu/g). The methanolic extracts of the herbs helped to increase survival and specific growth rate and reduced bacterial load
in the P. monodon culture system. Among the three herbal extracts, P. corylifolia enriched Artemia fed post larvae showed the tendency to higher survival (>50%), growth rate (11.5 averaged) and low bacterial load (1.12 × 105 cfu/g).
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
13.
Using mark-recapture methods, the movements of the fluvial form of masu salmon (Oncorhynchus masou masou) in a mountain stream on the island of Kyushu, Japan, were studied. Most (78%) of the masu salmon were recaptured in the
pool in which they had been originally caught and tagged. Of those that moved between pools, the proportion of individuals
that moved during the breeding period was not significantly higher than the proportion that moved during the non-breeding
period. However, during the breeding period, a higher proportion of larger salmon moved than did smaller fish. The proportion
of mobile large males during breeding period was higher than that for small males. Also, it was found that a few individuals
showed long-range movement in the autumn. As a long-term movement, 78 individual fish (65%) that were recaptured more than
three times showed high sedentary tendencies. Sixteen individual mobile fish (13%) moved and returned to the original pool.
Fluvial form of masu salmon in Kyushu show a high sedentary nature; however, large mature males seem to actively move in search
of female during breeding period. 相似文献
14.
S. Mortensen T. Meeren A. Fosshagen I. Hernar L. Harkestad L. Torkildsen Ø. Bergh 《Aquaculture International》2000,8(2-3):267-271
Between 1995 and 1997, a traditional Norwegian oyster poll with an attachednursery was used for the production of scallop (Pecten maximus) spat.During summer 1997, four batches of 2 mm scallop spat were placed in thenursery. In June, hydroids (Obelia) caused problems with fouling andaccumulation of sediment in the nursery. Thereafter, the scallop spat wereheavily infested with tube dwelling polychaets (Polydora sp.). Thepolychaets were both introduced with poll water and reproduced inside thenursery. Infested spat suffered high mortalities. Although the spat could have been affected by the hydroids, as well as shortage of food, Polydorainfestations were considered the main cause of the mortalities. Dead andinfested spat were continuously sorted out and discarded, but the infestationproblems persisted. As a result, the scallop spat did not represent anycommercial value for the company, and in September, the nursery wasemptied. In total, approximately one million spat – representing onethird of Norway's intensive scallop spat production in 1997, was lost. 相似文献
15.
OAA, the potent anti-HIV protein from Oscillatoria agardhii NIES-204 belongs to a new lectin family, shows strict binding specificity for high-mannose N-glycans, and has an extremely high association constant in the picomolar range for recombinant gp120, an envelope protein
of HIV. In this study we have cloned the gene encoding OAA from the genomic DNA of the cyanobacterium, and efficiently expressed
the recombinant lectin (rOAA) in Escherichia coli. The rOAA expressed as a His-tagged fusion protein was recovered in a soluble form and purified in high yield (48 mg/1 l-culture)
by metal chelate chromatography. The fusion protein was cleaved with factor Xa, and the resulting rOAA was isolated in a final
yield of 14.8 mg/1 l-culture by reversed-phase HPLC. Both the N-terminal sequence and the molecular mass of rOAA were found
to be identical with those of OAA. The rOAA was fully functional with the same properties as OAA, as evidenced by hemagglutination
activity, hapten-inhibition test, and binding specificity for high-mannose-type N-glycans. This rOAA should be applicable as a specific probe for high-mannose N-glycans and should contribute to elucidation of the molecular basis of its strict carbohydrate-binding specificity and potent
anti-HIV activity. 相似文献
16.
杜仲对草鱼生长、肌肉品质和胶原蛋白基因表达的影响 总被引：1，自引：1，他引：0
为研究杜仲对草鱼生长性能、肌肉品质及胶原蛋白基因COL1A1和COL1A2表达的影响,实验采用初始体质量为(215.0±0.4)g的草鱼120尾,随机分为2处理组(每组3重复,每重复20尾鱼),分别饲喂基础饲料(对照组)和添加2%杜仲的实验饲料(杜仲组),养殖时间为8周。结果显示,与对照组相比,添加2%杜仲对草鱼生长性能无显著影响,但能显著增加肌肉、皮肤和肝脏胶原蛋白水平,增加肌肉总必需氨基酸(TEAA)、总氨基酸(TAA)水平。2%杜仲可显著降低草鱼肌肉的冷冻失水率、离心失水率,但对肌纤维密度和肌纤维直径无显著影响。在胶原蛋白基因表达方面,2%杜仲显著增加了第4周、8周时草鱼的肌肉、皮肤和第8周时的肝脏组织COL1A1、COL1A2基因m RNA表达量。研究表明,饲料中添加2%杜仲可改善大规格草鱼的肌肉品质。 相似文献
17.
Satisfying nutrient requirement of corals is still a major constraint for maintaining corals in marine aquariums. Corals are
polytrophic in nature. Heterotrophic feeding on zooplankton is one of the corals’ strategies to overcome nutrient deficiency.
Artemia salina nauplii are commonly used as biocarriers for many fish larvae in aquaculture and can also serve as a biocarrier for coral
in aquariums, provided coral acceptability, optimal feeding rate, and digestibility of the nauplii are well understood. Feeding
rate and digestibility of coral fed on A. salina nauplii at 100, 2,000, 4,000, 6,000, and 10,000 ind. l−1 under light and dark conditions was assessed in this study. The maximum feeding rates of Galaxea fascicularis under light and dark conditions was 113.6 ind. polyp−1 h−1 and 76.9 ind. polyp−1 h−1, respectively. The daily feeding rates of G. fascicularis varies and depends on nauplii density. Light plays an important role in coral feeding. Nevertheless, the quantity of A. salina nauplii consumed by the coral under light and dark conditions was not significantly different (P > 0.05). A. salina nauplii are well accepted by G. fascicularis. Complete nauplii digestion was observed after 180 min. Digestibility of A. salina nauplii by G. fascicularis was positively correlated with digestion time. 相似文献
18.
鳢流行性溃疡综合征病原分离鉴定与病理形态学观察 总被引：1，自引：0，他引：1
为寻找冬春季鳢(Channa maculata)烂身病的病原,应用组织压片、组织切片、扫描电镜技术、HE 常规染色法和 Grocott 六胺银染色法、霉菌分离纯化及 ITS 的序列分析等对冬春季患疑似溃疡综合征的烂身病鳢进行了病原学与病理学研究。病变组织压片观察到大量直径 10~20 μm,分枝较少、纤细菌丝。扫描电镜观察到肌肉组织中延伸出大量的纤细的丝状真菌。患病鳢皮肤和肌肉表现为变性、坏死与炎性细胞浸润,溃疡灶肌肉内可见大量的慢性肉芽肿结节和炎性细胞浸润。结节基本结构由类上皮细胞和多核巨细胞、炎性细胞、霉菌菌丝(横断面呈圆形,斜断或纵断呈丝状)组成,霉菌位于结节中央。Grocott 六胺银染色观察到结节中有大量棕色菌体。无菌分离培养可观察到典型丝状霉菌;霉菌在灭菌池塘水 20℃过夜, 12 h 后可观察原代孢子群形成,真菌 ITS 序列分析表明其与侵袭丝囊菌(Aphanomyces invadans)同源性为 100%。侵袭丝囊霉菌(A. invadans)是杂交鳢溃疡综合症病的主要病原,为鱼类流行病学调查和疾病防治的深入研究奠定基础。 相似文献
19.
Óscar Monroig Juan Carlos Navarro Isabel Amat Pedro González Francisco Amat Francisco Hontoria 《Aquaculture International》2003,11(1-2):151-161
Different liposome formulations, includingseveral combinations of membrane composition,type of vesicle (multilamellar and largeunilamellar vesicles), preparation method, andvehiculated nutrient, have been assayed asbioencapsulation products to enrich Artemia nauplii with nutrients for feeding fish larvae.The stability of the liposome preparationsunder conditions of use as enrichment producthas been tested using water soluble fluorescentmarkers as leakage indicators. The content ofthe fatty acids and lipid classesbioencapsulated in Artemia nauplii withliposomes has been analyzed by gas and thinlayer chromatography, respectively, andcompared with other enrichment products. Theeffect of the liposome enriched Artemianauplii used as food for fish larvae has beenevaluated in sea bass cultures. Liposomes withhigh content in polyunsaturated fatty acidsleak out more than 50% of their aqueous phasein less than 2 hours, unless they arestabilized with cholesterol and formed as largeunilamellar vesicles. Such vesicles hold70% of the encapsulated material for 8 hours.Liposome enriched nauplii in this study reflectthe influence of the enrichment products,however, they are far from the commercialemulsion (Super Selco) in terms ofdocosahexaenoic acid content, except for thenauplii enriched with liposomes made of purekrill phospholipid extract by the method ofdetergent solubilization. The liposome enrichednauplii show a higher amount of polar lipids incontrast to the feed enriched with emulsions.The larvae fed liposome enriched nauplii haveonly a slightly lower docosahexaenoic acidcontent than those fed emulsion enrichednauplii. The results obtained confirm thesuitable potential use of liposomes as foodsupplement in larviculture. Problems andadvantages are discussed. 相似文献
20.
Ming-Chao Yu Zhuo-Jia Li Hei-Zhao Lin Guo-Liang Wen Shen Ma 《Aquaculture International》2009,17(4):377-384
The basal diet (C), with 0.20% medicinal herbs (M) and 0.30% Bacillus (BM1), with 0.10% medicinal herbs and 0.15% Bacillus (BM2), and with 0.30% Bacillus (B), was used to feed white shrimps (Litopenaeus vannamei) (1.91 ± 0.03 g) in order to assess survival, growth, body composition, and digestive enzyme activity. At the end of the
feeding trial, survival ranged from 95.83 to 98.33% with no significant difference (P > 0.05) among all groups. Growth measured as weight gain was significantly (P < 0.05) higher in shrimp fed with BM2, BM1, and M compared to that of C. However, no significant differences were found among
B, BM1, and M. In the case of specific growth rate, the shrimp fed with BM1 and BM2 exhibited significantly (P < 0.05) higher values than that of C. The contents of body moisture, crude protein, and ash seemed to be unaffected by the
feed supplements, though lipid content was found to be significantly (P < 0.05) different among the treatments. The shrimp fed with BM1 and BM2 had the lowest and highest lipid contents, respectively.
The digestive enzyme activity assessed using shrimp hepatopancreas revealed that the activities of amylase and protease in
shrimp fed with BM2 were significantly (P < 0.05) higher than those of C at the end of the 2nd and 6th weeks. However, better performance of the specific amylase activity
was shown by the shrimp fed with B at the end of the 8th week. 相似文献