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1.
泥蚶生产性育苗的技术研究 总被引：1，自引：0，他引：1
本文论述了泥蚶生产性育苗的高产技术,亲贝在室内人工暂养保熟,自然排放,以金藻,扁藻,小球藻为饵料,以筛选的滩泥作附着基,每天放干池水,定期筛泥洗苗,经７８天在４８０ｍ＾２水池育出平均壳长１．０９ｍｍ的泥蚶苗种１６５３９万粒,单位面积出苗量达到３４．４５万粒。 相似文献
2.
本文总结了紫石房蛤人工育苗，放流增殖技术的研究结果，６月中旬（水温１４．６－１６．５℃）采捕的亲贝，经升温促熟６－７天（水温１８－２１℃），生殖腺发育成熟，采用浓度１００×１０＾－６的氨海水浸泡法采卵，以细沙，筛绢网箱和棕帘等三种附着基立体采苗，单位水体平均出苗量２５６万粒／ｍ＾３壳长２－６ｍｍ）。采用流水法在具沙质底的室内水池进行中间培育，育成率达到７８％，稚贝日平均增长７６μｍ。将壳长５ｍｍ左 相似文献
3.
５００ｍ＾３水体育出虾夷扇贝商品苗１．２亿粒。虾夷扇贝幼虫培育最适水温１４－１５℃；以金藻和扁藻投喂效果较好。 相似文献
4.
１９９８年进行了虾夷扇贝控温育苗高产试验。亲贝室内人工暂养促熟,１６℃产卵孵化,１６．５℃幼虫培育,投喂金藻３０１１,８７０１和扁藻,１４天投放附着基,稚贝培育１８天,中间保苗７０天,出苗量达到４２．１９万粒／ｍ＾３。 相似文献
5.
泥蚶工厂化育壮苗技术研究 总被引：1，自引：0，他引：1
利用普通扇贝育苗场进行泥工厂化育苗壮苗试验，亲蚶在室内丰饵能、成熟后自然排放，采卵孵化。幼虫、稚贝以叉鞭金藻、小球藻为饵料。以筛滤的滩泥作附着基，每天换水放干氏，定期换泥洗苗。１９９６年在底面积４８０ｍ＾２水泥池中，育出平均壳长１．０９ｍｍ的蚶苗１６５３９万粒。１９９６年１１月专家鉴定认为，技术水平居国内领先。 相似文献
6.
紫石房蛤增殖技术研究 总被引：3，自引：0，他引：3
本文总结了紫石房蛤人工育苗、放流增殖技术的研究结果。６月中旬（水温１４．６～１６．５℃）采捕的亲贝，经升温促熟６～７天（水温１８～２１℃），生殖腺发育成熟。采用浓度１００×１０－６的氨海水浸泡法采卵，以细沙、筛绢网箱和棕帘等三种附着基立体采苗，单位水体平均出苗量２５６万粒／ｍ３（壳长２～６ｍｍ）。采用流水法在具沙质底的室内水池进行中间培育，育成率达到７８％，稚贝日平均增长７６μｍ。将壳长５ｍｍ左右的苗种放流到适宜海区进行增殖，放苗密度１５０万粒／ｈａ。２周年后，紫石房给平均壳长达７３ｍｍ，鲜贝平均重１２３ｇ．平均每公顷产量２４．９７ｔ，回捕率１４％。 相似文献
7.
利用对虾育苗池进行泥蚶育苗试验报告 总被引：1，自引：0，他引：1
利用对虾育苗池进行泥蚶产卵孵化和幼虫培养试验,在４８０ｍ＾２育苗水体中,经过４５天,育出平均壳高３５３μｍ的泥蚶稚贝２．３亿粒,平均出苗密度４７．９２万粒／ｍ６２,最高达６３．５万粒／ｍ＾２,创产值６５万元、纯利３８万元。试验证明利用对虾育苗池进行泥蚶人工育苗是可行的。 相似文献
8.
泥蚶大面积工厂化育苗高产技术研究 总被引：1，自引：0，他引：1
选择浙江虾池促育熟肥的肥满足度７．０％左右的亲蚶,高密度采卵,低密度孵化,以海泥作附着基,整池移入变态附着池,分批附着。３－５天倒池洗苗一次,以扁藻,金藻,用角毛藻为主,辅投异胶藻,小球藻。１９９７年在１７４６ｍ＾２６能苗池中育出４５０－６３０μｍ＊３７６－５７０μｍ蚶苗１８．５７亿粒,平均单位面积出苗量１０６．３６万粒／ｍ＾２,效益显著,专家鉴定认为,本研究居国内领先水平。 相似文献
9.
研究了美国青蛙的形态,生态、人工催产,孵化,幼蛙培育,成蛙养殖,配饵驯食及腐皮病,红腿病,气泡病的防治技术,结果：孵化密度４０００－８０００粒／ｍ＾２,４８ｈ出苗。幼蛙培育适宜密度为８０－１００只／ｍ＾２,幼蛙体重１５ｇ左右时,以活饵带动法或直接驯食法分３个阶段进行驯食。成蛙养殖密度以２０－３０只／ｍ＾２为宜。５２ｍ＾２土池放养幼蛙１４０４只,经１２３天养殖,成蛙平均体重２５２ｇ,每平方米蛙６．３ 相似文献
10.
本次试验利用塑料大棚水泥地面积４５０ｍ＾２，将２０万尾异育银鲫水花培育成优质夏花。培育期为２７ｄ，共出夏花鱼种１８．２万尾，成活率达９１％，平均体长超过３．０ｃｍ，产值１８２００元，纯利润７０００元。 相似文献
11.
Liping Li Rui Wang Wanwen Liang Xi Gan Ting Huang Yan Huang Jian Li Yunliang Shi Honglin Luo Ming Chen 《广西水产科技》2014,(1)
Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China. 相似文献
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Growth hormone (GH) and reproduction: a review 总被引：5，自引：0，他引：5
Florence Le Gac Odile Blaise Alex Fostier Pierre-Yves Le Bail Maurice Loir Brigitte Mourot Claudine Weil 《Fish physiology and biochemistry》1993,11(1-6):219-232
Interaction between growth and reproduction occurs in many vertebrates and is particularly obvious at certain stages of the life cycle in fish. Endocrine interactions between the gonadotropic axis and the somatotropic axis are described, the potential role of GH being emphasised. A comparative analysis of these phenomena in mammals, amphibians and fish, suggests a specific role of GH in the physiology of puberty, gametogenesis and fertility. It also shows the original contribution made by studies on the fish model in this field of investigations. 相似文献
15.
Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL?1 (1.25 ng well?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation. 相似文献
16.
Controlled infection of Poecilia reticulata Peters (guppy) with Tetrahymena by immersion and intraperitoneal injection 
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下载免费PDF全文 G Sharon M Pimenta‐Leibowitz M C L Vilchis N Isakov D Zilberg 《Journal of fish diseases》2015,38(1):67-74
Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality). 相似文献
17.
Iciar Martinez Amaya Velasco Ricardo Pérez-Martín Eskil Forås Michiaki Yamashita Carmen G. Sotelo 《Journal Of Aquatic Food Product Technology》2013,22(4):289-297
The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus. 相似文献
18.
There has been growing concern about the overuse of antibiotics in the ornamental fish industry and its possible effect on the increasing drug resistance in both commensal and pathogenic organisms in these fish. The aim of this study was to carry out an assessment of the diversity of bacteria, including pathogens, in ornamental fish species imported into North America and to assess their antibiotic resistance. Kidney samples were collected from 32 freshwater ornamental fish of various species, which arrived to an importing facility in Portland, Oregon from Colombia, Singapore and Florida. Sixty‐four unique bacterial colonies were isolated and identified by PCR using bacterial 16S primers and DNA sequencing. Multiple isolates were identified as bacteria with potential to cause disease in both fish and humans. The antibiotic resistance profile of each isolate was performed for nine different antibiotics. Among them, cefotaxime (16% resistance among isolates) was the antibiotic associated with more activity, while the least active was tetracycline (77% resistant). Knowing information about the diversity of bacteria in imported ornamental fish, as well as the resistance profiles for the bacteria will be useful in more effectively treating clinical infected fish, and also potential zoonoses in the future. 相似文献
19.
Effect of iodophor disinfection of non‐hardened Salmo trutta eggs on their bacterial and fungus load 下载免费PDF全文
下载免费PDF全文 Franz Lahnsteiner 《Aquaculture Research》2017,48(7):3901-3909
This study investigated the efficiency of iodophor disinfection (135 ppm active iodine for 15–30 min) of non‐hardened Salmo trutta eggs against different groups of bacteria and against fungus. Egg samples were taken from non‐disinfected and from disinfected eggs, microorganisms were cultured on specific nutrient media and their mass was measured by turbidimetric methods. Bacteria and fungus mass of non‐hardened eggs could be reduced but not eliminated by iodophor disinfection with 135 ppm active iodine for 15 min. The extent of reduction was 47–65% (Experiment 1). The efficiency of disinfection increased with disinfection time as the reduction in bacteria and fungus mass was 40–55% after 15 min and 58–74% after 30 min (Experiment 2). Disinfection efficiency of iodophor solution diluted in water (reduction 49–57%) and of iodophor solution diluted in sodium chloride solution iso‐osmolar to the oocytes (reduction 52–61%) was similar (Experiment 3). The reduction in bacteria and fungus mass was persistent as it was 39–72% lower in embryos deriving from disinfected eggs than in embryos deriving from non‐disinfected ones (Experiment 4). In conclusion, the tested disinfection method is inadequate to eliminate pathogens completely but it could positively influence immune defence of eggs and embryos. 相似文献
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The endemic, anadromous cyprinidChalcalburnus tarichi is the only fish species known to occur in alkaline Lake Van (Eastern Anatolia, Turkey). EightC. tarichi were maintained individually in Lake Van water (17 – 19°C; pH 9.8; 153 mEq·I–1 total alkalinity; 22 total salinity) and tank water samples analyzed for 24 h in 2 to 4 h intervals. At zero time, < 1µM ammonia was present and urea was undetectable in the tank water; at 24 h, total ammonia and urea made up 114±32 and 35±25µM, respectively. Over the experimental period, ammonia-N and urea-N excretion averaged 1041±494 and 607±169moles·kg–1 fish·h–1, respectively. The extent of urea excretion was highly variable between specimens. Uric acid excretion was not detectable.Urea was present at high concentrations in all tissues and plasma (25 – 35moles·g–1·ml–1) of freshly caughtC. tarichi; total ammonia content of the tissues was by a factor of 1.9 (liver) to 3.0 (brain) lower. High arginase activity (2.4±0.2 U·min–1·g–1) was detected in the liver ofC. tarichi but ornithine carbamoylphosphate transferase, a key enzyme of the ornithine-urea-cycle, was absent. Ureagenesis is likely through degradation of arginine and/or uricolysis. High glutamine synthetase activity (11±0.6 U·min–1·g–1) and low ammonia content in brain suggest that, like other teleosts,C. tarichi has an efficient ammonia detoxification in the brain, but in no other tissue.Nitrogenous waste excretion at alkaline pH is discussed. The ability ofC. tarichi to excrete high levels of ammonia at extremely alkaline pH is unique among teleosts studied so far. The mechanism of ammonia excretion under Lake Van conditions remains to be elucidated. 相似文献