共查询到20条相似文献，搜索用时 515 毫秒
1.
Shengtao FAN Tiecheng WANG Xiaolong GAO Ying YING Xue LI Yongcheng LI Yuanguo LI Jinzhu MA Heting SUN Dong CHU Yu XU Songtao YANG Qihan LI Yuwei GAO Xianzhu XIA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1143-1149
Newcastle disease virus (NDV) causes a highly contagious viral disease in poultry and
wild birds, and it can cause significant economic loss worldwide. Eight viral strains were
isolated by inoculating embryonated chicken eggs from the Poyang Lake region of China with
swab samples. All eight of the NDV isolates were identified as class I genotype 3 strains,
but they diverged notablely from class II viruses. Further analysis revealed that all
eight NDV isolates were lentogenic strains containing the
112ERQER↓L117 motif at the F protein cleavage site. The strains
were highly identical and were more species specific (chicken and waterfowl) than site
specific (Nanchang and Duchang regions). The close phylogenetic proximity of these
isolates indicates that viral transmission may happen between poultry and wild birds. Our
study demonstrates that lentogenic class I NDVs exist in clinically healthy wild waterfowl
and poultry within the Poyang Lake region. Active surveillance of these viruses to
determine their evolution and origin is one of the most realistic strategies for
preventing and controlling NDV outbreaks. 相似文献
2.
During an epornitic of velogenic viscerotropic Newcastle disease (VVND) in southern California, free-flying wild birds, captive and free-ranging semidomestic birds, and exotic birds were collected from the quarantine area to determine their role in the epizootiology of the disease. The VVND virus was isolated from 0.04% of 9,446 free-flying wild birds, 0.76% of 4,367 semidomestic birds, and 1.01% of 3,780 exotic birds examined. Three house sparrows and 1 crow directly associated with infected poultry flocks were the only free-flying wild birds from which VVND virus was isolated. Among semidomestic species, ducks, quail, chukars, pheasants, peafowl, pigeons, and doves were found to be infected. Psttacines, pittas, and toucans accounted for 92% of the VVND virus isolations from exotic birds. In addition, domestic Newcastle disease virus (NDV) was isolated from 0.29% of the free-flying wild birds, from 1.65% of the semidomestic birds, and from 0.19% of the exotic birds collected. Hemagglutination-inhibition against domestic NDV was demonstrated in 0.24% of 3,796 wild bird serums, 8.28% of 2,004 semidomestic bird serums, and 3.90% of 231 exotic bird serums tested. Although few free-flying wild birds were infected with VVND virus in this epornitic, the isolation of domestic NDV strains from free-flying wild ducks and mourning doves suggests the potential for transportation of NDV over long distances by migratory birds. 相似文献
3.
To expand the epidemiological understanding of Newcastle disease virus (NDV) found in domestic ducks in Korea, 14 NDV isolates from apparently healthy domestic ducks were biologically and genetically characterized. Thirteen and 1 isolates of NDV were categorized into lentogenic and velogenic viruses, respectively, based on in vivo pathogenicity tests. Twelve lentogenic viruses showed HA activity to horse RBCs, while 1 lentogenic virus and the velogenic virus were negative. Lentogenic viruses (n=13) had sequence motifs of (112)ERQERL(117) (n=1) or (112)GRQGRL(117) (n=12) at the F0 cleavage site, while the velogenic virus (n=1) had a sequence motif of (112)RRQKRF(117) at the same site. Phylogenetic analysis revealed that at least three distinct genotypes may exist in domestic ducks in Korea; one class I genotype (genotype 2), and two class II (genotypes I and VII) genotypes. The class I virus was most closely related to strains of genotype 2 which were isolated in birds from the USA, Germany and Denmark. Twelve lentogenic class II viruses were grouped together in genotype I, and were then divided into at least three clusters, namely Aomori-like, Ulster2C-like, and V4-like. The velogenic class II virus was assigned to genotype VII which represents viruses responsible for recent epidemics in many Asian countries including Korea. The epidemiological importance of domestic duck isolates of NDV in Korea is discussed. 相似文献
4.
用RT-PCR方法扩增从野鸭体内分离到的3株新城疫病毒(newcastle disease virus,NDV)(JS-1/06/wd、JS-2/06/wd和JS-3/06/wd)F基因,并对其序列进行了测定和分析。结果表明该分离株的F基因长1 662 bp,编码553个氨基酸;F基因裂解位点的氨基酸序列为112R-R-Q-K/R-R-F117,符合NDV强毒株的特征。这些毒株间核苷酸同源性为99.8%~99.9%,与鹅源NDV QY971株和ZJ1株的核苷酸同源性也高达96.7%~97.5%;氨基酸同源性为96.8%~97.5%;而与我国标准强毒株F48E9及疫苗株LaSota的核苷酸同源性分别为86.9%和84.5%;氨基酸同源性分别为94.5%和87.0%。系统发育树分析结果表明,野鸭源NDV与鹅源NDV的遗传关系较近,同属于基因Ⅶ型。 相似文献
5.
Cai S Li J Wong MT Jiao P Fan H Liu D Liao M Jiang J Shi M Lam TT Ren T Leung FC 《Veterinary microbiology》2011,152(1-2):46-54
Complete genomes of four Newcastle disease virus (NDV) strains, isolated from ducks and wild birds in Guangdong province of China from 2003 to 2007, were sequenced and analyzed in this study. Pathogenicity tests in chicken embryos and chickens illustrate that D3 and R8 are lentogenic, and W4 and P4 are mesogenic strains. Phylogenetic analysis using all six genes provides a high resolution profile for genotype designation as genotype I for D3 and R8 strains and genotype VI for W4 and P4 strains. In addition, molecular dating based on different genes suggests that D3 and R8 diverged from their common ancestor at around 1998; W4 and P4 diverged from their common ancestor at around 1999. Subsequent selective pressure analysis displayed specific traits of genes evolution in all 4 strains since their divergence from the recent common ancestor. Furthermore, the geographic origins of 4 strains were deduced to be from Europe via two independent introduction events by phylogeographical analysis. This provides insights to the potential influence of waterfowl migration on NDV epidemiology. 相似文献
6.
近年来,新城疫逐渐成为了一种严重的家养水禽疾病。尽管如此,目前对于我国水禽新城疫的流行和分布情况依然知之甚少。本研究采集了2011-2012年中国华东6省1市的部分活禽市场和养殖场表观健康家鸭和家鹅泄殖腔棉拭样品,进行新城疫病毒的分离和毒力检测工作。通过测定分离株F基因高变区nt47-420,对其进行遗传进化分析。结果显示,在1200份样品中,检测到阳性样品23份,分离率为1．92％,表明眼观健康家鸭和家鹅中携带新城疫病毒。对比寒冷的春冬两季和炎热的夏季,新城疫病毒的分离率存在明显的差别。在中国家鸭和鹅中流行的新城疫毒株依然对温度敏感,高温天气不利于新城疫疫情蔓延。对分离株的鸡胚平均死亡时间和脑内接种指数进行了测定。参考76株GenBank中已发表并鉴定基因型毒株的F基因高变区nt47～420,对23株家养水禽分离株进行了遗传进化分析,结果发现分离株与流行的强毒株和疫苗株遗传距离较远。其中,13株与ClassII基因Ib亚型遗传距离最近;3株属于ClassI基因2型;7株与ClassI基因3b亚型遗传距离最近。 相似文献
7.
为跟踪调查广西活禽市场新城疫病毒(Newcastle disease virus,NDV)的流行分布和分子演化特征,2018年在广西不同城市10个活禽市场随机采集鸡、鸭、鹅和鸽子泄殖腔和咽喉拭子样品2820份,通过病毒分离与F基因扩增和测序,分析其遗传进化特征。结果显示:从4种宿主中共分离得到54份NDV,包含CLASSⅠ基因1b亚型、CLASSⅡ基因Ⅰ型和CLASSⅡ基因Ⅻ型三种基因型,且发现同一宿主存在不同基因型混合感染的情况。CLASSⅠ基因1b亚型NDV占比最大(50/54,92.5%)。遗传进化分析显示,广西分离株与同基因型参考株处于不同的小分支,存在地域差异。 相似文献
8.
Tsegaw Fentie Alireza Heidari Roberta Aiello Tesfu Kassa Ilaria Capua Giovanni Cattoli Mesfin Sahle 《Tropical animal health and production》2014,46(2):299-304
Newcastle disease (ND) is a highly contagious disease that affects many species of birds and causes significant economic losses to the poultry industry worldwide. Fifteen Newcastle disease virus (NDV) isolates obtained from rural chickens in northwest Ethiopia in 2011 and 2012 were characterized genotypically. The main functional region of the F gene was amplified and sequenced (260 nucleotides). Among the Ethiopian NDV isolates, 2 isolates had the virulent motif 112R-R-Q-K-R-F117 at the cleavage site of the fusion protein while 13 isolates contained the lentogenic motif 112G-G/R-Q-G-R-L117. Phylogenetic analysis based on the variable region of the F gene indicated that the two isolates exhibiting the virulent motif belonged to lineage 5 (genotype VII) subgenotype d and the remaining 13 isolates were grouped into lineage 2 (genotype II). The nucleotide sequences of lineage 5 isolates were genetically related to the Sudanese NDV isolates, suggesting potential epidemiological link of ND outbreaks between neighbouring countries. The lentogenic strains shared similarities with La Sota vaccine strain and probably originated from the vaccine strain either through direct exposure of birds to the live vaccine or to infectious La Sota-like strains circulating in rural poultry. This study provides genetic evidence on the existence of different NDV genotypes circulating in the rural poultry in Ethiopia. The virulent NDV continues to be a problem in poultry sector in Ethiopia, and their continuous circulation in rural and commercial poultry calls for improved surveillance and intensified vaccination and other control measures. 相似文献
9.
ClassⅠ新城疫病毒概述 总被引：1，自引：0，他引：1
新城疫病毒是影响养禽业健康发展的主要病原之一。新城疫病毒只有一个血清型,但根据亲源关系可以划分为ClassⅠ和ClassⅡ两大类。ClassⅠ新城疫病毒于2006年被证实存在,当前可被细分为9个基因型(1～9)。ClassⅠ所有自然分离株均为无毒株或弱毒株,但有经人工传代返强的报道,由于其分布广泛,数量庞大,对养禽业有巨大的潜在威胁。为了更好的了解ClassⅠ新城疫毒株的生物学特性,为研究ClassⅠ新城疫病毒提供参考,现将其基因组结构、分子流行病学及检测方法等综述如下。 相似文献
10.
During the latter stages of the lethal H5N2 influenza eradication program in domestic poultry in Pennsylvania in 1983-84, surveillance of waterfowl was done to determine if these birds harbored influenza viruses that might subsequently appear in poultry. From late June to November 1984, 182 hemagglutinating viruses were isolated from 2043 wild birds, primarily ducks, in the same geographical area as the earlier lethal H5N2 avian influenza outbreak. The virus isolates from waterfowl included paramyxoviruses (PMV-1, -4, and -6) and influenza viruses of 13 antigenic combinations. There was only one H5N2 isolate from a duck. Although this virus was antigenically related to the lethal H5N2 virus, genetic and antigenic analysis indicated that it could be discriminated from the virulent family of H5N2 viruses, and it did not originate from chickens. Many of the influenza viruses obtained from wild ducks were capable of replicating in chickens after experimental inoculation but did not cause disease. These studies show that many influenza A virus strains circulating in waterfowl in the vicinity of domestic poultry in Pennsylvania did not originate from domestic poultry. These influenza viruses from wild ducks were capable of infecting poultry; however, transmission of these viruses to poultry apparently was avoided by good husbandry and control measures. 相似文献
11.
半番鸭源禽1型副粘病毒FM01株的分离鉴定与F蛋白基因分析 总被引：5，自引：0，他引：5
从表现类似新城疫症状的病死半番鸭中分离到1株病毒FM01株，经血凝及血凝抑制试验证实为禽1型副粘病毒、以SPF鸡胚测定其平均致死时间为113，4h，对1d雏鸡脑内接种致病指数为0．23，表明为温和型毒株。利用RT-PCR技术一次性扩增其F蛋白全基因，克隆到pMD 18-T质粒载体，测序后获得F基因全序列，并推导出其相应的氨基酸序列。FM01株的F蛋白基因完整的编码区全长1662bp，编码553个氨基酸，其裂解位点的氨基酸序列为112G-R-Q-G-R-L117，具有温和型毒株特有的氨基酸序列结构．与常见新城疫毒株的核苷酸同源性在88．4％～99.6％之间，氨基酸同源性在89．2％～99.1％之间。 相似文献
12.
新城疫病毒通用型实时RT-PCR检测方法的建立与应用 总被引：3，自引：0，他引：3
采用TaqMan方法,经引物和探针的设计、筛选及反应条件优化,研究了检测活禽和禽产品中新城疫病毒的通用型实时RT-PCR(RRT-PCR)方法。结果显示,对12株分别为速发型、中发型、缓发型和疫苗株新城疫病毒的尿囊液倍比稀释液的检测极限在10-5~10-7之间;建立的方法与常见禽类病毒无交叉反应,特异性良好;在检测人工感染肉鸡的脏器组织、咽喉、泄殖腔拭子中病毒的灵敏度同鸡胚分离试验基本一致;弱毒疫苗免疫鸡群在免疫后14 d,应用本方法不能从咽喉、泄殖腔拭子中检测到病毒;临床样品检测表明,该方法不仅可以检出中强毒力新城疫毒株,也可检出缓发型野毒株和疫苗毒株。 相似文献
13.
14.
为了解新疆野鸟新城疫病毒（Newcastle disease virus,NDV）感染情况,分析其分子特征和基因变异特点,防止新城疫的暴发和蔓延,本试验用9日龄SPF鸡胚进行病毒的分离传代,HA、HI试验和RT-PCR方法对位于"东非-西亚迁徙线"福海县的野鸟进行了NDV检测,分离到1株野鸟源NDV,并命名为NDV/Pintail/CH（XJ）/01/2016。结果表明,该NDV分离株F基因ORF长1 662 bp,编码553个氨基酸,F基因碱性裂解位点序列为112G-R-Q-G-R-L117,符合弱毒株特征;遗传进化分析显示,NDV/Pintail/CH（XJ）/01/2016与乌克兰鸽源分离株Doneck/3/968、比利时鸭源分离株Simeonovgrad核苷酸同源性均为99.7%,属于NDV ClassⅡ基因Ⅱ型。而上述3株病毒均与疫苗毒La Sota具有较高同源性（99.5%~99.8%）。本研究结果为家禽NDV外流进入自然环境提供了论据。 相似文献
15.
ZHANG Hui-min Muhamaitijiang&# T SHI Jun WU Gui-ling Gulizhati&# Adili JIA Er-ken SU Zhan-qiang RAN Duo-liang Shayilan&# Kayizha LAN Ling YAO Gang LIU Jian-hua 《中国畜牧兽医》2017,44(9):2755-2760
In order to understand inflection of Newcastle disease virus (NDV) of Xinjiang wild birds, through analysis of molecular characteristics and genetic variation, to prevent the outbreak and spread of Newcastle disease, isolation of the virus was performed in 9 days old specific pathogen free (SPF) chicken embryos, detected the wild birds in Fuhai county which was located in the migration line of East Africa-West Asia by HA, HI test and RT-PCR method. And then 1 strain of NDV from wild birds was isolated, named as NDV/Pintail/CH(XJ)/01/2016. As the result, ORF of F gene from the NDV isolate was 1 662 bp, encoding 553 amino acids. The motif of the cleavage site was 112G-R-Q-G-R-L117, which was consistent with the characteristics of avirulent NDV strains. The phylogenetic analysis showed that NDV/Pintail/CH(XJ)/01/2016 nucleotide sequence homologies of F gene between reference strain Doneck/3/968 and mule Simeonovgrad strain homology were high and reached 99.7%, it belonged to genotype Ⅱ of Class Ⅱ NDV. All of the three viruses had high homology to the vaccine strain La Sota,which were 99.5% to 99.8%. According to this research conclusion we provided evidence that poultry NDV outflowed into the natural environment. 相似文献
16.
T Müller A Hlinak R U Mühle M Kramer H Liebherr K Ziedler D U Pfeiffer 《Avian diseases》1999,43(2):315-319
The sightings and migration patterns of 65 bean (Anser fabalis) and 65 white-fronted geese (Anser albifrons) are reported. In the past, these geese were serologically screened for the occurrence of Newcastle disease virus (NDV) and other avian viral diseases by Hlinak et al. (3). Of the 130 birds originally tagged and serologically screened in 1991, 53 birds were resighted between 1991 and 1996. Most of the sightings were reported from main wintering and resting sites in Germany and The Netherlands. It is noteworthy that 19 of the 53 birds sighted had serologic evidence that they had been exposed to NDV before the time of marking in 1991. Although the origin of these infections in bean geese and white-fronted geese is still unknown, the sightings reported in this study indicate that, once infected, wild geese may be involved in the dissemination and spread of avian viral diseases, specifically Newcastle disease. The migration patterns of the wild geese provided further evidence that the main resting and wintering areas of migratory waterfowl are likely to be important for the inter- and intraspecies transmission of avian diseases, thereby representing risk areas for the poultry industry. 相似文献
17.
Selection of thermostable Newcastle disease virus progeny from reference and vaccine strains. 总被引：2，自引：0，他引：2
D J King 《Avian diseases》2001,45(2):512-516
In a study of low-virulence Newcastle disease virus (NDV) isolates from poultry, 38% of the isolates had a more thermostable hemagglutinin than the lentogenic reference strains B1 and La Sota or live vaccines derived from those strains. Whether those strains with a more thermostable hemagglutinin are truly indigenous or whether they could have originated from vaccines used in the flocks was unknown. Seven monovalent NDV vaccines of B1 or La Sota type and reference B1 and La Sota strains were heat treated at 56 C to select variants more thermostable than the parent virus. Four thermal treatment cycles were completed, and virus propagated from the second and fourth heat treatments was assayed for changes in thermostability and antigenicity. The hemagglutinin thermostability of all vaccine and reference strain variants increased from the initial < or =10 min to > or =120 min after four treatments. Antigenic changes evaluated by hemagglutination inhibition against NDV monoclonal antibodies identified changes in only the heat-treated La Sota strains. The results demonstrate that the field isolates with a more thermostable hemagglutinin could have been derived by selection from the heterogenous NDV populations in vaccine strains and that minor antigenic changes may be a result of that selection. 相似文献
18.
Newcastle disease (ND) is a highly contagious viral disease particularly of domestic poultry. Switzerland is currently declared free from ND. A serosurvey using an ELISA was performed to investigate infections with ND-Virus (NDV) in 260 Swiss laying hen flocks, 169 backyard poultry flocks and 1576 wild birds. For laying hen flocks, a stochastic model was applied to analyse the results from serological testing. Four laying hen flocks were identified as NDV-seropositive, and the true NDV seroprevalence in this population was most likely between 1.3 and 1.5%. NDV antibodies were also detected in five of the 169 backyard poultry-flocks. ND-antibody positive birds were found in 10% of all wild birds examined, with the highest proportions among cormorants, grebes, birds of prey, owls, and swifts. The study indicated that positive flocks must have been in contact with NDV strains causing sub-clinical infection, since no clinical signs had been observed. Moreover, trade of poultry or poultry eggs was considered to be an important factor associated with seropositivity in backyard poultry flocks. Contact to wild birds did not seem to be of major importance. 相似文献
19.
Response of domestic geese to lentogenic and velogenic strains of Newcastle disease virus 总被引：4，自引：0，他引：4
A total of 54 domestic white meat-type geese were included in vaccination/challenge trials to evaluate susceptibility to disease and humoral immune responses using the haemagglutination inhibition (HI) and virus neutralization (VN) tests against Newcastle disease (ND). Two groups of twenty geese, five weeks of age, were conjunctivally vaccinated with either 100 x 10(6) or 2.5 x 10(6) EID50 (egg infectious dose 50 per cent) per bird of live La Sota virus, respectively, and 14 geese remained unvaccinated. At 15 weeks of age all vaccinated geese and seven unvaccinated geese were subcutaneously injected with 0.5 ml of inactivated oil emulsion ND vaccine, whereas seven geese remained as negative controls. At an age of 20 weeks, all 54 geese were challenged with 10(8.0) EID50 per bird of the viscerotropic velogenic NDV strain Herts 33/56. Live virus application as well as the oil emulsion vaccine did not induce discernible clinical signs and have no detrimental effect on body weight gains. At days 1, 3, 5, 8, 13, 16, 20, 23 and 27 after the application of lentogenic vaccine pharyngeal and cloacal swabs were taken, after challenge samples were taken at days 2, 5 and 8. Lentogenic as well as velogenic virus were never reisolated. Low and shortlived antibody responses post vaccination were equally well measured in HI and VN tests. Only two out of seven unvaccinated but challenged geese developed signs of ND whereas all vaccinated/challenged geese remained normal but developed high to moderate levels of HI and VN antibodies. Since domestic geese do not readily excrete NDV's in detectable amounts and since they do not contain detectable amounts of the challenge virus fourteen days post challenge in their tissues the assumption is promoted that geese do not play a major role in the epidemiology of Newcastle disease. 相似文献
20.