共查询到20条相似文献，搜索用时 46 毫秒
1.
为获得甜樱桃PaGAST 基因的cDNA 序列,并预测该基因编码蛋白的结构与功能,以草莓FaGAST1 基因序列为探针,通过基于NCBI数据库中表达序列标签的电子克隆技术对甜樱桃PaGAST 基
因进行克隆。利用生物信息学方法对其编码蛋白的理化性质、疏水性和亲水性、信号肽序列、跨膜结构域、亚细胞定位及功能等方面进行分析。结果表明：甜樱桃PaGAST 基因长度为750 bp,开放阅读框长度为324 bp,编码107 个氨基酸,N末端存在信号肽序列,C末端含有保守的GASA结构域。由于PaGAST蛋白中含有的疏水性氨基酸残基较多,该蛋白具有跨膜螺旋区,是一种跨膜蛋白,且有10 个预测的蛋白激酶磷酸化位点。亚细胞定位分析表明,PaGAST蛋白分布在细胞膜外的可能性很大。功能预测显示,PaGAST 基因可能具有响应胁迫应答、信号转导和免疫应答方面的功能。进化分析显示甜樱桃PaGAST蛋白与桃的亲缘关系最近。在一定程度上为甜樱桃PaGAST 基因的克隆及功能鉴定奠定理论基础。 相似文献
2.
为获得甜樱桃PaGAST基因的cDNA序列,并预测该基因编码蛋白的结构与功能,以草莓FaGAST1基因序列为探针,通过基于NCBI数据库中表达序列标签的电子克隆技术对甜樱桃PaGAST基因进行克隆。利用生物信息学方法对其编码蛋白的理化性质、疏水性和亲水性、信号肽序列、跨膜结构域、亚细胞定位及功能等方面进行分析。结果表明:甜樱桃PaGAST基因长度为750 bp,开放阅读框长度为324 bp,编码107个氨基酸,N末端存在信号肽序列,C末端含有保守的GASA结构域。由于PaGAST蛋白中含有的疏水性氨基酸残基较多,该蛋白具有跨膜螺旋区,是一种跨膜蛋白,且有10个预测的蛋白激酶磷酸化位点。亚细胞定位分析表明,PaGAST蛋白分布在细胞膜外的可能性很大。功能预测显示,PaGAST基因可能具有响应胁迫应答、信号转导和免疫应答方面的功能。进化分析显示甜樱桃PaGAST蛋白与桃的亲缘关系最近。在一定程度上为甜樱桃PaGAST基因的克隆及功能鉴定奠定理论基础。 相似文献
3.
4.
赤霉素3-氧化酶(gibberellin 3-oxidases, GA3ox)是赤霉素(gibberellic acid, GA)生物合成途径中的关键限速酶之一。芒果中GA3ox基因的功能及其表达模式未见报道。本研究利用RACE技术克隆了一个芒果GA3-氧化酶基因(GA3ox),该基因全长cDNA序列为1 680 bp,编码氨基酸381个,开放阅读框(open reading frame, ORF)为1 146 bp,蛋白分子量为42.6 kD,等电点为5.13,不含信号肽,不含跨膜结构域。系统发育树分析发现,该基因编码的蛋白主要与开心果亲缘关系较近,其次为克莱门柚、甜橙、麻疯树等植物。通过拟南芥原生质体亚细胞定位分析发现,该基因主要定位在细胞质中。通过对乔化、矮化芒果品种不同发育时期的叶片的实时荧光定量PCR分析发现,该基因主要在矮化品种中表达量较高,在乔化品种各个时期表达量较低,且差异不大。矮化品种中主要在开花期表达量最高,坐果期7～8周含量最低,但都高于乔化品种的任何取样时期。该基因的克隆和表达分析为下一步功能的研究及其在芒果株形调控分子机制提供理论依据。 相似文献
5.
以大豆叶片总RNA为模板,利用RT-PCR方法,克隆获得大豆KCS基因序列。采用生物信息学方法对其进行预测分析,结果表明:大豆KCS基因的CDS序列长度为1533bp,编码510个氨基酸;大豆KCS基因编码的蛋白质理论分子量为57.1kD,等电点为9.03;该基因编码的蛋白具有两个完全跨膜结构;没有信号肽;其蛋白质二级结构中α螺旋占47.65%,无规则卷曲占35.88%,β折叠占16.47%;在进化关系上,与油茶、菟葵、苜蓿的亲缘关系相对较近,该研究结果可为大豆KCS基因结构和功能的进一步研究提供理论参考。 相似文献
6.
人参属植物鲨烯合酶编码基因及其氨基酸序列的生物信息学分析 总被引：1，自引：1，他引：0
利用生物信息学方法对GenBank中人参属9种植物鲨烯合酶的cDNA序列以及其编码的氨基酸序列的结构、理化性质、信号肽、疏水性、亲水性、亚细胞定位、跨膜结构域功能域和进化关系进行了初步预测和分析。结果表明：总体上人参属鲨烯合酶核苷酸序列相似性平均为96.245%,氨基酸相似性平均为95.5%。其二级结构预测结果显示9个鲨烯合酶氨基酸序列以α螺旋和无规则卷曲为主要组成部分。对9种人参属植物进行进化树分析发现可以分为2个亚族。对包括人参属9种植物在内的其他物种进行进化树分析可知,植物、动物、酵母分属不同的类群。通过分析人参属植物鲨烯合酶及其编码基因的生物信息学特征,可以为鲨烯合酶基因的克隆和遗传操作提供理论参考。 相似文献
7.
为获得无核白葡萄中多酚氧化酶(PPO)的基因序列,以无核白葡萄果实为试材,进行基因同源克隆,得到多酚氧化酶基因CDS全长序列,其完整的编码框为1 824 bp,编码607个氨基酸(GenBank登陆号为KP271928);系统进化分析可知,其与葡萄科植物PPO基因同源性最高;生物信息学方法发现该蛋白分子量为67 392.44 Da,等电点为6.42,具典型的酪氨酸家族结构域,无信号肽结构,但有一个跨膜结构域,为亲水性蛋白;二级结构预测发现,PPO具有CuA与CuB两个结合区,分别嵌入蛋白活性腔中。该研究为进一步从分子水平探讨多酚氧化酶与褐变的关系奠定理论基础。 相似文献
8.
为了研究CDPK基因的结构和功能,利用RT-PCR方法从白羊草中克隆得到BiCDPK基因,并对其进行生物信息学分析。结果表明：该BiCDPK基因cDNA长1048 bp,包含387 bp的开放阅读框,编码128个氨基酸。蛋白表现为弱酸性,且稳定、亲水,二级结构中以无规则卷曲和α -螺旋为主。亚细胞定位于细胞质中,无跨膜结构域,无信号肽。蛋白序列中存在1个丝氨酸磷酸化位点和3个苏氨酸磷酸化位点。进化分析结果表明,克隆的BiCDPK基因与玉米、小麦和水稻具有较高同源性,相似度分别是97%、86%、86%。为进一步研究BiCDPK基因的功能奠定了基础。 相似文献
9.
《分子植物育种》2017,(4)
通过RT-PCR和RACE技术,从荔枝胚性愈伤组织中克隆得到了SUMO结合酶1基因的全长cDNA序列,命名为LcSCE1。LcSCE1 cDNA全长为840 bp,CDS全长483 bp,预测可编码160个氨基酸。生物信息学分析发现:LcSCE1为带负电的亲水不稳定蛋白,没有信号肽和跨膜结构域,进化上,LcSCE1与麻风树SCE1蛋白亲缘关系最近。利用Real-time PCR技术研究了LcSCE1在不同组织部位的表达情况,发现该基因在"元红"荔枝各组织器官中均有表达,在新叶与芽中表达量最高,膨大期果核中该基因的表达量也显著高于成熟果核(p0.01)。此外,本研究还比较了该基因在醮核和正常核中LcSCE1的表达情况,发现它在醮核中的表达量显著高于正常核(p0.01)。本研究结果表明LcSCE1可能在荔枝生长发育及醮核产生过程中发挥着重要作用。 相似文献
10.
红色叶绿素降解产物还原酶(red chlorophyll catabolite reductase,RCCR)是叶绿素降解过程的关键酶,能将叶绿素降解过程中产生的红色叶绿素代谢物(Red chl catabolit)分解代谢为原初荧光叶绿素降解产物(Primary fluorescent chl catabolite)。本研究根据转录组测序得到RCCR部分片段信息设计简并引物,采取3'和5'cDNA末端快速克隆(RACE)的技术方法,分别在三个不同着色芒果品种(红色‘贵妃’品种,黄色‘金煌’品种,绿色‘桂七’品种)中进行RCCR基因全长cDNA序列的扩增克隆得到了芒果果皮RCCR基因的全长cDNA序列。结果发现三个着色品种cDNA序列只有几个碱基的差异,以黄色金煌品种为例分析可得全长cDNA序列为1 134 bp,开放阅读框为975 bp,编码324个氨基酸,分子重量为36.21 k D,等电点为5.13。通过生物软件预测得到了三种三级蛋白结构图。对NCBI上刊载的30种不同植物构建系统发育树发现该基因编码的蛋白与橙子、克莱门柚等水果植物的亲缘关系比其他植物物种的亲缘关系近。近年来,病毒诱导的基因沉默(VIGS)是研究植物基因功能的有力工具,为深入探究RCCR基因在叶绿素降解中的作用,本研究成功构建出RCCR-pTRV2基因沉默载体,为后续研究RCCR基因在芒果果实成熟及果皮着色过程中的作用机理提供理论依据。 相似文献
11.
Jens Jensen 《Euphytica》1979,28(1):47-56
Summary The high-lysine gene in Risø mutant 1508 conditions an increased lysine content in the endosperm via a changed protein composition, a decreased seed size, and several other characters of the seed. The designation lys3a, lys3b, and lys3c, is proposed for the allelic high-lysine genes in three Risø mutants, nos 1508, 18, and 19. Linkage studies with translocations locate the lys3 locus in the centromere region of chromosome 7. A linkage study involving the loci lys3 and ddt (resistance to DDT) together with the marker loci fs (fragile stem), s (short rachilla hairs), and r (smooth awn) show that the order of the five loci on chromosome 7 from the long to the short chromosome arm is r, s, fs, lys3, ddt. The distance from locus r to locus ddt is about 100 centimorgans. 相似文献
12.
G. H. Kroon 《Euphytica》1994,76(1-2):125-125
Summary
K x vadensis is a hybrid of K. blossfeldiana and K. marmorata obtained after doubling the number of chromosomes. 相似文献
13.
Hongyan WEI Jian ZHOU Jinguang LU Jingzheng SONG Qiongxi YU Zhihong JIANG 《Medicinal Plant》2019,(6):27-29
[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenol... 相似文献
14.
Summary Avoidance of rust fungi that was based on poor appressorium induction was previously found in Hordeum chilense. In the present study 95 accessions of Triticeae were screened for avoidance of Puccinia hordei. The percentage of appressorium formation per germinated spore ranged from 6 to 90%. On none of the 41 accessions of Aegilops, Agropyron, Elymus, Secale, Thinopyrum or Triticum studied was the rate of appressorium formation lower than 25%. Lower rates of appressorium formation were, however, found on accessions of wild barley species Hordeum brachyantherum, H. marinum, H. parodii and H. secalinum. Its implications in cereal breeding are discussed. 相似文献
15.
D. A. Bond G. J. Jellis G. G. Rowland J. Le Guen L. D. Robertson S. A. Khalil L. Li-Juan 《Euphytica》1993,73(1-2):151-166
Progress is being made, mainly by ICARDA but also elsewhere, in breeding for resistance to Botrytis, AScochyta, Uromyces, and Orobanche; and some lines have resistance to more than one pathogen. The strategy is to extend multiple resistance but also to seek new and durable forms of resistance. Internationally coordinated programs are needed to maintain the momentum of this work.Tolerance of abiotic stresses leads to types suited to dry or cold environments rather than broad adaptability, but in this cross-pollinated species, the more hybrid vigor expressed by a cultivar, the more it is likely to tolerate various stresses. 相似文献
16.
Chunli TANG Fengxian ZHAO Hongxia CHEN Jiabao MA Jiangcun WEI Meiyan QIU Zhen XIE 《Medicinal Plant》2019,(6):60-65
[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination... 相似文献
17.
E. Keep 《Euphytica》1986,35(3):843-855
Summary Cytoplasmic male sterility (cms) is described in the F1 hybrids Ribes × carrierei (R. glutinosum albidum × R. nigrum) and R. sanguineum × R. nigrum. In backcrosses to R. nigrum, progenies with R. glutinosum cytoplasm were either all male sterile, or segregated for full male fertility (F) and complete (S) and partial (I) male sterility. Ratios of F:I+S suggested that two linked genes controlled cms, F plants being dominant for one (Rf
1) and recessive for the other (Rf
2).Segregation for cms in relation to three linded genes, Ce (resistance to the gall mite, Cecidophyopsis ribes), Sph
3(resistance to American gooseberry mildew, Sphaerotheca mors-uvae) and Lf
1(one of two dominant additive genes controlling early season leafing out) indicated that Rf
1and Rf
2were in this linkage group. The gene order and approximate crossover values appeared to be: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXafv3ySLgzGmvETj2BSbqef0uAJj3BZ9Mz0bYu% H52CGmvzYLMzaerbd9wDYLwzYbItLDharqqr1ngBPrgifHhDYfgasa% acOqpw0xe9v8qqaqFD0xXdHaVhbbf9v8qqaqFr0xc9pk0xbba9q8Wq% Ffea0-yr0RYxir-Jbba9q8aq0-yq-He9q8qqQ8frFve9Fve9Ff0dme% aabaqaciGacaGaamqadaabaeaafaaakeaacaWGdbGaamyzamaamaaa% baGaaiiiaiaacccacaGGWaGaaiOlaiaacgdacaGG0aGaaiiiaiaacc% caaaGaaiiiaiaacccacaGGGaGaamOuaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaaccdacaGGUaGaaiOmaiaacs% dacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaaaacaWGsbGaamOzaSGa% aGOmaOWaaWaaaeaacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccaaaGaamitaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccacaGGGaaaaiaadofacaWGWbGaamiAaSGa% aG4maaaa!6E4D!\[Ce\underline { 0.14 } Rf1\underline { 0.24 } Rf2\underline { } Lf1\underline { } Sph3\]. Crossover values of 0.36 for Ce-Lf
1, and 0.15 for Lf
1-Sph
3were estimated from the relative mean differences in season of leafing out between seedlings dominant and recessive for Ce and Sph
3.It is suggested that competitive disadvantage of lf
1-carrying gametes and/or zygotes at low temperatures may be implicated in the almost invariable deficit of plants dominant for the closely linked mildew resistance allele Sph
3. Poor performance of lf
1- (and possibly lf
2-) carrying gametes and young zygotes during periods of low temperature at flowering might also account for the liability of some late season cultivars and selections to premature fruit drop (running off). 相似文献
18.
[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and ortho... 相似文献
19.
Summary Apple and pear pollen was irradiated with doses of 0, 50, 100, 250 and 500 krad (gamma rays) and stored at 4°C and 0–10% r.h. From the in-vitro germination percentages an average LD 50 dose of about 220 krad was estimated. For both irradiated and untreated pollen a close and corresponding lineair relationship existed between germination percentage and pollen tube growth.Irradiated pollen was much more sensitive to dry storage conditions than untreated pollen, resulting in less germination and more bursting. Apparently, irradiation caused the pollen cell membrane to lose its flexibility faster than normal. Rehydration of dry-stored, irradiated pollen in water-saturated air restored germination percentages up to their initial levels. The importance of this procedure in germination trials is stressed. 相似文献
20.
Parasitic angiosperms cause great losses in many important crops under different climatic conditions and soil types. The most widespread and important parasitic angiosperms belong to the genera Orobanche, Striga, and Cuscuta. The most important economical hosts belong to the Poaceae, Asteraceae, Solanaceae, Cucurbitaceae, and Fabaceae. Although some resistant cultivars have been identified in several crops, great gaps exist in our knowledge of the parasites and the genetic basis of the resistance, as well as the availability of in vitro screening techniques. Screening techniques are based on reactions of the host root or foliage. In vitro or greenhouse screening methods based on the reaction of root and/or foliar tissues are usually superior to field screenings and can be used with many species. To utilize them in plant breeding, it is necessary to demonstrate a strong correlation between in vitro and field data. The correlation should be calculated for every environment in which selection is practiced. Using biochemical analysis as a screening technique has had limited success. The reason seems to be the complex host-parasite interactions which lead to germination, rhizotropism, infection, and growth of the parasite. Germination results from chemicals produced by the host. Resistance is only available in a small group of crops. Resistance has been found in cultivated, primitive and wild forms, depending on the specific host-parasite system. An additional problem is the existence of pathotypes in the parasites. Inheritance of host resistance is usually polygenic and its transfer is slow and tedious. Molecular techniques have yet to be used to locate resistance to parasitic angiosperms. While intensifying the search for genes that control resistance to specific parasitic angiosperms, the best strategy to screen for resistance is to improve the already existing in vitro or greenhouse screening techniques. 相似文献