共查询到20条相似文献，搜索用时 31 毫秒
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Meenakshi M. Bakshi C.S. Butchaiah G. Bansal M.P. Siddiqui M.Z. Singh V.P. 《Veterinary research communications》1999,23(2):81-90
The immunogenicity of a sonicated extract (SE) and of outer membrane proteins (OMP) of Salmonella enteritidis was tested in birds of about 8 weeks of age. The dose, route of vaccination and the adjuvant used varied in different groups of birds. Two vaccine doses with or without adjuvant were given parenterally or orally 3 weeks apart. OMP vaccines gave significantly higher antibody titres than SE vaccines, as indicated by ELISA. The vaccines adjuvanted with oil produced higher antibody titres than those without any adjuvant. A dose of 1 mg of vaccine produced higher antibody titres than 0.5 mg of vaccine. Adjuvanted vaccine given subcutaneously elicited higher antibody responses than oral vaccines given without adjuvant. The birds were challenged with virulent S. enteritidis organisms at the end of the second week after a booster dose. None of the birds given 1 mg of OMP vaccine subcutaneously shed the organisms when tested by culturing cloacal swabs, although a few birds vaccinated with 0.5 mg of OMP vaccine did so. In general, adjuvanted OMP vaccines gave better protection than SE vaccines. 相似文献
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为筛选出理想的鸭TMUV-AIV(H9亚型)二联灭活疫苗免疫佐剂,将灭活的坦布苏病毒液和禽流感病毒(H9亚型)尿囊液分别与白油佐剂、卡波姆佐剂、铝胶佐剂、蜂胶佐剂制成抗原含量相同的TMUV-AIV二联灭活疫苗。应用阻断ELISA方法和血凝抑制方法测定免疫鸭血清中坦布苏病毒和禽流感病毒抗体,比较不同佐剂疫苗对试验鸭的免疫效力。结果表明,以白油作为佐剂的灭活疫苗组免疫效果最好,抗体产生快、抗体水平优于其他各组,免疫2周后60%(6/10)血清样品HI抗体可达1∶8以上,80%血清样品呈TMUV抗体阳性,是制备鸭TMUV-AIV(H9亚型)二联灭活疫苗的理想佐剂。 相似文献
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作者制备了3批牦牛大肠埃希氏菌病蜂胶疫苗。制备了蜂胶佐剂和培养基,进行了牦牛大肠埃希氏菌的菌液培养、纯粹检验、活菌计数、灭活及无菌检验,配苗、分装及成品检验。3批牦牛大肠埃希氏菌病蜂胶疫苗无菌检验为阴性;物理性状检验为：静置后上层是乳黄色液体,下层为土黄色沉淀,振荡后呈均匀混浊液;经家兔安全检验结果为安全;经家兔效力试验,免疫后家兔抗体效价≥12．709lg2,对家兔具有免疫保护作用;蜂胶疫苗经防冻试验证明,随着蜂胶含量的增高,防冻效果越好。本试验为西藏牦牛大肠埃希氏菌病的预防提供依据。 相似文献
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为研究比较5种塞内卡病毒疫苗佐剂,分别用4种自主研发佐剂和进口ISA206佐剂配制塞内卡病毒灭活疫苗,检测疫苗的理化性质、安全性及免疫效力,并对检测结果进行对比分析。结果显示,进口ISA206佐剂疫苗黏度为43.02 cP,自主研发佐剂疫苗黏度均在27.88 cP以下,其他理化性质无差异;5批疫苗安全性试验均未见明显异常;5批疫苗免疫动物后均可诱导机体产生中和抗体,二免后14 d抗体滴度在211以上,4批自主研发佐剂疫苗免疫组抗体滴度水平高于进口ISA206佐剂疫苗约1个滴度,攻毒保护结果均在4/5以上,2批自主研发佐剂疫苗免疫组保护率可达5/5。结果表明,用4种自主研发佐剂制备的塞内卡病毒灭活疫苗质量不低于用进口ISA206佐剂制备的塞内卡病毒灭活疫苗。 相似文献
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为了探讨不同佐剂的奶牛乳房炎多联苗对小鼠免疫机能的影响,笔者将小白鼠分为3个组,每组30只,分别为蜂胶苗组、铝胶苗组和空白对照组。用制备的氢氧化铝胶和蜂胶2种乳房炎佐剂多联苗分别免疫小鼠,分别于免疫后的7、14、21、30和45 d采血,进行2种多联苗免疫后E-玫瑰花环率和淋巴细胞转化率测定。结果表明,蜂胶和铝胶2种佐剂苗与空白对照组相比均能显著提高小鼠的细胞免疫水平,2种苗的细胞免疫水平均在免疫后30 d左右达到最高峰(P<0.01),但在免疫后第30和45天时E-玫瑰花环率和淋巴细胞转化率,蜂胶苗组均极显著高于铝胶苗组(P<0.01),说明蜂胶佐剂比铝胶佐剂能更强地增强多联苗抗原对小鼠T细胞的致敏程度。该研究为进一步明确乳房炎多联苗免疫机制及筛选更好的免疫佐剂提供了科学数据。 相似文献
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Antibody responses of sheep vaccinated with foot rot vaccines 总被引：1，自引：0，他引：1
Enzyme-linked immunosorbent assay (ELISA) and crossed immunoelectrophoresis (IEP) were used to investigate antibody responses of sheep vaccinated with a double adjuvanted or single adjuvanted commercial foot rot vaccine. ELISA detected an antibody response of greater magnitude to the double adjuvant vaccine compared with the single adjuvant vaccine. Sera from sheep vaccinated with double adjuvant vaccine recognised at least six antigens of Bacteroides nodosus in crossed IEP while sera from the single adjuvant vaccinated sheep recognised one antigen. The use of non-denatured antigens of B nodosus in ELISA and crossed IEP enabled quantitative comparisons of antibody responses to the different foot rot vaccines to be made. 相似文献
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疫苗免疫在禽病控制上的进展 总被引：2，自引：0，他引：2
疫苗免疫已有１０００多年的历史,但只有进入二十世纪以后,疫苗科学才得到了迅速的发展。目前商品性疫苗主要是弱毒活疫苗,灭活疫苗和亚单位疫苗三大类,它们有各自的优点和不足。新一代的疫苗,如基因缺失疫苗,载体疫苗,核酸疫苗和病毒－抗体复合物疫苗等,将从质和量两方面改善其免疫应答和免疫保护,并导致新的疾病控制策略的出现。 相似文献
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M M Rweyemamu O Umehara D de Lucca-Neto M C Baltazar F E Vicente R da R Medeiros-Neto 《American journal of veterinary research》1986,47(6):1243-1248
Avridine, a lipoidal amine with interferon-inducing and adjuvant properties, was an effective adjuvant for Newcastle disease antigen (NDA) in chickens. Eleven vaccine lots were evaluated: 2 commercial water-in-oil vaccines, 4 experimental oil emulsion vaccines, 4 avridine-containing vaccines, and a control lot of nonadjuvanted antigen. Avridine significantly enhanced the immunologic responses of chickens against NDA. Chickens vaccinated with the avridine-containing vaccines had significantly higher antibody titers (hemagglutination inhibition) than did chickens vaccinated with the commercial vaccines. Experimental oil emulsion vaccines prepared from the same antigens as avridine-adjuvanted vaccines induced higher hemagglutination inhibition antibody titers after primary but not after booster vaccination. Use of avridine as an adjuvant for NDA in vaccines for chickens induced immunologic protection rates similar to those induced by oil emulsion vaccines, without causing the reactogenic and tissue residue problems associated with the use of oil vaccines in chickens. 相似文献
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Danyang Wang Yu Yang Jinyu Li Bin Wang Ailian Zhang 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(3)
BackgroundNew-generation adjuvants for foot-and-mouth disease virus (FMDV) vaccines can improve the efficacy of existing vaccines. Chinese medicinal herb polysaccharide possesses better promoting effects.ObjectivesIn this study, the aqueous extract from Artemisia rupestris L. (AEAR), an immunoregulatory crude polysaccharide, was utilized as the adjuvant of inactivated FMDV vaccine to explore their immune regulation roles.MethodsThe mice in each group were subcutaneously injected with different vaccine formulations containing inactivated FMDV antigen adjuvanted with three doses (low, medium, and high) of AEAR or AEAR with ISA-206 adjuvant for 2 times respectively in 1 and 14 days. The variations of antibody level, lymphocyte count, and cytokine secretion in 14 to 42 days after first vaccination were monitored. Then cytotoxic T lymphocyte (CTL) response and antibody duration were measured after the second vaccination.ResultsAEAR significantly induced FMDV-specific antibody titers and lymphocyte activation. AEAR at a medium dose stimulated Th1/Th2-type response through interleukin-4 and interferon-γ secreted by CD4+ T cells. Effective T lymphocyte counts were significantly elevated by AEAR. Importantly, the efficient CTL response was remarkably provoked by AEAR. Furthermore, AEAR at a low dose and ISA-206 adjuvant also synergistically promoted immune responses more significantly in immunized mice than those injected with only ISA-206 adjuvant and the stable antibody duration without body weight loss was 6 months.ConclusionsThese findings suggested that AEAR had potential utility as a polysaccharide adjuvant for FMDV vaccines. 相似文献
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采用人工感染兔瘟(兔病毒性出血症)病毒致死的兔的脏器(主要是肝)，制成10％的兔瘟组织灭活苗和10％的兔瘟蜂胶组织灭活苗。采用常规血凝抑制实验，初步分析比较了注射兔瘟组织灭活苗和兔瘟蜂胶组织灭活苗后兔体内抗体水平的消长情况。结果表明：兔瘟蜂胶组织灭活苗能明显提高抗体效价，增强免疫效果。 相似文献
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IgG antibody response in chickens immunized with F4 fimbriae extracted from local enterotoxigenic Escherichia coli (ETEC) strain was studied during a 98-day immunization period for comparing the efficacy of four adjuvants: Freund' adjuvant, Quil A (QA), propolis and extract from Cochinchina momordica seed (ECMS). For this purpose, chickens were immunized with F4 fimbriae alone or combined with one of the above adjuvants on days 1 and 21. IgG antibody levels in serum and egg yolk (by ELISA) were measured on days 0, 7, 14, 21, 28, 35, 42, 49, 56, 70, 84 and 98. The egg production of each group was also determined during days 1-7 and the following four weeks. The results showed that QA could enhance antibody titre, as good or almost as good as Freund's adjuvant, whereas the titres of ECMS and propolis groups were relatively lower, with the overall order: Freund's adjuvant>QA>ECMS>propolis both in serum and egg yolk. However, the significant decrease of egg production was merely observed in the Freund's adjuvant group. It is concluded that the four adjuvants tested can stimulate immune response to F4 fimbriae in chickens, with Freund's adjuvant giving the best results, followed by QA. 相似文献
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肠毒素性大肠杆菌菌毛对产蛋鸡免疫性的研究 总被引：4，自引：1，他引：3
采用热抽提法提取4种肠毒素性大肠杆菌菌毛蛋白。K88、K99、F41和987p菌毛蛋白分别制成弗氏佐剂苗;K88还制成白油佐剂苗,氢氧化铝胶苗和蜂胶佐剂苗;另将4种菌毛等比例混合制成弗氏佐剂苗。分别对产蛋鸡进行免疫,用微量凝集反应和血凝抑制试验检测卵黄抗体效价。结果表明,K88菌毛较其他3种菌毛免疫性好,诱导抗体效价最高而且能长时间维持;987p菌毛能快速诱导抗体的产生,但整体效价低。K88不同佐剂苗中,铝胶佐剂能较快地诱导抗体的产生,蜂胶佐剂苗抗体持续时间短,弗氏佐剂能诱导高效价的抗体产生而且能长时间持续。 相似文献
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复方中药成分对新城疫疫苗免疫雏鸡外周血T淋巴细胞转化和血清抗体效价的影响 总被引：13，自引：0，他引：13
选择4种中药成分（黄芪多糖、淫羊藿多糖、峰胶黄酮、人参皂甙）组成2个复方，各按3个剂量水平与新城疫疫苗混合后免疫雏鸡，以无佐剂苗和油乳苗为对照，分别于免疫后7、14、21、28、35、42d采血，用MTT法和微量血凝法测定T淋巴细胞转化和血清抗体效价的动态变化。结果表明。中药佐剂能显著促进淋巴细胞转化。提高血清抗体效价，多数时间点与油佐剂的效果相当，部分时间点显著强于油佐剂。综合判定方1高剂量和方2中剂量的效果最好。 相似文献
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旨在筛选适宜于猪丹毒丝菌灭活疫苗的佐剂。以分离鉴定出的猪丹毒丝菌1a型HG-1株灭活菌体为抗原分别配制矿物油佐剂疫苗（简称矿物油疫苗）、氢氧化铝胶佐剂疫苗（简称铝胶疫苗）、ISA201双相油乳佐剂疫苗（简称ISA201疫苗）、GEL02水溶性聚合物佐剂疫苗（简称GEL疫苗）、IMS1313水溶性纳米佐剂疫苗（简称IMS1313疫苗）共5种佐剂的灭活疫苗。小鼠免疫保护试验结果表明,二免14 d后使用约为4 LD50的HG-1株对小鼠进行腹腔攻毒,矿物油疫苗和GEL疫苗的保护率分别为100%（7/7）和71%（5/7）,其他三种佐剂疫苗的保护率均为14%（1/7）。本研究进一步选择铝胶疫苗和GEL疫苗进行猪体对比试验;仔猪安全性试验结果表明,两种佐剂疫苗的副反应均较小;免疫保护试验结果表明,两次免疫后使用约为16 LD100的HG-1株对免疫仔猪进行耳缘静脉攻毒,两种佐剂疫苗的保护率分别为60%（3/5）和100%（5/5）。本研究最终选择GEL佐剂作为开发猪丹毒灭活疫苗的最适佐剂。 相似文献
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Chaung HC Cheng LT Hung LH Tsai PC Skountzou I Wang B Compans RW Lien YY 《Veterinary microbiology》2012,157(1-2):69-77
Flagellin, a bioactive Toll-like receptor (TLR) 5 ligand, may trigger the innate immunity that in turn is important for subsequent adaptive immune responses. In the present study, the adjuvant effects of the monomeric and polymeric forms of Salmonella flagellin (mFliC and pFliC, respectively) were examined in specific-pathogen free (SPF) chickens immunized intramuscularly (i.m.) or intranasally (i.n.) with formalin-inactivated avian influenza virus (AIV) H5N2 vaccines. Results showed that mFliC cooperating with the 64CpG adjuvant significantly induced influenza-specific antibody titers of plasma IgA in the i.m.-vaccinated animals. The nasal IgA levels in the i.n.-mFliC-coadministrated AIV vaccinated chickens were significantly elevated compared to levels observed in the control group (H5N2 vaccine alone). The pFliC cooperating with the 64CpG adjuvant significantly enhanced cell proliferation of splenocytes in the i.m.-vaccinated animals. TLR3 and TLR5 expressions were activated by flagellin stimulation in vitro and in vivo. These results suggest that flagellin can be used as an adjuvant in an AIV H5N2 vaccine, especially for mucosal immunity. 相似文献