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将副猪嗜血杆菌(Hps)4、5、13型分离菌株超声产物致敏经戊二醛和鞣酸处理的绵羊红细胞,建立了检测Hps抗体的间接血凝试验方法。对15种血清型Hps阳性血清进行检测,结果均为阳性,抗体效价达1∶25~1∶210,敏感性明显高于琼脂扩散试验。对其它16种病毒和细菌的阳性血清进行检测,结果除胸膜肺炎放线杆菌有凝集外,其余均为阴性。对620份临床血清进行检测,阳性率为67.58%。结果表明,该方法敏感性较高、特异性强,可用于Hps抗体水平的检测和流行病学调查。 相似文献
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用间接血凝试验检测猪传染性胸膜肺炎 总被引：20，自引：0，他引：20
经戊二醛化－鞣酸化处理的绵羊红细胞用胸膜肺炎嗜血杆菌（ＨＰ）抗原致敏,以间接血凝试验（ＩＨＡ）检测猪传染性胸膜肺炎病血清抗体。致敏红细胞抗原的最佳浓度为１００－１５０μｇ／ｍＬ,超免疫血清的抗体效价达１：５１２－１：１０２４,对人工感染１４ｄ的猪血清阳性检出率达８０％,与猪瘟、猪肺疫、喘气病、猪萎缩性鼻炎阳性血清无效叉反应。 相似文献
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《黑龙江畜牧兽医》2017,(13)
为了建立副猪嗜血杆菌(Haemophilus parasuis,HPS)平板凝集抗体检测方法,试验采用方阵试验方法对15种HPS菌体抗原和阳性血清的交叉凝集反应性进行了研究,从中筛选出能与所有血清型HPS血清发生凝集反应的多价抗原,并测定其特异性。结果表明:各血清型HPS抗原抗体之间均存在不同程度的交叉凝集反应,但没有任何一种血清型的HPS能与全部15种血清型抗体发生凝集反应,由各血清型的交叉凝集谱可见,血清1型、6型、7型等三个血清型抗原能覆盖全部15个血清型抗体,据此选择这三个菌株作为多价抗原生产菌株。将其接种于含NAD的TSA平板上,于37℃培养18 h,用pH值为7.2的PBS洗下菌苔并将菌液浓度分别调整至8×10~9cfu/m L,三种菌液用甲醛灭活后等体积混合即为HPS平板凝集多价凝集原,其能与15个血清型HPS抗体发生75%及以上的凝集反应,能凝集HPS的感染抗体和免疫抗体,不与猪传染性胸膜肺炎等被检的无关疫病的阳性血清发生反应。说明试验制备的多价平板凝集抗原能用于HPS抗体的检测和血清流行病学调查。 相似文献
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副猪嗜血杆菌的分离鉴定 总被引：10，自引：0，他引：10
副猪嗜血杆菌病是由副猪嗜血杆菌（HPS）引起猪的以纤维素性浆膜炎、多发性关节炎和脑膜炎为特征的传染病。随着养殖规模的不断扩大和引进国外种猪，副猪嗜血杆菌病在我国猪场的病发率逐年上升。HPS血清型复杂，目前已知15个血清型，但各国各地流行的血清型不尽相同，且各型之问缺乏交叉保护，因此分离HPS地方菌株，了解其流行血清型，对地区预防和控制HPS有着重大的临床意义。由于HPS对培养条件较为苛刻，临床病料分离培养HPS有一定的难度，目前国内分离HPS报道不多，本文对广东地区疑似副猪嗜血杆菌病病料分离并鉴定了3株副猪嗜血杆菌，报道如下。 相似文献
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为了解副猪嗜血杆菌(HPS)在石河子规模猪场的流行情况,试验采用ELISA方法进行了副猪嗜血杆菌抗体的检测,并对抗体阳性血清进行了其他呼吸道病混合感染血清学调查。结果表明:330份血清样品HPS抗体阳性率为60.30%(199/330),12个猪场感染阳性率为91.67%(11/12);199份阳性血清中,24.62%(49/199)感染了H1N1亚型猪流感病毒(SIV),49.25%(98/199)感染了猪肺炎支原体(Mhp),84.42%(168/199)感染了猪圆环病毒2型(PCV-2),35.18%(70/199)感染了猪胸膜肺炎放线杆菌(APP),49.75%(99/199)感染了猪链球菌2型(SS2)。说明垦区大部分规模猪场存在HPS感染情况,且混合感染情况比较严重。 相似文献
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《养猪》2018,(6)
为了解平顶山市养猪场户副猪嗜血杆菌病流行现状及其混合感染情况。采用ELISA方法首先对8个乡镇22个养殖场户562份血清进行副猪嗜血杆菌病检测,其中384份为副猪嗜血杆菌病阳性,然后对此384份副猪嗜血杆菌病阳性血清进行H1N1亚型猪流感病毒病、猪支原体肺炎、猪圆环病毒病2型、猪链球菌病2型、猪传染性胸膜肺炎抗体检测。结果表明,猪场副猪嗜血杆菌病群体流行率为72.73%(16/22),副猪嗜血杆菌病个体流行率为68.32%(384/562),384份副猪嗜血杆菌阳性血清中,H1N1亚型猪流感、猪支原体肺炎、猪圆环病毒病2型、猪链球菌病2型和猪传染性胸膜肺炎阳性率分别为35.42%(136/384)、47.39%(182/384)、54.17%(208/384)、38.54%(148/384)和48.96%(188/384)。平顶山市养猪场户副猪嗜血杆菌病及其混合感染情况比较严重,应引起当地兽医部门的高度重视。 相似文献
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采用Ni-NTA His.Bind方法纯化pET-28a-ApxⅣ2表达蛋白,以羧化乳胶为载体,经化学交联法致敏乳胶,对致敏条件优化后进行质量检测。结果显示,在pH 4.7的PBS缓冲液中,乳胶浓度为2%、蛋白质量浓度为1.5g/L,偶联8h后制备的致敏乳胶凝集反应最好,致敏乳胶抗原无自凝现象;与副猪嗜血杆菌、猪瘟病毒、猪伪狂犬病病毒、猪繁殖与呼吸综合征病毒、大肠杆菌和多杀性巴氏杆菌阳性血清不发生凝集;批内重复、批间重复试验很稳定;阳性血清敏感性可达1∶32。对比试验中与ELISA方法的符合率为80%,2种方法检测的结果基本相符。结果表明,试验初步建立了检测猪胸膜肺炎放线杆菌ApxⅣ毒素抗体的乳胶凝集方法,为猪传染性胸膜肺炎的快速诊断提供了技术支撑。 相似文献
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用猪链球菌血清2型(Streptococcus suis serotype 2,SS2)的荚膜多糖(Capsular polysaccharide,CPS)致敏经戊二醛处理过的鸡红细胞,优化致敏醛化红细胞的抗原量和时间.结果显示,在37℃条件下CPS(2.2 5 mg/L)致敏鸡红细胞90 min,IHA试验在25℃条件下操作,被检血清IHA效价在1∶8及其以上时判为SS2抗体阳性.应用此方法对猪大肠杆茵、副嗜血杆菌、猪肠球菌、SS1、SS7、SS9、金黄色葡萄球菌、沙门菌、巴氏杆茵阳性血清进行检测,结果表明,SS2抗体均为阴性.对1044份无临床症状的猪血清进行检测,SS2抗体阳性率为61.69％.该方法敏感性高,特异性较强,可用于大规模SS2抗体水平的检测和SS2的血清流行病学调查. 相似文献
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为了解江西省猪群猪传染性胸膜肺炎放线杆菌（APP）感染现状,本试验利用APP江西分离株研制了抗体间接血凝检测法（IHA）。试验将纯化的APP江西分离株培养菌液反复冻融,经超声波裂解处理后提取菌体抗原,致敏经戊二醛固定鞣酸化的兔红细胞,成功建立了APP抗体的IHA法。应用该法对猪瘟病毒、猪繁殖与呼吸综合征病毒、猪伪狂犬病病毒等病毒的阳性血清进行检测试验,结果均为阴性;对大肠杆菌、沙门氏菌、副猪嗜血杆菌阳性血清检测均为阴性。不同批次APP抗原经IHA检测,结果显示重复性好。本试验结果表明所建立的APP抗体IHA检测法有较好的特异性和重复性,为下一步血清学调查奠定基础。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献
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乳酸杆菌作为益生菌广泛用于人和动物.本文综述了乳酸杆菌改善宿主健康的机制.乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道.文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制. 相似文献
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Billinghurst RC Brama PA van Weeren PR Knowlton MS McIlwraith CW 《American journal of veterinary research》2004,65(2):143-150
OBJECTIVE: To determine whether serum concentrations of biomarkers of skeletal metabolism can, in conjunction with radiographic evaluation, indicate severity of osteochondrosis in developing horses. ANIMALS: 43 Dutch Warmblood foals with varying severity of osteochondrosis. PROCEDURE: 24 foals were monitored for 5 months and 19 foals were monitored for 11 months. Monthly radiographs of femoropatellar-femorotibial and tibio-tarsal joints were graded for osteochondral abnormalities. Serial blood samples were assayed for 8 cartilage and bone biomarkers. At the end of the monitoring period, foals were examined for macroscopic osteochondrosis lesions. RESULTS: Temporal relationships were evident between certain serum biomarkers and osteochondrosis severity in foals during their first year. Biomarkers of collagen degradation (collagenase-generated neoepitopes of type-II collagen fragments, type-I and -II collagen fragments [COL2-3/4C(short)], and cross-linked telopeptide fragments of type-I collagen) and bone mineralization (osteocalcin) were positive indicators of osteochondrosis severity at 5 months of age. In foals with lesions at 11 months of age, osteochondrosis severity correlated negatively with COL2-3/4C(short) and osteocalcin and positively with C-propeptide of type-II procollagen (CPII), a collagen synthesis marker. Radiographic grading of osteochondrosis lesions significantly correlated with macroscopic osteochondrosis severity score at both ages and was strongest when combined with osteocalcin at 5 months and CPII at 11 months. CONCLUSIONS AND CLINICAL RELEVANCE: The ability of serum biomarkers to indicate osteochondrosis severity appears to depend on stage of disease and is strengthened with radiography. In older foals with more permanent lesions, osteochondrosis severity is significantly related to biomarker concentrations of decreased bone formation and increased cartilage synthesis. 相似文献