共查询到19条相似文献，搜索用时 375 毫秒
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为了探究新疆一枝蒿口服液的最佳制备工艺,采用水提醇沉法提取新疆一枝蒿中的总黄酮,经单因素、响应面试验获取最佳的制备工艺参数,并通过薄层层析与含量限度测定对新疆一枝蒿口服液建立初步的质量控制。结果表明：新疆一枝蒿最佳制备工艺为料液比1:14,煎煮时间为1.5 h,醇沉浓度为50%。供试品色谱中,在与对照品色谱相应的位置上,显相同颜色的斑点,含量限度测定黄酮含量范围在28.67～29.59 mg/mL,平均值为29.10 mg/mL,最低限度为23.28 mg/mL。 相似文献
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紫花苜蓿中芦丁的提取及抗菌活性研究 总被引：1，自引：0，他引：1
本研究通过正交试验优选了紫花苜蓿中芦丁的提取工艺,以510nm最大吸光度进行可见光谱检测,测定芦丁含量,并对两种供试菌进行了体外抑菌活性测定.结果表明,通过单因素试验确定的各因素影响能力大小为：料液比＞乙醇浓度＞超声提取时间,含量最高为10.04mg/g;大肠埃希氏杆菌的MIC为0.40mg/mL,金黄色葡萄球菌的MIC为0.70mg/mL. 相似文献
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为了研究不同提取溶剂对黑沙蒿提取物得率、主要活性成分含量及其对自由基DPPH清除的影响,试验设5个处理,每个处理3个重复,各处理按1∶15(g/mL)料液比分别加入蒸馏水、乙醇、乙酸乙酯、乙醚和丙酮,通过超声波辅助法提取黑沙蒿中的生物活性成分;以维生素C为阳性对照,采用DPPH法来测定黑沙蒿不同溶剂提取物的抗氧化活性。结果显示:①与其他提取剂的提取物相比,蒸馏水提取物的得率最高(P0.05),且其多糖和黄酮含量也最高(P0.01),而乙醚提取物中多酚的含量最高(P0.01)。②同种溶剂提取物不同浓度相比,水提取物浓度为1 mg/mL时,对DPPH清除率极显著高于浓度为0、0.2和0.4 mg/mL时的清除率(P0.01);乙醇提取物浓度为0.4和0.6 mg/mL、乙酸乙酯提取物浓度为1 mg/mL、乙醚提取物浓度为0.8 mg/mL、丙酮提取物浓度为0.6 mg/mL时,对DPPH的清除率均极显著高于浓度为0和0.2 mg/mL时的清除率(P0.01)。③与其他溶剂提取物相比,当提取物浓度为0.2 mg/mL时,无水乙醇提取物对DPPH的清除率极显著高于其他4种提取物对DPPH的清除率(P0.01);提取物浓度为0.4和0.6 mg/mL时,水提取物对DPPH的清除率显著低于其他4种提取物对DPPH的清除率(P0.01);浓度为0.8和1 mg/mL时,乙酸乙酯提取物对DPPH的清除率极显著高于其他4种提取物对DPPH的清除率(P0.01)。综上,黑沙蒿水提取物得率及多糖和黄酮含量最高,乙醚提取物中多酚含量最高,而无水乙醇和乙酸乙酯提取物抗氧化效果最好。 相似文献
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桑枝中的黄酮是一种药理活性广泛的物质,具有重要的药用价值。文章采用甲醇水浴法对秋季22个桑品种资源不同枝段的黄酮类化合物进行了测定。结果表明:不同品种间黄酮含量存在较大差异,含量最高的品种是伦教40,为0.958mg/g,其次为沙2x伦109,含量为0.865 mg/g;不同枝段群组间黄酮含量的变异数差异显著,其中,桑枝条的中、下段黄酮含量均高于上段,在p0.05水平,中、下枝段与上段达显著差异,下段中平均含量0.812 mg/g,中段含量0.794 mg/g,桑枝中黄酮具有由上至下的富集趋势。 相似文献
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《中兽医医药杂志》2019,(2)
目的:优选红芪总黄酮超声提取的最优工艺并探讨提取物的抗氧化活性。方法:以总黄酮的得率及提取物的抗氧化活性为指标评价工艺,在单因素实验的基础上,运用正交试验法考察提取时间、超声功率、溶剂浓度三因素对红芪总黄酮得率及抗氧化活性的影响;紫外分光光度法测定提取物中总黄酮的含量,DPPH、O_2~-·法和ABTS 3种方法测定提取物的抗氧化活性。结果:红芪总黄酮超声提取的最优工艺为:70%乙醇,超声功率170 W,提取20 min,此工艺条件下提取的总黄酮具有较好体外抗氧化活性,高浓度的黄酮抗氧化活性相当于0.5 mg/mL维生素C,总黄酮对DPPH、O_2~-·和ABTS+的IC50分别为3.336 mg/mL、3.988 mg/mL、4.786 mg/mL。结论:经验证此工艺稳定可行,提取物抗氧化活性较传统的热回流法强,且呈一定的量效关系,可作为工业化大生产的参考。 相似文献
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用索氏回流法正交提取唐古特白刺中的黄酮类化合物,用黄酮类化合物最大吸光度（吸收波长510 nm）对提取物进行可见光谱检测,测定黄酮类化合物的含量。以芦丁标准品做对照,对比选取最佳提取工艺;并对两种供试菌进行体外最小抑制浓度（MIC）测定。结果表明,乙醇浓度、乙醇用量、回流时间、超声功率、超声时间的不同对唐古特白刺中黄酮的提取均有影响。其最佳工艺参数为：乙醇浓度65%、乙醇体积250 mL（唐古特白刺10.0 g）、回流时间1 h、超声功率70%、超声时间40 min;对大肠埃希氏杆菌、金黄色葡萄球菌的MIC分别为0.35 mg/mL和0.50 mg/mL。 相似文献
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Yates AC Stewart AA Byron CR Pondenis HC Kaufmann KM Constable PD 《American journal of veterinary research》2006,67(12):1980-1986
OBJECTIVE: To determine the effects of sodium hyaluronate (HA) in combination with methylprednisolone acetate (MPA) on interleukin-1 (IL-1)-induced inflammation in equine articular cartilage pellets. Sample POPULATION: Chondrocytes collected from 7 horses euthanatized for problems unrelated to the musculoskeletal system. PROCEDURES: Chondrocyte pellets were treated with medium (negative control); medium containing IL-1 (positive control); or medium containing IL-1 with MPA only (0.05 or 0.5 mg/mL), HA only (0.2 or 2 mg/mL), or MPA (0.05 or 0.5 mg/mL) and HA (0.2 or 2 mg/mL) in combination. Proteoglycan (PG) synthesis was determined by incorporation of sulfur 35-labeled sodium sulfate into PGs. Glycosaminoglycan (GAG) content of the media and the pellets and total pellet DNA content were determined. RESULTS: Methylprednisolone acetate at 0.5 mg/mL caused an increase in PG synthesis, whereas HA had no effect alone. The combination of MPA, both 0.05 mg/mL and 0.5 mg/mL, with HA at 2 mg/mL increased PG synthesis, compared with IL-1-treated control. All treatment groups containing the high concentration of MPA (0.5 mg/mL) and the high concentration of HA (2.0 mg/mL) had pellets with increased GAG content. The addition of HA caused an increase in total GAG content in the media, regardless of MPA treatment. Cyclooxygenase-2 mRNA and aggrecan mRNA expression was significantly reduced with MPA treatment. Total pellet DNA content was unchanged by any treatment. CONCLUSIONS AND CLINICAL RELEVANCE: Our results indicate that MPA in combination with HA has beneficial effects on PG metabolism of IL-1-treated equine chondrocytes. 相似文献
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近年来三亚滨海旅游发展迅速,给近岸海洋环境带来了负面影响。为比较三亚旅游旺季,不同滨海旅游活动海域的水质状况,于2013年1—4月对亚龙湾不同滨海活动海域水体中的pH值、溶解氧、亚硝酸盐、磷酸盐、总磷和化学需氧量进行了测定和分析。结果表明,溶解氧含量为6.3~7.4 mg/L,其中,参照点游泳区生活区外围快艇游弋区(P0.05);亚硝酸盐含量为0.003 8~0.005 7 mL/L,其中,快艇游弋区游泳区生活区外围参照点,且快艇游弋区显著高于参照点(P0.05);磷酸盐和总磷含量分别为0.0075~0.012 7mL/L和0.011 4~0.062 7 mL/L,其中,快艇游弋区生活区外围游泳区参照点,且快艇游弋区显著高于游泳区和参照点(P0.05);化学需氧量含量为0.48~1.28 mL/L,其中,生活区外围快艇游弋区游泳区参照点(P0.05)。结论:在旅游旺季,亚龙湾海域水体水质整体上符合Ⅰ类海水水质标准,但是各种滨海旅游活动对其近岸水体水质产生了不同程度的影响,使得旅游旺季某些海域水体在个别月份达到了Ⅱ类水质标准。对近岸水体水质影响最大的是快艇游弋活动,其次为沿岸酒店和宾馆的住宿活动。 相似文献
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本试验旨在研究饲粮中添加碘酸钙对牦牛瘤胃体外发酵的影响。以碘酸钙作为添加形式,以牦牛饲粮为底物进行体外瘤胃发酵,底物碘含量分别为0.1、0.3、0.5、0.7、0.9 mg/kg,发酵48h,测定发酵后的产气量、瘤胃发酵指标及消化酶活力。结果表明:当底物碘含量为0.3mg/kg,NH_3-N浓度、微生物蛋白质(MCP)含量、脂肪酶(LPS)活力、胰蛋白酶(TYS)活力、纤维素酶(CLS)活力达到最大值,分别为14.267 mg/100mL、4.619g/L、0.462U/mL、65.463U/mL、69.527U/mL,碘含量为0.3mg/kg时,乙酸(aceticacid)、丙酸(propionicacid)、总酸(TVFA)浓度达到最高值,分别为:56.142mmol/L、30.877mmol/L、87.460mmol/L,各组之间差异均不显著(P>0.05),乙酸与丙酸的比例达到最小值1.819,显著低于其它所有组乙酸与丙酸的比例(P<0.05);在底物碘含量为0.5mg/kg时,淀粉酶(AMS)活力与纤维素酶(CLS)活力达到最大值分别为1.832U/mL、69.527U/mL;在底物碘含量为0.7mg/kg时,总产气量与甲烷产量达到最大值分别为80.8mL、11.88mL。综合以上结果,在体外条件下,在底物碘含量为0.3～0.7mg/kg时,有利于瘤胃发酵和饲草料降解。 相似文献
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建立了烯丙孕素口服液中烯丙孕素含量测定的高效液相色谱分析方法及样品前处理方法。采用正相高效液相色谱法,前处理溶剂为异丙醇-正己烷(5:20),色谱柱为ZORBAX CN C18柱(250×4.0 mm,5μm),流动相正己烷-异丙醇(98:2),流速1.0 mL/min,柱温35℃,检测波长235 nm。该方法中烯丙孕素的定量限浓度为804.9 ng/mL,线性范围为0.401~0.601 mg/mL,平均回收率为100.3%,该方法前处理简单、准确度高,可适用于该制剂中烯丙孕素的定性定量测定。 相似文献
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Adin DB Hill RC Scott KC 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(5):724-726
Parenteral veterinary furosemide is a 50-mg/mL solution with a pH of 8.0-9.3. The purpose of this study was to determine whether a commonly used veterinary formulation of 50 mg/mL of furosemide solution could be diluted in vitro without precipitation. Furosemide 50 mg/mL was diluted to concentrations of 10 and 5 mg/mL with 5% dextrose in water (D5W), 0.9% saline, lactated Ringer solution (LRS), and sterile water. Acidic sterile water and basic sterile water solutions were made by the addition of hydrochloric acid and sodium hydroxide, respectively, for use as controls to assess the effect of pH extremes for each concentration. After furosemide dilution, the final pH of each sample was measured, and samples were grossly and microscopically examined for clarity and crystal formation immediately and 1, 3, 5, and 8 hours after dilution. Gross precipitation and microscopic crystals were immediately observed in the acidic controls. Solutions of 5 mg/mL in LRS and 0.9% saline became slightly cloudy immediately, but no crystals were observed microscopically for 8 hours. Solutions of 10 mg/mL in D5W, 0.9% saline, LRS, and sterile water and solutions of 5 mg/mL in D5W and sterile water and the basic control were grossly clear, and no microscopic crystals were observed for 8 hours. On the basis of the results obtained in this in vitro investigation, this veterinary formulation of furosemide 50 mg/mL can be diluted without precipitation to a concentration of 10 mg/mL with D5W, 0.9% saline, LRS, or sterile water and to 5 mg/mL with D5W or sterile water and held for 8 hours. 相似文献
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Doyle AJ Stewart AA Constable PD Eurell JA Freeman DE Griffon DJ 《American journal of veterinary research》2005,66(1):48-53
OBJECTIVE: To determine effects of sodium hyaluronate (HA) on corticosteroid-induced cartilage matrix catabolism in equine articular cartilage explants. SAMPLE POPULATION: 30 articular cartilage explants from fetlock joints of 5 adult horses without joint disease. PROCEDURE: Articular cartilage explants were treated with control medium or medium containing methylprednisolone acetate (MPA; 0.05, 0.5, or 5.0 mg/mL), HA (0.1, 1.0, or 1.5 mg/mL), or both. Proteoglycan (PG) synthesis was measured by incorporation of sulfur 35-labeled sodium sulphate into PGs, and PG degradation was measured by release of radiolabeled PGs into the medium. Total glycosaminoglycan (GAG) content in media and explants and total explant DNA were determined. RESULTS: Methylprednisolone acetate caused a decrease in PG synthesis, whereas HA had no effect. Only the combination of MPA at a concentration of 0.05 mg/mL and HA at a concentration of 1.0 mg/mL increased PG synthesis, compared with control explants. Methylprednisolone acetate increased degradation of newly synthesized PGs into the medium, compared with control explants, and HA alone had no effect. Hyaluronate had no effect on MPA-induced PG degradation and release into media. Neither MPA alone nor HA alone had an effect on total cartilage GAG content. Methylprednisolone acetate caused an increase in release of GAG into the medium at 48 and 72 hours after treatment. In combination, HA had no protective effect on MPA-induced GAG release into the medium. Total cartilage DNA content was not affected by treatments. CONCLUSIONS AND CLINICAL RELEVANCE: Our results indicate that HA addition has little effect on corticosteroid-induced cartilage matrix PG catabolism in articular cartilage explants. 相似文献
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试验为测定金荞麦超微粉中有效成分原花青素B2的含量,将四川金荞麦饮片粉碎至0.75 nm以下的超微粉,50%乙醇提取,并利用高效液相色谱测定原花青素B2的含量。结果显示,在0.0352~1.4080 μg/mL范围内原花青素B2与峰面积线性关系良好,回归方程为Y=59797X+592.3,r=0.996,金荞麦超微粉中原花青素B2含量在1.305~1.541 mg/g之间。本试验结果表明该方法能快速、稳定、精确的检测原花青素B2含量,为金荞麦制剂质量控制提供了科学依据。 相似文献
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建立高效提取桑叶植酸的方法,并应用于检测分析不同品种、不同成熟度桑叶中的植酸含量和蚕粪中的植酸含量,有助于进一步开展降低桑叶中的植酸含量及提高桑叶营养利用率的研究。运用Design-Expert 8.0.4 Trial软件的Box-Behnken模型,分析不同提取条件因子对桑叶植酸提取效果的影响作用为原料质量浓度>提取液中盐酸的浓度>浸提时间,确定最佳提取条件:提取液为1.25%盐酸+0.1 g/mL硫酸钠,浸提时间为2.18 h,原料质量浓度为0.05 g/mL。在此优化条件下提取桑叶和蚕粪中的植酸,并应用比色法测定5个桑品种春季成熟叶、嫩叶以及秋季成熟叶、嫩叶中的植酸平均质量比分别为3.98、3.55、3.75、3.52 mg/g,5龄幼虫蚕粪中的植酸含量为3.78 mg/g,与桑叶中的植酸含量接近。检测结果说明桑叶中的植酸随着桑叶的成熟其含量不断增加,家蚕不能吸收利用植酸态的磷。 相似文献