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广东地区鸭疫里氏杆菌的血清型及抗药性情况调查 总被引：4，自引：0，他引：4
对2006~2008年间从广东省规模化鸭场罹患鸭传染性浆膜炎的病鸭中分离的鸭疫里氏杆菌的血清型和抗药性流行情况进行了调查和分析。在122个鸭场中共分离鉴定出鸭疫里氏杆菌86株。平板凝集试验结果表明血清1型菌株有81株,占总分离株的94.18%;其他血清型有8型2株,4型、3型和10型各1株,提示血清1型仍是广东省流行的优势血清型。以纸片法测定所分离菌株对氟苯尼考等13种常用抗菌药物的敏感性,结果显示广东地区鸭疫里氏杆菌抗药性严重,其中仅对阿莫西林、氨苄青霉素、头孢噻吩和氟苯尼考相对敏感,敏感菌株的比例分别为82.55%、77.91%、82.55%和81.39%,对其他抗菌药物均有抗性,75.58%的菌株同时对5种以上的抗菌药物具有多重抗药性,且不同地区鸭疫里氏杆菌分离株的药物敏感性也存在明显差异。 相似文献
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为了解不同地区鸭疫里氏杆菌病的流行情况,并进一步测定这些地区鸭疫里氏杆菌对常用抗菌药物的敏感性。本试验从广东云浮、广东开平、浙江常山3个地区疑似传染性浆膜炎病鸭中进行了细菌分离,通过鉴别培养、染色镜检、生化试验等对分离菌进行鉴定。采用微量稀释法测定了分离菌株对常用药物的最小抑菌浓度。结果从3个地区共分离鉴定出16株鸭疫里氏杆菌。药敏试验结果表明,3个地区鸭疫里氏杆菌均对头孢类和氟苯尼考高度敏感,对多西环素、大观霉素比较敏感;对恩诺沙星,新霉素等其他受试药物呈不同程度的耐药。本研究为鸭疫里氏杆菌的分离鉴定与药物敏感性测定提供了系统方法,为临床选择合理药物防制鸭传染性浆膜炎提供了理论指导。 相似文献
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我国南方部分地区鸭疫里氏杆菌的分离鉴定及耐药情况调查 总被引：1，自引：0，他引：1
《畜牧与兽医》2019,(12):106-111
通过对我国南方地区患疑似浆膜炎的病鸭进行细菌分离纯化,对所得菌株革兰氏染色镜检,PCR鉴定及序列分析,最终分离到120株鸭疫里氏杆菌,并对菌株进行耐药检测。发现菌株对丁胺卡那、卡那霉素、链霉素、四环素、罗红霉素、红霉素、阿莫西林、青霉素、氨苄西林和环丙沙星有很强的耐药性,同时对头孢曲松、头孢吡肟、多西环素、左氟氧沙星和氟苯尼考呈现出较强的敏感性。通过对氨基糖苷类耐药基因的检测,发现在氨基糖苷类耐药菌株中存在大量的耐氨基糖苷类耐药基因。 相似文献
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本文旨在研究广东地区Ⅰ型鸭疫里氏杆菌流行分布情况及其分子分型特点。2007—2017年,收集广东地区病死鸭、鹅的脑或肝组织等样品,进行菌株的分离、纯化及PCR鉴定等试验;采用玻片凝集的方法对鸭疫里氏杆菌进行Ⅰ型血清型鉴定,并进一步对血清Ⅰ型菌株进行脉冲场凝胶电泳(PFGE)分型,用BioNumerics 6.0软件进行聚类分析,比较同源性。结果表明:2007—2017年本实验室共分离保存220株鸭疫里氏杆菌,其中有119株为Ⅰ型血清型,占总分离株的54.09%。PFGE分型得到33种不同的PFGE分型图谱(以相似度≥85%为判断标准),可分为19个簇及14个单一型。血清Ⅰ型是广东地区鸭疫里氏杆菌的优势血清型。Ⅰ型鸭疫里氏杆菌分子型别呈现多样化,菌株之间的同源性差异较高,但存在小范围的克隆传播。 相似文献
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为了对从南昌某疑似鸭疫里氏杆菌病的病死雏鸭大脑、肝脏中分离得到的1株菌株进行16S rDNA鉴定,并筛选敏感药物,试验采用染色镜检、PCR扩增、测序与序列比对分析及药敏纸片扩散法等对分离株进行研究。结果表明:经染色镜检,分离菌呈革兰氏染色阴性,菌体为单个或成双排列的小杆菌,无芽孢,将其命名为NC-RA1;PCR扩增得到大小约为1 500 bp的条带;分离株NC-RA1与鸭疫里氏杆菌16S rDNA基因同源性高达99%;分离株NC-RA1对氟苯尼考、替卡西林-克拉维酸、头孢噻肟敏感,对四环素中度敏感,对庆大霉素、卡那霉素等药物耐药。说明该分离株为鸭疫里氏杆菌,并且可以用氟苯尼考、替卡西林-克拉维酸、头孢噻肟对其进行防治。 相似文献
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血清5型鸭疫里氏杆菌在我国的发现及其病原特性研究 总被引：6，自引：0，他引：6
1994年1月至2000年10月，从全国25个省、自治区、直辖市的5－90日龄患典型鸭疫里氏杆菌病的种鸭和商品鸭中分离到89株血清5型鸭疫里氏杆菌。对其病原学特性研究结果表明，血清5型鸭疫里氏杆菌病在我国鸭群感染范围极广；分离菌株对雏鸭具有很强的致病性，对小白鼠无致病性；各地分离菌株表现出一致的形态特征和非常相似的生理生化特性；各地分离力株耐药谱很广，但对头孢类药物和利福平高度敏感。用分离菌株制备的灭活疫苗免疫雏鸭具有很好的免疫原性。 相似文献
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Beran GW 《Preventive veterinary medicine》2008,86(3-4):198-207
When early people made their appearance, zoonotic infectious diseases were already waiting, but epidemic diseases did not appear in human history until people began to live in large numbers under conditions of close contact, mainly during the last 10,000 years. Disease has decimated urban populations, conquered armies, and disrupted society. The focus here is on (1) the plague of Athens and the Black Death; (2) smallpox, influenza, and rabies; (3) avian influenza prion diseases, and foot & mouth disease; and (4) emerging and re-emerging diseases. All have veterinary public health associations. In Athens, Greece, in 430 BC, when the Spartans ravaged the countryside, hordes crowded into Athens so that orderly movements, space in which to live, and adequate supplies of food became impossible. Crowding of any population fosters disease transmission; chaos and disorder enhance it all the more. Out of northern Egypt came a terrible plague from across the Mediterranean Sea. The identity of the plague of Athens remains unsure, but the well-considered conclusion is Rift Valley Fever, a mosquito borne, viral zoonosis. The Black Death, also called the Plague, raged in Asia for centuries. In 1347, the Black Death was brought by a ship out of Asia to Sicily. The scenes of devastation were repeated throughout Europe, with 90% or more of the people dying in city after city. Influenza, too, has been a cause of periodic human epidemics, but the great pandemic of influenza occurred in the last months of World War I. In the years of highest occurrence, more than half the world's population became clinically infected. If veterinary public health had been born earlier, it could have led to elucidating the epidemiology of influenza and the plagues of Athens, Europe, and Asia. In turn, smallpox had also caused continual tragedy. In 1796, Edward Jenner began to harvest pustules of cowpox from children or infected cows and inject them into susceptible children. In 1980, the World Health Organization declared that smallpox had been eliminated from the world. Rabies, though, still strikes terror. A number of animal diseases, broadly termed emerging and re-emerging diseases, need surveillance because they have the potential to impact human health. From late in 2003 to 2007, the highly pathogenic H5N1 influenza virus in poultry infected at least 121 people and caused 62 deaths in four countries. The prion diseases, too, all have very high numbers in concentrated contacts. To control these diseases, veterinary public health is essential, with diagnosis, epidemiological surveillance, clinical manifestations, and prevention as primary measures. 相似文献
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Dekker A Dercksen D Snoep J van Wuyjckhuise L 《Tijdschrift voor diergeneeskunde》2007,132(18):695-701
On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures. 相似文献
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在动物的异常行为中以刻板行为最为常见,而咽气癖又是马最常见的口部刻板行为之一。作者就咽气癖在生理方面和心智方面对马体产生的影响、行为基础、诱因的研究进展及常见的防治措施进行综述。 相似文献
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近几年,世界饲用添加剂市场增长较快,预计今后将进一步增长,据世界粮农组织(FAO)首领会议的倡议,到2015年,将使世界营养不良的人口削减一半,其间,肉制品将会以每年2％的速度增长,特别是加快猪肉和禽肉增长。这将促进世界添加剂,尤其是蛋氨酸和赖氨酸需求量的增加。 相似文献
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