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1.
采集 30头四川白猪血样 ,采用聚丙烯酰胺凝胶电泳法 ,测定血清中运铁蛋白 (Tf)、前白蛋白(Pa)、后白蛋白 (Po)、血液结合素 (Hp)的多态型 ,估测其基因型频率和基因频率。测定结果表明 :四川白猪运铁蛋白有AA、AB、AC、BB、BC、CC六种表现型 ,前白蛋白有AA、AB、BB三种表现型 ,后白蛋白有AA、AB、BB三种表现型 ,血液结合素有 0 - 1、1 - 1、1 - 2、2 - 2、2 - 3五种表现型 ,其频率分别为 :0 0 6 6 7、0 2 0 0 0、0 2 6 6 7、0 2 0 0 0、0 2 333、0 0 333、0 4 333、0 4 6 6 7、0 1 0 0 0、0 5 333、0 30 0 0、0 1 6 6 7、0 30 0 0、0 4 6 6 7、0 0 333、0 1 0 0 0、0 1 0 0 0  相似文献   

2.
应用动物模型REML法估计金华猪繁殖性状遗传参数   总被引：9，自引：0，他引：9  
试验资料取自金华猪场 1 979～1 999年金华猪繁殖性能的生产记录。采用动物模型约束性最大似然法 (REML)对该猪群的遗传参数进行估计 ,测定 1 1 4 4头纯繁金华母猪的产仔哺育记录。结果表明 ,产仔数 (TNB)、产活仔数 (NBA)、初生重 (PBW)、初生窝重 (LBW)、 2 0日龄头数(NFC)、 2 0日龄窝重 (LWFC)、 2 0日龄重 (PWFC)、 60日龄头数 (NW)、 60日龄窝重 (LWW)、 60日龄重 (PWW)、乳头数 (TN)的遗传力分别为0 1 2± 0 0 9、 0 1 8± 0 1 1、 0 1 5± 0 0 9、 0 0 2± 0 0 9、 0 1 0± 0 1 1、 0 0 8± 0 1 3、 0 1 8± 0 1 0、 0 2 3± 0 1 1、 0 31± 0 1 3、 0 35± 0 1 1和 0 1 9± 0 1 0。TNB与NBA、LBW、NW、LWW的遗传相关范围为 0 66～ 0 88,TNB与PBW的遗传相关为 - 0 43。NBA与LBW、NW、LWW的遗传相关范围为 0 60～ 0 64,NBA与PBW遗传相关为 - 0 63。PBW与PWW的遗传相关为 0 51。  相似文献   

3.
生长肥育猪有效磷需要量的研究   总被引：5，自引：0，他引：5  
试验选用 1 2 0头体重约 2 0kg的三元杂交猪 [杜洛克× (约克夏×长白 ) ]分为 1 0组 ,采用 2 (一定的钙磷比 1 2 5∶1和一定的钙 (Ca)水平 :生长期 0 60 % ,肥育期 0 5 0 % )× 5 (有效磷 (AP)水平生长期0 1 3、0 1 8、0 2 3、0 2 8、0 3 3 % ,肥育期 0 0 7、0 1 1、0 1 5、0 1 9、0 2 3 % )随机组合设计 ,研究AP水平对 2 0～90kg生长肥育猪生产性能、骨骼发育和血清生化指标的影响。结果表明 ,(1 )饲粮钙磷比保持 1 2 5 :1时 ,随着AP水平的升高 :A、生长猪的平均日增重 (P >0 0 5 )、采食量 (P <0 0 1 )、血清无机磷浓度 (P <0 0 1 )提高 ,AP0 3 3 %～Ca0 72 %组料重比最低 ,各组血清钙浓度和碱性磷酸酶 (ALP)活性无显著差异 (P>0 0 5 ) ;B、肥育猪平均日增重趋于增加 (P >0 0 5 ) ,采食量极显著提高 (P <0 0 1 ) ,料重比AP0 1 1～0 1 9%组显著低于 0 0 7%和 0 2 3 %组 (P <0 0 5 ) ,血清中无机磷 (P >0 0 5 )和钙浓度 (P <0 0 1 )提高 ,无机磷浓度峰值在AP0 1 9%组出现 ,血清ALP活性AP0 0 7%～Ca0 3 9%组极显著或显著高于其它组 (P <0 0 1、0 0 5 ) ;C、股骨和第 3、4跖骨的长度、骨壁厚度、脱脂干骨重及其灰分、磷、钙的绝对重量随AP水平提高而显著或极显著 (P <  相似文献   

4.
在 3头装有永久性瘤胃瘘管成年梅花公鹿上 ,探讨了尼龙袋法测定钙、磷矿物质饲料的生物学效价。结果表明 :尼龙袋法能很好测定梅花鹿钙、磷矿物质的生物学效价 ;7种钙源饲料磷酸氢钙、硫酸钙、碳酸钙、石粉、骨粉、贝壳粉、鸡蛋壳粉的生物学效价 (% )分别为1 0 9 0 0 ,1 0 0 0 0 ,88 50 ,87 0 0 ,79 0 0 ,54 1 0和 2 6 60 ;4种磷源饲料磷酸氢钠、磷酸二氢钠、磷酸氢钙、骨粉的生物学效价 (% )分别为 1 0 0 0 0 ,1 0 0 0 0 ,81 35和 59 1 5。  相似文献   

5.
CT2000、T101在集约化鸡场的应用及其对蛋壳品质的影响   总被引：3，自引：2，他引：1  
1 50 0只 455日龄伊莎褐蛋鸡 ,随机分为对照组、CT2 0 0 0组和T1 0 1组 3组。各组日粮处理分别为 :对照组为基础日粮 ;CT2 0 0 0组为基础日粮 +40 0mg/kgCT2 0 0 0 ;T1 0 1组为基础日粮 +1 0mg/kgT1 0 1。试验为期 9周。结果表明 :与对照组比较 ,CT2 0 0 0、T1 0 1可显著提高产蛋后期蛋鸡生产性能 ;CT2 0 0 0组蛋壳厚度平均增加 0 0 0 3cm (P <0 0 1 ) ,蛋壳强度提高 0 0 4 1kg/cm2 (P <0 0 1 ) ;T1 0 1组蛋壳厚度平均增加 0 0 0 2cm (P <0 0 1 ) ,蛋壳强度提高 0 0 9kg/cm2 ,但各组蛋形指数无显著变化 (P >0 0 5)。结论 :CT2 0 0 0与T1 0 1均可以改善蛋壳品质 ,但CT2 0 0 0效果更好  相似文献   

6.
上市肉鸡组织样品中铅、镉、铜、铬含量的测定   总被引：4，自引：1，他引：4  
从江苏省某市采集上市肉鸡组织样品 ,采用微波消解 石墨炉 原子吸收分光光度法 ,测定主要组织 (心脏、肝脏、肌胃、胸肌、肾脏、腿肌、骨骼 )中铅、镉、铜、铬等重金属元素的含量。检验结果 :组织中Pb的含量以心脏最高 (1 3478± 0 531 2 )mg/kg ,肝脏次之 (1 1 2 6 3±0 534 0 )mg/kg ,骨骼最低 (0 1 0 1 8± 0 0 0 6 6)mg/kg ,样品含Pb超标率按上述组织顺序分别为 :1 0 0 %、 96 87%、 96 87%、 87 50 %、1 0 0 %、 81 2 5 %和 0 ;组织中Cd的含量以肾脏最高 (0 0 63 0± 0 0 2 0 2 )mg/kg,骨骼次之 (0 0 55 9± 0 0 2 0 5)mg/kg ,样品含Cd超标率分别为 :6 2 5 %、 0、 3 1 3 %、 0、 0、 0和 0 ;组织中Cu的含量以心脏最高 (8 492 7± 1 90 1 2 )mg/kg,肝脏次之 (8 462 8± 1 666 4)mg/kg ,腿肌最低 (0 8373± 0 741 4 )mg/kg ,样品含Cu超标率分别为 :1 2 50 %、 1 5 63 %、 9 38%、 3 1 3 %、 0、 0和 0 ;组织中Cr的含量以肾脏最高(1 642 5± 1 0 1 0 0 )mg/kg ,肝脏次之 (1 642 5± 0 61 97)mg/kg ,样品含Cr超标率分别为 :50 0 0 %、 62 70 %、 9 38%、 3 1 3 %、 0、 0和 0。检测数据表明 ,鸡主要组织样品中Pb含量严重超标 ;肝脏、肾脏中Cr超标较严重 ,Cd和Cu也有不同程度?  相似文献   

7.
将传染性法氏囊病病毒 ( IBDV) JD1 、JD2 、NB变异毒株直接适应于鸡胚成纤维细胞上致弱。 3个毒株2 1代和 3 5～ 3 8代细胞毒分别连续回归鸡体 5代。结果表明 ,IBDV JD1 株 2 1～ 3 5代、JD2 株 3 7代、NB株 3 8代细胞毒对雏鸡已基本失去致病力 ,是毒力相对稳定的弱毒株。 IBDV JD1 、JD2 、NB变异株弱毒以 3 0 0 0TCID5 0 、50 0 0 TCID5 0 、1 0 0 0 0 TCID5 0 、1 50 0 0 TCID5 0 4个不同免疫剂量免疫 SPF鸡后的攻毒试验结果表明 ,JD1 、NB毒株的免疫效果明显优于 B87、Bursine3毒株疫苗 ,具有优良的免疫原性。JD1 、NB毒株的最佳免疫剂量分别为 50 0 0 TCID5 0 、3 0 0 0 TCID5 0 。抗体消长规律表明 ,JD1 株疫苗一次免疫后的有效保护期为 2 1 4d,最低有效中和抗体保护效价为 1∶ 4 0 71。  相似文献   

8.
选择检疫期中 2岁左右的猕猴 4 2只 ,随机分成 7个组 ,其中 1组作为对照组 (抗生素 ) ,其余在饲喂基础日粮的同时分别饲喂浓度为 1∶5 0 0、1∶75 0、1∶10 0 0的EM饮水和 0 2 %、0 4 %、0 6 %的益生素饮水 10 0mL。结果表明 :1∶10 0 0的EM和 0 4 %益生素可以大大降低检疫期猕猴的腹泻率。  相似文献   

9.
以“杜长嘉”杂交仔猪为对象 ,研究大麦型饲粮中添加NSP酶制剂对仔猪生长性能和消化率的影响。结果表明 :与对照组相比 ,添加NSP酶使仔猪日增重提高 1 1 .54% (P <0 .0 1 ) ,饲料转化率提高5.33% (P <0 .0 5) ,日采食量提高 5.4 0 % (P <0 .0 5)。添加NSP酶可显著提高各种营养物质的消化率 ,与对照组相比 ,干物质、粗蛋白、粗脂肪、粗纤维、粗灰分和无氮浸出物的表观消化率分别提高 5.56% (P <0 .0 1 )、4 .79% (P <0 .0 1 )、2 8.79% (P <0 .0 1 )、1 5.0 5% (P <0 .0 1 )、32 .0 5% (P <0 .0 1 )和 4 .2 4 % (P <0 .0 1 )。  相似文献   

10.
不同能量水平日粮对肉牛瘤胃微生物氨基酸组成的影响   总被引：7，自引：0，他引：7  
:选用 1 2头杂种黄牛研究了不同能量水平 ( 5 2 0、6 2 7、7 87MJ/kg)的日粮对存在于瘤胃液中的微生物 (liquid associatedmicroorganisms,LAM)和在瘤胃中与饲料颗粒紧密结合的微生物 (solid associatedmi croorganisms,SAM)的氨基酸组成的影响。日粮由玉米、棉籽饼和玉米秸秆组成。结果表明 :能量水平对LAM中的Leu(P <0 0 0 1 )、Phe(P <0 0 1 )、Lys(P <0 0 0 1 )、His(P <0 0 0 1 )、Arg(P <0 0 0 1 )、Asp(P <0 0 0 1 )和Gly(P <0 0 0 1 )等 7种氨基酸的含量影响显著 ;对SAM中的Thr(P <0 0 5)、Val(P <0 0 5)、Lys(P <0 0 5)和Met(P <0 0 5)等 4种氨基酸的含量影响显著。不同组分的瘤胃微生物中 ,有 6种氨基酸的组成显著不同。在LAM中Glu(P <0 0 5)、Ala(P <0 0 0 1 )和Cys(P <0 0 0 1 )的含量明显高于在SAM中的含量而Leu(P <0 0 5)、His(P <0 0 1 )和Pro(P <0 0 0 1 )的含量则相反。另外His(P <0 0 5)、Arg(P <0 0 1 )、和Asp(P <0 0 1 )等 3种氨基酸在LAM和SAM中的相对组成受日粮能量水平的影响。结论 :不同组分瘤胃微生物的氨基酸组成有所差异 ,日粮能量水平对瘤胃微生物的氨基酸组成有明显影响  相似文献   

11.
Background: Regulatory T cells (Treg) have been shown to suppress antitumor immunity and often are increased in humans and rodents with cancer. However, Tregs have not been well studied in dogs with cancer and it is not known if certain tumor types are associated with increased Tregs.
Hypothesis: We hypothesized that Treg percentages would be increased in dogs with cancer and that Treg percentages would be higher in dogs with certain types of cancer.
Animals: The percentages and numbers of Tregs and nonregulatory T cells and B cells were assessed in 34 dogs with cancer and 9 age-matched control dogs. Dogs evaluated included 14 dogs with sarcoma, 7 dogs with carcinoma, 7 dogs with lymphoma, and 6 dogs with mast cell tumor.
Methods: Numbers and percentages of Tregs, CD4+, and CD8+ T cells and B cells were determined using flow cytometry and compared between control dogs and dogs with cancer.
Results: The percentage of Tregs was significantly increased overall in dogs with cancer compared with control dogs. When tumor types were compared, Treg percentages were significantly increased in dogs with carcinoma. The Treg/CD8 T cell ratio was significantly higher in dogs with cancer compared with control dogs and was also significantly increased in 2 dogs with T-cell lymphoma.
Conclusions: Treg percentages in blood were increased in dogs with cancer, particularly in dogs with carcinoma. The Treg/CD8 ratio also identified tumor-specific abnormalities in dogs with cancer. These findings indicate that tumor-specific factors may affect Tregs in dogs.  相似文献   

12.
Serum alpha-fetoprotein (AFP) values were measured in hepatic diseased dogs with or without tumor and non-hepatic tumor bearing dogs by a sandwich ELISA using anti-dog AFP antiserum. Serum AFP values were less than 70 ng/ml in clinically healthy dogs. The values in dogs with hepatocellular carcinoma were higher than 1,400 ng/ml in 7 of 9 dogs, wherever those in two dogs with cholangiocarcinoma were in the normal range. Serum AFP values in hepatic diseased dogs without tumor were also high, however, the values were below 500 ng/ml in 90% of the dogs. In non-hepatic tumor dogs, serum AFP values were less than 500 ng/ml in 76% of the dogs. In the surgically removal cases with hepatocellular carcinoma, serum AFP values rapidly decreased. These results suggested that the sandwich ELISA using anti-dog AFP antiserum was an available method for diagnosis of hepatocellular carcinoma in dogs.  相似文献   

13.
Plasma taurine concentrations were determined in 76 dogs with dilated cardiomyopathy (DCM), 28 dogs with acquired valvular disease (AVD), and 47 normal (control) dogs. The data were collected at 2 referral centers. The Animal Medical Center, New York, NY (AMC), and the University of California, Davis (UCD), and the studies were conducted independently. Different anticoagulants (sodium citrate at AMC and lithium heparin at UCD) were used to collect the plasma samples. Paired analysis of samples showed a significant difference in plasma taurine concentrations, depending on the anticoagulant used. Consequently, results from each clinic were analyzed separately. Plasma taurine concentrations were significantly higher in dogs with AVD (median, 133 nmol/mL; range, 25 to 229 nmol/mL) than in control dogs (median, 63 nmol/mL; range 44 to 224 nmol/mL) and dogs with DCM (median, 72 nmol/mL; range, 1 to 247 nmol/mL) at AMC (P= .001). The number of dogs with AVD at UCD was too small to draw meaningful conclusions. At UCD, the median plasma taurine concentration was 98 nmol/mL (range, 28–169 nmol/mL) in dogs with AVD, 75 nmol/mL (range, 0.1–184 nmol/mL) in dogs with DCM, and 88 nmol/mL (range 52–180 nmol/mL) in control dogs. There were no significant differences in plasma taurine concentrations between dogs with DCM and the control dogs at either hospital. Congestive heart failure and administration of cardiac medication had no significant effect on plasma taurine concentrations. Plasma taurine concentration was low (<25 nmol/mL) in 17% (13/76) of the dogs with DCM. Seven of the 13 dogs with low plasma taurine concentrations were Cocker Spaniels or Golden Retrievers. It was concluded that most dogs with DCM do not have low plasma taurine concentrations. However, certain breeds or individual dogs may have low plasma taurine concentrations in association with DCM. Whether this association is causal or not is unknown. The significance of the high plasma taurine concentrations in dogs with AVD is also unknown.  相似文献   

14.
The mast cell secretagogues compound 48/80 and codeine phosphate were evaluated as potential positive controls for intradermal skin testing in dogs. Wheal responses to both agents were compared with responses to histamine and saline in 11 normal dogs, and were strong and not significantly different from histamine responses in nine dogs ( P < 0.01), and significantly weaker than histamine in two dogs ( P < 0.05). Wheal responses to compound 48/80 (1 mg mL−1) were evaluated in 82 suspected atopic dogs and were similar to histamine in 79 dogs and markedly weaker than histamine in three dogs. Of nine confirmed atopic dogs with weak responses to injected allergens, seven had strong responses to compound 48/80, and eight had strong responses to histamine. Compound 48/80 and codeine phosphate appear unreliable positive controls for skin testing in normal dogs. Compound 48/80 (1 mg mL−1) may be a reliable positive control in atopic dogs but is a poor indicator of skin reactivity to allergens.  相似文献   

15.
Plasma histamine concentrations (PHCs) were measured serially over 9 months or until death in 11 dogs with mast cell tumors (MCTs). Eight dogs had grossly visible disease and the other 3 dogs had microscopic disease. Initial PHCs in the dogs with gross disease were significantly higher than PHCs in healthy dogs (median, 0.73 ng/mL and 0.19 ng/mL respectively; P < .009), whereas initial PHCs in dogs with microscopic disease showed no difference from controls. Seven dogs subsequently had progressive increases in PHC, and developed hyperhistaminemia (median, 14.0 ng/mL; range, 5.11-30.1 ng/nL). These 7 dogs died from MCTs, and 1 had general weakness with rapid lysis of a large tumor burden after radiation therapy. PHCs of the other 4 dogs were less than 1 ng/mL during the study. These 4 dogs were still alive with adequate control of the tumor at the conclusion of the study. Four of the 11 dogs initially had gastrointestinal (G1) signs, which abated soon after administration of histamine-2 (H-2) blockers. No significant difference was found between PHCs in dogs with GI signs and those without GI signs (median, 0.86 ng/mL and 0.35 ng/mL. respectively). Thereafter, 7 dogs had serious GI complications for which H-2 blocker therapy was ineffective. PHCs in these 7 dogs were extremely high (median, 12.2 ng/mL; range, 3.42-30.1 ng/nL). Results of this study demonsrated that PHC was one factor related to disease progression, and indicated that marked hyperhistaminemia was associated with the GI signs refractory to H-2 blocker therapy in dogs with MCTs.  相似文献   

16.
Urinary alkaline phosphatase (AP) and 7-glutamyl transferase (GGT) levels were measured in normal dogs, dogs with chronic renal failure, and dogs with acute renal failure, as confirmed by renal histology. The median values for urinary AP were 5–8 iu/litre/mmol creatinine for normal dogs, 6–7 for dogs with chronic renal failure and 49-4 for dogs with acute renal failure. The median values for urinary GGT were 3–4 iu/litre/mmol creatinine for normal dogs, 4–9 for dogs with chronic renal failure and 9-6 for dogs with acute renal failure. The results suggest that urinary AP can be used as an indicator of acute renal damage in the dog. GGT was shown to be less useful. No clear correlations were found between urinary enzyme levels and the extent of morphological kidney damage.  相似文献   

17.
硝唑尼特治疗犬贾第虫病的研究   总被引：1，自引：0，他引：1  
选择试验犬8只,将其随机分为4个小组,每组2只.连续6 d对8只试验犬分剐进行粪检,确定无贾第虫感染后,用体外纯培养的犬贾第虫滋养体接种试验犬,然后每天采集试验犬新鲜粪便40 g,用硫酸锌漂浮法进行粪检,当检测犬贾第虫感染呈阳性时,分别用1、2、4 mg/kg体质量剂量的硝唑尼特时1、2、3组试验犬进行灌服治疗,第4组试验犬不用药作为对照.用药后,每天以同样的方法检测贾第虫包囊,并计数.结果表明,以2、4 mg/kg体质量给药的试验犬1 d后粪检结果转为阴性,以1 mg/kg体质量给药的试验犬4 d后粪检结果转为阴性.结果表明以2、4 mg/kg体质量剂量的硝唑尼特对犬贾第虫痛有很好的疗效.  相似文献   

18.
The purpose of this study was to determine the frequency of hypoxemia and pulmonary mineralization using 99mTc-methylene diphosphonate (99mTc-MDP) in dogs with pituitary-dependent hyperadrenocorticism (PDH). Twenty-one dogs with PDH were pro-spectively evaluated using thoracic radiography, arterial blood gas analysis, and bone phase and pulmonary perfusion scintigraphy (using 99mTc-macro-aggregated albumin [99mTc-MAA]). The radiographs and bone and perfusion studies were evaluated subjectively. An averaged quantitative count density ratio was calculated between the thorax and cranial thoraco-lumbar vertebrae from lateral thoracic 99mTc-MDP images. Thoracic: vertebral ratios were calculated using 99mTc-MDP studies from 21 control dogs. The thoracic: vertebral ratios were compared between the 2 groups (PDH and control). The mean age (±SD) of the 21 PDH dogs was 10.2 (±3) years, whereas the mean age of the control group was 9.8 (±3) years. Seven of the 21 dogs with PDH were hypoxemic (denned as an arterial partial pressure of oxygen [PaO2] <80 mm Hg) with an average PaO2 (±SD) of 62 (±15) mm Hg. Of the 7 hypoxemic dogs, 2 were found to have pulmonary mineralization based on bone scintigraphic images. Pulmonary perfusion abnormalities were not identified using 99mTc-MAA in any of the 21 PDH dogs. Six PDH dogs had an abnormal interstitial pulmonary pattern and 5 of these dogs were hypoxemic. The average quantitative thoracic: vertebral ratio was not significantly different between the PDH and control dogs (0.5 ± 0.4 versus 0.4 ± 0.1, P = .16). Causes of hypoxemia other than pulmonary thromboembolism should be considered in dogs with PDH. Pulmonary mineralization may contribute to hypoxemia in dogs with PDH.  相似文献   

19.
Therapy of Immune Mediated Thrombocytopenia   总被引：3，自引：1，他引：2  
Fifteen dogs with immune mediated thrombocytopenia (IMT) were studied retrospectively. All dogs had a thrombocyte count below 50,000/microliters when response to therapy was studied. Platelet counts greater than 50,000/microliters were present in all dogs within 2-9 days of initiating medical therapy. Eight dogs experienced a single episode of thrombocytopenia and seven dogs relapsed over the following 5 to 24 months. Clinical parameters from dogs that experienced a single episode of IMT were compared with data from dogs that relapsed to determine whether any information would identify dogs that were prone to relapse. Signalment, severity of thrombocytopenia, and time to achieve a platelet count above 50,000/microliters were found not to differ (P greater than 0.05) between these two groups. Five of the seven dogs with relapsing IMT were splenectomized after 2 to 4 episodes (mean, 2.8 +/- 0.8) of thrombocytopenia over 2 to 14 months. The postoperative progress of these five dogs was followed for 6 to 17 months. Platelet counts were sustained above 200,000/microliters in 4/5 after splenectomy and it was possible to discontinue medical therapy in these dogs. In comparison, the 2 relapsing IMT cases that were not splenectomized continued to require intermittent immunosuppressive therapy. We conclude that signalment and routine pretreatment laboratory test results are not useful in distinguishing dogs with relapsing IMT from those that will experience one episode of IMT. Seemingly, splenectomy is useful in the management of dogs with relapsing IMT.  相似文献   

20.
The aim of this study was to detect Leishmania infantum DNA by real-time PCR in urine from different groups of dogs with clinical leishmaniosis. Urine from 10 clinically healthy dogs and 43 dogs with clinical leishmaniosis diagnosed by positive serology and/or bone marrow PCR were studied. The group of 43 dogs with clinical leishmaniosis was divided into three subgroups: 13 dogs with renal insufficiency and proteinuria (urine protein-creatinine ratio greater than one), 13 dogs with only proteinuria, and 17 dogs with neither renal insufficiency nor proteinuria. The detection of Leishmania DNA was performed by light cycler real-time PCR using hybridization probes in each urine sample. Leishmania positive PCR was found in 47% (20/43) of the urine from leishmaniotic dogs, while all urine from clinically healthy dogs were negative. The percentages of positive Leishmania PCR were 85% (11/13) in dogs with renal insufficiency and proteinuria, 23% (3/13) in dogs with proteinuria and 35% (6/17) in dogs with neither renal insufficiency nor proteinuria. Dogs with renal insufficiency and proteinuria presented a statistical significant greater percentage of positive Leishmania PCR in urine when compared with the other subgroups (P<0.02). This study demonstrates the presence of Leishmania DNA in urine of dogs with leishmaniosis. Those dogs with severe renal damage present a greater number of Leishmania parasites in urine.  相似文献   

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