共查询到20条相似文献，搜索用时 109 毫秒
1.
2.
禽轮状病毒的分离鉴定及部分特性研究 总被引：5，自引：2，他引：3
从北京某自然腹泻的鸡场 中成功地分离到一伯禽轮状病毒，并经鸡胚单层肝细胞和ＭＡ１０４细胞进行了传代培养，对其生物学特性作了部分研究，从而为我国禽轮状毒的毒株鉴定、诊断及疫苗等系列研究提供了一定依据。 相似文献
3.
4.
Avian influenza - diagnosis 总被引：1，自引：0，他引：1
Alexander DJ 《Zoonoses and public health》2008,55(1):16-23
5.
6.
为确定禽脑脊髓炎(AE)、鸡痘(AP)二联活疫苗的安全性、最小免疫剂量及免疫效力,自制3批二联活疫苗进行研究。结果显示,鸡接种10倍剂量疫苗后精神、食欲及发育均正常,临床表现和剖检均无异常;AEV YBF02株的最小免疫剂量为100 EID50,鸡痘鹌鹑化弱毒株的最小免疫剂量为10 EID50;3批疫苗AEV YBF02株的病毒含量均≥103.2EID50/羽份,鸡痘鹌鹑化弱毒株的病毒含量均≥103.4EID50/羽份,攻毒后保护指数为89~100。试验表明,自制二联活疫苗安全、有效、质量可控,可用于预防禽脑脊髓炎和鸡痘。 相似文献
7.
8.
聚丙烯酰胺凝胶电泳法鉴定禽呼肠孤病毒 总被引：1，自引：0，他引：1
从感染禽呼肠孤病毒的细胞中,以SDS和酚提取病毒核酸,通过聚丙烯酰胺凝胶电泳(PAGE)法将分节段的核酸分开,经银染色显示,呈3-3-1-3分布,此法可用于禽呼肠孤病毒的快速检测、鉴定。同样方法未能使传染性法氏囊病毒核酸显示。 相似文献
9.
禽波氏杆菌病是由禽波氏杆菌引起的一种火鸡的传染病。本文综述了该病的病原特性,临床症状与病理变化,诊断方法,以及该病的治疗与预防。 相似文献
10.
鸡白血病病毒抗原ELISA试剂盒的研制和应用 总被引：5，自引：2，他引：3
以双抗体夹心酶联免疫吸附试验（ＤＡＳ－ＥＬＩＳＡ）为基本原理，成功地研制出鸡白血病病毒抗原ＥＬＩＳＡ试剂盒，该试剂盒与国外同类试剂盒相比，具备相同的特异性和敏感性，对ＲＡＶ－１纯化样品的最小检出量可达０．７８ｕｇ／ｍｌ，且敏感性高于直接补体结合试验。经初步应用发现，我国商品种鸡群ＡＬＶ阳性率为０．７－１４％，在部分ＳＰＦ鸡群中未检出阳性鸡。 相似文献
11.
12.
Recent outbreaks of avian influenza (AI) occurring in Europe, in the Americas, in Asia and in Africa have provided field evidence of how challenging the control of this infection can be, particularly in densely populated poultry areas or in areas where free-range rural village poultry and backyard flocks are present. In these areas, laboratory testing is mainly applied to trace viral circulation in a given area or in a susceptible population to implement an early warning system, rather than to diagnose the presence of the virus in a diseased flock or animal. This implies the use of rapid, sensitive and possibly cost-effective laboratory tests adaptable to very high throughputs. As a consequence, new diagnostic approaches and technologies have been increasingly developed and applied. Molecular biology and biotechnology are providing important and precious contributions to the field of AI diagnosis, making extremely rapid, specific and sensitive techniques available. However, the use of some of these technologies is still limited, due to their costs and to the requirement of advanced technical and scientific expertise. Therefore, more conventional and well-established techniques, should not be abandoned but rather reconsidered and improved or modified. 相似文献
13.
检测鸡传染性鼻气管炎病毒抗体的间接ELISA方法的研究 总被引：1，自引：1，他引：0
分别用绿猴肾细胞和鸡胚成纤维细胞殖鸡传染性鼻气管炎病毒NL7784株，提纯抗原建立了检测鸡传染性鼻气管炎病毒抗体的间接酶联免疫吸附试验（ELISA），试验的最佳反应条件为：抗原最佳稀释度为1：160，酶标抗体为1：1000稀释，待检血清为1：160稀释，包被液为pH9．0的碳酸盐缓冲兴，6％犊牛血清（CS）作封闭液，稀释液用含10％CS的Tween－20磷酸盐缓冲液，洗涤液用含0．5％Tween－20的磷酸盐缓冲液，抗原与抗体的最佳反应时间为30分钟，底物的反应时间为15分钟。与中和试验进行了比较，证明所产方法具有很好的特异性、稳定性、可重复性和较高的敏感性。 相似文献
14.
15.
Suppression of protein expression of three avian reovirus S-class genome segments by RNA interference 总被引：1，自引：0，他引：1
RNA interference was used to suppress protein expression of three S-class genome segments of avian reovirus (ARV). Viral progeny titer was successfully down-regulated by RNA interference. Suppression of S1 genome segment, which has three open reading frames, not only decreased the expression level of the structural protein σC but also reduced cell fusion and the level of Ser15-phosphorylated p53 protein caused by the nonstructural proteins p10 and p17, respectively. Suppression of S2 or S4 genome segment by RNA interference could also reduce the expression level of σA or σNS. Interestingly, suppression of σNS resulted in down regulation of the expression of other viral products. In terms of variability of different genes among viral strains and of the impact after their suppression, it seems that the viral products involved in construction of viroplasm or core particles, like σNS, are considerable choices to efficiently inhibit ARV multiplication by RNA interference. Using a GFP reporter system, it was discovered that ARV could not inhibit activated RNA interference, suggesting that RNA interference may be used in the suppression of ARV infection. 相似文献
16.
禽流感病毒神经氨酸酶的结构及其生物学功能 总被引：2，自引：2，他引：0
高致病性禽流感是由禽流感病毒(AIV)引起的一种急性传染病。AIV呈球形,有囊膜。其表面主要有2种糖蛋白,即血凝素和神经氨酸酶。其中神经氨酸酶具有重要功能,其对病毒的释放及病毒在感染细胞周围的扩散能力有很大影响。此外,神经氨酸酶对其周围的血凝素切割能力也有很大影响,从而在一定程度上可以导致病毒致病性的不同。神经氨酸酶是AIV中另一种重要抗原,抗神经氨酸酶的抗体可为机体遭受流感病毒攻击提供一定的保护力。因此,神经氨酸酶对AIV的生物学特性具有重要意义。 相似文献
17.
18.
Avian influenza (AI) is a listed disease of the World Organisation for Animal Health (OIE) that has become a disease of great importance both for animal and human health. Until recent times, AI was considered a disease of birds with zoonotic implications of limited significance. The emergence and spread of the Asian lineage highly pathogenic AI H5N1 virus has dramatically changed this perspective; not only has it been responsible of the death or culling of millions of birds, but this virus has also been able to infect a variety of non-avian hosts including human beings. The implications of such a panzootic reflect themselves in animal health issues, notably in the reduction of a protein source for developing countries and in the management of the pandemic potential. Retrospective studies have shown that avian progenitors play an important role in the generation of pandemic viruses for humans, and therefore these infections in the avian reservoir should be subjected to control measures aiming at eradication of the Asian H5N1 virus from all sectors rather than just eliminating or reducing the impact of the disease in poultry. Collection and analysis of information in a transparent environment and close collaboration between the medical and veterinary scientific community are crucial to support the global AI crisis. 相似文献
19.
禽流感病毒分离株A/Goose/Guangdong/3/96(H5N1)HA基因序列分析 总被引：20，自引：4，他引：16
采用RT_PCR技术,以A/Goose/Guangdong/3/96(H5N1)RNA为模板,扩增了1.73kb 的HA 全基因cDNA。将HAcDNA克隆后进行了序列测定,测序结果表明所扩增的1728 个核苷酸片段包含了完整的HA基因的开放阅读框架和上下游引物序列、蛋白质合成的起始密码子和终止密码子。核苷酸序列比较分析结果表明:A/Goose/Guangdong/3/96(H5N1) 与A/Goose/Guangdong/1/96(H5N1)有11 个核苷酸差异,同源率99.4% ;与A/HongKong/156/97(H5N1) 有25 个核苷酸差异,同源率98.6 % ;与A/Chicken/HongKong/258/97 (H5N1) 有30 个核苷酸差异,同源率98.3% ;它们的氨基酸序列同源率依次分别为99 .2 % 、98.6% 和98.1% 。受体结合位点的氨基酸序列完全一致;HA裂解位点氨基酸序列也完全一致,各有5 个碱性氨基酸插入。这说明上述4 个流感病毒分离株可能来自同一个祖先,具有相同的毒力和相似的生物学特性。 相似文献