首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 93 毫秒
1.
建立了一种可准确定性定量检测4种牛可食性组织中吡利霉素残留的液相色谱-三重四极杆/线性离子阱(LC-Q-trap MS)复合质谱分析技术。牛肌肉、脂肪、肾脏和肝脏样品经5%甲酸乙腈提取,captiva EMR-Lipid柱净化。以0.1%甲酸水溶液和0.1%甲酸乙腈溶液为流动相,经Kinetex F5色谱柱分离后,用多反应监测联合信息依赖性采集与增强子离子扫描(MRM-IDA-EPI)模式检测,基质匹配标准溶液校正,外标法定量。结果表明:吡利霉素于牛肌肉和脂肪中在1～100 ng/mL的浓度范围内,于牛肾脏和肝脏中在1～400 ng/mL浓度范围内均呈现良好的线性关系,相关系数(R~2)均大于0.990;在4种空白组织中吡利霉素的检测限均为5μg/kg,定量限均为10μg/kg。吡利霉素在牛肌肉和牛脂肪10～200μg/kg、牛肾脏10～800μg/kg,以及牛肝脏10～2000μg/kg添加浓度范围内的回收率范围在60.2%～101%之间;批内与批间相对标准偏差均小于13%。该方法具有简便快速、灵敏度高、定性准确,重复性好等特点,可以满足上述组织中吡利霉素残留检测的要求。  相似文献   

2.
建立超高效液相色谱-串联质谱法测定猪牛组织中甲基盐霉素残留量。猪、牛组织中残留的甲基盐霉素,经乙腈溶液提取,硅胶固相萃取柱净化,超高效液相色谱-串联质谱法测定,外标法定量。甲基盐霉素在5 ng/mL～250 ng/mL的浓度范围内呈现良好的线性关系,甲基盐霉素在猪、牛肌肉、肝脏、肾脏、脂肪中的检测限均为2.5μg/kg,定量限均为5.0μg/kg,猪牛肌肉、肝脏、肾脏、脂肪中5.0 ng/g～100 ng/g添加浓度范围内的回收率均值为71.9%～88.4%,批内批间RSD值均<15%。  相似文献   

3.
建立了一种检测牛可食性组织中氟佐隆残留的超高效液相色谱-串联质谱法。牛肌肉、肝脏、肾脏和脂肪样品经90%乙腈溶液提取，高速离心去除蛋白质等杂质，EMR-Lipid柱净化。以0.1%甲酸乙腈溶液和0.1%甲酸水溶液为流动相，在C18色谱柱上分离。在电喷雾正离子(ESI+)模式下采用多反应监测模式(MRM)检测，基质匹配标准溶液外标法定量。结果表明：氟佐隆对牛肌肉在1～100 ng/mL浓度范围内，对牛肝脏、肾脏和脂肪在1～500 ng/mL浓度范围内均呈现良好的线性关系，决定系数(R2)均大于0.990;在4种牛可食性组织中氟佐隆的检测限均为5μg/kg,定量限均为10μg/kg。氟佐隆在牛肌肉10～400μg/kg、牛肝脏和牛肾脏10～1000μg/kg、牛脂肪10～14000μg/kg空白添加浓度范围内的回收率为72.0%～98.6%;批内与批间相对标准偏差均小于15%。该方法具有简便快捷、选择性好、定性准确，重复性好等特点，可以满足上述组织中氟佐隆残留检测的要求。  相似文献   

4.
建立了一种可准确定性定量检测4种牛可食性组织中莫昔克丁残留的液相色谱-三重四极杆/线性离子阱(LC-Qtrap)复合质谱分析技术。牛肌肉、肝脏、肾脏和脂肪样品经乙腈提取,高速离心去除蛋白质等杂质,C18柱净化。以0.1%甲酸水溶液和0.1%甲酸乙腈溶液为流动相进行洗脱,在BEH C18色谱柱上实现分离,在电喷雾正离子(ESI+)模式下,用多反应监测联合信息依赖性采集与增强子离子扫描(MRM-IDA-EPI)模式检测,基质匹配标准溶液外标法定量。结果表明:莫昔克丁在牛肌肉和牛肾脏1~100 ng/mL的基质匹配浓度范围内;在牛肝脏1~200 ng/mL的基质匹配浓度范围内和在牛脂肪1~1000 ng/mL的基质匹配浓度范围内均呈现良好的线性关系,相关系数(R 2)均大于0.990;在4种牛可食性组织中莫昔克丁的定量限均为2μg/kg。莫昔克丁在牛肌肉2~40μg/kg、牛肝脏2~200μg/kg、牛肾脏2~100μg/kg和牛脂肪2~1000μg/kg添加浓度水平上的回收率范围为65.6%~115%;批内与批间相对标准偏差均小于15%。该方法具有简便快速、灵敏度高、定性准确,重复性好等特点,可以满足上述组织中莫昔克丁残留检测的要求。  相似文献   

5.
建立高效液相色谱荧光检测法测定牛羊组织中碘醚柳胺残留量。牛羊组织中残留的碘醚柳胺,经乙腈-丙酮溶液提取,阴离子交换固相萃取柱净化,高效液相色谱-荧光法测定,外标法定量。碘醚柳胺在10～5000 ng/mL的浓度范围内呈现良好的线性关系,碘醚柳胺在牛羊肌肉、肝脏、肾脏、脂肪中的检测限均为2.5 μg/kg,定量限均为5 μg/kg,牛羊肌肉、肝脏、肾脏、脂肪中15～500 ng/g添加浓度范围内的回收率为77.4%～93.8%,批内批间RSD值均<15％。  相似文献   

6.
建立了一种检测牛组织和牛奶中咪多卡残留检测的高效液相色谱-串联质谱法。牛组织(肌肉、肝脏、肾脏、脂肪)和奶在NaAc缓冲体系中酶解,经HCl溶液提取,WCX固相萃取柱净化,以0.3 %甲酸水溶液(含20mM甲酸铵)和0.3 %甲酸乙腈为流动相进行梯度洗脱,在HILIC色谱柱上分离,在电喷雾正离子(ESI+)模式下,用多反应监测(MRM)模式检测,同位素内标法定量。结果表明：咪多卡在2.5～1000 ng/mL的浓度范围内呈现良好线性关系,相关系数(R2)大于0.99;咪多卡在牛组织和奶中的检测限均为10 μg/kg,定量限均为20 μg/kg;咪多卡在牛组织和奶中20～4000 μg/kg添加浓度水平上的回收率在70.9～109%范围内;批内RSD在0.55~9.59%之间,批间RSD在2.21~12.1%之间。该方法具有灵敏度高、定量准确,重复性好等特点,可以满足牛组织和奶中咪多卡残留检测的要求。  相似文献   

7.
为了建立一种可靠、灵敏的检测猪可食性组织中加米霉素残留量的高效液相色谱-串联质谱方法,本试验将猪组织(肌肉、肝脏、脂肪和肾脏)经绞碎、均质后,用乙腈提取,乙腈饱和正己烷去脂,固相萃取柱净化,Kinetex C18色谱柱梯度洗脱,在电喷雾正离子下以多反应监测模式检测,内标法定量。结果显示,该方法的检测限和定量限分别为3μg/kg和5μg/kg;加米霉素在5～1 000μg/kg浓度范围内,峰面积与浓度的线性关系良好;猪肌肉、肝脏和脂肪在5、50、100μg/kg和200μg/kg四个添加浓度的平均回收率为70.20%～90.75%,批间变异系数≤11.94%;猪肾脏在5、150、300μg/kg和600μg/kg四个添加浓度的平均回收率为66.69%～87.46%,批间变异系数≤9.95%。结果表明该方法灵敏、准确,可满足兽药残留检测的要求。  相似文献   

8.
陆春波 《中国兽药杂志》2012,46(8):24-27,38
建立了牛组织中氨丙啉残留检测的高效液相色谱法.试样中的氨丙啉经乙腈提取后旋转蒸干,用磷酸盐缓冲液溶解,过HLB固相萃取小柱净化,高效液相色谱法进行测定.结果表明,在0.20～20.0 μg/mL范围内,氨丙啉浓度与峰面积呈良好线性关系(r2=0.9999);在牛的肌肉、肝脏、肾脏和脂肪中,氨丙啉的检测限为100 μg/kg,在牛的肌肉、肝脏、肾脏中定量限为250 μg/kg,在牛的脂肪中定量限为200 μg/kg.在不同添加浓度下测定,氨丙啉的平均回收率为60％ ～ 110％,批内和批间变异系数均在15％以内.本方法可快速测定牛组织中氨丙啉残留量,且操作简单、灵敏度高、结果准确性高,能满足日常检测需要.  相似文献   

9.
为了测定牛肌肉、肝脏、肾脏组织中双氯芬酸钠的残留量,研究建立了一种用于该检测的高效液相色谱-串联质谱(LC-MS/MS)方法。试验将样品中加入乙腈提取液重复提取2次,超声波辅助提取,提取液中加入C18、PSA混合吸附剂进行净化。色谱柱采用C18反相柱对药物进行分离,洗脱液采用乙腈-0.1%甲酸水进行等度洗脱,配备电喷雾离子源,药物在正离子模式下电离,多反应监测模式(MRM)分段扫描测定检测结果,并采用内标法定量分析。结果表明,双氯芬酸在0.5～100μg/L浓度范围内有良好的线性关系,相关系数大于0.99;在牛的肌肉、肝脏和肾脏组织中检测限(LOD,S/N=3)为0.5μg/kg,定量限(LOQ,S/N=10)为1.0μg/kg。在3个浓度添加水平下,平均回收率为97.7%～114%,日内变异系数0.5%～4.7%,日间变异系数为0.9%～7.5%。该方法操作简便、结果准确,满足兽药残留检测方法规定要求,运用于快速准确测定牛肌肉、肝脏、肾脏等组织中双氯芬酸钠的残留。  相似文献   

10.
为建立一种高效液相色谱-串联质谱法检测鸡蛋和鸡组织(肝脏、肾脏、肌肉、脂肪)中恩诺沙星、环丙沙星的残留,鸡蛋和鸡组织(肝脏、肾脏、肌肉、脂肪)经酸化乙腈提取,正己烷除脂净化,以0.1%甲酸水溶液和乙腈为流动相进行梯度洗脱,同位素内标法定量。结果显示:恩诺沙星、环丙沙星均在线性范围内呈现良好线性关系,相关系数(R2)均大于0.99;恩诺沙星、环丙沙星在组织中的检测限为1μg/kg,定量限为5μg/kg;两种药物的平均回收率范围为75.5%～106%,批内、批间变异系数均小于11.2%。研究表明,该方法样品前处理简单,灵敏度高,适用同时检测鸡组织(肝脏、肾脏、肌肉、脂肪)和鸡蛋中恩诺沙星、环丙沙星残留。  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
Tropical Animal Health and Production -  相似文献   

14.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

15.
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.  相似文献   

16.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

17.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

18.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

19.
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors.  相似文献   

20.
<正>1.INTRODUCTION Ensuring transportation network security is one Of the most daunting challenges confronting homeland security agencies today.Significant research has been dedicated.To model and analyze the vulnerability of transportation systems,while notably fewer studies propose specific strategies for deploying defensive technologies to safeguard these systems.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号