共查询到20条相似文献，搜索用时 62 毫秒
1.
研究了基本培养基、碳源、营养添加物、pH值、固化剂等几种因素对日本结缕草‘Qingdao’和‘Zenith’两个品种成熟种子为外植体诱导愈伤组织的影响。结果表明:蔗糖作为碳源、pH值5.4～6.0、以植物凝胶或脱乙酰吉兰糖作为固化剂有利于日本结缕草愈伤的诱导与生长,NMB培养基、NB培养基分别有利于Zenith及Qingdao胚性愈伤的形成;而营养添加物的加入虽可提高愈伤诱导率,却导致胚性愈伤率的下降。同时,根据愈伤组织生长曲线得出,愈伤组织最适继代周期为24～33天。 相似文献
2.
结缕草愈伤组织诱导的营养因素优化 总被引：18，自引：0，他引：18
试验以优化设计结缕草成熟胚愈伤组织诱导的营养因素为目的,采用L9(34)的正交设计,优化选择结缕草成熟胚性愈伤组织诱导的培养基类型、糖类型及浓度、酶水解酪蛋白浓度等营养因素.试验表明:①不同营养因素组合的各处理对出愈率和出愈时间的影响不具显著差异.②培养基类型和酶水解酪蛋白的含量对愈伤组织大小的影响具有显著的效应.③培养基类型、酶水解酪蛋白相对于糖浓度和糖类型对胚性愈伤组织的形成具有显著作用.试验结论:MS培养基、糖类型S+G、糖浓度40g/L、酶水解酪蛋白1 g/L,为结缕草愈伤组织诱导的最佳营养因素组合. 相似文献
3.
结缕草‘Zenith’离体培养植株再生体系优化研究 总被引：2，自引：2，他引：0
以结缕草Zoysia japonica栽培品种‘Zenith’的成熟种子为外植体,通过调整2,4 D浓度和凝固剂种类及其浓度,进行结缕草离体培养植株再生体系优化的研究。结果表明：愈伤组织诱导的适宜培养基为MS+2,4 D 4.0 mg/L,愈伤组织诱导率为69.97%,其中胚性愈伤组织诱导率达28.78%。胚性愈伤组织包括2种类型：淡黄色、湿润的小颗粒聚集状愈伤组织(27.36%)和黄色、干燥、颗粒状愈伤组织(1.42%)。以结冷胶为凝固剂,有利于提高愈伤组织的诱导率。提高愈伤组织继代培养基中的琼脂浓度,有利于保持胚性愈伤组织的植株再生能力。适宜的愈伤组织分化培养基为MS+KT 4.0 mg/L或MS+6 BA 3.0 mg/L,绿苗分化率达90%以上。适宜的生根培养基为1/2MS ,生根率100%。 相似文献
4.
本试验以3个结缕草（Zoysia spp.）（品）种（兰引3号,马尼拉,青岛）的茎基作为外植体材料,诱导愈伤组织,观察出愈率,然后用不同剂量（5~40 Gy）的60Co γ射线辐照处理愈伤组织,观察其胚性愈伤组织诱导情况。结果表明,较低剂量（15～20 Gy）的辐照处理可以促进结缕草愈伤组织向胚性愈伤组织转化,在这一剂量范围内,3个结缕草（品）种不但可以获得很高的出愈率,同时也可获得较高的胚性愈伤组织形成率。胚性愈伤组织诱导的临界剂量是20 Gy, 此时,兰引3号、马尼拉和青岛愈伤组织的胚性转化率均达到了峰值,分别为76.47%、63.64%和37.5%。3个结缕草品种愈伤组织辐照的半致死剂量为30 Gy。 相似文献
5.
松南结缕草成熟胚愈伤组织的诱导和再生 总被引：2，自引：1，他引：1
松南结缕草成熟种子经灭菌和打破休眠后,接种于含有不同浓度2,4-D的MS和N6培养基上进行愈伤组织诱导。结果表明,结缕草种子的休眠主要在于破除种壳。2,4-D浓度在2~5 mg/L对愈伤组织诱导率无明显影响。基本培养基MS和N6诱导效果相近,初生愈伤组织为白色、半透明,质地松软、水浸状。继代培养基中添加一定浓度的甘露醇、麦芽糖和ABA以及延长继代时间有助于愈伤组织的胚性化。基本培养基MS更适于结缕草初生愈伤组织致密胚性化。胚性愈伤组织在MS添加0.1 mg/L 2,4-D培养基上即可分化出大量具有根和茎叶的再生植株。 相似文献
6.
日本结缕草植株再生体系的研究 总被引：1，自引：3，他引：1
以日本结缕草的成熟胚为外植体,对其组织培养体系进行了优化.试验结果表明,1)培养基中添加脯氨酸能提高愈伤组织诱导率,而添加BA能降低愈伤组织的诱导率,低浓度的NAA(0.1～0.2 mg/L)能促进愈伤组织的形成,愈伤组织诱导的最佳培养基为ND 2,4-D 3.0 mg/L NAA 0.2 mg/L L-Pro 500 mg/L,愈伤组织诱导率达57.3%;2)愈伤组织继代的最佳培养基为MSD 2,4-D 1.0 mg/L;3)愈伤组织分化的最佳培养基为MSD NAA 0.3 mg/L KT 0.5 mg/L BA 0.05 mg/L,分化率达36.7%. 相似文献
7.
以中华结缕草成熟胚为外植体,通过胚性愈伤组织的发生进行植株再生。结果表明,中华结缕草成熟胚在MS基本培养基附加6~7 mg/L 2,4-D和0.05 mg/L 6-BA的出愈能力很强,但初生愈伤组织呈无定型棉絮状,不能再生成株。采用不同的糖类和固化剂以及一定比例的生长素与细胞分裂素的方法,对初生愈伤组织进行继代培养,诱导出致密颗粒状的、具有高度再生能力的胚性愈伤组织,此类胚性愈伤组织在最佳分化培养基MS 1.0mg/L NAA 1.0 mg/L KT 3.00 mg/L 6-BA上有较高再生频率,生根培养基采用1/2 MS。 相似文献
8.
9.
以野生结缕草(Zoysia japonica)的成熟胚为外植体,通过胚性愈伤组织诱导进行植株再生。试验结果表明,不同的预处理方法对愈伤组织的诱导有较大的影响,经研钵研磨去除种子颖壳,30%NaOH浸泡1min,流水冲洗30 min处理的成熟胚在MS+2,4-D(3 mg/L)+6-BA(0.1 mg/L)为最佳,愈伤组织诱导率达82.67%。将愈伤组织在2,4-D(2 mg/L)和6-BA(0.2~0.5 mg/L)的继代培养基中继代1~2次,可明显改善愈伤组织状态,增加胚性愈伤数。筛选继代后的胚性愈伤组织置于分化培养基MS+KT(1 mg/L)+NAA(0.1 mg/L)中,分化率达86%。分化后的簇状植株移栽成活率达100%。 相似文献
10.
11.
12.
魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
13.
14.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
15.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
16.
17.
18.
Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献
19.
乳酸杆菌作为益生菌广泛用于人和动物.本文综述了乳酸杆菌改善宿主健康的机制.乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道.文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制. 相似文献
20.
Billinghurst RC Brama PA van Weeren PR Knowlton MS McIlwraith CW 《American journal of veterinary research》2004,65(2):143-150
OBJECTIVE: To determine whether serum concentrations of biomarkers of skeletal metabolism can, in conjunction with radiographic evaluation, indicate severity of osteochondrosis in developing horses. ANIMALS: 43 Dutch Warmblood foals with varying severity of osteochondrosis. PROCEDURE: 24 foals were monitored for 5 months and 19 foals were monitored for 11 months. Monthly radiographs of femoropatellar-femorotibial and tibio-tarsal joints were graded for osteochondral abnormalities. Serial blood samples were assayed for 8 cartilage and bone biomarkers. At the end of the monitoring period, foals were examined for macroscopic osteochondrosis lesions. RESULTS: Temporal relationships were evident between certain serum biomarkers and osteochondrosis severity in foals during their first year. Biomarkers of collagen degradation (collagenase-generated neoepitopes of type-II collagen fragments, type-I and -II collagen fragments [COL2-3/4C(short)], and cross-linked telopeptide fragments of type-I collagen) and bone mineralization (osteocalcin) were positive indicators of osteochondrosis severity at 5 months of age. In foals with lesions at 11 months of age, osteochondrosis severity correlated negatively with COL2-3/4C(short) and osteocalcin and positively with C-propeptide of type-II procollagen (CPII), a collagen synthesis marker. Radiographic grading of osteochondrosis lesions significantly correlated with macroscopic osteochondrosis severity score at both ages and was strongest when combined with osteocalcin at 5 months and CPII at 11 months. CONCLUSIONS AND CLINICAL RELEVANCE: The ability of serum biomarkers to indicate osteochondrosis severity appears to depend on stage of disease and is strengthened with radiography. In older foals with more permanent lesions, osteochondrosis severity is significantly related to biomarker concentrations of decreased bone formation and increased cartilage synthesis. 相似文献