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应用猪圆环病毒2型抗体酶联免疫检测试剂盒,测定了青海省省内8个猪场的1 000个血清样本,对不同地区、不同年龄猪的进行了血清学调查.结果显示,青海省8个猪场均有猪圆环病毒2型( PCV-2)感染,全省猪的PCV-2感染的平均血清阳性率为25.9％,其中母猪、种公猪、生长育肥猪、保育猪及哺乳仔猪抗体阳性率分别35.3％、... 相似文献
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2022年2月1日，陕西省某规模化母猪场产房仔猪断尾去势液检测猪繁殖与呼吸综合征病毒核酸阳性，该批仔猪于2022年2月23日断奶至下游育肥猪场，猪群断奶后3 d出现咳嗽、少食、发热等临床症状，根据临床症状、剖检大体病理变化、兽医实验室检测结果诊断为猪繁殖与呼吸综合征病毒和圆环病毒2型混合感染。为迅速控制猪群病情，对该猪场免疫程序及保健方案进行调整，并对猪群进行为期18周的猪繁殖与呼吸综合征和圆环病毒2型病毒血症期和抗体水平的持续跟踪发现，紧急防控方案实施后2周大群趋于稳定，猪繁殖与呼吸综合征疫苗二免后10周猪群的病毒血症消失，圆环病毒2型疫苗二免后猪群的病毒血症虽未消失，但阳性率有所下降，猪繁殖与呼吸综合征的抗体水平在猪群二免后8周趋于稳定，猪群受圆环病毒2型疫苗毒株及野毒的双重刺激，抗体衰减规律较为复杂，从持续的跟踪检测分析发现圆环病毒2型疫苗二免后6周抗体水平有所下降，8周后抗体水平上升并持续至该批猪出栏。 相似文献
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猪圆环病毒病是指以圆环病毒-2型（PCV2）为主要病原,单独或混合感染。它是断奶仔猪多系统衰竭综合征的主要病原,一般于断奶后2～3 d或1周发病,急性发病猪群中,病死率可达10%。大肠杆菌主要是一种条件性致病菌,其中以仔猪黄白痢最为常见。2016年3月份,某猪场1月龄仔猪突然发病,病死率较高,送检病猪3头进行病因诊断。为了解该规模化猪场疫病的感染状况,通过该猪场猪群发病情况及剖检病理观察,初步判断该猪场发病猪疑似为猪瘟病毒、猪圆环病毒2型或细菌感染,通过进一步分子生物学检测及细菌分离培养与鉴定,确诊该猪场发病猪为猪圆环病毒2型和大肠杆菌混合感染。 相似文献
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为了解淮安市规模猪场猪伪狂犬病毒与圆环病毒2型感染情况,应用ELISA试验对全市17个规模猪场共计349份血清样品进行集中监测,结果显示：全市规模猪场猪伪狂犬病毒与圆环病毒2型感染率分别为6.88%和72.49%,猪伪狂犬病毒与圆环病毒2型混合感染率为10.05%;其中两病感染最严重的是种猪场,其仔猪、后备母猪和经产母猪伪狂犬病毒感染率分别达到11.11%、70%和70%,圆环病毒2型感染率分别都达到100%,混合感染率高达51.72%。 相似文献
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猪圆环病毒病在规模化养猪场发生日益普遍,它已成为影响养猪业的主要传染病.我们从新乡市长垣县、封丘县、辉县市、卫辉市、获嘉县、原阳县、新乡县、延津县16个猪场采集各类猪的血清样品109份,其中断奶仔猪血清47份,后备母猪血清30份,经产母猪血清32份,应用酶联免疫吸附试验(ELISA)对以上血清样品进行猪圆环病毒Ⅱ型(PCV-2)血清抗体的检测.结果共检出阳性血清59份,总阳性率为54.13%(59/109),断奶仔猪抗体阳性率为48.94%(23/47),后备母猪抗体阳性率为56.67%(17/30),经产母猪抗体阳性率为59.38%(19/32).结果显示:所有被检猪场均有PCV-2抗体阳性猪存在,从检测结果可以看出,本市一些猪场正处于PCV-2感染的高发阶段.因此我们必须加强猪群净化、加强饲养管理、搞好环境卫生等综合防制对策来控制该病的发生. 相似文献
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为了确诊贵州省某规模化猪场保育仔猪异常死亡原因,从怀孕母猪、产房母猪、后备母猪、种公猪、哺乳仔猪、保育仔猪6个猪群采集90份血清样本采用ELISA方法分别进行血清抗体检测,并对采集的90份血清样本和1份病死猪淋巴结组织采用荧光PCR方法进行病原学检测。结果:6个猪群综合的猪瘟病毒、猪蓝耳病病毒、伪狂犬病病毒g B蛋白、伪狂犬病病毒g E蛋白血清抗体阳性率分别为87.78%、70.00%、88.89%、4.44%;猪瘟病毒、猪蓝耳病病毒、伪狂犬病病毒病原核酸检测显示阴性,猪圆环病毒2型病原核酸检测淋巴结组织样本显示阳性。试验结果表明,引起该猪场保育仔猪死亡的原因为猪圆环病毒2型感染。同时,怀孕母猪群出现了伪狂犬病病毒g E蛋白抗体阳性,提示怀孕母猪群可能存在猪伪狂犬病野毒感染。 相似文献
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从东莞市15个猪场采集猪血清169份,用ELISA方法进行了猪圆环病毒Ⅱ型(PCV-2型)抗体检测,共检出抗体阳性血清91份,总阳性率为53.8%;公母猪的阳性率为64.2%;仔猪及保育猪的阳性率为35.0%。血清学调查结果显示,东莞地区猪场PCV-2型的感染较普遍,同时,本文就断奶猪多系统衰竭综合征的综合防制进行了分析讨论。 相似文献
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2011年上半年,从湖南的茶陵、郴州、桃源、南县、望城和攸县,在没有进行圆环病毒2型疫苗接种的6个规模猪场共采集血样315份,其中经产母猪血样245份、后备母猪42份、哺乳仔猪17份、保育仔猪11份。用酶联免疫吸附试验(ELISA)进行圆环病毒2型的抗体检测。结果显示,猪群整体阳性率为95.2%,其中经产母猪群,后备母猪群,哺乳仔猪群和保育仔猪群的抗体阳性率分别为96.3%、97.6%、100%和54.5%。结果表明,PCV-2在湖南省规模猪场的各阶段猪群中感染非常普遍,应引起重视和加强防控,特别是保育仔猪群的抗体阳性率仅为54.5%,很大部分的抗体阴性保育仔猪存在感染发病的风险,这一结果与临床上保育仔猪群肾性皮炎高发情况相一致。 相似文献
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Sixteen cesarean-derived, colostrum-deprived piglets were inoculated intranasally with porcine circovirus type 2 (PCV2), originally isolated from a pig affected with postweaning multisystemic wasting syndrome (PMWS). At 1 day postinoculation (PI), 3 of the 5 piglets in the uninoculated control group were moved to the room of inoculated piglets for contact exposure. Porcine circovirus type 2 was detected by polymerase chain reaction (PCR) in swabs from inoculated piglets from 1 day PI and from contact piglets from 2 days after cohabitation. Porcine circovirus type 2 was also detected in all serum samples but not in control piglets 7 days PI. Until the end of study, PCV2 was detected in swabs and serum samples by PCR but not in the control piglets. One inoculated piglet died suddenly without clinical signs 19 days PI. Beginning at 14 days PI, 5 piglets, including 1 contact piglet, had clinical signs of depression, anorexia, and icterus, and 1 inoculated piglet died 21 days PI. Most of the piglets exhibiting the above clinical signs became moribund and were necropsied 21 and 28 days PI. In the piglets that showed clinical signs, gross lesions, including icterus of liver and hemorrhage in stomach, and typical histopathological lesions of PMWS, such as lymphoid depletion and basophilic intracytoplasmic inclusion bodies in lymph nodes and other tissues, were observed. Porcine circovirus type 2 was detected by PCR in all tissue samples except in those of the control piglets. Porcine circovirus type 2 was recovered from several tissue samples of the piglets necropsied until 35 days PI. In particular, PCV2 was recovered in high titer from most of the tissue samples of the piglets exhibiting clinical signs. Serum antibody against PCV2 was mostly detected in inoculated piglets and in contact piglets 14 and 21 days PI by an indirect fluorescence antibody test but was not detected in the piglets exhibiting clinical signs until 28 days PI. These results indicate that PCV2 was able to induce clinical PMWS in the absence of other swine pathogens and that there were significant differences in both the quantitative PCV2 distribution in tissues and the antibody response between the piglets that were infected and developed PMWS and those that were infected but remained healthy. 相似文献
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Staebler S Buergi E Litzenberger B McCullough K McNair I McNeilly F Pospischil A Sydler T 《Schweizer Archiv für Tierheilkunde》2004,146(10):461-8; discussion 469
Postweaning wasting is a major worldwide problem in pig production, particularly with respect to the disease termed postweaning multisystemic wasting syndrome (PMWS). In addition to wasting, PMWS symptoms include respiratory distress, diarrhoea, pallor and occasional cases of jaundice. The causative agent is porcine circovirus type 2 (PCV-2). The objective of the present study was to determine the significance of PMWS and similar conditions in Switzerland. A total of 72 weaned piglets from 26 farms showing wasting were examined for the presence of PCV-2 by immunohistochemical and histological analysis and 57 piglets from 21 farms were examined serologically. Possible causes for wasting other than PCV-2 were investigated by macroscopic, histological and bacteriological methods. PCV-2 antigen was identified immunohistochemically in the lymphatic organs in 11 of these 72 piglets. However, only 4 animals showed histological changes typical of PMWS. PCV-2 antibodies were found in 70% of the piglets. Piglets with wasting syndrome not associated with PCV-2 infection suffered from conditions including porcine proliferative enteropathy, gastric ulcers, polyserositis and polyarthritis. The most frequent condition was chronic enteritis not associated to circovirus infection. The results from the serological analyses indicate a wide distribution of PCV-2 in the Swiss pig population. However, confirmed cases of PMWS were rare in the investigated piglets. 相似文献
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猪圆环病毒2型(PCV2)感染后会导致断奶仔猪多系统衰竭综合征(PMWS)等"猪圆环病毒相关疾病"(PCVADs),给全球养猪业造成了巨大的经济损失。疫苗免疫是防控该病的有效手段,近年来市场上推出了PCV2灭活疫苗、嵌合疫苗和亚单位疫苗。PCV2变异较快,主要临床流行毒株从PCV2b逐渐演变为PCV2d,现有商业化疫苗免疫效果需进一步研究证实。论文就近年来国内外PCV2传统疫苗、新型基因工程疫苗、PCV2疫苗与其他疫苗联合免疫的研究进展进行综述,旨在为猪圆环病毒相关疾病的防控及疫苗的开发与利用提供参考。 相似文献
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Allan GM Caprioli A McNair I Lagan-Tregaskis P Ellis J Krakowka S McKillen J Ostanello F McNeilly F 《Zoonoses and public health》2007,54(5):214-222
Porcine circovirus type 2 (PCV2) infection is now recognized as the major factor in the development of post-weaning multisystemic wasting syndrome (PMWS). Although Koch's postulates have been fulfilled for PCV2 and PMWS, the severe clinical expression of the disease observed in field cases has been difficult to reproduce experimentally. Some studies have demonstrated that immune stimulation associated with the use of some commercially available swine vaccines may trigger progression of PCV2 infection to disease and lesions characteristic of PMWS. Here we describe the effects on PCV2 infection in an experimental model following the use of a commercially available modified live vaccine to porcine respiratory and reproductive syndrome virus (PRRSV). Although none of the piglets infected with PCV2 developed clinical PMWS, the severity of microscopical lesions and the PCV2 antigen load associated with these lesions were higher in the PRRSV-vaccinated piglets compared with those detected in the PCV2 only infected animals. 相似文献
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从四川省21个规模化猪场采集样品273份,利用PCR方法检测并分析了猪细小病毒和猪圆环病毒的感染及混合感染情况,结果共检出PPV病原阳性样品47份（占17．22％）;PPV阳性猪场8个（占38．1％）;种猪的感染率较高,仔猪感染率相对较低;检出PCV2病原阳性样品143份（占52．38％）,PCV2阳性猪场18个（占85．7％）;PCV2感染随猪年龄的增长而升高;检出PPV和PCV2混合感染样品29份（占10．62％）;同时存在PPV和PCV2的猪场6个（占28．7％）,混合感染主要集中在母猪和保育仔猪阶段。仅有3个猪场未检出PPV和PCV2病原,占14．3％。该结果说明PPV与PCV2及其混合感染在四川省流行广泛,对养殖业构成了较严重的危害。 相似文献
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为了解盐源县养猪场猪圆环病毒病感染情况,本实验采集养猪场猪血清样本1980头份和疑似病例组织样本135头份,分别采用ELISA和PCR方法进
行猪圆环病毒2型抗体和核酸检测,结果显示：在种猪场、商品猪场和农户猪场中,猪圆环病毒2型抗体的阳性率分别为37.50%(15/40)、
32.84%(582/1772)和29.76%(50/168),平均为32.68% (647/1980);在种公猪、经产母猪、育成猪和哺乳仔猪中,猪圆环病毒2型抗体的阳性率分别为
37.50%(3/8)、57.03%(73/128)、29.48%(342/1160)和33.47%(229/684);在种猪场、商品猪场和农户猪场疑似病例中,猪圆环病毒核酸检出率分别为
86.36%(19/22)、77.45%(79/102)和72.73%(8/11)。结果表明,盐源县养猪场已普遍存在猪圆环病毒感染,应加强该病的预防与控制。 相似文献
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对经过流行病学调查、临床诊断、病理剖检、病原学检查确诊为猪圆环病毒感染的病猪放血处死,采集肺脏、脾脏、全身淋巴结等组织制作同源组织灭活苗.选取体重相近、有临床发病表现的仔猪160头,随机分成A、B、C、D 4组,其中A组为同源组织灭活苗+兽大菌灭注射液,B组为兽大菌灭注射液,C组为杂症一针注射液,D组为对照组,每组40头.每天用药1次,连用3 d,在相同条件下进行饲养管理,结果A、B、C、D 4组的成活率分别为95.0%、80.0%、45.0%、17.5%. 相似文献
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Postweaning multisystemic wasting syndrome (PMWS) is a worldwide emerging disease of weaned piglets. The objective of this review is to summarize the current knowledge regarding PMWS, its causative agent, mode of transmission, diagnostic techniques to detect PCV-2, the possible control measures, and the association of PMWS and PCV-2 with porcine dermatitis and nephropathy syndrome (PDNS). The causative agent of PMWS is porcine circovirus type 2 (PCV-2), however, not all pigs infected with PCV-2 develop the syndrome. PCV-2 is consistently associated with PMWS and PMWS is considered not to occur without it. Both the syndrome and the virus are not regarded as new. Co-factors that could activate PCV-2 to cause PMWS are considered. This enigmatic nature of both the syndrome and the virus is triggering a concern towards uncertainties of the viral transmission, its introduction in to the herd, effective tools of diagnosis, and control strategies. 相似文献
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Krakowka S Ellis J McNeilly F Waldner C Rings DM Allan G 《The Canadian veterinary journal. La revue veterinaire canadienne》2007,48(7):716-724
Groups (5 to 15 per group) of gnotobiotic swine were infected oronasally with porcine circovirus type 2 (PCV2) at 3 days of age and then given 1 of 6 different commercial Mycoplasma hyopneumoniae (M. hyopneumoniae) bacterins as either a single dose (7 d of age, 1 application products) or 2 doses (7 and 21 d of age, 2 application product). Control groups received PCV2 alone (n = 9) or were infected with PCV2 and immunized twice with keyhole limpet hemocyanin (KLH) emulsified in incomplete Freund's adjuvant (ICFA) (n = 7). Five of 7 (71%) PCV2-infected piglets immunized with KLH/ICFA developed mild or overt PMWS, whereas none of 9 piglets infected with PCV2 alone developed PMWS. Five of 12 (42%) piglets vaccinated with a commercial bacterin containing mineral oil adjuvant developed PMWS following vaccination. None of the PCV2-infected piglets in the other bacterin-vaccinated groups developed PMWS in this model of PCV2-associated disease. This difference in prevalence of PMWS in piglets given the mineral oil-adjuvanted M. hyopneumoniae bacterin and the other M. hyopneumoniae bacterin vaccination groups was statistically significant (P < 0.05). 相似文献
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Postweaning multisystemic wasting syndrome (PMWS) was reproduced in prenatally porcine circovirus 2 (PCV2)-infected pigs by either postnatal infection with porcine parvovirus (PPV) or by immunostimulation. Twenty-four randomly selected piglets from 3 sows, which had been experimentally infected during gestation with PCV2, were randomly divided into 3 groups; group 1 (prenatal PCV2 infection, with postnatal PPV infection), group 2 (prenatal PCV2 infection, with postnatal keyhole limpet hemocyanin, emulsified in incomplete Freund's adjuvant [KLH/ICFA] injection), and group 3 (prenatal PCV2 infection only). Twenty-four randomly selected piglets from 3 uninfected sows were randomly divided into 3 groups; group 4 (no prenatal infection, with postnatal PCV2 and PPV infection), group 5 (no prenatal infection, with postnatal PCV2 infection), and group 6 (negative control pigs). Body weight in negative control pigs (group 6) was increased significantly compared with pigs in groups 1, 2, and 4 at 49, 52, 56, 59, and 63 days of age. The granulomatous inflammatory reaction and lymphoid depletion that are typical lesions in pigs with PMWS were observed in the lymph node of piglets in groups 1, 2, and 4 at 63 days of age. Pigs in group 3 had significantly fewer PCV2-positive cells than those from groups 1, 2, 4, or 5. When the prenatally PCV2-infected pigs were infected with PPV or injected with immunostimulant in the postnatal period, they developed PMWS. Thus, factors that potentiate the progression of prenatal PCV2 infection to PMWS are postnatal infection with PPV or immune stimulation. 相似文献