首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 484 毫秒
1.
选择Ｓ１基因做为目的基因,运用ＲＴ－ＰＣＲ技术分别扩增１２株以非免疫鸡胚繁殖并通过超速离心浓缩的鸡胚尿囊液中的ＩＢＶ,所用引物为ＩＢＶＢｅａｕｄｅｔｔｅ株Ｓ１基因两侧的对应序列,跨幅为１．７２ｋｂ。结果６株ＩＢＶ毒株（ＳＡＩＢ３、Ｆ、Ｄ４１、Ｍ４１、Ｈ５２、Ｈｏｌｔｅ）扩增出了长约１．７ｋｂ的Ｓ１基因片段,而另６株ＩＢＶ毒株虽经４次ＲＴ－ＰＣＲ重复后也显阴性。用ＨａｅⅢ内切酶对６个毒株的ＲＴ－ＰＣＲ产物进行酶切,结果显示Ｍ４１、Ｈ５２、Ｄ４１３个ＩＢＶ毒株的Ｓ１基因包含两个ＨａｅⅢ位点,Ｆ株与Ｈｏｌｔｅ株Ｓ１基因包含１个ＨａｅⅢ位点,而ＳＡＩＢ３株Ｓ１基因则已丢失ＨａｅⅢ位点。实验结果表明：运用ＲＴ－ＰＣＲ技术检测ＩＢＶ时,由于Ｓ１基因的核酸序列具有较高的变异率,因此Ｓ１基因不宜做为目的基因。  相似文献   

2.
用京－１株鸡马立克氏病（ＭＤ）强毒感染１￣１０个家系１日龄的ＢＷＥＬ－ＳＰＦ鸡,观察比较临床症状和剖检病变及病理切片,以此评估该ＢＷＥＬ－ＳＰＦ鸡群每个家系鸡对京－１株ＭＤ强毒的敏感性。结果,１,５,８,９号家系的ＢＷＥＬ－ＳＰＦ鸡对京－１株ＭＤ强毒最敏感;３,４,５,６,１０号家庭系次之,２、７号家系敏感性相对较差。  相似文献   

3.
从母牛接种过Ａ组ＢＲＶ株ＮＣＤＶ－Ｌｉｎｃｏｌｎ（Ｐ１：Ｇ６）苗的两个吡邻牛场分离到两株牛轮状病毒（ＢＲＶ），编号为ＮＳ－１和ＮＳ－２，Ｎｏｒｔｈｅｒｎ－ｂｌｏｔｔｉｎｇ试验表明，ＮＳ－１和ＮＳ－２有相的的Ａ组ＲＮＡ电泳模式，而且全部基因片段同源，体外中和试验表明，ＮＳ－１株病毒能被Ｇ６特异性单克隆抗体（ＭｃＡｓ）和抗ＢＲＶＢ６４１株（Ｐ５：Ｇ６）豚鼠高免血清（ＧＰＡＳ）中和，但不被Ｇ１０特异性Ｍ  相似文献   

4.
牛传染性鼻气管炎病毒tk基因的克隆   总被引：2，自引：0，他引：2  
王柳  于力 《中国兽医学报》1995,15(2):116-120
根据牛传染性鼻气管炎病毒ＬＡ株的物理图谱及Ｃｏｐｅｒ株和ＬＡ标ｔｋ基因在基因组中的定位，将ＬＡ株ＤＮＡ ＨｉｎｄⅢＡ片段中的ＳａｌＩ－ＳａｌＩ亚片段、ＢｇｌⅢ－ＳａｌＩ双酶切亚片段分别克隆到载体质粒ｐＢｌｕｅｓｃｒｉｐｔＳＫ中，筛选出３个重组质粒ｐ＾ｔｋ－１，Ｐｔｋ－２和Ｐｔｋ－３，其外源片段大小分别为２．７ｋｂ，１．１ｋｂ和１．６ｋｂ。经酶切分析及同源核酸探针杂交试验表明，２．７ｋｂ ＳａｌＩ－  相似文献   

5.
应用RT—PCR技术扩增禽传染性支气管炎病毒S1基因的研究   总被引：2，自引：0，他引：2  
选择Ｓ１基因做为目的基因,运用ＲＴ－ＰＣＲ技术分别扩增１２株以非免疫鸡胚繁殖并通过超速离心浓缩的鸡胚尿囊液中的ＩＢＶ,所用引物为ＩＢＶ Ｂｅａｕｄｅｔｔｅ株Ｓ１基因两侧的对应序列,跨幅为１．７２ｋｂ。结果６株ＩＢＶ毒株（ＳＡＩＢ３、Ｆ、Ｄ４１、Ｈ５２、Ｈｏｌｔｅ）扩增出了长约１．７ｋｂ的Ｓ１基因片段,而另６株ＩＢＶ毒株虽经４次ＲＴ－ＰＣＲ重复后也显阴性。用ＨａｅⅢ内切酶对６个毒株的ＲＴ－ＰＣＲ产物  相似文献   

6.
鸡传染性支气管腺胃病变型毒株免疫原基因的分离鉴定   总被引：4，自引：0，他引：4  
以腺胃病型变型鸡传染性支气管炎病毒分离株Ｈ９５提取的ＲＮＡ为模板,按ＩＢＶ Ｂｅａｕｄｅｔｔｅ株Ｓ１基因两侧对应序列,设计一对２７ｂｐ引物,引物间跨幅为１．７Ｋｂ。用反转录－聚梧酶链反应获得与预期大小一致的核酸片段。ＲＴ－ＰＣＲ产物凝胶电泳后,Ｓｏｕｔｈｅｒｎ印迹,经Ｓ１探针检测呈阳性,表明获得分离株Ｈ９５Ｓ１基因。  相似文献   

7.
参照澳大利亚鸡传染性法氏囊病病毒（ Ｉ Ｂ Ｄ Ｖ）００２７３ 毒株基因组序列设计的 １ 对引物,位于 Ｖ Ｐ２ 高可变区两端,通过 Ｒ Ｔ Ｐ Ｃ Ｒ,扩增了 ５ 个 Ｉ Ｂ Ｄ Ｖ 山东分离株 Ｖ Ｐ２ 基因的 ６０７～１ ０８０ 位核苷酸片段（ａａ２０３～３０６）。 Ｐ Ｃ Ｒ 产物经纯化后,分别用 Ｄｒａ Ⅰ、 Ｓａｃ Ⅰ、 Ｓｓｐ Ⅰ、 Ｐｓｔ Ⅰ和 Ｓａｕ３ Ａ Ｉ共 ５ 种限制性内切酶（ Ｒ Ｅ）进行消化处理,结果 ５ 个分离株均为 Ｄｒａ Ⅰ（－ ）、 Ｓａｃ Ⅰ（－ ）和 Ｓａｕ ３ Ａ Ｉ（＋ ）, Ｌ Ｃ２ 和 Ｔ Ａ 株为 Ｓｓｐ Ⅰ（＋ ）, Ｌ Ｃ１ 和 Ｊ Ｎ 株为 Ｓｓｐ Ⅰ（－ ）, Ｌ Ｃ１ 和 Ｌ Ｃ２ 株为 Ｐｓｔ Ⅰ（＋ ）, Ｊ Ｎ、 Ｌ Ｌ 和 Ｔ Ａ 株为 Ｐｓｔ Ⅰ（－ ）;５ 个分离株的酶切图谱与美国变异株迥异,而 Ｔ Ａ 株与日本超强毒株 ９０１１ 相类似。５ 个分离株与现用疫苗株对比,至少在 Ｖ Ｐ２ 可变区内存在着一定的差异,这可能是 Ｉ Ｂ Ｄ Ｖ 接种免疫鸡群仍然暴发 Ｉ Ｂ Ｄ 的原因之一。  相似文献   

8.
传染性支气管炎病毒（ＩＢＶ）分离株ＱＤ经ＲＴ－ＰＣＲ扩增出约１．７ｋｂＳ１糖蛋白基因ｃＤＮＡ，修饰后插入ｐＵＣ１８ＳｍａｌⅠ／ＥｃｏＲＩ位点构成质粒ｐＵＣＱＤＳ１。对克隆的Ｓ１基因５′端高变区序列测定显示，起始密码上游６０碱基和下游３８０碱基中与参考株Ｍ４１Ｓ１基因序列仅有１个碱基的差异，表明ＱＤ为一类Ｍ４１分离株。  相似文献   

9.
利用反转录套式聚合酶链反应技术从北京地区３株鸡性支气管炎病毒（ＩＢＶ）分离株中扩增出１０５４ｂｐＳ１基因高变区。利用限制性内切酶ＳｉｎＩ和ＰｓｔⅠ的酶切位点分析图谱进一步证实了ＲＴ－ｎｅｓｔｅｄＰＣＲ的特异性。将３段Ｓ１基因分别克隆到ＰＵＣ１９质粒中,获得重组质粒ＰＵＣＩＢＶＳ１－ＢＪ１,ＰＵＣＩＢＶＳ１－ＢＪ２和ＰＵＣＩＢＶＳ１－ＢＪ３。  相似文献   

10.
传染性支的管炎病毒（ＩＢＶ）分离株ＱＤ经ＲＴ－ＰＣＲ扩增出约１．７ｋｂＳ１糖蛋白基因ｃＤＮＡ，修饰后插入ｐＵＣ１８ＳｍａｌⅠ／ＥｃｏＲＩ位点构成质粒ｐＵＣＱＤＳ１。对克隆的Ｓ１基因５′端高变区序列测定显示，起始密码上游６０碱基和下游３８０碱基中与参考株Ｍ４１Ｓ１基因序列仅有１个碱基的差异，表明ＱＤ为一类Ｍ４１分离株。  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
Tropical Animal Health and Production -  相似文献   

14.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

15.
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.  相似文献   

16.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

17.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

18.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

19.
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors.  相似文献   

20.
<正>1.INTRODUCTION Ensuring transportation network security is one Of the most daunting challenges confronting homeland security agencies today.Significant research has been dedicated.To model and analyze the vulnerability of transportation systems,while notably fewer studies propose specific strategies for deploying defensive technologies to safeguard these systems.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号