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１９９５年６～１０月和１９９６年６～８月对中国对虾养及其与台湾红非鲫、菲律宾蛤仔、海湾扇贝或缢蛏的不同混养实验围隔浮游生物的研究结果表明,养管理措施和放养种类对围隔浮游生物种类和生物量都会产生一定影响,其中前者往往具主要作用。投饵养殖时围隔浮游植物以２０μｍ以下的种类为主,生物量较低,其中混养台湾红非鲫的围隔叶绿素ａ（Ｃｈｌ－ａ）相对较高,混养菲律宾蛤仔的围隔Ｃｈｌ－ａ较低;在投饵的基础上施肥后甲 相似文献
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不同单养和混养海水实验围隔初级生产力的研究 总被引：3，自引：1，他引：2
１９９５年和１９９６年夏秋季进行的围隔实验表明投饵养殖时浮游植物毛初级生产力较低,水呼吸相对较高,多数围隔中毛初级生产力／水呼吸〈１,其中混养中国对虾和台湾红罗非鱼的围隔中毛初级生产力较高,而混养中国对虾,台湾红罗非鱼和菲律宾蛤仔的围隔中毛初级生产力最低;在投饵基础上酌情施肥后围隔毛初级生产力同于投饵养殖时,毛初级生产力／水呼吸〉１,但单养中国对虾,对虾与罗非鱼混养和对虾与海湾扇贝或缢蛏混养的围隔 相似文献
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于１９９５年６－９月间在施肥鱼贝混养生态系中，实验研究了搭配养殖菲律宾蛤仔对浮游生物的效应和浮游生物对菲律宾蛤仔养殖的影响。在浮游生物群落构成方面，菲律宾蛤仔使蓝藻、甲藻和浮游桡足类显著减少。对生物量和初级生产力的影响程度与养殖密度有关，而浮游生物丰度和初级生产力在时间上的变化又与营养盐有关。 相似文献
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不同单养及混养海水实验围隔水化学的研究 总被引：5，自引：1，他引：4
１９９５年６～１０月和１９９６年６～８月对不同单养和混养海水实验围隔的水化学研究结果表明：围隔中盐度、总氮、总磷和化学耗氧量（ＣＯＤ）在雨季前逐渐升高,进入雨季后降低;投饵养殖时围隔中磷浓度较低氮浓度较高,其中混养中国对虾和台湾红罗非鱼的围隔活性磷（ＰＯ４－Ｐ）浓度相对较高,混养对虾与菲律宾蛤仔的围隔氨氮（ＮＨ３－Ｎ）浓度较高,投铒兼酌情施肥养殖时围隔中氮浓度较低磷浓度较高,其中混养中国对虾与缢蛏 相似文献
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自从1993年我国海水养殖虾类遭受白斑病毒病危害以来,海水虾类养殖一直处于不稳定的状态。一般虾农抗风险能力差,不贸然在天然海水池塘中单养虾类,而是多品种混养,大大降低了由于病害而绝产的风险。下面介绍虾、蟹、贝混养,四放五收。这种方法混养的品种为三疣梭子蟹、日本对虾和菲律宾蛤仔。 相似文献
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１９８９，１９９０年利用荒废虾塘进行以中国对虾为主混养尼罗罗非鱼，鲻，缢蛏，毛蚶，菲律宾蛤仔等多品种立体养殖试验，结果：１９８９，１９９０年，鱼虾贝平均每亩总产分别为５００ｋｇ和５６０．９ｋｇ。 相似文献
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2007～2008年在莆田后海围垦区进行菲律宾蛤仔反季节高滩位高效生态养殖,收获的菲律宾蛤仔平均规格115粒/kg,平均产量6247.5kg/hm2,较传统养殖模式提高效益28%。藻类池与养殖池浮游植物有34种,优势种主要有中肋骨条藻Skeletonema costatum、反曲原甲藻Prorocen-trumsigmoides、利马原甲藻Prorocentrumlima、新月拟菱形藻Nitzschiella closterium、海洋原甲藻Prorocentrum micans。中肋骨条藻在不同的月份中均为最主要的优势种。不同月份各池浮游植物细胞丰度的变化范围为(0.02～190)×104cells/L。2007年11月与2008年3月浮游植物细胞丰度较高,2008年1、2月藻类细胞丰度相对较低。通过对池中水温、透明度、无机氮、无机磷、DO、浮游植物多样性指数和均匀度的分析,说明菲律宾蛤仔池大量施肥已很大程度影响了浮游植物的种类组成与细胞丰度。养殖过程中浮游植物生物多样性较好、均匀度较低、优势度高,菲律宾蛤仔适口饵料中肋骨条藻等优势种类大量繁殖,有利于提高菲律宾蛤仔的产量。 相似文献
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鲢、鳙对三角帆蚌池塘藻类影响的围隔实验 总被引：6，自引：3，他引：3
以浙江金华汤溪威旺养殖基地的三角帆蚌养殖水体为研究对象,通过围隔实验比较研究了单养鲢、鳙和三角帆蚌的池塘浮游植物密度、生物量和优势种(属)组成等的差异,以及养蚌池混养鲢鳙对水体浮游植物密度、生物量以及优势种变化的影响。结果表明,养蚌(10#)围隔的浮游植物平均密度和生物量均显著高于高密度鲢(12#)围隔(P<0.05),其蓝藻数量及生物量显著高于高密度鲢(12#)和低密度鳙(13#)围隔(P<0.05),绿藻数量则显著低于低密度鲢单养(11#)围隔(P<0.05)。在鱼蚌混养的情况下,单养蚌(10#)围隔浮游植物平均数量显著高于鲢-蚌混养(15#,16#)和鳙-蚌混养(17#,18#)围隔(P<0.05),其蓝藻数量及生物量极显著高于鲢-蚌混养(15#,16#)或鳙-蚌(17#,18#)围隔(P<0.01),其绿藻数量显著低于混养高密度鲢(16#)或低密度鳙(17#)的混养围隔(P<0.05)。研究结果充分说明,鲢、鳙和三角帆蚌三者对水体藻类组成的影响有别,三角帆蚌养殖池中适当混养鲢或鳙可以有效控制蓝藻(铜绿微囊藻)的生长,促进绿藻(四尾栅藻)的生长,并最终有利于三角帆蚌的养殖,混养鲢密度的增加有利于控制藻类生长,而鳙密度的增加促进了裸藻等中大型藻类的生长。 相似文献
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Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean 
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下载免费PDF全文 The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna. 相似文献
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In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg. 相似文献
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Liping Li Rui Wang Wanwen Liang Xi Gan Ting Huang Yan Huang Jian Li Yunliang Shi Honglin Luo Ming Chen 《广西水产科技》2014,(1)
Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China. 相似文献
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Iciar Martinez Amaya Velasco Ricardo Pérez-Martín Eskil Forås Michiaki Yamashita Carmen G. Sotelo 《Journal Of Aquatic Food Product Technology》2013,22(4):289-297
The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus. 相似文献
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Growth hormone (GH) and reproduction: a review 总被引：5，自引：0，他引：5
Florence Le Gac Odile Blaise Alex Fostier Pierre-Yves Le Bail Maurice Loir Brigitte Mourot Claudine Weil 《Fish physiology and biochemistry》1993,11(1-6):219-232
Interaction between growth and reproduction occurs in many vertebrates and is particularly obvious at certain stages of the life cycle in fish. Endocrine interactions between the gonadotropic axis and the somatotropic axis are described, the potential role of GH being emphasised. A comparative analysis of these phenomena in mammals, amphibians and fish, suggests a specific role of GH in the physiology of puberty, gametogenesis and fertility. It also shows the original contribution made by studies on the fish model in this field of investigations. 相似文献
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Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL?1 (1.25 ng well?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation. 相似文献
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Controlled infection of Poecilia reticulata Peters (guppy) with Tetrahymena by immersion and intraperitoneal injection 下载免费PDF全文
下载免费PDF全文 G Sharon M Pimenta‐Leibowitz M C L Vilchis N Isakov D Zilberg 《Journal of fish diseases》2015,38(1):67-74
Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality). 相似文献
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