共查询到19条相似文献，搜索用时 265 毫秒
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为评价含碘、季铵盐、过硫酸氢钾、二氧化氯这四类常用消毒剂对非洲猪瘟病毒（ASFV）的灭活效果,参考OIE参考实验室相关操作流程,基于畜禽栏舍、运载工具、器具消毒及皮肤黏膜消毒目的,根据说明书标明的浓度范围选择不同工作浓度,与ASFV分别在4 ℃和20 ℃条件下作用30分钟,10倍连续稀释后接种猪肺泡巨噬细胞,同时加入猪红细胞,培养观察红细胞吸附现象。结果显示,本试验四类消毒剂对ASFV均有灭活作用,但灭活效果因消毒剂的种类和工作浓度不同有所差异。相同工作浓度的同一消毒剂产品,在4 ℃和20 ℃这两种条件下的灭活效果基本一致。季铵盐类和二氧化氯类消毒剂,在说明书推荐的工作浓度下,均有效灭活ASFV;多数含碘类、过硫酸氢钾类消毒剂,需在其说明书标明浓度范围的较高工作浓度时,才可有效杀灭ASFV。本研究为非洲猪瘟防控工作中消毒剂的选择和使用提供参考。 相似文献
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孙忠慧 《江西畜牧兽医杂志》2019,(2):47-48
选择有效的消毒药物和消毒方式,定期开展消毒灭源,是防控非洲猪瘟流行的有效手段。主要消毒剂有次氯酸盐、戊二醛、复合酚、过氧乙酸、火碱、百毒杀、碘类等。1 猪群饮用水消毒猪群饮水消毒可使用2%~3%的次氯酸钠消毒。次氯酸钠含有效氯10%~12%,通常为无色或稍带浅黄绿色的液体,存在铁时呈红色。是一种常用的高效、广谱消毒剂,可有效杀灭细菌、芽孢、病毒、真菌等多种微生物。 相似文献
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为探究常见消毒剂对非洲猪瘟病毒(ASFV)的灭活效果,本试验于体外检测了10种市售消毒剂对生长在猪肺泡巨噬细胞中ASFV的灭活效果。进而用3种消毒剂对曾经暴发非洲猪瘟(ASF)的废弃猪场进行环境消毒,并用荧光定量PCR方法验证其可靠性。结果显示,在各消毒剂对细胞的安全浓度下,次氯酸不具有灭活ASFV的作用;过硫酸氢钾复合物作1∶200稀释后,作用0.5 h能完全灭活ASFV;二氯异氰脲酸钠粉和苯扎溴铵稀释800倍后,能在0.5 h内有效灭活ASFV;其他消毒剂在其安全稀释度的浓度下,0.5 h内能完全灭活ASFV。本试验可为养殖场防控ASFV的感染提供消毒剂筛选的参考依据。 相似文献
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选择有效的消毒药物和消毒方式,定期开展消毒灭源,是防控非洲猪瘟流行的有效手段。主要消毒剂有次氯酸盐、戊二醛、复合酚、过氧乙酸、火碱、百毒杀、碘类等。猪群饮用水消毒猪群饮水消毒可使用2%~3%的次氯酸钠消毒。次氯酸钠为无色至浅黄绿色液体,存在铁时呈红色,含有效氯10%~12%。为高效、快速、广谱消毒剂,可有效杀灭各种微生物,包括细菌、芽孢、病毒、真菌等。饮水的消毒,每立方米水加药30~50 mg,作用30 min. 相似文献
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《养殖与饲料．饲料世界》2020,(9)
非洲猪瘟(ASF)是由非洲猪瘟病毒(ASFV)导致的一种烈性、高度接触性传染病,目前兽医临床上还没有有效的商品化非洲猪瘟疫苗,该病的防控应重视生物安全措施,提高饲养管理水平和消毒措施。非洲猪瘟病毒对碱类、戊二醛等多种消毒剂均敏感,根据消毒范围可选择合适的消毒剂。 相似文献
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《中国兽医杂志》2020,(1)
非洲猪瘟(简称非瘟)是猪病中一种重要的疾病,严重影响养猪业的发展。因目前没有有效疫苗和治疗药物,对该病的防控主要采取消除传染源和生物安全措施。非瘟发病场消毒是清除病原的重要手段。本文对江苏某发生非瘟猪场的猪舍内进行了消毒,并评价了每个消毒环节的效果。采取的消毒措施为:清扫前消毒,清扫冲洗,之后分别用3%NaOH、5%次氯酸钠、1∶200过硫酸氢钾消毒和冲洗。干燥后火焰灼烧、白化处理(4%火碱+20%石灰水)。最后用甲醛熏蒸24 h,空舍10 d。消毒效果检测表明,经过多个环节的消毒到过硫酸氢钾作用完成时,环境细菌总数已达到200 CFU/cm~2,非洲猪瘟病毒(ASFV)核酸荧光定量PCR Ct值为36,表明环境细菌总数和ASFV核酸已非常少。火焰灼烧后,排污沟细菌总数为43 CFU/cm~2,达到环境合格标准;ASFV为阴性。再经过白化、熏蒸、空舍后细菌总数达标,ASFV为阴性。本试验表明,非瘟后猪舍经过多个消毒环节,清除了环境中ASFV。本研究为非瘟后猪舍的合理消毒程序和消毒后效果检测提供参考。 相似文献
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本实验旨在评价保存液对唾液中非洲猪瘟病毒核酸的保护效果。通过以下分组进行实验:A组,5 ml唾液+50μl阳性样本(100倍稀释);B组,5 ml唾液+5μl阳性样本(1000倍稀释);C组,棉签沾取100倍稀释样品放入保存液;D组,棉签沾取100倍稀释样品放入生理盐水;E组,原唾液(空白)样本。在1 h、7 h、22 h和35 h这个四个时间点,分别通过柱式和磁珠法提取非洲猪瘟病毒的核酸,并通过荧光定量PCR检测样品中的病毒核酸含量,结果表明,无论是柱式法还是磁珠法提取,A、B和D组的Ct值随着时间推移均在变大,而C组(保存液)Ct值趋于稳定,说明保存液对非洲猪瘟病毒降解具有一定的减缓效果。 相似文献
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Solutions of ethylenediaminetetraacetic acid (EDTA)-Tris showed synergistic or additive effects on gram-negative bacteria when combined with hatchery disinfectants consisting of phenol and detergent (Magnaphen-100), quaternary ammonium compound (QAC) and glutaraldehyde (Synergize), QAC (BioSentry 904), and hydrogen peroxide. The gram-positive bacteria reacted less favorably, with reaction mixtures showing all three levels of potentiation (synergistie, additive, and antagonistic). Combinations of EDTA-Tris and a commercial glutaraldehyde solution (Glutracide), when mixed with the test organisms, showed mostly antagonistic effects. Solutions of EDTA-Tris decreased the concentration of hatchery disinfectants required for bacterial killing by 75% in those situations in which synergistic potentiation occurred. EDTA-Tris is nontoxic to 12-day-old embryos. Serial passage of the test organisms in solutions of EDTA-Tris did not result in the development of resistant forms. 相似文献
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为探究非洲猪瘟病毒(African swine fever virus,ASFV)标准物质作为试剂盒评价体系的可行性,比较了市场上5种主流品牌ASFV荧光PCR检测试剂盒的检测性能。使用ASFV P72基因核酸标准物质作为模板,根据5种试剂盒说明书分别进行相应的荧光定量PCR检测,结合扩增曲线、Ct值,分析不同试剂盒的敏感性、可重复性以及所需反应时间。结果显示:5种试剂盒的阴性、阳性对照均成立,最低检测限均为5.9×10-1拷贝/μL;4个厂家的试剂盒线性关系R2>0.98,其中最优的R2=0.994,离散度最小;各试剂盒的实际反应耗时与理论反应耗时均有一定差异(0.20~0.96 h)。结果表明,各生产厂家使用P72基因作为靶基因研制的ASFV荧光PCR检测试剂盒都可以使用ASFV标准物质作为评价体系,来评判试剂盒的检测性能。本试验为各实验室不同样品检测的ASFV荧光PCR检测试剂盒选择提供了一种可用的评价方法。 相似文献
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Shirai J Kanno T Tsuchiya Y Mitsubayashi S Seki R 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(1):85-92
The effects of three representative disinfectants, chlorine (sodium hypochlorite), iodine (potassium tetraglicine triiodide), and quaternary ammonium compound (didecyldimethylammonium chloride), on several exotic disease viruses were examined. The viruses used were four enveloped viruses (vesicular stomatitis virus, African swine fever virus, equine viral arteritis virus, and porcine reproductive and respiratory syndrome virus) and two non-enveloped viruses (swine vesicular disease virus (SVDV) and African horse sickness virus (AHSV)). Chlorine was effective against all viruses except SVDV at concentrations of 0.03% to 0.0075%, and a dose response was observed. Iodine was very effective against all viruses at concentrations of 0.015% to 0.0075%, but a dose response was not observed. Quaternary ammonium compound was very effective in low concentration of 0.003% against four enveloped viruses and AHSV, but it was only effective against SVDV with 0.05% NaOH. Electron microscopic observation revealed the probable mechanism of each disinfectant. Chlorine caused complete degeneration of the viral particles and also destroyed the nucleic acid of the viruses. Iodine destroyed mainly the inner components including nucleic acid of the viruses. Quaternary ammonium compound induced detachment of the envelope of the enveloped viruses and formation of micelle in non-enveloped viruses. According to these results, chlorine and iodine disinfectants were quite effective against most of the viruses used at adequately high concentration. The effective concentration of quaternary ammonium compound was the lowest among the disinfectants examined. 相似文献
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为了解贵州省某舍饲鸭场环境中菌落分布情况以及市售五种常用消毒剂(苯扎溴铵、月苄三甲氯铵、复方戊二醛、戊二醛癸甲溴铵、聚维酮碘)的灭菌效果。通过采集鸭场空气、粪便、垫料、饮水样本进行菌落总数测定;分别配制最小杀菌浓度的苯扎溴铵、月卞三甲氯铵、复方戊二醛及戊二醛癸甲溴铵4种消毒剂,采用雾线、喷洒消毒方式对舍饲鸭场空气、漏缝地板、料槽、蛋框及运输车车轮进行现场消毒;配制最小杀菌浓度的聚维酮碘,采用涂抹方式对鸭蹼部皮肤进行涂抹消毒;最后分别测定鸭场空气、漏缝地板、料槽、蛋框、运输车车轮及鸭蹼样本消毒前后的菌落总数并计算5种消毒剂对细菌的杀菌率。结果显示：舍饲鸭场空气菌落总数为3.79×104 CFU/m3,超出NY/T 388-1999畜禽场环境质量标准规定数值(25000 CFU/m3);粪便菌落总数为2.67×107 CFU/g,超出GB18596-2001畜禽养殖业污染物排放标准规定数值(1.03×106CFU/g);垫料菌落总数为3.7×106CFU/g,超出NY/T1167-2006畜禽场环境质量及卫生控制规范规定的清洁土壤数值(5×104CFU/g);饮水菌落总数为12 CFU/mL,符合GB 5749-2006生活饮用水卫生标准(100CFU/mL)。5种消毒剂对鸭场进行现场消毒试验,复方戊二醛对漏缝地板、蛋框、运输车车轮的细菌杀菌率最高,分别为96.78%、81.31%、87.5%;戊二醛癸甲溴铵对鸭舍空气、料槽的细菌杀菌率最高,分别为34.85%、74.35%;聚维酮碘对鸭蹼部皮肤细菌杀菌率为72.45%;4种消毒剂对漏缝地板、料槽、蛋框、运输车车轮消毒效果良好,对空气的消毒效果不理想;聚维酮碘对鸭蹼部皮肤消毒效果不理想。结果表明：该舍饲鸭场存在大量超过国标菌落总数的安全隐患,通过对该鸭场微生物菌落计数及现场消杀试验,在一定程度将菌落总数控制在了合理范围。研究结果旨在为掌握舍饲鸭场微生物菌落分布情况,制定舍饲鸭场消毒程序以及科学防控养鸭场细菌性疾病提供参考依据。 相似文献
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FENG Chun-yan DU Fang-yuan LIU Dan-dan Pershin Andrey WANG Cai-xia ZHANG Yong-ning WU Shao-qiang LIN Xiang-mei 《中国畜牧兽医》2017,44(11):3287-3293
African swine fever (ASF) is an infectious disease caused by the African swine fever virus (ASFV). In order to ensure the accuracy and reliability of the test results,it is necessary to develop positive standard control products used in the kit. The study was aimed to develop the virus-like particles containing African swine fever virus (ASFV) nucleic acid sequence and its application in detection method. Fristly,the full-length gene fragment of p72 gene was amplified, and the ASF DNA virus-like particles containing p72 gene was constructed using insect baculovirus system. In order to further validate the reliability of the virus-like particles in the application of the method,DNA nucleic acid was extracted simultaneously with the cultured virus and infected tissue sample and applied in the Real-time quantitative PCR method. The results showed that the virus-like particles prepared by this study could replace the ASFV as a positive control product in the Real-time quantitative PCR method, and could act as quality control during nucleic acid extraction process. In the fluorescent PCR detection kit, the lowest packaging concentration of virus-like particles was 102 TCID50. Further studies had shown that the virus-like particles were also suitable for common PCR and LAMP detection methods, the minimum concentration were 103 and 101 TCID50, respectively. The results of this study would be important for the ASF detection method, promoting the application of the method and ensuring the accuracy and reliability of the test results. 相似文献
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非洲猪瘟（African swine fever,ASF）是由非洲猪瘟病毒（African swine fever virus,ASFV）引起的烈性传染病,为了保证其检测结果的准确性和可靠性,需要研制试剂盒中使用的阳性标准质控品。本试验旨在研制含ASFV核酸序列的病毒样颗粒,并探究其在检测方法中的应用。首先扩增p72基因的全长片段,利用昆虫杆状病毒系统,包装出含有p72基因的ASF DNA病毒样颗粒。为了进一步验证该病毒样颗粒在应用中的可靠性,本研究将病毒样颗粒与ASF的组织毒及细胞毒同时进行DNA核酸提取,进行实时荧光定量PCR。结果表明,本研究制备的病毒样粒子能很好的取代ASFV在实时荧光定量PCR检测方法中作为阳性质控品,且能对核酸提取过程进行质控,实时荧光定量PCR检测试剂盒中,病毒样粒子的最低包装浓度为102 TCID50。进一步研究发现该病毒样颗粒也适用于普通PCR及LAMP检测方法中,最低浓度分别为103和101 TCID50。本试验结果将为规范ASF检测方法,促进ASF检测方法的转化应用及保证检测结果的准确度和可靠性提供科学依据。 相似文献