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1.
Xiaoping Wu Kun Qian Aijian Qin Haiyu Shen Pingping Wang Wenjie Jin Yassir Mohammed Eltahir 《Veterinary research communications》2010,34(7):619-632
Background
Five isolates (JS09GY2, JS09GY3, JS09GY4, JS09GY5, and JS09GY6) of avian leukosis virus subgroup J (ALV-J) were isolated from six infected commercial layer flocks displaying both hemangioma and myeloid leukosis (ML), which shared the same parental line, in China in 2009. 相似文献2.
3.
Several subgroup J-like avian leukosis viruses (ALV-Js) were isolated from broiler breeder (BB) and commercial broiler flocks experiencing myeloid leukosis (ML) at 4 wk of age or older. In all cases, diagnosis of ML was based on the presence of typical gross and microscopic lesions in affected tissues. The isolates were classified as ALV-J by 1) their ability to propagate in chicken embryo fibroblasts (CEF) that are resistant to avian leukosis virus (ALV) subgroups A and E (C/AE) and 2) positive reaction in a polymerase chain reaction with primers specific for ALV-J. The prototype strain of these isolates, an isolate termed ADOL-Hc1, was obtained from an adult BB flock that had a history of ML. The ADOL-Hc1 was isolated and propagated on C/AE CEF and was distinct antigenically from ALV of subgroups A, B, C, D, and E, as determined by virus neutralization tests. Antibody to ADOL-Hc1 neutralized strain HPRS-103, the prototype of ALV-J isolated from meat-type chickens in the United Kingdom, but antibody to HPRS-103 did not neutralize strain ADOL-Hc1. On the basis of both viremia and antibody, prevalence of ALV-J infection in affected flocks was as high as 87%. Viremia in day-old chicks of three different hatches from a BB flock naturally infected with ALV-J varied from 4% to 25%; in two of the three hatches, 100% of chicks that tested negative for virus at hatch had evidence of viremia by 8 wk of age. The data document the isolation of ALV-J from meat-type chickens experiencing ML as young as 4 wk of age. The data also suggest that strain ADOL-Hc1 is antigenically related, but not identical, to strain HPRS-103 and that contact transmission of ALV-J is efficient and can lead to tolerant infection. 相似文献
4.
Infection of broiler chickens with subgroup J avian leukosis virus (ALV) results in the induction of myeloid tumors. However, although egg-type chickens are susceptible to infection with ALV-J, the tumor incidence is very low, and on rare occasions the tumors observed are of the myeloid lineage. We recently described the isolation of an ALV (AF115-4) from commercial egg-type chickens suffering from myeloid leukosis. AF115-4 was initially identified as an ALV-J isolate based on PCR analysis of the long terminal repeat (LTR). However, further characterization of the viral envelope indicated that the virus is recombinant with subgroups B envelope and J LTR. Here we further characterize this recombinant virus at both the molecular and biological levels. We show that the AF115-4 isolate expresses a recombinant envelope glycoprotein encoded by a subgroup B gp85 region and a subgroup E gp37 region. The host range ofAF115-4 was analyzed using cells resistant to infection by subgroups A/B, J, or E; this shows that no ALV-J was present in the isolates obtained from the affected chickens. Additional antigenic characterization of AF115-4 using chicken sera specific for subgroups B or J indicated that no ALV-J was present in the samples examined. Inoculation of AF 115-4 into ALV-susceptible 1515 X 71 chickens resulted in the induction of lymphoid leukosis but not the expected myeloid leukosis affecting the commercial chickens. These results suggest that differences in the genetic makeup of the chickens from which AF115-4 was isolated and the line 1515 X 71 used in the present experiments may be responsible for the observed differences in pathogenicity. In addition, the results suggest that ALV-J continues to evolve by recombination, generating new viruses with different pathological properties. 相似文献
5.
血管瘤相关J亚群禽白血病病毒ZH-08株的分离与全基因组序列测定 总被引：3，自引：0，他引：3
本研究从临床表现为典型血管瘤型禽白血病病例的广东某肉种鸡场的病鸡中,分离到1株J亚群禽白血病病毒(ALV-J),命名为ZH-08。利用ELISA抗原检测、PCR和间接免疫荧光试验对分离株进行鉴定,结果都呈阳性。依据ALV-J原型株HPRS-103前病毒全基因组序列设计并合成3对引物,采用分段扩增的方法完成了分离株的全基因组序列测定。结果显示该分离株基因组序列全长7 597 bp,与已公开的全基因组序列大小比较略有差异,但符合典型的复制完全型反转录病毒的基因组结构,基因序列中不含已知致癌基因。将该分离株的亚群特异性gp85基因序列与国内外各参考株相应序列进行相似性比较,发现ZH-08与YZ9901株相似性最高(93.7%)。基于gp85核苷酸序列的系统进化分析表明:ZH-08株与SD07LK1株的亲缘关系最近。本研究为该毒株的生物学特性以及致病机制研究奠定了基础。 相似文献
6.
为了解蛋鸡中禽白血病当前流行毒株类型及其gp85基因分子特征,在临床症状和病理组织学观察的基础上,采用ELISA抗原检测、特异性PCR以及亚群特异性免疫荧光试验(IFA),证实从黑龙江省某鸡场送检蛋鸡中分离到1株J亚群禽白血病病毒(ALV-J),命名为HLJ09 MDJ-1;克隆了其gp85基因,并进行了序列分析比较。结果表明,该分离株的gp85基因与国内外其他ALV-J毒株的核苷酸同源性为88.2%～98.2%。其中,HLJ09 MDJ-1与英国原型株HPRS-103的同源性最高(98.2%),与美国株ADOL-7501的同源性最低(88.2%),表明HLJ09 MDJ-1的gp85基因与HPRS-103有较近的亲缘关系。本研究从蛋鸡中分离到ALV-J毒株,为研究ALV-J宿主嗜性改变和ALV-J对蛋鸡致病机制提供了素材。 相似文献
7.
用ALV-J gp85单克隆抗体证明蛋鸡存在J亚群禽白血病 总被引：20，自引：1，他引：20
采用免疫组化法，对病理学初步诊断为蛋用型鸡J亚群白血病的自然发病鸡的肿瘤、骨髓、肝脏、脾脏、肾脏、肺脏、心脏、胰脏、输卵管、卵巢、腺胃、骨骼肌、大脑、坐骨神经，用特异性抗J亚群禽白血病病毒(ALV—J)囊膜糖蛋白gp85的单克隆抗体进行检测，待检的组织切片中均检出阳性抗原，免疫组化的研究结果与病理学诊断结果相一致。在国内外首次发现并报道蛋用型鸡J亚群禽白血病的自然病例。 相似文献
8.
将血管瘤病变型相关的J亚型禽白血病病毒(ALV-J)SCAU-HN06株通过尿囊腔途径接种黄鸡11日龄鸡胚,对其出壳后不同时间点(1、2、3、4、5、6、7、8、9、10、12、14、16、18、20周)的病毒血症、ALV-J特异性抗体及泄殖腔拭子p27抗原分别进行了动态检测。试验结果表明,SCAU-HN06组在1周即检测到病毒血症,7周时有一个高峰,9周后病毒血症阳性率始终维持在80%以上;从6周时开始检测到ALV-J特异性抗体,但监测过程中抗体阳性率最高仅为33.3%(18周),其余均在22%以下;泄殖腔p27抗原阳性率在前3周迅速升高,3周时达到顶峰,随后维持较高的阳性率。由研究结果可知,经鸡胚接种ALV-J的感染黄鸡可导致持续性病毒血症,并容易产生免疫耐受;这项研究可为黄鸡ALV-J的防控与净化提供科学依据。 相似文献
9.
商品蛋鸡成髓细胞瘤、血管瘤型J亚群白血病病毒特性的研究 总被引：5，自引：0，他引：5
2007年7月山东某蛋鸡场150日龄海兰褐蛋鸡发病,前期经病理学和免疫组织化学检测证明和ALV—J密切相关。取4只病鸡的肝组织处理后接种鸡胚成纤维细胞（CEF）培养,用ALV—J单抗G2-3进行间接免疫荧光检测,结果呈现强阳性,将反应为阳性的4株病毒分别命名为WS0701、WS0702、WS0703和WS0704;根据ALV-J原型株HPRS-103的序列设计1对针对外源性ALV—J的引物P1和P2,提取阳性CEF基因组DNA作为模板,PCR扩增后,得到长度为924bp的片段;PCR扩增产物测序结果显示,与HPRS-103的同源性为95．0％～97．5％,与国内分离株的同源性为92．0％～95．9％;遗传变异分析结果表明成髓细胞瘤型、血管瘤型ALV-J与国内毒株的同源性较远,而与HPRS-103的同源性最近,这说明ALV—J在蛋鸡体内复制的过程中有返祖现象,并呈现了新的肿瘤学特征。 相似文献
10.
两个规模化商品蛋鸡场发病鸡群临床解剖显示：脚部和翅膀有血管瘤、肌肉组织内出现纤维肉瘤,肿大的肝、脾出现髓细胞瘤;经组织病理学观察符合J亚群禽白血病（ALV-J）的病理学特征。针对ALV—J保守序列P27基因设计特异引物,建立PCR方法,从出现病理改变的各组织脏器、各多发性肿瘤中以及血液中均检测到ALV-J,证实该病为以髓细胞瘤,血管瘤和纤维肉瘤为主的多发性肿瘤传染病。对扩增产物序列进行测定后,与原型株序列进行比较,核苷酸同源性为98．27％,从分子水平上证明为J亚群禽白血病,用PCR检测进一步验证J亚群禽白血病毒的感染机制和引起鸡群的病理改变。 相似文献
11.
J亚群禽白血病多发性肿瘤的病理改变与PCR检测 总被引：1，自引：0，他引：1
两个规模化商品蛋鸡场发病鸡群临床解剖显示:脚部和翅膀有血管瘤、肌肉组织内出现纤维肉瘤,肿大的肝、脾出现髓细胞瘤;经组织病理学观察符合J亚群禽白血病(ALV-J)的病理学特征。针对ALV-J保守序列P27基因设计特异引物,建立PCR方法,从出现病理改变的各组织脏器、各多发性肿瘤中以及血液中均检测到ALV-J,证实该病为以髓细胞瘤、血管瘤和纤维肉瘤为主的多发性肿瘤传染病。对扩增产物序列进行测定后,与原型株序列进行比较,核苷酸同源性为98.27%,从分子水平上证明为J亚群禽白血病,用PCR检测进一步验证J亚群禽白血病毒的感染机制和引起鸡群的病理改变。 相似文献
12.
为分析我国J亚群禽白血病病毒(ALV-J)蛋鸡分离株的进化关系,本研究将山东省某鸡场采集的蛋鸡病料样品接种DF-1细胞系,利用ELISA群特异性抗原检测以及亚群特异性间接免疫荧光方法,分离鉴定得到一株ALV-J,命名为SD1009,并对其进行全基因组测序,将该序列与其他ALV-J代表性病毒株序列进行比较。结果表明:SD1009分离株的gag和pol基因相对保守,与各参考病毒株的同源性为95%～99%,env基因的同源性为91%～95%;在其5'UTR中出现了连续19 bp的插入突变,与TW-3577、SDAU09C3、JS09GY6、JS09GY3蛋鸡分离株的5'UTR基本一致,提示19 bp的插入现象可能是近年来蛋鸡ALV-J的进化趋势;此外,其3'UTR的rTM和DR区出现部分缺失现象,该缺失部分也可能与ALV-J进化相关。 相似文献
13.
J亚群与E亚群禽白血病自然重组病毒的全基因组序列分析 总被引：2，自引：1，他引：1
为了解我国东北地区部分养鸡场禽白血病病毒(ALV)的基因组序列特征及其变异情况,本研究从具有典型血管瘤病变的禽白血病发病鸡中分离到一株J亚群ALV(ALV-J)命名为JL0901,并进行了全基因测序.将该序列与已发表的ALV-J毒株序列进行比较研究,结果表明JL0901基因组的gag和pol基因相对保守,而env基因和3'端非编码区(3'UTR)的变异较大.对JL0901的env基因核苷酸序列进一步分析发现,在其gp85基因和gp37基因交界位置发生J亚群和E亚群ALV重组现象.本研究证实国内鸡群中存在J亚群和其他亚群ALV的自然重组现象,并表明国内ALV已出现新的变异趋势. 相似文献
14.
从安徽省的黄羽肉鸡和罗曼蛋鸡中各分离鉴定出1株J亚群禽白血病病毒,克隆获得了2条相应的gp85基因序列,并与参考毒株进行序列比对。结果表明,两分离毒株与J亚群参考毒株同源性为82.1%~99.4%,分离毒株之间同源性为85.4%。其中肉鸡分离毒株与J亚群原型毒株HPRS-103同源性为97.1%,与J亚群国内毒株SD09TA04、SDYC02J同源性均为99.4%;蛋鸡分离毒株与HPRS-103的同源性为89.0%,与SD09TA04和SDYC02J同源性仅为88.6%。两分离毒株的gp85氨基酸序列出现突变和缺失,在高变区hr1、hr2变异明显。进化分析进一步表明,2个分离毒株亲缘关系较远,可能来源于不同的原始病毒株。 相似文献
15.
Avian leukosis virus subgroup J (ALV-J) can cause a variety of neoplasms, including mainly myeloid leukosis (myelocytomatosis) and nephromas. Other tumours, such as histiocytic sarcoma (HS), haemangiosarcoma and mesothelioma, may also develop. In a previous article we described a case in which myeloid leukosis, haemangiomas and leiomyosarcomas appeared simultaneously in a commercial layer flock with infection by ALV-J. The present research was completed to understand the molecular characteristics of the ALV-J strain that induced clinical myeloid leukosis, haemangiomas and leiomyosarcomas. Two strains of ALV-J (SDAU1001 and SDAU1002) were isolated and identified, and their full-length sequences were analysed. The complete genome nucleotide sequences of these two isolates were different in length, 7652 nt and 7636 nt, respectively. They shared 98.9% identity with each other, and 93.4% to 97.8% nucleotide identity to the reference ALV-J isolates. A 19-nucleotide repeat sequence was identified in the primer binding site (PBS) leader region of isolate SDAU1001. A base substitution mutation (base 15 C-T) in this insertion was identified. However, the identical insertion at the same site was not found in SDAU1002. The gag and pol genes of the two viruses were more conserved than the env gene. One key deletion in the E element was a common feature of SDAU1001 and SDAU1002. SDAU1001 and SDAU1002, possibly recombinants of ALV-J and another avian retrovirus, may share the same ancestor. Co-infection by SDAU1001 and SDAU1002 isolates is a possible explanation why myeloid leukosis, haemangiomas, and leiomyosarcomas appeared simultaneously in the same commercial layer flock. 相似文献
16.
Since a new envelope subgroup (J) of the avian leukosis-sarcomatosis-complex was isolated for the first time from broiler breeders in the United Kingdom in 1989 and was characterized and associated with myeloid leukosis (syn. myelocytomatosis) the emergence of this subgroup was reported from all over the world. Thus the first known case of subgroup J avian leukosis in Switzerland in four imported broiler breeder flocks will be described. A total of 53 broiler breeder birds from four flocks showing reduced performance and increased mortality were submitted for postmortem examination. Approximately 20 blood samples from each flock were monitored serologically for antibodies against avian leukosis virus subgroup J (ALV-J). On necropsy myeloid leukosis (ML) was diagnosed in all four flocks. Furthermore the blood samples of three flocks showed significant ELISA-titres for ALV-J. 相似文献
17.
为了解J亚群禽白血病在黄羽肉鸡中的流行状况,采用病料研磨液接种DF-1细胞、ELISA p27抗原检测、PCR扩增等方法,从广东某鸡场送检疑似禽白血病的黄羽肉鸡病料中分离鉴定出1株J亚群禽白血病病毒,命名为GDLZ0715。为进一步了解该病毒分子学特性,对其进行全基因组测序,并与其他ALV-J毒株进行比较。结果表明,GDLZ0715分离株整个基因组中gag、pol、env基因和LTR相对保守,与各参考ALV-J毒株序列同源性分别达93.5%~95.9%、96.8%~97.3%、89.6%~94.6%和90.8%~95.1%;3′UTR变异较大,与各ALV-J参考毒株序列同源性仅为80.5%~93.4%,其中rTM和E元件大量碱基缺失;进一步分析表明3′UTR中rTM区和E元件大量碱基缺失正成为我国肉鸡ALV-J毒株的变异趋势。 相似文献
18.
三黄鸡临床多发性肿瘤相关的J亚群禽白血病病毒的分离鉴定及其病理学观察 总被引：1，自引：0，他引：1
为研究蛋鸡多发性肿瘤的病因,本实验对病鸡肿瘤组织进行病毒分离、培养及PCR检测,均扩增出针对J亚群禽白血病病毒(ALV-J)gp85基因序列的阳性条带;组织病理学观察显示发病鸡呈现髓细胞瘤、血管瘤以及纤维肉瘤等多发性肿瘤的病理变化;肿瘤细胞通过血液转移、浸润,在肝和脾组织形成局灶性或弥漫性肿瘤病灶。免疫组化染色显示肿瘤组织内,部分肿瘤细胞呈现阳性反应,表明只有部分肿瘤细胞存在ALV-J感染,而大部分肿瘤细胞检测结果呈阴性。这些病毒检测为阴性的肿瘤细胞可能是正常细胞转化为肿瘤细胞大量克隆化增殖的结果。 相似文献
19.
表现腺胃炎的蛋用型鸡J亚群-白血病病毒的分离与鉴定 总被引：2，自引：1，他引：1
从表现腺胃炎的尼克珊瑚粉商品代蛋鸡中分离到J亚群-白血病病毒(ALV-J)。将病料或鸡白细胞接种于CEF,培养12 d,分别采用单克隆抗体间接免疫荧光试验检测,结果10只鸡中有9只鸡分离到ALV-J,其中有4只鸡还存在与禽网状内皮增生病病毒(REV)的共感染。通过PCR扩增gp85基因,与已发表的20株ALV-J进行同源性比较。结果表明,与来自白羽肉鸡的HPRS103的同源性为97.8%,而与来自蛋用型鸡的SD07LK1株的同源性为93.0%。本研究发现,在某些仅仅发生腺胃炎的鸡也可能普遍存在ALV-J感染,再次显示了腺胃炎病料中病毒感染的多样性。ALV-J可能成为致腺胃炎的病原之一,但其致病作用有待进一步研究。 相似文献
20.
对江苏商品蛋鸡6个鸡场临床表现严重血管瘤鸡群的送检样品进行了病理学分析,结果发现,送检的6只商品蛋鸡均可见体表血管瘤,内脏主要在肝、脾、肌胃和肠系膜表面的大小不等的血管瘤,引起严重的肝破裂、脾脏失血和肌胃失血,6个病例中有4个病例出现血管瘤和髓样细胞瘤并存。追踪检测父母代蛋鸡表明,父母代蛋鸡场的各个日龄鸡群均存在不同程度的丁亚群禽白血病病毒（Avian Leukosis Vivus SubgroupJ,ALV-J感染;而送检商品病鸡均呈现病毒血症而抗体阴性,提示垂直传播的可能;ALV-J特异性RT-PCR结果显示,6个样品均存在ALV-J感染;对扩增克隆的部分序列分析可见,此六株病毒之间同源性为97．9％～99．6％,而与原型毒HPRS103之间的同源性则较低（94．6％～95．2％）,与同为蛋鸡分离毒株AY360088和SD07LK1同源性为94．2％～95．0％。 相似文献