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根据鸭副黏病毒(DPMV)和鸭圆环病毒(DuCV)保守基因序列,设计了2对针对鸭副黏病毒和鸭圆环病毒的特异性引物和2条不同荧光基团标记的TaqMan探针,建立了鸭副黏病毒和鸭圆环病毒的二重荧光定量PCR检测方法。该方法敏感性好,对鸭副黏病毒和鸭圆环病毒的检测敏感性分别达到160和140个拷贝数;该方法特异性强,对鸭肝炎病毒、番鸭细小病毒、鸭瘟病毒和H9型禽流感病毒等病原体的检测全为阴性;应用该方法对118份临床病料进行检测,结果检出鸭副黏病毒和鸭圆环病毒阳性感染率分别为0.85%和8.47%,无混合感染。本试验建立的二重荧光定量PCR具有快速、特异、敏感和重复性好等优点,适用于鸭副黏病毒和鸭圆环病毒的快速诊断和监测。 相似文献
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本研究根据GenBank中鸭新城疫病毒(NDV)的F基因和鸭圆环病毒(DuCV)的V1/rep基因的保守序列,各设计一对特异性引物,并对二重PCR的扩增条件进行优化,建立了鸭NDV和DuCV的二重PCR检测方法。对混合样品进行扩增,得到2条大小为493bp(鸭NDV)和218bp(DuCV)的特异性条带,与预扩增片段相符。而对番鸭细小病毒、鸭瘟病毒、鸭肝炎病毒、鸭源小鹅瘟病毒、鸭H9亚型流感病毒、鸭疫里氏杆菌、大肠杆菌、禽多杀性巴氏杆菌等病原检测,结果为阴性。该方法的敏感性试验表明,鸭NDV的核酸最小量为40fg,DuCV为20fg。 相似文献
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鸭病毒性肝炎(DVH)是雏鸭的一种以肝炎为主要特征的急性病毒传染病。自Lcvin等于1945年春季观察到鸭雏的一种急性传染病,并于1950年首先在鸭胚中分离出鸭肝炎病毒(DHV)以来,世界许多国家先后报道了此病。我国黄均健(1963)最早报道在上海某些鸭场曾发生过DVH,随后许多省市也相继报道了本病的发生。1989年黑龙江省某县的两个养鸭场发生雏鸭大批死亡之后,来我所求诊。经笔者初步诊断为雏鸭病毒性肝炎。现报告如下。一、流行病学调查这两个养鸭场于1989年8月分别由某地引进樱桃谷鸭一日龄鸭雏1500只和1700 相似文献
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番鸭细小病毒与鸭圆环病毒二重PCR方法的建立 总被引：1，自引：0，他引：1
根据基因库中鸭圆环病毒和番鸭细小病毒的基因序列,分别设计了两对特异性引物,通过对二重PCR扩增条件的优化,研究建立了可同时鉴别检测鸭圆环病毒和番鸭细小病毒的二重PCR方法。用该方法对同一样品中鸭圆环病毒和番鸭细小病毒的模板进行PCR扩增,结果均得到了与实验设计相符的351bp（鸭圆环病毒）和474bp（番鸭细小病毒）的扩增条带,而对鸭Ⅰ型肝炎病毒、鹅细小病毒、鸭副黏病毒、鸭瘟病毒和禽流感病毒等病原体的检测全为阴性。敏感性测定结果表明：该二重PCR技术最低能检出100fg的鸭圆环病毒和番鸭细小病毒DNA模板。研究建立的鸭圆环病毒和番鸭细小病毒的二重PCR方法,具有快速、敏感、特异、定量和重复性好等优点,可用于临床上鸭圆环病毒和番鸭细小病毒感染的检测。 相似文献
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１９９１年从云南某地疑似鸭病毒性肝炎（ＤＶＨ）的病雏鸭肝脏分离到一株病毒，经鸡胚染试验，电镜形态观察，中和试验鉴定，证明为鸭病毒性肝炎病毒１型（ＤＨＶ－Ｉ）毒株。该病毒对鸡胚高度适应，传递至８０代对雏鸭无致病力，经易感雏鸭连传代毒力无反强现象，故命名为ＤＨＶ－ＫＭ６０株。鸡胚测试其滴度为ＬＤ５０１０６－７．３２／０．２ｍｌ。采用ＤＨＶ－ＫＭ６０制备疫苗，经口服免疫，对１日龄雏鸭的安全性高，免疫原性 相似文献
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鸭病毒性肝炎是由鸭肝炎病毒引起雏鸭的一种高死亡率传染病。为探究该病的致病机理，本实验用患鸭病毒性肝炎死亡的鸭肝脏制成病毒组织液，并用该组织液人工感染3日龄健康雏鸭。对感染雏鸭的临床症状、发病情况进行观察，并测定了部分健康雏鸭和濒临死亡时的感染雏鸭的血糖、谷草转氨酶、谷丙转氨酶等13项血液生化指标。结果表明：血液葡萄糖、血液淀粉酶、胆碱脂酶含量在病发后显著下降；谷草转氨酶、谷丙转氨酶、肌酐、尿酸、碱性磷酸酶的含量显著升高；总蛋白、白蛋白、球蛋白含量在病发时略微下降。上述的试验结果提示：雏鸭患鸭病毒性肝炎后肝脏受损最明显，同时胰腺、肾脏也受损严重。 相似文献
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Diagnosis of duck plague in waterfowl by polymerase chain reaction 总被引：19，自引：0，他引：19
A recently developed polymerase chain reaction (PCR) assay was used for diagnosis of duck plague in waterfowl tissues from past and current cases of waterfowl mortality and to identify duck plague virus in combined cloacal/oral-pharyngeal swab samples from healthy mallards (Anas platyrhynchos) after a disease outbreak. The PCR was able to detect viral DNA from all the individual or pooled tissues assayed from 10 waterfowl, including liver and spleen samples from three Muscovy ducks (Cairina moschata domesticus) that did not yield virus isolates. The strong staining intensity of the PCR products from the waterfowl tissues indicated that large amounts of virus were present, even when virus was not isolated. Duck plague DNA was also detected in a cloacal swab sample from a wood duck (Aix sponsa) carcass submitted for diagnosis. The PCR assay identified duck plague DNA in 13 swab samples that produced virus isolates from carrier mallards sampled in 1981 after a duck plague die-off. The duck plague PCR clearly demonstrated the ability to quickly diagnose duck plague in suspect mortality cases and to detect virus shed by carrier waterfowl. 相似文献
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Surviving birds from nine duck plague outbreaks in urban and confined waterfowl were sampled for duck plague (DP) virus and DP antibody during 1979-86. Duck plague virus was found in combined oral and cloacal swabs of birds from three outbreaks, and DP-neutralizing antibody was demonstrated in some birds from all nine outbreaks. Greater prevalence of DP antibody and higher titers were found in survivors from confined populations than from free-flying urban populations. Free-flying waterfowl from within 52 km of four DP outbreak sites were also sampled; virus was not found in any birds, but DP antibody was found in urban waterfowl in the vicinity of an outbreak in Potterville, Michigan. No evidence of exposure to or shedding of DP virus in migratory waterfowl was found in two regions where DP appears enzootic in urban and confined waterfowl (Eastern Shore of Maryland and the vicinity of Sacramento, California). 相似文献
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国家水禽基因库七个家鸭群体遗传多样性检测 总被引：5，自引：2，他引：3
试验通过微卫星标记对国家水禽种质资源基因库保存的7个家鸭保种群体的保种效果进行了遗传检测。结果表明:28个微卫星位点中有25个高度多态位点,可作为有效遗传标记用于各群体的遗传检测。7个鸭群体中,平均杂和度范围是0.5137～0.6035,最高的是荆江麻鸭,最低的是山麻鸭,反映了各鸭群体保持了较丰富的遗传多样性。Ds遗传距离表明各品种间的遗传距离较远,巢湖鸭与金定鸭之间最远,为0.8835;金定鸭与山麻鸭最近,为0.2674。7个家鸭群体间的Ds值都比较大,说明各群体间的基因交流较少。上述结果反映了在国家水禽种质资源基因库所保存的7个地方鸭群体的保种方法良好,保种效果有效。 相似文献
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A Hlinak K Ziedler R Heiss 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1992,39(9):641-648
8410 samples from Moscovy duck, Pekin duck and geese were incorporated into examinations of antibodies against the Newcastle Disease virus. A new enzyme-immuno-assay (EIA) for antibody detection in Moscovy duck and Pekin duck was developed using purified antigen from NDV-strain "La Sota". The epidemiology as well as the relation of incidence of the Newcastle Disease in waterfowl was discussed. 相似文献
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Coliform organisms were the principal contaminants of rotten eggs from commercial duck hatcheries and incubated eggs of 45 species of waterfowl. Micrococci were the main contaminants of “incubator clears” obtained from a chicken hatchery. Pseudomonas, Acinetobacter and Bacillus spp were minor contaminants of the eggs of waterfowl. All the coliforms recovered from waterfowl eggs formed a large, well‐defined capsule when grown on nutrient agar containing glucose or lactose. 相似文献
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R E Gough 《The Veterinary record》1984,114(11):262-265
From 1977 to 1983 the Central Veterinary Laboratory, Weybridge confirmed 19 outbreaks of duck virus enteritis in the United Kingdom. All the outbreaks involved collections of captive waterfowl and there were no reported cases in commercial ducks. In many instances the disease was associated with contact with migrating waterfowl, particularly male mallards (Anas platyrhynchos). Muscovy ducks (Cairina moschata) and related species appeared to be particularly susceptible. The most sensitive system for isolating the virus was muscovy duck embryo tissue cultures. The duckling inoculation test was found to be the most reliable method of confirming the disease. 相似文献
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Free-living waterfowl residing in metropolitan parks in central Ohio were surveyed for the fecal shedding and antimicrobial susceptibility patterns of Campylobacter jejuni, Escherichia coli, Salmonella spp., and Pasteurella multocida. In addition, a survey for intestinal parasites was also conducted in these same waterfowl to determine parasite burdens in free-living waterfowl. Prevalences of 67%, 50%, and 0.2% of E. coli, C. jejuni, and Salmonella spp., respectively, were observed for all waterfowl species. Pasteurella multocida was not isolated from the sampled population. Salmonella java was isolated from one mallard duck. Statistically, there was a significantly higher E. coli isolation rate for mallard ducks than for Canada geese, but no difference was observed for C. jejuni isolation rates between waterfowl species. Antimicrobial susceptibility testing was conducted via the disk diffusion method and multidrug resistance was exhibited for penicillin G, lincomycin, vancomycin, erythromycin, and bacitracin. In addition, the prevalence of endoparasites in these sampled waterfowl ranged from 5% to 66%. Protozoan oocysts were most prevalent followed by nematode ova. No trematode or cestode ovum was recovered from this sampled population. 相似文献
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Nadia T. Rubio-Cisneros Octavio Aburto-Oropeza Jason Murray Charlotte E. Gonzalez-Abraham Jeremy Jackson Exequiel Ezcurra 《Human Dimensions of Wildlife》2014,19(1):1-16
This article explores transnational ecosystem services in North America, provided by winter habitat for waterfowl in western Mexico coastal lagoons, and the hunting industry supported by these birds in the United States. This article shows that the number of waterfowl harvested in the United States is related to the abundance of waterfowl wintering in Mexico. On average, this flow of ecosystem services annually yields US$ 4.68 million in hunting stamp sales in the western United States. A demand curve, fitted to duck hunting licenses as a function of stamp price and previous-year waterfowl harvest, estimated US$3–6 million in consumer surplus produced in addition to governmental stamp sales revenue. This strongly suggests that waterfowl wintering habitat in western Mexico is economically valuable to U.S. hunters. Because hunters may benefit substantially from these habitats they may be willing to pay for conservation efforts in western Mexico that can result in transnational benefits received in the United States. 相似文献
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Isolation of an apathogenic immunogenic strain of duck enteritis virus from waterfowl in California 总被引：2，自引：0，他引：2
A herpesvirus isolated from waterfowl dying of duck enteritis (DE) was tentatively designated the Sheridan-83. It was serologically related to the original Holland and Lake Andes (LA) strains of duck enteritis viruses (DEV). Other biological characteristics indicated that the Sheridan-83 was more closely related to the Holland strain than to the LA virus. The Sheridan-83 was nonpathogenic to ducks, and ducks inoculated with this virus developed resistance to challenge with the virulent strain LA. 相似文献