共查询到20条相似文献，搜索用时 62 毫秒
1.
为了深入了解滨麦（Leymus mollis）HMWGS基因的结构特征,采用PCR方法,从滨麦基因组中分离出HMWGS基因启动子序列（Genbank登录号：FJ600498）和编码区序列（Genbank登录号：GQ169797）。启动子序列（FJ600498）从5′至3′方向依次有Ebox、Nbox、Gbox、HMW谷蛋白特异增强子和TATAbox等麦谷蛋白特异调控元件,推断其为滨麦HMWGS启动子基因。编码区序列（GQ169797）具有单一完整的开放阅读框（ORF）,编码396个氨基酸残基,依次包含信号肽、N末端区、中部重复区和C末端区等HMWGS的典型结构区域;中部重复区主要重复单元为6肽（PQQGQQ）和9肽（GYYPTSPQQ）;有6个半胱氨酸残基（Cys）,分布在N末端区（5个）和C末端区（1个）,第3、4个相邻。推断其为滨麦的y型HMWGS编码区基因。系统进化分析表明,启动子序列（FJ600498）与异形花草（He. piliferum）和冰草（Ag. cristatum）的HMWGS启动子基因序列具有相对较近的同源关系;编码区序列（GQ169797）与纤毛鹅观草（Elymus ciliaris）和披碱草（E. glaucus）的HMWGS编码区基因序列具有相对较近的同源关系。 相似文献
2.
为了挖掘和有效利用陕253中的优良基因,根据LMW-GS编码基因5′和3′端的保守序列设计引物,采用PCR方法以陕253的基因组DNA为模板进行扩增,分别回收并克隆到载体上,测序后得到11个新LMW-GS基因。GenBank登录号分别为GQ892576~GQ892580和GQ892583~GQ892588,其长度为903~1 081 bp。其中,GQ892576、GQ892585、GQ892586、GQ892587和GQ892588具有完整编码区,可分别编码305、351、298、351和307个氨基酸残基的成熟蛋白。而GQ892577、GQ892578、GQ892579、GQ892580、GQ892583和GQ892584由于在编码区内出现提前终止密码子,推测为假基因。推导的氨基酸序列结果显示:它们都具有LMW-m型低分子量谷蛋白亚基的典型结构特征。 相似文献
3.
为探讨山茶重瓣花形成的机理，在山茶A和B类基因研究的基础上，通过同源基因查找分析，从山茶（Camellia japonica）重瓣品种‘金盘荔枝’（‘Jinpan Lizhi’）早期花芽中克隆了长度为1 418 bp的C类基因CjAG1全长cDNA序列（Genbank登录号JX843816），包括长度为224 bp的5′非编码区、768 bp的编码区和426 bp的3′非编码区三部分。基因编码的蛋白质包含258个氨基酸残基，属于不稳定亲水性蛋白，α-螺旋和无规则卷曲构成了其结构骨架。Real-time P 相似文献
4.
为了克隆节节麦中的HMW-GS基因,利用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)分析了节节麦的HMW-GS,发现1种编码序列未知的y-型亚基,即Dy8.1t亚基。通过对目的片段的AS-PCR扩增、克隆、测序和氨基酸序列推导,发现这种未知序列具有典型HMW-GS的序列结构特征。通过与已知亚基序列比较发现,Dy8.1t与Dy10t、Dy10.4t、Dy10.5t和Dy12t在N-端序列有一个氨基酸差别(R-G)。与已知氨基酸序列的HMW-GS多序列比对和系统进化关系分析,证实Dy8.1t亚基是D基因组编码的高分子量谷蛋白y-型亚基家族的新成员。 相似文献
5.
为揭示Glu-D3位点编码的低分子量谷蛋白亚基（LMW-GS）基因的分子特征,并开发能有效地应于育种选择的分子标记,根据已注册的D基因组来源的LMW-GS基因编码区的保守区域,设计1对特异性简并引物,采用基因组PCR法,从豫麦50中克隆出LMW-GS基因后进行序列分析。结果表明,本研究共得到了6个具有独特编码区的核苷酸序列(命名为LMW-Y50-1~LMW-Y50-6;GenBank注册号为JN831414~JN831417、HM055908和JX828375)。其中,4个（LMW-Y50-2,LMW-Y50-4~LMW-Y50-6）具有完整的开放阅读框。推导的氨基酸序列比对分析表明,这4个基因均具有LMW-m型LMW-GS的典型分子特征,其中,LMW-Y50-5缺失N-末端第1个保守的半胱氨酸残基,LMW-Y50-6在C-末端I区含有1个额外的半胱氨酸残基,LMW-Y50-2和LMW-Y50-4均含有8个保守的半胱氨酸残基。根据N-末端和C-末端保守序列,4个基因均属于定位在1D染色体上的IV型7、8和10组基因。所得基因序列与36个代表不同位点、不同等位变异或不同单倍型LMW-GS基因序列的聚类分析表明,4个基因分别与D3-2、D3-4和D3-6单倍型有更高的同源性。表明本研究设计的简并引物可对D基因组来源的m-型LMW-GS基因进行特异扩增。而考虑到半胱氨酸残基数目和位置对面筋品质的重要影响,推测含有异常半胱氨酸残基数目的LMW-Y50-3和LMW-Y50-4基因,尤其是LMW-Y50-3基因,可能与豫麦50优质弱筋的面筋品质有关。 相似文献
6.
豫麦34低分子量谷蛋白亚基一个新基因的克隆 总被引：1，自引：0，他引：1
为了解强筋优质小麦品种豫麦34的低分子量谷蛋白亚基(LMW-GS)的基因构成,利用普通小麦LMW-GS基因特异引物,采用PCR扩增技术从中克隆得到一个LMW-GS新基因LMWY34(GenBank No.GU183486)。该基因具有LMW-GS基因的典型结构特征,编码区全长906 bp,编码302个氨基酸。推导氨基酸序列显示,LMWY34的编码产物N-端具有LMW-m型低分子量谷蛋白亚基的典型特征,与已报道的GluD3-4位点编码的LMW-GS基因序列有很高的一致性(98.68%)。 相似文献
7.
根据已发表的γ-TMT基因序列(GenBank AF104220),从拟南芥(Arabidopsis thaliana)的cDNA中分离得到γ-TMT基因,根据已发表的油菜(Brassica napus)种子贮藏蛋白基因特异表达的2S启动子(登录号J02798)序列,从油菜DNA中分离得到2S启动子。为了同时提高花生中维生素E和油酸含量,构建得到了双价种子特异表达载体pCAMBIA1300AT,它带有FAD2基因反义RNA干扰体和γ-TMT基因的种子特异表达单元。该载体已登录在GenBank上(登录号FJ362601)。通过农杆菌C58介导转化花生品种闽花6号,获得PCR阳性的转化植株。 相似文献
8.
野生香蕉几丁质酶基因的克隆与序列分析 总被引：1，自引：0，他引：1
以野生香蕉叶片为试材,采用RT-PCR结合RACE法,通过T/A克隆测序,获得野生香蕉几丁质酶基因全长序列,并在GenBank登录(登录号为:FJ858155)。野生香蕉几丁质酶基因(ChiⅠ1)全长1115bp,包含924bp的ORF、14bp的5'非编码区、177bp的3'非编码区及17bp3'poly(A)尾。该基因开放阅读框编码307个氨基酸,分子量为32424.1u,预测的理论等电点为6.28,其结构包括信号肽、几丁质结合区、糖苷水解酶区。蛋白结构分析结果表明,该基因编码蛋白为跨膜蛋白,存在于胞外。野生香蕉几丁质酶基因(ChiⅠ1)属于酸性几丁质酶classⅠb,与大蕉几丁质酶classⅠb相似性仅为59.4%。结构分析结果表明,该基因可能具有几丁质酶/溶菌酶特点,且在过敏反应中起重要作用。 相似文献
9.
为了阐明青稞种子中B组醇溶蛋白合成的遗传调控机制,为分子改良大麦和小麦籽粒品质奠定基础,以青稞品种Z09和Z26的基因组DNA为模板,根据已克隆的青稞B组醇溶蛋白(B-hordein)基因的5'端序列设计三个基因特异的反向引物,分别与随机简并引物配对,进行热不对称嵌套PCR(Thermal asymmetric interlaced PCR,TAIL-PCR)扩增,从两份西藏青稞材料中分离克隆出2个B-hordein基因的上游调控序列.序列测定结果表明,两个扩增片段长度分别为395和396 bp,加上已知B-hordein基因中的GSP2引物序列与翻译起始密码子之间198 bp的长度,则可得到约600 bp的B-hordein基因5'上游调控序列.将所得序列与Genbank中的三个贮藏蛋白基因的对应区段进行序列比对,所得序列与来自野生智利大麦(H.chilense)的B3-hordein基因(Genbank登录号:AY998010)、普通小麦(T.aestivum)的低分子量麦谷蛋白亚基基因(Genbank登录号:X07747)和栽培大麦(H.vulgare)的B-hordein基因(Genbank登录号:X03103)具有81%～95%的序列相似性.推测其TATA box位于-80 bp,CAAT-like box位于-140 bp处.另外,在-300 bp处存在一个胚乳盒(Endosperm Box,EB),包含EM基序和GCN4基序.EM基序高度保守,GCN4基序有一个核苷酸位点的突变.此外,在约-560 bp处存在一个胚乳盒类似结构. 相似文献
10.
采用电子克隆与实验克隆结合的方法获得了大豆对羟苯丙酮酸双加氧酶基因的cDNA序列,GenBank登录号为EF608178.利用多种生物信息学工具分析了该cDNA序列及其编码的蛋白质序列,分析了其启动子特征和电子表达谱.结果表明:该cDNA序列含有1个编码443个氨基酸的完整的开放读码框,3'端具有2个加尾信号和polyA尾巴.启动子区除含有通用核心元件外,还含有许多与光反应有关的作用元件;不同物种之间对羟苯丙酮酸双加氧酶的氨基酸序列同源性较高;该基因在光合作用和贮藏器官中的表达量高. 相似文献
11.
12.
Sorghum and millet phenols and antioxidants 总被引：5，自引：2，他引：5
Sorghum is a good source of phenolic compounds with a variety of genetically dependent types and levels including phenolic acids, flavonoids, and condensed tannins. Most sorghums do not contain condensed tannins, but all contain phenolic acids. Pigmented sorghums contain unique anthocyanins that could be potential food colorants. Some sorghums have a prominent pigmented testa that contains condensed tannins composed of flavan-3-ols with variable length. Flavan-3-ols of up to 8–10 units have been separated and quantitatively analyzed. These tannin sorghums are excellent antioxidants, which slow hydrolysis in foods, produce naturally dark-colored products and increase the dietary fiber levels of food products. Sorghums have high concentration of 3-deoxyanthocyanins (i.e. luteolinidin and apigenidin) that give stable pigments at high pH. Pigmented and tannin sorghum varieties have high antioxidant levels that are comparable to fruits and vegetables. Finger millet has tannins in some varieties that contain a red testa. There are limited data on the phenolic compounds in millets; only phenolic acids and flavones have been identified. 相似文献
13.
《Journal of Cereal Science》2007,45(3):236-251
Sorghum is a good source of phenolic compounds with a variety of genetically dependent types and levels including phenolic acids, flavonoids, and condensed tannins. Most sorghums do not contain condensed tannins, but all contain phenolic acids. Pigmented sorghums contain unique anthocyanins that could be potential food colorants. Some sorghums have a prominent pigmented testa that contains condensed tannins composed of flavan-3-ols with variable length. Flavan-3-ols of up to 8–10 units have been separated and quantitatively analyzed. These tannin sorghums are excellent antioxidants, which slow hydrolysis in foods, produce naturally dark-colored products and increase the dietary fiber levels of food products. Sorghums have high concentration of 3-deoxyanthocyanins (i.e. luteolinidin and apigenidin) that give stable pigments at high pH. Pigmented and tannin sorghum varieties have high antioxidant levels that are comparable to fruits and vegetables. Finger millet has tannins in some varieties that contain a red testa. There are limited data on the phenolic compounds in millets; only phenolic acids and flavones have been identified. 相似文献
14.
概述了海峡两岸农业交流与合作的现状,提出了应该共同呼吁尽快实现两岸“三通”,开放农业生物技术图书期刊资料交流以及开放生物学科方面网络的思路,以便为农业交流与合作的顺畅开展打好基础,并寄希望能够就糖蔗、果蔗以及甘蔗笋等方面的研究与开发到台湾学习取经,开展学术交流或与台湾同行共同开展研究,为我省甘蔗业的发展作出贡献。 相似文献
15.
种子加工、检验理论与技术现状及思考 总被引：3，自引：0，他引：3
种子加工及检验是种子向市场流通的关键，是商业化育种中赋予种子商品属性不可或缺的环节。种子加工、检验理论与技术的研究是完善种业产业链、实现规模化商业育种、夯实“育繁推一体化”种业科学的重要一环。中国的种业科学技术体系正在形成与完善之中，受制于行业发展水平，种子加工及检验理论与技术相对薄弱。本文回顾了种子加工及检验理论与技术研究发展历程，提出了完善中国种业科学发展的策略与建议。围绕种业发展的需要，在做好品种优质化繁育的基础上，分析种子加工及检验理论与技术的新方向和新需求，加强种业应用性研究，建立先进的种子质量检验体系，研制适合现代种业发展需求的种子加工设备，打造种子加工产业标准化生产体系，完善种业科学技术学科建设，促进种业产业持续健康发展。 相似文献
16.
Summary Between 1993 and 1998 205 different potato cultivars and 1220 accessions/genotypes of wild and cultivated potato species from
the IPK Genebank Gatersleben were evaluated. Parameters interesting for starch isolation and especially for the use of starch
were determined. Altogether, there was a higher variability in wild potato species than in cultivated potatoes for all characteristics
investigated: dry matter content, starch content, protein content, amylose content and mean particle diameter of starch granules. 相似文献
17.
In field experiments conducted over 2 years in Mediterranean conditions, five winter and five spring triticales were evaluated for forage and grain production in the same cropping season. The experiments had two treatments, namely harvesting for grain only, and dual-purpose forage and grain production. In the latter treatment, forage was cut when the first node was detectable (Zadoks' stage 31), without removing the apical meristems. Grain was harvested when ripe (Zadoks' stage 92) in both cut and uncut plots.
Environmental conditions affected grain production and protein content more than forage yield and quality. Winter triticales yielded about 43% more forage than spring types, but after forage removal the spring types yielded about 36% more grain than winter triticales.
Reductions in grain yield after clipping were more pronounced in winter (32%) than in spring (19%) types. Forage crude protein content was significantly higher in the spring types studied (24.6%) than in the winter types (23.5%), the opposite being true for fibre content (20.7 and 21.6% respectively). Grain crude protein content did not differ between grain and dual-purpose treatments, but was higher in the spring triticales (12.8%) than in the winter types (11.9%). There was more variability for the measured traits within the winter triticales studied than within the spring types. 相似文献
Environmental conditions affected grain production and protein content more than forage yield and quality. Winter triticales yielded about 43% more forage than spring types, but after forage removal the spring types yielded about 36% more grain than winter triticales.
Reductions in grain yield after clipping were more pronounced in winter (32%) than in spring (19%) types. Forage crude protein content was significantly higher in the spring types studied (24.6%) than in the winter types (23.5%), the opposite being true for fibre content (20.7 and 21.6% respectively). Grain crude protein content did not differ between grain and dual-purpose treatments, but was higher in the spring triticales (12.8%) than in the winter types (11.9%). There was more variability for the measured traits within the winter triticales studied than within the spring types. 相似文献
18.
种子加工及检验是种子向市场流通的关键,是商业化育种中赋予种子商品属性不可或缺的环节。种子加工、检验理论与技术的研究是完善种业产业链、实现规模化商业育种、夯实"育繁推一体化"种业科学的重要一环。中国的种业科学技术体系正在形成与完善之中,受制于行业发展水平,种子加工及检验理论与技术相对薄弱。本文回顾了种子加工及检验理论与技术研究发展历程,提出了完善中国种业科学发展的策略与建议。围绕种业发展的需要,在做好品种优质化繁育的基础上,分析种子加工及检验理论与技术的新方向和新需求,加强种业应用性研究,建立先进的种子质量检验体系,研制适合现代种业发展需求的种子加工设备,打造种子加工产业标准化生产体系,完善种业科学技术学科建设,促进种业产业持续健康发展。 相似文献
19.
ZHU Mingdong WEI Xiangjin XIE Hongjun TANG Guohua ZENG Xiaoshan WANG Jianlong HU Peisong YU Yinghong 《中国水稻科学》2019,33(5):401-406
Seed processing and testing hold the key to the market circulation of seeds, and are an indispensable link in commercial breeding to endow seeds with commodity attributes. The research of seed processing and testing theory and technology is an important link in improving the seed industry chain, realizing large-scale commercial breeding and consolidating the seed industry science of ‘integration of breeding, propagation and promotion’. The scientific and technological system of seed industry in China is being formed and perfected, which is subject to the development level of the industry. The theory and technology of seed processing and testing are relatively weak. We reviewed the development of seed processing and testing theory and technology, and put forward strategies and suggestions to improve the sound development of China’s seed industry. In order to meet the needs of seed industry development and on the basis of high quality breeding of varieties, the new directions and demands of seed processing and testing theory and technology were analyzed. We will work to strengthen the applied research of seed industry, establish advanced seed quality inspection system, improve seed processing equipment suitable for the development needs of modern seed industry, establish standardized production system of seed processing industry, develop the scientific and technological disciplines of seed industry, and promote the sustainable and healthy development of seed industry. 相似文献