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研究鸭呼肠孤病毒(Duck reovirus,DRV)σC蛋白与宿主因子之间的相互作用,对揭示σC蛋白在病毒复制过程中的功能及该病毒的致病机理具有重要作用。本研究采用免疫共沉淀法及质谱分析技术,筛选出与σC蛋白可能发生互作的宿主相关蛋白,并对所筛选出的宿主细胞蛋白进行了GO注释和KEGG代谢通路注释。结果表明,与空白对照组比较,获得与σC蛋白相互作用的宿主细胞蛋白有100个;筛选出的这些宿主细胞蛋白中,在病毒感染后不同时间点差异表达量均上升3倍以上的蛋白有6个,分别与病原致病性、蛋白质代谢途径、细胞信号通路转导有关。该研究结果为进一步探讨σC蛋白的生物学功能奠定了基础,也为揭示σC蛋白与宿主因子相互作用进而调控DRV复制等机制研究提供线索。 相似文献
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RIG-I样受体（RLRs）是细胞质中重要的模式识别受体（PRRs）,LGP2是其重要的家族成员之一。LGP2在抗病毒天然免疫应答中具有特殊功能,能够双向调控RIG-I、MDA5介导的I型干扰素（IFNs）信号通路。在不同病毒感染宿主的过程中,LGP2也表现出明显的功能差异。在双链DNA病毒中,LGP2在RIG-I介导的信号传导过程中起正向调节作用;在单链DNA病毒中,LGP2能够促进CARDs区失活的RIG-I与非典型泛素链结合,从而诱导I型IFNs产生;在双链RNA病毒中,正常表达可增强RIG-I、MDA5对dsRNA的识别能力;在正义单链RNA病毒中,LGP2参与对正义单链RNA病毒的识别过程;在负义单链RNA病毒中,LGP2有时可作为IFNs的诱导剂。另外,LGP2在适应性免疫应答中也发挥着重要作用,能够通过不同途径调控T细胞存活与凋亡。目前在调控RIG-I、MDA5介导的信号通路与抗病毒免疫应答中,尚不清楚LGP2发挥的确切功能和其作用机制,未来可进一步加深LGP2在细胞免疫应答中的作用及机制研究。本文就近年来LGP2在RLRs介导的信号通路和抗病毒免疫应答中发挥的作用作综述,以期为LGP2的进一步研究和机体抵御病毒感染新机制提供参考。 相似文献
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长链非编码RNA （long noncoding RNA,lncRNA）是一种核酸长度>200 nt、不编码或具有有限编码能力的RNA,早期一直被认为是遗传暗物质,但伴随分子生物学技术的进步,越来越多的lncRNAs被发掘。研究表明,lncRNA作为基因表达的关键调控因子,能在组蛋白修饰、转录调控和转录后调控等方面影响基因表达,几乎参与所有的细胞生物学过程。顶复门原虫是一类专性胞内寄生虫,入侵机制复杂,对顶复门原虫与宿主的互作研究已成为新型抗虫药物的研发基础。近年来研究发现,lncRNA作为一类新型调控因子广泛参与到顶复门原虫与宿主细胞相互作用中,包括感染免疫、发育分化和信号传导等细胞生物学过程,既可帮助宿主抵御寄生虫入侵,也可协助寄生虫在胞内增殖发育,对疾病的发生发展具有重要的调控作用。笔者通过对lncRNA的生物学分类及其在细胞生理过程中的主要功能进行概述,综合近年来lncRNA在以弓形虫、疟原虫和隐孢子虫为主要代表的顶复门原虫的相关研究,系统梳理了lncRNA在宿主免疫反应、寄生虫发育分化和表观遗传调控等方面的作用机制,以期为顶复门原虫致病机理研究及高效防控技术研发提供新的思路和参考。 相似文献
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为了探索引起H5N1高致病性禽流感病毒(highly pathogenic avian influenza virus,HPAIV)致病性差异的宿主因子,选取了一对主要由PA基因造成在小鼠上致病性差异的H5N1禽流感病毒(CK10与GS10),通过病毒感染A549细胞,利用PA抗体进行免疫沉淀(immunoprecipitation,IP)试验以筛选与PA互作的宿主蛋白,并进行LC-MS/MS鉴定。结果发现与低致病性的GS10相比,共有160种宿主蛋白能够特异性与高致病性CK10病毒PA蛋白结合。对这160种蛋白进行生物信息学分析,通过Gene Ontology(GO)注释分析发现这些蛋白主要参与翻译、基因表达、病毒转录和病毒感染等生物学进程,通过KEGG通路分析发现这些蛋白主要参与的细胞通路包括翻译、传染病与信号转导等。此后,利用免疫共沉淀技术(co-immunoprecipitation,Co-IP),证实所筛选的宿主蛋白eEF1A1能够特异性地与CK10病毒PA蛋白相互作用。本研究筛选到了致病性不同的禽流感病毒的差异互作宿主蛋白,可为进一步了解流感病毒复杂的致病机制提供参考。 相似文献
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长链非编码RNA(lncRNA)是指长度大于200个核苷酸,不表现出任何蛋白质编码潜能的一类RNA。lncRNA参与调控机体的生长发育、细胞凋亡、增殖和分化等,与肿瘤、心血管疾病和感染性疾病等密切相关,为lncRNA的研究能够为复杂疾病的预测、诊断和治疗提供新的依据。论文主要介绍lncRNA与疾病的相关研究进展。 相似文献
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D. M. HAIG 《Research in veterinary science》2001,70(3):205-219
During the co-evolution of viruses with their vertebrate hosts, the DNA viruses have acquired an impressive array of immunomodulatory genes to combat host immune responses and their hosts have developed a sophisticated immune system to contain virus infections. In order to replicate, the viruses have evolved mechanisms to inhibit key host anti-virus responses that include apoptosis, interferon production, chemokine production, inflammatory cytokine production, and the activity of cytotoxic T-cells, natural killer cells and antibody. In addition, some of the viruses encode cytokine or chemokine homologues that recruit or expand cell numbers for infection or that subvert the host cellular response from a protective response to a benign one. The specificity of the viral immunomodulatory molecules reflects the life cycle and the pathogenesis of the viruses. Herpesviruses achieve latency in host cells by inducing cell survival and protecting infected cells from immune recognition. This involves interference with cell signal transduction pathways. Many of the viral immunomodulatory proteins are homologues of host proteins that appear to have been pirated from the host and reassorted in the virus genomes. Some of these have unique functions and indicate novel or important aspects of both viral pathogenesis and host immunity to viruses. The specific example of orf virus infection of sheep is described. 相似文献
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THE CHARACTERISATION AND PATHOGENICITY OF PORCINE ENTEROVIRUSES ISOLATED IN VICTORIA 总被引：1，自引：0，他引：1
SUMMARY Approximately 23 viruses were isolated from healthy pigs, pigs with encephalitis, and in cases of reproductive failure. Five viruses were identified as enteroviruses and a total of 10 isolates were shown to cross-react serologically to varying degrees. Twenty viruses were neutralised by a reference antiserum of serotype 8 porcine enterovirus. Intracerebral inoculation of colostrum-deprived piglets with 2 of the characterised viruses caused lesions of encephalomyelitis which were not induced by oral infection. Intrafoetal inoculation of 2 sows with one characterised faecal isolate caused foetal death and abortion, but no adverse effects followed oral dosage. 相似文献
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多重PCR方法用于检测猪圆环病毒2型、猪细小病毒、猪伪狂犬病病毒、猪繁殖与呼吸综合征病毒诊断方法的建立 总被引：2，自引：0，他引：2
本文建立了一种能够同时检测猪圆环病毒2型（PCV2）、猪细小病毒（PPV）、猪伪狂犬病病毒（PRV）、猪繁殖与呼吸综合征病毒（PRRSV）的多重PCR方法（mPCR）。借助于Oligo6.0引物设计软件,设计了4对引物分别用于扩增PCV2ORF2基因353bp片段、PPVNS-1基因271bp片段、PRRSVMN基因美洲型434bp片段、PRVgB基因194bp片段。通过人为混合以上4种病毒进行特异性及敏感性试验,结果表明,该方法具有很好的特异性和敏感性。对猪瘟病毒、大肠杆菌和双蒸水的PCR扩增结果均为阴性;该mPCR方法对PPV的最低检测量为8.64×10-3μg,PRV的最低检测量为2.36×10-3μg,PRRSV的最低检测量为3.68×10-3μg,PCV2的最低检测量为2.90×10-4μg。该方法的建立对临床上PCV2、PPV、PRV、PRRSV的鉴别诊断以及以上这4种病毒的流行病学调查具有十分重要的意义。 相似文献
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Isolation and genetic characterization of avian origin H9N2 influenza viruses from pigs in China 总被引：7，自引：1，他引：6
Yu H Hua RH Wei TC Zhou YJ Tian ZJ Li GX Liu TQ Tong GZ 《Veterinary microbiology》2008,131(1-2):82-92
As pigs are susceptible to infection with both avian and human influenza A viruses, they have been proposed to be an intermediate host for the adaptation of avian influenza viruses to humans. In April 2006, a disease caused by highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) occurred in several pig farms and subsequently overwhelmed almost half of China with more than 2,000,000 cases of pig infection. Here we report a case in which four swine H9N2 influenza viruses were isolated from pigs infected by highly pathogenic PRRSVs in Guangxi province in China. All the eight gene segments of the four swine H9N2 viruses are highly homologous to A/Pigeon/Nanchang/2-0461/00 (H9N2) or A/Wild Duck/Nanchang/2-0480/00 (H9N2). Phylogenetic analyses of eight genes show that the swine H9N2 influenza viruses are of avian origin and may be the descendants of A/Duck/Hong Kong/Y280/97-like viruses. Molecular analysis of the HA gene indicates that our H9N2 isolates might have high-affinity binding to the alpha2,6-NeuAcGal receptor found in human cells. In conclusion, our finding provides further evidence about the interspecies transmission of avian influenza viruses to pigs and emphasizes the importance of reinforcing swine influenza virus (SIV) surveillance, especially after the emergence of highly pathogenic PRRSVs in pigs in China. 相似文献
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Chlamydophila abortus targets the placenta, causing tissue damage, inflammation and abortion (enzootic abortion of ewes). It is one of the main infectious causes of abortion in ewes, resulting in major economic losses to agricultural industries worldwide. Although ruminants and pigs are the principal hosts, humans are also susceptible to infection. Control of disease requires a host inflammatory response, which is likely to contribute to pathology and abortion. Mouse models have been widely used to provide insight into the role of specific immune cells in controlling infection and disease. The use of such model systems for investigating the mechanisms of abortion, latency, persistence, and immunity to reinfection will result in the identification of novel vaccine control strategies for sheep. 相似文献
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Sang Y Rowland RR Blecha F 《Animal health research reviews / Conference of Research Workers in Animal Diseases》2011,12(2):149-167
Innate immunity provides frontline antiviral protection and bridges adaptive immunity against virus infections. However, viruses can evade innate immune surveillance potentially causing chronic infections that may lead to pandemic diseases. Porcine reproductive and respiratory syndrome virus (PRRSV) is an example of an animal virus that has developed diverse mechanisms to evade porcine antiviral immune responses. Two decades after its discovery, PRRSV is still one of the most globally devastating viruses threatening the swine industry. In this review, we discuss the molecular and cellular composition of the mammalian innate antiviral immune system with emphasis on the porcine system. In particular, we focus on the interaction between PRRSV and porcine innate immunity at cellular and molecular levels. Strategies for targeting innate immune components and other host metabolic factors to induce ideal anti-PRRSV protection are also discussed. 相似文献
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Myxoma virus (MV) is a poxvirus that evolved in Sylvilagus lagomorphs, and is the causative agent of myxomatosis in European rabbits (Oryctolagus cuniculus). This virus is not a natural pathogen of O. cuniculus, yet is able to subvert the host rabbit immune system defenses and cause a highly lethal systemic infection. The interaction of MV proteins and the rabbit immune system has been an ideal model to help elucidate host/poxvirus interactions, and has led to a greater understanding of how other poxvirus pathogens are able to cause disease in their respective hosts. This review will examine how MV causes myxomatosis, by examining a selection of the identified immunomodulatory proteins that this virus expresses to subvert the immune and inflammatory pathways of infected rabbit hosts. 相似文献
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A randomised, double-blinded, placebo-controlled study into the effectiveness and safety of a vaccine against porcine reproductive and respiratory syndrome virus (PRRSV) was carried out on three farms with a history compatible with chronic PRRSV infection; representative groups of sows and gilts were injected with a live vaccine against PRRSV, and during the next six weeks no side effects were observed. The remaining sows and gilts on the three farms were then vaccinated with the same vaccine. Again, no side effects were observed. There were significant reductions in abortion, reproductive disease, returns to oestrus and the numbers of stillborn pigs per sow, and significant increases in the numbers of liveborn pigs per sow and weaned pigs per sow among the vaccinated animals. 相似文献