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1.
鮸鱼亲鱼培育及其人工繁殖的研究   总被引:13,自引:0,他引:13  
采用自然苗培育成的1龄鱼,通过“春肥、夏育、秋繁、冬保”8字方针培育成亲鱼,性成熟率达到95%。经LHRH-A30.8~1.8μg/kg催产并放人产卵池自行产卵,获产率达到96.83%。获受精卵650万余粒,受精率达到52%。受精后21.8 h孵化出鱼苗,孵化率达到70%。  相似文献   

2.
鳡鱼人工繁殖技术研究   总被引:4,自引:0,他引:4  
试验亲本来源于长江武汉金口江段及附属湖泊斧头湖、鲁湖的野生鳡鱼,经6个月暂养或两年池塘驯养后,于2005年~2006年6月分4批共11组进行人工繁殖试验,产卵总量1020万粒,受精卵816万粒,孵出水花365万尾。2年试验平均每组次产卵92.7万粒,受精74.2万粒,孵出仔鱼33.1万尾。初步探明了鳡鱼人工繁殖的方法及规律,其催产率、受精率、孵化率分别为80%~100%、30.4%~88.5%、86.3%~88.8%,同时还对影响人工繁殖的相关因素进行了初步探讨。  相似文献   

3.
野生黄颡鱼雌鱼产卵前后肌肉脂肪酸的组成   总被引:1,自引:1,他引:0  
采用毛细管气相色谱法,对贵州锦江河繁殖期产卵前后黄颡鱼肌肉中的脂肪酸组成进行了测定。结果显示:黄颡鱼产卵前后肌肉中脂肪酸种类都比较丰富,含有有22种脂肪酸。油酸(C18:1n-9)所占比例最高,其次是软脂酸(C16:0)、硬脂酸(C18:0)和棕榈酸(C16:In-7),分别占总脂肪酸的40.31%-43.90%、22.94%-25.05%、6.05%-7.75%、6.26%-7.00%。廿碳五烯酸(C20:5n-3,EPA)和廿二碳六烯酸(C22:6n-3,DHA)之和占4.69%~5.38%,其中EPA约占总脂肪酸的1.4%,DHA占3.6%。同产卵前相比,软脂酸(C16:0)、硬脂酸(C18:0)、亚油酸(C18:2n-6)和花生四烯酸(C20:4n-6,ARA)所占比例下降较大,而C15:0、C22:0、亚麻酸(C18:3n-3)的比例显著升高。研究表明在黄颡鱼产卵后的饲料配制中,需要适当添加脂类物质,以恢复其在产卵后的体质。  相似文献   

4.
奥尼鱼是奥利亚罗非鱼(雄)和尼罗罗非鱼(雌)的杂交一代。由于它具有生长快、易养高产、肉质好的优点,日益受到养殖者和消费者的喜爱。现将奥尼鱼的鱼苗培育及高产养殖技术总结如下。1奥尼色的生物学特性1.1雄性率高奥尼龟雄性率在92%以上。由于雌鱼少,且当年不产卵,次年产卵也少,所以没有因大量繁殖而造成密度过大的情况。1.2生长快、产量高奥尼鱼具有明显的杂交优势。生长快,刚孵出的鱼苗养至0.5公开的上市规格只需5个月;一年可养两次,亩放800至1500尾,年亩产奥尼鱼800~1500公斤。1.3食性杂、易养、易捕1.4抗病、耐缺氧…  相似文献   

5.
1.产卵时间黄颡鱼在自然条件下,一般于5月底开始产卵活动,一直持续到7月下旬,这一点通过对其性腺发育阶段解剖观察,和在湖泊中捕获最早的一批天然苗一般在7月中旬即可证明,然而若通过人工精养进行产前强化培育,一般可使产卵时间提前10~15天左右。2.亲本培育年底起捕时从池塘或湖泊中收集亲本鱼,要求雌鱼50克以上,雄鱼100克以上,放入池塘中进行专池培育,只套养少量花白鲢以控制水质。通过以各种冰鲜杂鱼虾、螺蚌肉等动物性肉糜与饲料厂预混料,混合制成含动物蛋白40%左右的混合饲料投喂,日投喂1~2次,…  相似文献   

6.
《淡水渔业》1973,(5):11-13
家鱼人工繁殖产卵方式有两种:人工授精和自行产卵,我场则以人工授精为主,几年来,我场广大工人和技术人员以《矛盾论》、《实践论》为武器,狠批刘少奇一类骗子散布的唯心论的先验论,初步掌握了家鱼人工授精方面的基本规律,积累了一些经验。亲鱼产卵率每年都达到95%以上,亲鱼个体取卵数高(每公斤体重的雌鱼产卵达10万左右),经过多年实践,我场有如下几点体会。  相似文献   

7.
黑龙江茴鱼的人工繁殖技术研究初报   总被引:2,自引:0,他引:2  
黑龙江茴鱼平均产卵量为2 256粒/尾,属分批产卵型。在平均水温7.7℃(4.2~10.8℃)情况下:100~110℃·d时眼点明显;120~140℃·d开始破膜;200~210℃·d破膜结束;250℃·d左右开始上浮;270℃·d左右开口。发眼率为54.86~71.07%;孵化率为72.15~77.63%;上浮率为51.53~66.56%。  相似文献   

8.
缺帘鱼全人工繁殖技术研究   总被引:1,自引:0,他引:1  
徐绍刚  田照辉  朱华  李建平 《水产科技情报》2008,35(5):224-225, 228
利用地下井水,通过人工控温,将引进的缺帘鱼鱼种培育成亲鱼,再对亲鱼进行强化培育,待其达性成熟后进行人工催产.较详细地研究了缺帘鱼亲鱼强化培育的条件,亲鱼的雌雄鉴别技术,催产剂的组合和使用剂量.根据试验结果认为,缺帘鱼亲鱼在水泥池内培育能达到性成熟,人为控制环境条件能控制其产卵时间.  相似文献   

9.
利用人工催产、自然产卵受精技术,对30组蓝鳃太阳鱼(♂)和绿鳃太阳鱼(♀)亲鱼成功地进行人工杂交繁殖试验。在第2次注射催产剂36h后亲鱼开始产卵,4d后产卵结束,共产卵9组,产卵率30%,产卵4.95万粒,受精卵约4万粒,受精率约82.2%。经38~50 h孵化,出苗3.4万尾,孵化率85%。获3 cm苗种2.5万尾,成活率73.5%  相似文献   

10.
池养瓦氏黄颡鱼全人工繁殖的初步研究   总被引:4,自引:0,他引:4  
2002年5月12日-6月5日,以自繁自育的2龄瓦氏黄颡鱼作亲本,开展大批量全人工繁殖,共催产亲鱼163.8kg,其中雌亲鱼87.5kg,679尾,雄亲鱼76.3kg,323尾。人工产卵率99%,自行产卵率为88%,共产卵约156万粒,平均受精率为54%,孵化率为44%,孵出鱼苗37.8万尾。  相似文献   

11.
The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.  相似文献   

12.
13.
In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.  相似文献   

14.
Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China.  相似文献   

15.
16.
The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus.  相似文献   

17.
Growth hormone (GH) and reproduction: a review   总被引:5,自引:0,他引:5  
Interaction between growth and reproduction occurs in many vertebrates and is particularly obvious at certain stages of the life cycle in fish. Endocrine interactions between the gonadotropic axis and the somatotropic axis are described, the potential role of GH being emphasised. A comparative analysis of these phenomena in mammals, amphibians and fish, suggests a specific role of GH in the physiology of puberty, gametogenesis and fertility. It also shows the original contribution made by studies on the fish model in this field of investigations.  相似文献   

18.
Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL?1 (1.25 ng well?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation.  相似文献   

19.
20.
A 5 × 3 factorial growth trial was conducted to evaluate optimal dietary protein and lipid levels (dietary protein level, DP; dietary lipid level, DL) for juvenile Sillago sihama (S. sihama) (2.0 ± 0.02 g, initial weight). Fish were fed 15 diets containing 5 DPs (350, 400, 450, 500 and 550 g/kg) and 3 DLs (60, 90 and 120 g/kg) for 8 weeks. The interaction between proteins and lipids significantly influenced the feed conversion ratio, condition factor, body composition, antioxidant indices and lipase activity (p < .05). DP 450 g/kg showed the highest average final body weight. DPs 500 and 550 g/kg significantly decreased the protein efficiency ratio (p < .05). DL 120 g/kg showed the highest percentage weight gain. The low feed conversion ratio was found in diets P45L12, P55L9 and P55L12. Diet P45L12 showed high superoxide dismutase activities. DP 450 g/kg showed the lowest average malondialdehyde content. Lipase activity was increased by increasing DP (p < .05) with a fall at DP 550 g/kg. Under the present experimental conditions, the optimal DP for S. sihama was 450 g/kg under the DL 120 g/kg.  相似文献   

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