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1.
为了解大雁、斑头雁及天鹅源沙门氏菌流行特征和脉冲场凝胶电泳（PFGE）分型,本研究对33株沙门氏菌进行血清型鉴定,采用PFGE对其进行基因分型,并用BioNumerics 6.6软件进行聚类分析。鉴定出丙型副伤寒沙门氏菌6株、汤卜逊沙门氏菌19株、乙型副伤寒沙门氏菌1株、伊鲁木沙门氏菌1株、奥斯陆沙门氏菌1株、5株未定型。PFGE聚类分型共分为16个型别,其相似度为53.7%~100.0%,且大多数菌株与人源菌株相似度超过80%。结果表明,大雁、斑头雁及天鹅源沙门氏菌相同血清型PFGE带型高度相似,可能来自同一克隆株,不同血清型PFGE带型差别较大。 相似文献
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鸡沙门氏菌脉冲场凝胶电泳分型研究 总被引：1，自引：0，他引：1
为应用脉冲场凝胶电泳(PFGE)从分子水平上对禽源沙门氏菌之间的差别进行分析和研究,本研究采用自动微生物鉴定仪对12株疑似鼠伤寒沙门氏菌和15株疑似鸡白痢沙门氏菌进行了鉴定.以限制性核酸内切酶Xba I对其全基因组DNA进行酶切、PFGE分型,Info Quest FP聚类分析软件对电泳结果进行分析.结果显示,共鉴定出11株鼠伤寒沙门氏菌、1株圣保罗沙门氏菌、6株阿姆斯特丹沙门氏菌、5株亚拉巴马沙门氏菌、1株鸡白痢沙门氏菌、2株纽波特沙门氏菌和2株肠炎沙门氏菌,一共分为12个PFGE型.实验结果表明,江苏地区存在有不常见的沙门氏菌血清型,同种血清型之间的基因型差别较小(相似值为0.85～1),不同血清型之间差别较大(相似值为0.45～0.70).PFGE能从分子水平上对禽源沙门氏菌的基因组DNA进行分型. 相似文献
3.
J Sander C R Hudson L Dufour-Zavala W D Waltman C Lobsinger S G Thayer R Otalora J J Maurer 《Avian diseases》2001,45(4):1044-1049
Control of carcass contamination requires knowledge of the source and dynamics of spread of Salmonella in commercial poultry production. We examined Salmonella contamination at a U.S. commercial quail operation. Pulsed-field gel electrophoresis (PFGE) was used to type isolates in order to trace Salmonella throughout this production environment. During a 6-mo survey, Salmonella serotypes hadar, typhimurium, typhimurium variant Copenhagen, and paratyphi were encountered within this poultry operation. Ninety-four percent of the Salmonella isolated from breeder and production houses and from carcass rinses belonged to Salmonella serotypes typhimurium variant Copenhagen and hadar. There were six distinct S. typhimurium variant Copenhagen genetic types, as identified by PFGE, present within this particular poultry operation. Seventy-nine percent of S. typhimurium variant Copenhagen identified from the environment of the breeder and production houses produced the same PFGE pattern. Thirty-eight percent of S. typhimurium Copenhagen isolated from carcass rinses and the breeder house shared the same PFGE DNA pattern. This study demonstrates the transmission of salmonellae throughout this production environment, from the breeders to their progeny and to the birds ultimately processed for human consumption. 相似文献
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食品动物源沙门氏菌质粒介导喹诺酮类耐药基因的检测与分析 总被引：2，自引：0，他引：2
为了解食品动物源沙门氏菌质粒介导喹诺酮类耐药性(Plasmid-mediated quinolone resistance,PMQR),采用微量肉汤稀释法和PCR方法,检测了316株食品动物源沙门氏菌对20种抗菌药物的敏感性,以及菌株中PMQR基因的携带率.结果显示:316株沙门氏菌对20种抗菌药物呈不同程度的耐药性,95.57％菌株为多重耐药菌;316株菌中未检出qnrA、qnrC、qnrD、qnrS和qepA基因,7.91％菌株检出qnrB基因,15.19％菌株检出aac(6 ′ )-Ib-cr基因,7.91％菌株检出oqxA基因,8.86％菌株检出oqxB基因,这是首次在沙门氏菌中发现oqxAB基因;98.11％PMQR阳性菌同时携带2种及以上的耐药基因,呈8～17耐的多重耐药性,其中以qnrB和aac(6′)-Ibcr基因型为主;53株PMQR阳性菌分属于5种不同的基因型,耐药表型或耐药基因型不同的菌株却有相同的PFGE谱型.本次检测的316株食品动物源沙门氏菌耐药较为严重;菌株主要携带qnrB、aac(6 ′ )-Ib-cr及oqxAB基因;不同来源菌株存在同一耐药克隆株的流行. 相似文献
5.
SHEN Xue-huai ZHANG Dan-jun PAN Xiao-cheng ZHAO Rui-hong DAI Yin HU Xiao-miao 《中国畜牧兽医》2017,44(12):3663-3669
In order to investigate the situation of predominant strain and antibiotic resistance of avian Salmonella in Anhui province, 42 strains Salmonella were isolated and identified from 116 suspected samples by culture characteristics, microscopic examination, biochemical test, PCR and serological identification,then drug resistance was analyzed by Kindy-Bauer method. There were 5 groups of 11 serotypes in 42 strains Salmonella, of which 30 strains belonged to B serogroup, 2 strains to A serogroup, 7 strains to D serogroup, 2 strains to C1 serogroup, 1 strain to C2 serogroup, and Salmonella Typhimurium of B serogroup were the predominant serotypes. All Salmonella isolates resistant rates to amikacin, levofloxacin and ciprofloxacin were less than 20%, and the resistance rates to ampicillin, streptomycin, furazolidone, spectinomycin, cotrimoxazole and doxycycline were more than 50%, while resistance rate to ampicillin was 97.62%, and 41 isolates had multiple drug resistance. The results showed that Salmonella Typhimurium was the predominant serotypes of avian Salmonella in Anhui, and isolates showed multiple drug resistance. This study would provide the data basis for the comprehensive prevention and treatment of clinical medicine of avian Salmonella. 相似文献
6.
为了解安徽地区禽源沙门氏菌的流行菌株和耐药情况,本研究通过培养特性、镜检、生化试验、PCR和血清型鉴定等,对疑似发病禽的116份病料进行沙门氏菌分离鉴定,并采用Kindy-Bauer法对分离菌株进行耐药性分析。试验共分离到42株禽源沙门氏菌,其中包含5个大群11种血清型。B群30株、A群2株、D群7株、C1群2株、C2群1株,其中以B群鼠伤寒沙门氏菌为优势菌株。42株分离菌对阿米卡星、左氧氟沙星和环丙沙星的耐药率均低于20%,而对氨苄西林、链霉素、呋喃唑酮、大观霉素、复方新诺明和多西环素的耐药率达50%以上,其中对氨苄西林的耐药率达97.62%,且41株分离菌株存在多重耐药。结果表明,目前安徽地区禽源沙门氏菌优势流行株是鼠伤寒沙门氏菌,流行菌株呈现较严重的耐药性。本研究为指导临床用药及禽源沙门氏菌的综合防制提供数据支撑。 相似文献
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8.
本试验从华南地区发病肉鸽肠道和肝脏中用BS培养基分离、纯化沙门氏菌,并进行多项生化试验和血清型鉴定试验,结果显示各项生化检测指标均符合沙门氏菌的特点;血清型鉴定试验结果表明该分离菌属沙门氏菌属A-F群。进一步对该分离菌的16S rRNA片段测序及遗传进化分析,确定该分离菌为沙门氏菌菌株,与鼠伤寒沙门氏菌同源性为100%。对该分离菌进行动物回归试验,结果表明该沙门氏菌菌株为华南地区肉鸽的致病菌株。该结果为进一步研究华南地区肉鸽沙门氏菌的致病机理和防控奠定了基础。 相似文献
9.
ZHANG Yujie SUN Ning LIU Dong YU Jing LIU Hongxiang YU Kechao REN Yanbei GAO Tianzuo YANG Shaoyan GONG Xiao ZOU Min ZHANG Yulong WANG Yuchao ZHONG Sheng MA Dong DU Yuanzhao 《畜牧兽医学报》1956,51(11):2765-2777
From October 2017 to May 2019, 67 samples of typical goslings gout were collected from Guangdong, Sichuan, Hebei, Shandong, Anhui, Liaoning provinces and other vast goose-raising areas in China. The results showed that goose astrovirus was detected in all samples and about 94.03% of the samples were infected by two different kinds of goose astrovirus. The pathogenicity of goose astrovirus FLX variant strain (named SCCD) which could stably proliferate in goose embryos and kill 10-day-old goose embryos was more virulent than goose astrovirus FLX strain. The new type of goose astrovirus strain (named SDPD) could't stably proliferate in goose embryos and SPF chicken embryos. The whole genome nucleotides homology of goose astrovirus FLX strain with goose astrovirus SCCD strain and the new type goose astrovirus SDPD strain were 89.7% and 58.1%, the amino acids homology of ORF1b gene were 98.4% and 61.0%, the amino acids homology of ORF2 gene were 81.0% and 42.5%, respectively. The newly isolated goose astrovirus strains were inoculated into 1-day-old healthy goslings, the results showed that although the goose astrovirus SCCD strain and the new type goose astrovirus SDPD strain could cause the death of goslings and the changes of hepatitis, kidney swelling and weight loss, none of the goslings showed typical gout characteristics (urate deposition in the organs of the body), however, 44% of goslings were characterized with typical gout which was consistent with the symptoms of natural disease after coinfected by the two goose astrovirus strains. Therefore, the pathogenic agents of goslings gout may be two different kinds of goose astrovirus, namely the new type of goose astrovirus and the variant of goose astrovirus FLX. 相似文献
10.
鹅星状病毒的分离鉴定及全基因组序列分析 总被引：1，自引：1，他引：0
2017年10月—2019年5月,本实验室从广东、四川、河北、山东、安徽、辽宁等广大养鹅地区采集了67份典型雏鹅痛风样品进行了实验室检测以及病原的分离鉴定,检测结果表明：所有样品中均检测到了鹅星状病毒,并且约有94.03%的样品为两个不同种的鹅星状病毒混合感染。病原分离结果表明：鹅星状病毒FLX株的变异株病毒（命名为SCCD）可以在鹅胚上稳定增殖,并且能稳定致死10日龄鹅胚,致病力较鹅星状病毒FLX增强;新型鹅星状病毒株（命名为SDPD）不能在鹅胚和SPF鸡胚上稳定增殖。病毒的基因组测序结果表明：鹅星状病毒FLX株与鹅星状病毒FLX的变异株病毒SCCD株、新型鹅星状病毒SDPD株全基因组核苷酸相似性分别为89.7%、58.1%,ORF1b基因氨基酸相似性分别为98.4%、61.0%,ORF2基因氨基酸相似性分别为81.0%、42.5%。将新分离的鹅星状病毒株接种1日龄健康雏鹅,结果表明,鹅星状病毒SCCD株和鹅星状病毒SDPD株虽然能使雏鹅发生死亡,引起肝炎、肾肿胀和增重减少等变化,但雏鹅均无典型痛风（脏器尿酸盐沉积）表现,而两种鹅星状病毒株混合攻毒能使44%的雏鹅发生典型痛风,并与临床发病症状一致。因此,临床上引起雏鹅痛风的病原可能是两种鹅星状病毒,即新型鹅星状病毒和鹅星状病毒FLX的变异株病毒。 相似文献
11.
【目的】为研究全基因组测序技术在猪源沙门氏菌血清型和耐药性检测方面的应用能力。【方法】以60株猪源沙门氏菌为研究对象,应用传统玻片凝集法和微量肉汤稀释法对其进行血清分型和耐药性检测,并提取所有菌株核酸进行全基因组测序组装,利用SeqSero2和ResFinder4在线预测每株菌的血清型和耐药基因,然后与传统方法进行比较分析。【结果】血清凝集试验共鉴定出8种沙门氏菌血清型,而全基因组测序鉴定出9种血清型,二者分型结果的符合率为90.0%;对11种抗菌药物的药敏试验显示：沙门氏菌对四环素（73.3%）的耐药率最高,其次为氨苄西林（66.7%）、磺胺异噁唑（66.7%）和复方新诺明（53.3%）,基于全基因组预测的恩诺沙星、美罗培南、头孢噻呋和阿奇霉素的耐药性与药敏试验结果完全一致,对氨苄西林、磺胺异噁唑、四环素预测的结果符合率也均大于90.0%。【结论】全基因组测序分析的沙门氏菌血清型和耐药性与常规检测方法的符合率较高,且具有操作简便的优势,可以作为分析沙门氏菌血清型和耐药性的有效方法。 相似文献
12.
屠宰猪肠道沙门氏菌的分离鉴定、耐药性分析及致病性试验 总被引：2，自引：0，他引：2
为了解广西南宁市猪源沙门氏菌的污染状况、耐药状况及致病力情况,在南宁市某生猪屠宰场随机直接从131头屠宰猪的肠道采集样品,采用鉴别培养基分离,生化鉴定的方法对样品中的沙门氏菌进行分离鉴定,并采用标准K-B纸片法对分离菌株进行25种抗生素敏感试验,最后对分离株进行小白鼠致病性试验。结果从131份屠宰猪的肠道中共分离到沙门氏菌45株,检出率为34.35%;其中鼠伤寒沙门氏菌14株,甲型副伤寒杆菌2株,肠炎沙门氏菌3株。45株分离菌株全部耐药,耐药率高达100%,其中44株为多重耐药菌株,占97.78%。45株沙门氏菌中有40株对小白鼠具有致病性,致病率达88.89%。这表明南宁市的屠宰猪存在一定程度的沙门氏菌污染,并且分离菌株存在较严重的耐药现象以及具有较强的致病性。应采取有效措施控制沙门氏菌在猪群中的污染和限制抗生素在养猪过程中的使用并严格遵守休药期,以减少细菌耐药性的产生,保障猪肉及猪肉制品的食品安全。 相似文献
13.
LI Chen ZHANG Shuang-xiang ZHOU Bi-jun CHENG Zhen-tao WEN Ming MA Guang-qiang WANG Wei HU Xing-yi ZHANG Hai DING Zun-e WANG Kai-gong 《中国畜牧兽医》2017,44(12):3625-3633
In order to find out the serotype, resistant phenotype and genotype of Escherichia coli (E. coli) and Salmonella in piglets, this study collected 128 samples of diarrhea piglets from seven large-scale pig farms in five cities in Guizhou province, and the E. coli and Salmonella were isolated and identified. The pathogenicity of the strain was identified by animal test. The drug resistance of the main pathogen was tested by drug susceptibility paper. The resistance gene of each pathogen was detected by PCR. The drug resistance and genotype correlation of the bacterial were analyzed. The results showed that 78 strains of pathogenic E. coli and 21 strains of Salmonella were isolated and identified in this study. The serotypes of pathogenic E. coli were predominantly O138 and O87. Salmonella Typhimurium and Salmonella Enteritidis were predominant serotypes. The susceptibility test showed that the resistant strains of 78 strains of E. coli were more than 80% resistant to β-lactams and more than 40% for other antibacterials. The resistance rate of 21 strains of Salmonella to aminoglycosides was more than 50% and more than 20% to other types of antibacterials; 12 and 10 kinds of drug resistance-related genes of E. coli and Salmonella were detected, respectively; The coincidence rate of resistant genotype and phenotype of two kinds of bacteria were above 60%, and both were multiple drug resistance. This study provided a theoretical basis for comprehensive prevention and control of piglets diarrhea. 相似文献
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为探明仔猪细菌性腹泻肠道致病性大肠埃希氏菌和沙门氏菌流行的血清型、耐药表型及耐药基因型,本试验采集了贵州省5个地（州）市7个规模化养猪场的128份腹泻仔猪肠道样本,并对采集的样本进行了大肠埃希氏菌和沙门氏菌分离与鉴定,通过动物试验鉴定菌株的致病性,利用血清学方法鉴定其血清型,并通过药敏纸片法对主要致病菌进行耐药性研究,采用PCR技术检测各致病菌株耐药相关基因,分析细菌耐药表型和耐药基因型相关性。结果显示,本研究共分离鉴定到78株致病性大肠埃希氏菌与21株沙门氏菌,致病性大肠埃希氏菌血清型以O138、O87为主,沙门氏菌血清型以鼠伤寒沙门氏菌、肠炎沙门氏菌居多;药敏试验结果表明,本试验分离到的78株致病性大肠埃希氏菌对β-内酰胺类药物耐药率达80%以上,对其他种类的抗菌药耐药率均超过40%,分离鉴定的21株沙门氏菌对氨基糖苷类药物耐药率达50%以上,对其他种类的抗菌药耐药率均达20%以上;本试验分离鉴定的致病性大肠埃希氏菌共检出12种耐药相关基因,沙门氏菌共检出10种耐药相关基因,两种细菌耐药基因型与耐药表型符合率均达60%以上,且均为多重耐药。本研究为仔猪腹泻的综合防控提供了理论依据。 相似文献
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用从健康鸡消化道分离的鸡源乳酸杆菌４株,双歧杆菌２株,对引起雏鸡腹泻的鸡白痢沙门氏菌和鸡副伤寒沙门氏菌进行体外生物拮抗试验。试验结果显示：３株乳酸杆菌、２株双歧杆菌、１株芽孢杆菌对致病性沙门氏菌均有明显的生物拮抗作用（Ｐ＜０．０１）,而１株乳酸杆菌和１株芽孢杆菌拮抗作用不明显。 相似文献
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A total of 101 strains of Salmonella Enteritidis phage types (PT) 1, 4, 6, and 8 from Denmark, England and Spain were studied by PFGE to elucidate genetic relationships among strains isolated from animal, human and environmental sources between 1983 and 1997. Analysis with Xba I, Bln I and Spe I enzymes showed that the power of discrimination of this method was increased by the combination of the three enzymes (D=0.802), subdividing the strains into 28 genomic groups or genotypes. Many of the PT1, PT4, and PT6 strains from the three countries shared the same PFGE combination profile A1-A1-A1, confirming the close relationship among these phage types and the protracted spread of a single clone over a large geographical area. In general, strains from Denmark showed more variation in their PFGE profiles than those from England and Spain. PT4 strains exhibited genetic homogeneity in the three countries independently of their sources and period of isolation. Spe I gave the highest index of discrimination among PT6 strains as evidenced by a variety of PFGE profiles. The data clearly confirmed that PT8 strains isolated in the three countries were of a unique clonal origin, and the PFGE combination profile A10-A10-A1 was predominant and specific for this phage type. It is concluded that PFGE, in combination with phage typing, represents a suitable tool for the epidemiological typing of Salmonella Enteritidis strains which could be used for investigations or surveillance of the international spread of these clones. 相似文献
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旨在研究引起雏鹅痛风的新型鹅星状病毒流行株特征。本试验从安徽省鹅星状病毒阳性雏鹅组织中进行病毒分离,并对分离的毒株进行全基因组测序,随后进行遗传进化分析。结果表明,病毒液接种9~11日龄鹅胚可成功分离到病毒,将其命名为GASTV-ZM株。IFA （indirect immunofluorescence assay）鉴定为阳性。基因组序列分析表明,ZM株鹅星状病毒基因组全长7 175 nt,ORF2基因核苷酸和氨基酸与2014—2020年间分离毒株的同源性分别为96.7%~98.9%和96.9%~99.0%,ORF2基因编码的氨基酸序列结果显示,3个氨基酸突变位点与以往存在显著差异,ZM株与2016年以来在我国流行的新型鹅星状病毒处于同一进化分支,与2020分离株HNSQ-6株和XT1株同源性较高。本研究为进一步研究该病毒的致病性和致病机理奠定基础。 相似文献
18.
从湛江养鹅场发生的疑似大肠杆菌病病死鹅无菌采集病料,分离鉴定出8株大肠杆菌,其中,O86K61 4株,O44K74 3株,1株未定型,上述菌株均不产生内毒素;药敏试验结果表明,大部分菌株对阿米卡星高度敏感,对恩诺沙星、先锋霉素、磺胺嘧啶、诺氟沙星、强力霉素呈交叉耐药;对万古霉素、新霉素、呋喃唑酮中度敏感;对阿莫西林、链霉素、卡那霉素、庆大霉素、四环素不敏感。上述结果提示,同一发病鹅场存在着多种致病性大肠杆菌的血清型,并存在多重耐药。 相似文献
19.
Guth BE Chinen I Miliwebsky E Cerqueira AM Chillemi G Andrade JR Baschkier A Rivas M 《Veterinary microbiology》2003,92(4):335-349
Shiga toxin (Stx)-producing Escherichia coli (STEC) strains isolated from animals and food in Argentina (n=44) and Brazil (n=20) were examined and compared in regard to their phenotypic and genotypic characteristics to evaluate their pathogenic potential. The clonal relatedness of STEC O157 isolates (n=22) was established by phage typing (PT) and pulsed-field gel electrophoresis (PFGE). All O157 strains studied carried eae and enterohemorrhagic E. coli (EHEC)-hly sequences. In Argentina, these strains occurred both in cattle and meat, and 50% of them carried stx2/stx2vh-a genes, whereas in Brazil the O157 strains were isolated from animals, and most harbored the stx2vh-a sequence. At least 13 different O:H serotypes were identified among the non-O157 strains studied, with serotype O113:H21 being found in both countries. All but one non-O157 strains did not carry eae gene, but EHEC-hlyA gene was found in 85.7% of them, and the stx2 genotype was also more prevalent in Argentina than in Brazil (P<0.01), where stx1 alone or in association was most common (68.8%). One STEC strain isolated from a calf in Brazil harbored the new variant referred to as stx2-NV206. PFGE analysis showed that STEC O157 strains were grouped in four clusters. One Brazilian strain was considered possibly related (> or =80%) to Argentinean strains of cluster I. Differences in the pathogenic potential, especially in regard to serotypes and stx genotypes, were observed among the STEC strains recovered from animals and food in both countries. 相似文献
20.
为研究鹅细小病毒(GPV)基因遗传变异特征,采集海南某养鹅场疑似鹅细小病毒感染的病料,将其处理后接种番鸭胚成功分离到一株病毒,经PCR鉴定为鹅细小病毒,命名为HN株,并获得了其全基因组序列,将该序列与GenBank数据库中登录的16条鹅和番鸭细小病毒基因序列进行了比对分析。结果显示,该株病毒基因组全长为5 106bp,由ITR、NS、VP构成,其中ITR为444bp,NS1为1 844bp,VP1为2 199bp;HN株与SHFX1201株的NS1基因和VP1同源性最高,分别达到99.8%和99.7%,与番鸭细小病毒株FM的NS1基因同源性最低,为82.7%;与90-0215株VP1同源性最低,为80.1%。HN株的遗传进化树可以看出,GPV可以分成明显的2个基因亚群,HN株与鹅细小病毒匈牙利株(B)、欧洲疫苗株(VG32/1)和台湾株(82-0321V、82-0321、06-0329)均处在第I亚群,且与安徽分离株Y株以及SHFX1201株同源性最接近,番鸭源匈牙利株FM单独处于第Ⅱ亚群。本研究丰富了GPV的数据资料,为研究GPV分类地位以及遗传进化关系提供了依据,同时也为研究GPV流行趋势和疫苗的开发奠定了基础。 相似文献