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1.
不同激素剂量对幼鼠超数排卵及超排卵质量的影响   总被引:8,自引:0,他引:8  
应用国产激素制剂对3~8周龄昆明种小白鼠进行了超数排卵处理,重点观察了不同激素剂量对幼龄小鼠的超排效果及超排卵质量的影响。实验结果表明,以PMSG与hCG各10IU剂量超排3~8周龄小鼠,5周龄小鼠的超排效果(平均获卵83.8±10.2个)极显著(P<0.01)好于4~8周龄小鼠的超排效果(平均获卵39.2~49.8个),而3周龄小鼠超排卵受精后体外培养的卵裂率(97.1%)、桑椹胚率(86.8%)和囊胚率(65.1%)与4~8周龄超排卵的(分别为85.5%~100.0%、94.9%~100.0%和63.2%~87.7%)相近;以PMSG与hCG各5IU剂量超排3~5周龄小鼠,3周龄小鼠的超排效果(平均获卵59.0±6.5个)显著(P<0.05)好于4周龄小鼠的超排效果(平均获卵31.4±4.9个),并极显著(P<0.01)好于5周龄小鼠的超排效果(平均获卵21.4±3.3个);以PMSG与hCG各15IU剂量超排3~5周龄小鼠,3周龄小鼠的超排效果(平均获卵48.6±7.3个)显著(P<0.05)好于5周龄小鼠的超排效果(平均获卵25.2±5.8个),也好于4周龄小鼠的超排效果(平均获卵40.2±5.1个  相似文献   

2.
牛超排技术的研究   总被引:7,自引:1,他引:6  
用4组药物组合:(1)PMSG+PGF2a;(2)PMSG+PGF2a+激光照射;(3)FSH+PGF2a;(4)FSH+Ctoprostenol,超排处理322头次供体牛。共采卵1663枚(头均5.81±4.68),可用胚胎980枚(头均3.43±3.94,占58.9%)。(3)、(4)两组胚胎可用率及可用胚胎数高于(1)、(2)组(p<0.05);黄牛胚胎可用率高于奶牛(p<0.01);育成牛胚胎可用率高于经产牛(p<0.01);供体牛前两次超排效果更好(p<0.05)。  相似文献   

3.
对45头供体母牛随机用FSH+LRH-A3和FSH进行超然处理。其中FSH+LRH-A3处理供体52头次,FSH处理供体22头次;FSH十LRH-A3处理组平均获得如数、可用胚胎数分别是10.1±8.0枚,7.4±6.9枚,与FSH处理组(二指标分别是9.3±6.2枚,3.6±2.7枚)相比,分别高8.6%、105.6%,且二组超排获得可用胚胎数差异显著(P<0.05)。  相似文献   

4.
GnRH类似物LRH—A3配合FSH对超排母牛胚胎质量的影响   总被引:5,自引:0,他引:5  
对45头供体母牛随机用FSH+LRH-A3和FSH进行超排处理。其中FSH+LRH-A3处理供体52头次,FSH处理供体22头次;FSH+LRH-A3头处理组平均获得卵数,可用胚胎数分别是10.1±8.0枚,7.4±6.9枚,与FSH处理组(二指标分别是9.3±6.2枚,3.6±2.7)相比,分别高8.6%,105.6%,且二组超排获得可用胚胎数差异显著(P<0.05)。  相似文献   

5.
商品肉鸡在西宁地区生理常值的测定   总被引:2,自引:0,他引:2  
苏瑛  常忠平 《家畜生态》1994,15(4):32-33
对明星、AA肉仔鸡在海拔2200m处的西宁地区进行生理常值的测定,结果为:1.呼吸频率高于文献记录(P<0.05),体温、心率与之相似;2.明星和AA鸡红细胞数、血红蛋白及PCV分别为2.73±0.37和2.93±0.44百万/mm3,11.3±1.3和12.6±3.4g/L,43.6±3.9和41.7±4.3ml%,与文献记载均有差异(P<0.05);3.两品种的MCH、MCV分别为38.8±14.5和46.1±8.1pg,159.2±23.0和17.1±13.6U3,显著高于卢宗藩资料(P<0.05)。明星鸡MCHC低于王文三资料,AA鸡的高于其数据(P<0.05);4.白细胞分类计数表明,两品利的嗜酸、嗜碱、异嗜、淋巴和单核细胞均与资料有一定变化。  相似文献   

6.
家牦牛,半血野牦牛年产毛量的比较   总被引:1,自引:1,他引:0  
对176头各龄家牦牛和半血野牦牛的年产毛量进行了测定。测定结果:家牦牛1岁0.98±0.29kg/头,2岁1.12±0.22kg/头,3一5岁1.18±0.38kg/头,6岁以上0.88±0.27kg/头;半血野牦牛相应为0.90±0.20kg/头,1.05±0.27kg/头,0.68±0.28kg/头,0.90±0.38kg/头;3一5岁家牦牛组的年产毛量极显著高于6岁以上年龄组(P<0.01),同1岁、2岁年龄组的年产毛量差异不显著(P>0.05),2岁与6岁以上年龄组之间呈显著性差异(P<0.05);半血野牦牛各年龄组的年产毛量差异不显著(P>0.05);1岁家牦牛公母间,2岁半血野牦牛公母间,年产毛量差异极显著(P<0.01)。  相似文献   

7.
将41头产后40-100天卵巢静止奶牛分为四个处理组:OIUPMSG(n=12)、1000IUPMSG(n=12)、1250IUPMSG(n=12)及1500IUPMSG(n=5),处理后一周内的排卵率分别为0.0%、83.33%、91.67%及40.0%(P<0.01),从处理到妊娠间隔时间分别为89.1±36.7天、29.8±2且.7天、51.9±25.2天及60.2±29.6天(P<0.01);29头试验母牛又按产后40-60天及产后61-100天分为两组,处理后一周内的排卵率分别为92.9%及66.7%(P>0.05),从处理到妊娠间隔时间分别为50.3±27.0天及38.5±27.0天(P>0.05)。结果表明,PMSG处理卵巢静止母牛具有明显效果,其中以1000IU为最优,处理时间的早晚对处理效果无明显影响。  相似文献   

8.
运输性应激对商品猪影响的研究   总被引:2,自引:0,他引:2  
商品猪720头,每头体重75~100kg。按常规行车,经历2小时,行程50km的公路汽车运输试验观测.在运输过程中猪只表现兴奋、不安和互相干扰、咬斗等现象。运输结束后表现精神沉郁和食欲不振。对猪的生理状态影响显著,心博率显著加快;运输前平均为75.5±4.6次/分钟,运输后平均为99.23±14次/分钟(P<0.01);呼吸率显著增加,运输前平均为12.25±3.4次/分钟.运输后平均为24.3±3.2次/分钟.(P<0.01);体温明显增高.运输前平均为39.3±0.3℃,运输后平均为39.98±0.5℃(P<0.05)。体重耗损较大.运输前平均为88.5±3.1kg,运输后平均为84.7±2.5kg,耗重率平均为4.3%。病残猪为25头(占3.4%),死亡8头(占1.1%).共计为4.5%。  相似文献   

9.
半胱胺对奶牛产奶量及血浆生长抑素、生长激素水平的影响   总被引:42,自引:1,他引:41  
选用10 头处于泌乳中期的荷斯坦牛进行自身对照试验。对照期饲喂基础日粮,试验期在基础日粮中添加半胱胺。每隔5 天一次,剂量为100mg/kg 体重,共喂3 次。结果表明,试验期奶牛日产奶量比对照期提高7 .6 % ( 对照期14 .5 ±3 .9kg/ 头,试验期15 .6 ±4 .3kg/ 头P< 0 .05) ;奶牛采食量无明显变化,试验期饲料转化率比对照期明显提高( 对照期0 .32 ±0 .01 ,试验期0 .30 ±0 .02 ,P< 0 .01) ;与对照期相比,试验期奶牛血浆生长抑素(SS) 水平明显下降( 对照期1 .21 ±0 .34ng/ml,试验期0 .14 ±0 .05ng/ml,P( < 0 .01) ,生长激素含量显著提高( 对照期1 .33 ±0 .26ng/ml,试验期1 .77 ±0 .29ng/ml,P< 0 .05) ;半胱胺对牛乳脂率无明显影响( 对照期2 .16 ±0 .58 ,试验期2 .53 ±0 .06) 。以上结果表明,半胱胺能明显提高奶牛产奶量,而抑制体内生长抑素,提高内源性生长激素水平,可能为其主要的作用机制。  相似文献   

10.
在生长肥育猪日粮中添加高铜(187.90±2.75mg)和超高铜(367.90±0.500mg),结果每千克肥育猪眼肌肉中铜元素平均值为10.80±0.23mg和11.30±0.11mg,比对照组增加了2.3倍和2.4倍差异显著(P〈0.05),肝脏中平均值为154.90±4.30mg和407.5±5.3mg,比对照组增加30.73倍和3.57倍,差异极显著(P〈0.01)。特高铜组特出现肝脏肿大  相似文献   

11.
12.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。  相似文献   

13.
采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。  相似文献   

14.
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。  相似文献   

15.
为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …  相似文献   

16.
以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。  相似文献   

17.
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.  相似文献   

18.
乳酸杆菌作为益生菌广泛用于人和动物.本文综述了乳酸杆菌改善宿主健康的机制.乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道.文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制.  相似文献   

19.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.  相似文献   

20.
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