首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 437 毫秒
1.
为了建立超快速液相色谱-串联质谱法同时测定枳实中6个活性成分(异柚皮苷、柚皮苷、橙皮苷、新橙皮苷、柚皮素和橙皮素)的含量,采用Shim-Pack XR-ODS柱(75 mm×3.0 mm,2.2μm),以0.1%甲酸水溶液(A)-乙腈(B)为流动相,梯度洗脱(0～10 min,10%→30%B;10～12 min,30%→60%B;12～15 min,60%→85%B),平衡时间为3 min,流速为0.5 mL/min,柱温为30℃,进样量为5μL。质谱采用3200 QTRAP三重四极杆串联质谱仪,离子源为电喷雾电离源(ESI源),检测方式为负离子检测,扫描模式为多重反应检测(MRM)。结果显示,异柚皮苷、柚皮苷、橙皮苷、新橙皮苷、柚皮素和橙皮素浓度分别在0.2～8.0μg/mL(r=0.999 2)、1.0～40μg/mL (r=0.999 4)、0.2～8.0μg/mL (r=0.999 7)、0.5～20μg/mL(r=0.999 2)、0.02～0.8μg/mL (r=0.999 7)和0.02～0.8μg/mL (r=0.999 7)浓度范围内与峰面积呈现良好的线性关系;平均回收率(n=9)分别为99.2%、98.7%、98.6%、98.8%、99.3%和99.1%。6批枳实样品测定结果显示:异柚皮苷1.70%～1.97%,柚皮苷11.6%～12.4%,橙皮苷0.481%～0.576%,新橙皮苷3.15%～4.13%,柚皮素0.293%～0.391%和橙皮素0.363%～0.395%。表明该方法快速、准确、重复性好,可用于枳实的质量控制,同时为新兽药开发利用提供参考。  相似文献   

2.
建立高效液相色谱法同时分离测定复合有机酸化剂中的烟酸、乳酸、柠檬酸、苯甲酸、水杨酸含量.采用Agela-ASB(r)C18柱(250 mm×4.6 mm,5μm);盐酸溶液(0.02%,V/V,A)和乙腈(B)为流动相,梯度洗脱;流速1.0mL/min;检测波长210 mn;柱温:(35±2)℃.在此色谱条件下,5种有机酸分离良好,它们的峰面积响应与浓度分别为烟酸1～20 μg/mL;乳酸100～2000 μg/mL;柠檬酸100～2000μg/mL;苯甲酸2.5～50μg/mL;水杨酸2.5～50μg/mL,线性关系良好(r>0.9995).平均加样回收率均分别为99.1%,98.2%,97.2%,96.3%,96.0%;RSD 小于1.0%.该方法操作简便易行,系统适用性良好,方法准确可靠.  相似文献   

3.
建立高效液相色谱法同时分离测定复合有机酸化剂中的甲酸、丙酸、乳酸、马来酸、柠檬酸、富马酸、苯甲酸含量。采用Agela-ASBC18柱（250mm×4.6mm,5μm）;盐酸溶液（0.1%,V/V,A）和乙腈（B）为流动相,梯度洗脱;流速1.0mL/min;检测波长210nm;柱温（30±2）℃。在该色谱条件下,7种有机酸分离良好。它们的峰面积响应与浓度分别为：甲酸100～2000μg/mL、乳酸100～2000μg/mL、马来酸2～40μg/mL、柠檬酸100～2000μg/mL、富马酸2～40μg/mL、丙酸100～2000μg/mL、苯甲酸2～40μg/mL范围内,线性关系良好（r＆gt;0.9995）。平均加样回收率分别为99.1%、98.6%、100.1%、98.8%、99.3%、99.5%、98.2%,RSD小于1.5%。该方法操作简便易行,系统适用性良好,方法准确可靠。R  相似文献   

4.
气相色谱法测定兽药饲料添加剂中的维生素E含量   总被引：1，自引：0，他引：1  
采用气相色谱法测定亚硒酸钠维生素E注射液及预混剂中维生素E含量。将注射液用1.0mL盐酸溶液(1→4)及8～10mL无水乙醇进行破乳,预混剂用正己烷加热回流提取法处理后,以正三十二烷为内标,色谱柱:硅酮(OV-17)为固定相,硅藻土为担体,涂布浓度为2%的不锈钢柱(3mm×2m),氮气流速60mL/min,氢火焰离子化检测器(FID)进行分析。方法的线性范围为0.30mg/mL～6.00mg/mL(r=0.9994),注射液和预混剂方法回收率分别为99.16%～100.14%,99.73%～100.48%,日内和日间相对标准偏差,注射液分别为1.61%～2.10%,1.05%～1.94%;预混剂分别为0.91%～1.03%,0.88%～1.14%。方法准确,可靠,分析结果令人满意。  相似文献   

5.
为了建立超快速液相色谱法同时测定栀子厚朴汤中京尼平苷和京尼平1-β-D龙胆双糖苷的含量,采用Shim-Pack XR-ODS柱(75 mm×3.0 mm,2.2μm),流动相为0.1%甲酸水溶液(A)-乙腈(B),梯度洗脱(0～5 min,7%B→12%B;5～11 min,12%B→13%B),平衡时间为2 min,流速为0.4 mL/min,检测波长为240 nm,柱温为30℃,进样量为5μL。结果显示:京尼平苷和京尼平1-β-D-龙胆双糖苷分别在10～500μg/mL(r=0.999 7)和1～50μg/mL(r=0.999 9)浓度范围内与峰面积呈良好的线性关系;平均回收率(n=9)分别为98.2%和97.8%。6批样品中上述2个成分的含量范围分别为5.13%～5.84%和1.75%～2.82%。表明该方法快速准确、操作简便,具有良好的稳定性和重复性,可为栀子厚朴汤的质量控制提供依据,同时为新兽药开发利用提供参考。  相似文献   

6.
本试验旨在优选大米活性肽的喷雾干燥工艺。采用L9(34)正交试验设计,以喷干粉得率和含水量为指标,考察了进风温度、进样速度和离心雾化器转速对大米活性肽喷雾干燥工艺的影响。结果表明:当进风温度为200℃、进样速度为45mL/min和离心雾化器转速为15 000r/min时,大米活性肽(89.62%)得率达到最高;当进风温度为200℃、进样速度为30mL/min和离心雾化器转速为25 000r/min时,大米活性肽(7.46%)含水量达到最低。综合各项指标的分析,最佳的喷雾干燥工艺参数为进风温度200℃、进样速度45mL/min和离心雾化器转速15 000r/min。  相似文献   

7.
本文建立了测定复方氯硝柳胺片中氯硝柳胺和左旋咪唑的高效液相色谱法,采用Eclipse plus C-18(4.6×250 mm,5μm)色谱柱,流动相为甲醇:0.05 mol/L乙酸铵(70∶30,V/V),流速为1.0 mL/min,紫外检测波长为220 nm,柱温35℃。结果显示,氯硝柳胺在10μg/mL～800μg/mL,左旋咪唑在1μg/mL～80μg/mL范围内,浓度与峰面积积分值呈良好的线性相关,相关系数r分别为0.9998、0.9999;氯硝柳胺和左旋咪唑低、中、高三种浓度的平均回收率均在95.42%～101.12%之间,RSD在0.31%～1.48%之间;氯硝柳胺和左旋咪唑的定量限分别为1μg/mL、0.5μg/mL,检测限分别为0.4μg/mL和0.2μg/mL。结果表明,该方法快速、简洁、专属性强,回收率高,稳定性良好。  相似文献   

8.
建立了测定安痛定注射液中氨基比林和安替比林含量的高效液相色谱法.采用Waters公司Symmetry C18 ( 4.6 mm ×250 mm,5μm)色谱柱;以甲醇-水(40∶ 60)为流动相;检测波长为260 nm;流速为1.0 mL/min;柱温25 ℃.结果表明:氨基比林在20～200 μg/mL的浓度范围内呈线性关系,r=0.999 8,平均回收率为100.4%;安替比林在10～60 μg/mL的浓度范围内呈线性关系,r=0.999 7,平均回收率为100.2%.该法简便、准确可靠,可应用于安痛定注射液的质量控制.  相似文献   

9.
建立了羟基甲硝唑对照品中残留溶剂甲醇和丙酮的顶空气相色谱测定方法。采用Agilent-DB624石英毛细管色谱柱(30 m×0.53 mm,1.0μm);以氮气为载气,氢火焰离子化检测器为检测器,进样口温度200℃,检测器温度250℃;以起始温度30℃,保持5 min,20℃/min升温至200℃,保持2 min为程序升温方法;以顶空瓶平衡温度80℃,平衡30 min为顶空条件。结果表明,在该色谱条件下甲醇和丙酮与其他物质分离良好。甲醇在2～360μg/mL范围内线性关系良好,检测限为1.0μg/mL;丙酮在1～600μg/mL范围内线性关系良好,检测限为0.5μg/mL。甲醇回收率为98.0%～102.4%,RSD为0.6%～0.9%;丙酮回收率为97.0%～99.6%,RSD为0.6%～1.0%。本方法操作简单,准确度高,重复性好,可用于羟基甲硝唑中残留溶剂的检测。  相似文献   

10.
高效液相色谱法测定鸡肝组织中的氢溴酸常山酮残留   总被引：2，自引：2，他引：2  
用高效液相色谱法测定鸡肝组织中常山酮的残留量.色谱柱为Kromasil C18(250 mm×4.6 HDM, 5 μm);流动相为乙腈-醋酸盐缓冲液(0.25 mol/L,pH 4.3)-水(5∶3∶12),检测波长243 nm,流速2.0 mL/min.该方法线性范围为0.10～5.00 mg/mL,r=0.999 9,平均回收率达到77.0%～80.3%,RSD<4.3%.该方法灵敏、准确、重现性好.  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

14.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

15.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

16.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

17.
The purpose of this study was to determine the frequency of different tumor types within a large cohort of cats with intracranial neoplasia and to attempt to correlate signalment, tumor size and location, and survival time for each tumor. Medical records of 160 cats with confirmed intracranial neoplasia evaluated between 1985 and 2001 were reviewed. Parameters evaluated included age, sex, breed, FeLV/FIV status, clinical signs, duration of signs, number of tumors, tumor location(s), imaging results, treatment, survival times, and histopathologic diagnosis. Most of the cats were older (11.3 +/- 3.8 years). Primary tumors accounted for 70.6% of cases. Metastasis and direct extension of secondary tumors accounted for only 5.6 and 3.8% of cases, respectively. Twelve cats (7.5%) had 2 or more discrete tumors of the same type, whereas 16 cats (10.0%) had 2 different types of intracranial tumors. The most common tumor types were meningioma (n = 93, 58.1%), lymphoma (n = 23, 14.4%), pituitary tumors (n = 14, 8.8%), and gliomas (n = 12, 7.5%). The most common neurological signs were altered consciousness (n = 42, 26.2%), circling (n = 36, 22.5%), and seizures (n = 36, 22.5%). Cats without specific neurological signs were common (n = 34, 21.2%). The tumor was considered an incidental finding in 30 (18.8%) cats. In addition to expected relationships (eg, meninges and meningioma, pituitary and pituitary tumors), we found that lesion location was predictive of tumor type with diffuse cerebral or brainstem involvement predictive of lymphoma and third ventricle involvement predictive of meningioma.  相似文献   

18.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

19.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

20.
Comparison of different tests to diagnose feline infectious peritonitis   总被引：1，自引：0，他引：1  
  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号