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1.
湖北省羊口疮病毒的分离鉴定   总被引：1，自引：0，他引：1  
利用MDBK传代细胞系对湖北通山县某羊场疑似羊口疮(Orf)的黑山羊病料进行分离,通过病毒理化特性试验、动物回归试验和PCR等方法对分离的毒株进行鉴定。结果表明,病料悬液接种MDBK细胞后出现细胞病变,接种病料的羊只出现典型的Orf症状,扩增出羊口疮病毒(Orf virus)B2L基因,该毒株与台湾山羊株(EU935106.1、DQ904351.1)相似性最高(均为99%),亲缘关系最近。本研究成功分离鉴定到一株ORFV,命名为ORFV/HB/09。  相似文献   

2.
本试验利用犊牛睾丸细胞、羔羊睾丸细胞、MDBK、BHK-21细胞对采自内蒙古赤峰的疑似羊口疮发病羊群的唇部痂皮组织进行接种传代,分离出1株病毒,通过透射电镜负染观察和PCR检测对该分离株进行鉴定。参照GenBank中羊口疮病毒(Orf virus,ORFV)ORF059 (F1L)基因的核苷酸序列,设计并合成1对特异性引物,成功地对分离株的F1L基因进行克隆、测序,并与多株参考毒株进行了同源性比对分析,结果显示经透射电镜负染观察到典型的副痘病毒粒子,与参考毒株序列相比均具有较高的同源性,均为96%以上,结果表明该分离株为ORFV,命名为OV/nm-hd。  相似文献   

3.
利用犊牛睾丸原代细胞对山东省威海市某奶山羊养殖场疑似羊口疮（Orf）山羊病料进行病毒分离,通过细胞病变观察、病毒滴度和聚合酶链反应（PCR）等方法对分离毒株进行鉴定。结果表明,研磨处理的病毒液接种于犊牛睾丸原代细胞后盲传至第5代出现病变,测得第6代分离株病毒滴度为106．5 TCID50／mL。扩增羊口疮病毒特异性 B2L 基因,发现该毒株与34个羊口疮毒株核苷酸序列同源性高达99％,包括福建株（KC588399．1、KC568398．1）。本研究成功分离鉴定了羊口疮病毒山东威海株。  相似文献   

4.
新疆羊口疮病毒分离鉴定及B2L基因分析与表达   总被引：1，自引：0，他引：1  
为研究新疆地区羊口疮(Orf)病毒(ORFV)的生物学特性及流行特征,本研究采集新疆地区疑似Orf的羔羊结痂病料,用MDBK传代细胞进行病毒分离培养及电镜观察,进行动物回归实验;对ORFV B2L基因进行PCR扩增,并构建B2L基因原核表达重组质粒pET-32a-B2L,转化至大肠杆菌BL21.将表达产物进行SDS-PAGE及western blot检测,结果证明所获得的分离株ORFV-shz为ORFV,与India 67/04分离株的亲缘关系最近,其重组B2L蛋白大小为60 ku,并具有良好反应原性.  相似文献   

5.
羊口疮(Orf)是由羊口疮病毒(ORFV) 引起的一种急性、接触性和高度嗜上皮性人畜共患的动物传染病,主要感染绵羊和山羊,有时威胁人类。本研究将ORFV(OV/HLJ/04株)纯化后作为试验材料,采用巨噬细胞系RAW264.7作为病毒细胞宿主,RAW264.7在LPS体外激活的情况下,接种ORFV,研究ORFV感染对巨噬细胞凋亡的影响。结果表明,用LPS处理的试验组巨噬细胞形态学改变较大,凋亡量也较大;而没用LPS处理过的试验组巨噬细胞变化则相对较少。Western blotting研究结果显示,细胞凋亡信号Caspase-3与甘油醛-3-磷酸脱氢酶(GAPDH)在24 h时对比,凋亡信号明显减弱,表明在24 h的巨噬细胞凋亡水平有所下降。  相似文献   

6.
利用初生胎羊鼻甲骨(Primary ovine fetal turbinate,OFTu)细胞从送检的疑似“口疮”症状的病羊唇部痂皮中分离获得1株病毒,用PCR检测、电镜负染观察及理化性质测定等方法对该分离株病毒进行鉴定,证实该分离株为羊传染性脓疱病毒(Off virus,ORFV),命名为(Orf virus Jilin-Nongan,ORFV JL-NA株).对ORFV JL-NA分离株Orf059(F1L)基因进行克隆、测序并对测序结果进行序列分析.结果显示,获得的ORFV JL-NA分离株Orf059(F1L)基因序列长为994 bp,编码329个氨基酸,与ORFV-D1701株、ORFV-Jilin株、ORFV-NZ2株、ORFV-20株、ORFV-7株、ORFV-C2株、ORFV-IA82株、ORFV-SA00株、ORFV-mi90株、ORFV-torino株、ORFV-Shanxi株核苷酸序列同源性分别为96.4％、97.3％、99.1％、98.7％、99.0％、97.5％、98.4％、97.2％、97.4％、96.9％和96.8％.遗传进化树结果显示,ORFV JL-NA分离株与意大利ORFV-7毒株的亲缘关系较近,表明不同地区分离株的Orf059(F1L)基因差异不大.  相似文献   

7.
《中国兽医学报》2019,(5):860-866
采用羊胚胎鼻甲(OFTu)细胞对湖北某山羊养殖场疑似羊口疮(Orf)的病羊唇部痂皮组织进行病毒分离,通过病理组织学、电镜形态观察及PCR试验等方法对分离的病毒进行鉴定,证实分离毒株为羊口疮病毒(Orf virus,ORFV),命名为HuB13。分析HuB13 F1L基因序列,结果F1L基因全长为1 017 bp,编码339个氨基酸,G+C含量为64.70%。遗传进化树显示,HuB13与8株山羊和6株绵羊来源的ORFV同源性分别为97.50%～99.41%和97.15%～97.35%,与牛痘病毒(PCPV)和牛丘疹性皮炎病毒(BPSV)同源性分别为88.0%～88.69%和75.15%。Bioedit软件分析发现宿主为山羊和绵羊的ORFV存在1个高变异区(148～171 bp)和6个特异性位点G186C、T187G、A541G、A765G、G856A和G982A。研究结果为开发宿主特异的Orf疫苗及致病机理研究提供参考依据。  相似文献   

8.
本试验通过对羊传染性脓疱病毒内蒙分离株(OV/nm-05)进行总DNA提取,参考GeneBank中OrfVirus(Strain NZ2)株F1L基因序列设计一对引物,应用PCR技术特异性地扩增出该毒株的F1L基因的片段,并将其克隆到pGEM-Teasy载体后进行测序,得到该病毒F1L基因序列。应用计算机软件将测定序列与参考毒株OV/7、Strain NZ2、OV/C2、OV/mi-90、OV/Torino、OV/2的F1L基因序列进行比较。结果表明羊传染性脓疱病毒内蒙分离株与Strain NZ2同原性最高,达99.1%,与OV/Torino最低,但也为96.3%。说明羊传染性脓疱病毒内蒙分离株F1L基因与其他参考毒株之间差异不大。  相似文献   

9.
利用原代犊牛睾丸细胞从临床上表现口疮症状的羔羊痂皮材料中分离获得1株病毒,对该株病毒进行病毒形态学、理化学、PCR检测与动物回归试验等系统鉴定后,证实该分离株为羊传染性脓疱病毒(Orf virus,ORFV),命名为()RFV-sy.利用PCR方法克隆出其B2L基因,并将该基因序列和推导的氨基酸序列与6个不同来源的ORFV毒株进行同源性和亲缘关系的比较分析.结果表明,各毒株间核苷酸和氨基酸的同源性分别为97.8%～99.5%和97.6%～99.5%;系统发育进化树结果表明,ORFV-sy毒株与印度分离绵羊株ORFV-Mukteswar 67/04、ORFV-Izatnagar 79/04的亲缘关系较近,表明不同地区分离毒株的B2L基因差异不大.  相似文献   

10.
羊口疮(Orf)是由羊口疮病毒(Orf virus,ORFV)引起的一种接触性传染病,绵羊与山羊是主要宿主.该病属于自限性疾病,当不发生继发性感染时,机体调节就可以控制疾病发展,逐渐恢复痊愈.该特性可能与病毒的基因组结构有关,因此,重点阐述了羊口疮病毒基因组编码的蛋白及其功能.此外,还对羊口疮病毒的流行病学、病原学、临...  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
Tropical Animal Health and Production -  相似文献   

14.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

15.
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.  相似文献   

16.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

17.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

18.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

19.
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors.  相似文献   

20.
<正>1.INTRODUCTION Ensuring transportation network security is one Of the most daunting challenges confronting homeland security agencies today.Significant research has been dedicated.To model and analyze the vulnerability of transportation systems,while notably fewer studies propose specific strategies for deploying defensive technologies to safeguard these systems.  相似文献   

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