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针对现有水产设备机械化和自动化程度较低,增氧机作用范围有限和投饲机无法自适应投饲的问题,研究设计了一种新型的基于实时水质参数的智能养殖装备。该装备硬件上利用传感器对水质参数进行实时监测,采用太阳能与交流电源混合供电。其中,移动式太阳能增氧机使用超声波测距进行避障,可随机行走、增大增氧机的工作范围;太阳能智能投饲机使用称重传感器进行饲料称重,以实现精确定量投饲。该装备软件上支持个人计算机和手机等多个平台客户端,实现实时水质参数查询、远程增氧、远程投饲、远程智能控制等功能。池塘应用试验结果表明,该装备的监测水质数据可信度高,实时通讯丢包率低于0.2%,在保证增氧能力的情况下,增氧机作用范围比传统水车式增氧机提高10%;能够在良好的水质环境中完成精确定量投饲。研究表明,该装备的应用有助于推进水产设备智能化、自动化的发展,实现节能降耗、绿色环保的目标。 相似文献
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水车式增氧机中太阳能的应用研究 总被引：2，自引：0，他引：2
根据目前我国增氧机动力设备的配置情况和布置型式,通过对太阳电池利用的可行性分析,提出增氧机太阳能供电系统方式,并在比较各种增氧机结构、性能的基础上,选择水车式增氧机作为研究应用对象,提出了双车岸置固定式系统方案、双车机置固定式系统方案和单车机置移动式系统方案。实现节约电能、减少环境污染的目标,同时也为太阳电池在水产业中的应用提供了一条途径。 相似文献
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研发一种大水体太阳能自动增氧装置,为大水体的缺氧、水体污染提供一种解决方法。太阳能自动增氧装置由太阳能光伏发电系统、检测与智能增氧系统、自动化驱动系统组成。光伏发电系统充分利用太阳能资源,解决了电能消耗问题;检测与智能增氧系统实现了增氧过程中氧溶解浓度检测和智能感应运行;自动化驱动系统通过智能感应信号和电子差速控制系统实现增氧机原地转向、转弯和直行3种运动模式的移动,增加了增氧面积。使用太阳能自动增氧装置增氧试验表明,80 min内1 m水深处溶氧量增加0.79 mg/L,2 m水深处溶氧量增加0.78 mg/L,3m水深处溶氧量增加0.77 mg/L,4 m水深处溶氧量增加0.78 mg/L;改善水质试验表明能有有效提高水体溶氧,降低氮磷含量;养殖试验表明,增加鲤产量35.3%、鲢鳙产量31.2%。 相似文献
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针对水产养殖存在的自动化水平低、增氧设备耗能高以及太阳能利用技术普及不足等现状,设计了一套以太阳能为主要动力来源的水产养殖智能增氧系统。通过研究太阳能供电系统各部分的组成结构、运行方式及特点,结合选定地点的太阳能资源情况,分析不同情形下太阳能的辐射强度,确定光伏阵列容量,计算系统每日发电量与负载用电量的匹配情况,以达到太阳能电池板容量的优化配置,并将其应用于智能供氧系统。系统采用基于ATmega128单片机的硬件电路,以及软件程序设计,运用电导增量法、三阶段式充电法、逆变电路等技术实现最大功率点的跟踪、蓄电池的智能充放电、逆变器SPWM控制、供电源自动切换和增氧设备自动启停等主要功能。结果显示,该系统能有效提高鱼塘增氧效率、降低养殖成本,实现水产养殖的环保化和自动化。结果表明:该系统运行稳定、可靠、节省电能,能提高水体溶氧,可满足节能、环保的要求。 相似文献
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针对我国水产养殖自动化水平低、增氧设备耗能高以及太阳能发电技术普及不足等现状,设计了一套以太阳能为主要动力来源的水产养殖智能增氧控制系统。该系统采用基于ATmega128单片机的硬件电路和软件程序的设计,运用电导增量法、三阶段式充电法、逆变电路等技术,实现了最大功率点的跟踪、蓄电池的智能充放电、逆变器SPWM控制、供电源切换、增氧设备自动启停等主要功能。经样机试点测试表明,该系统运行稳定、可靠,能提高养殖池塘的增氧效率,满足节能、环保的要求。 相似文献
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典型增氧设备在养殖池塘中组合应用的研究 总被引：1，自引：0，他引：1
叶轮式、水车式、射流式和曝气式增氧机是目前我国池塘养殖使用的主要增氧设备;由于结构形式和工作原理的不同,4种形式的增氧机有不同的特点和功能。为提高养殖池塘增氧设备的增氧效果,通过增氧设备对养殖池塘水体不同深度增氧效果的试验和养殖池塘自然增氧的试验,分析了4种典型的增氧设备的增氧性能和特点,提出了叶轮式增氧机与耕水机、水车式增氧机与耕水机、水车式增氧机与射流式增氧机以及曝气增氧机与耕水机组合配置使用的混合增氧模式,可以优势互补,充分发挥各种形式增氧设备功能。通过组合使用,达到对养殖池塘水体最大限度的增氧效果的目的。 相似文献
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水产养殖常用的增氧设备主要有叶轮式增氧机、水车式增氧机、流射式增氧机、喷水式增氧机和曝气增式氧机等。叶轮式或水车式增氧机主要是设置在水体上层,单独使用很难满足养殖池塘的立体增氧要求,而且能耗相对较高。曝气增氧又可细分为气石曝气增氧和微孔管曝气增氧两种,其区别在于气体的扩散器, 相似文献
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移动式水车增氧机介绍 总被引：1，自引：0，他引：1
移动式增氧机在我国八十年代初就开始了研究，并取得了进展。但多数都是以几台叶轮式增氧机作为旋转动力、一边旋转．一边增氧。这样效果虽好，但电缆缠绕的问题始终没有得到解决。本文采取水平来回移动的办法，可避免电缆缠绕、实现移动增氧的目的。虽增氧效果和移动范围不如前者，但一般的中型鱼池中增氧效果还是可以的。１ 基本结构１．１ 电动机：功率：２～３ｋｗ左右。可正反向旋转，由控制器控制。１．２ 减速器：采用二级齿轮减速器．驱动中轴叶轮旋转、另两轴叶轮分别通过链条和链轮传动。齿轮减速器的润滑采用油池飞溅润滑；链条… 相似文献
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移动式微孔曝气增氧机是由江苏江阴市百川增氧设备有限公司生产的专利产品,于2012年开始推向市场。这种新型增氧机最大的特点是可随时移动增氧位置,不需铺没管道,用户可自行安装,节省安装成本。 相似文献
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Liping Li Rui Wang Wanwen Liang Xi Gan Ting Huang Yan Huang Jian Li Yunliang Shi Honglin Luo Ming Chen 《广西水产科技》2014,(1)
Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China. 相似文献
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Growth hormone (GH) and reproduction: a review 总被引：5，自引：0，他引：5
Florence Le Gac Odile Blaise Alex Fostier Pierre-Yves Le Bail Maurice Loir Brigitte Mourot Claudine Weil 《Fish physiology and biochemistry》1993,11(1-6):219-232
Interaction between growth and reproduction occurs in many vertebrates and is particularly obvious at certain stages of the life cycle in fish. Endocrine interactions between the gonadotropic axis and the somatotropic axis are described, the potential role of GH being emphasised. A comparative analysis of these phenomena in mammals, amphibians and fish, suggests a specific role of GH in the physiology of puberty, gametogenesis and fertility. It also shows the original contribution made by studies on the fish model in this field of investigations. 相似文献
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Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL?1 (1.25 ng well?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation. 相似文献
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Controlled infection of Poecilia reticulata Peters (guppy) with Tetrahymena by immersion and intraperitoneal injection 
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下载免费PDF全文 G Sharon M Pimenta‐Leibowitz M C L Vilchis N Isakov D Zilberg 《Journal of fish diseases》2015,38(1):67-74
Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality). 相似文献
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Iciar Martinez Amaya Velasco Ricardo Pérez-Martín Eskil Forås Michiaki Yamashita Carmen G. Sotelo 《Journal Of Aquatic Food Product Technology》2013,22(4):289-297
The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus. 相似文献
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The endemic, anadromous cyprinidChalcalburnus tarichi is the only fish species known to occur in alkaline Lake Van (Eastern Anatolia, Turkey). EightC. tarichi were maintained individually in Lake Van water (17 – 19°C; pH 9.8; 153 mEq·I–1 total alkalinity; 22 total salinity) and tank water samples analyzed for 24 h in 2 to 4 h intervals. At zero time, < 1µM ammonia was present and urea was undetectable in the tank water; at 24 h, total ammonia and urea made up 114±32 and 35±25µM, respectively. Over the experimental period, ammonia-N and urea-N excretion averaged 1041±494 and 607±169moles·kg–1 fish·h–1, respectively. The extent of urea excretion was highly variable between specimens. Uric acid excretion was not detectable.Urea was present at high concentrations in all tissues and plasma (25 – 35moles·g–1·ml–1) of freshly caughtC. tarichi; total ammonia content of the tissues was by a factor of 1.9 (liver) to 3.0 (brain) lower. High arginase activity (2.4±0.2 U·min–1·g–1) was detected in the liver ofC. tarichi but ornithine carbamoylphosphate transferase, a key enzyme of the ornithine-urea-cycle, was absent. Ureagenesis is likely through degradation of arginine and/or uricolysis. High glutamine synthetase activity (11±0.6 U·min–1·g–1) and low ammonia content in brain suggest that, like other teleosts,C. tarichi has an efficient ammonia detoxification in the brain, but in no other tissue.Nitrogenous waste excretion at alkaline pH is discussed. The ability ofC. tarichi to excrete high levels of ammonia at extremely alkaline pH is unique among teleosts studied so far. The mechanism of ammonia excretion under Lake Van conditions remains to be elucidated. 相似文献
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There has been growing concern about the overuse of antibiotics in the ornamental fish industry and its possible effect on the increasing drug resistance in both commensal and pathogenic organisms in these fish. The aim of this study was to carry out an assessment of the diversity of bacteria, including pathogens, in ornamental fish species imported into North America and to assess their antibiotic resistance. Kidney samples were collected from 32 freshwater ornamental fish of various species, which arrived to an importing facility in Portland, Oregon from Colombia, Singapore and Florida. Sixty‐four unique bacterial colonies were isolated and identified by PCR using bacterial 16S primers and DNA sequencing. Multiple isolates were identified as bacteria with potential to cause disease in both fish and humans. The antibiotic resistance profile of each isolate was performed for nine different antibiotics. Among them, cefotaxime (16% resistance among isolates) was the antibiotic associated with more activity, while the least active was tetracycline (77% resistant). Knowing information about the diversity of bacteria in imported ornamental fish, as well as the resistance profiles for the bacteria will be useful in more effectively treating clinical infected fish, and also potential zoonoses in the future. 相似文献
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Effect of iodophor disinfection of non‐hardened Salmo trutta eggs on their bacterial and fungus load 下载免费PDF全文
下载免费PDF全文 Franz Lahnsteiner 《Aquaculture Research》2017,48(7):3901-3909
This study investigated the efficiency of iodophor disinfection (135 ppm active iodine for 15–30 min) of non‐hardened Salmo trutta eggs against different groups of bacteria and against fungus. Egg samples were taken from non‐disinfected and from disinfected eggs, microorganisms were cultured on specific nutrient media and their mass was measured by turbidimetric methods. Bacteria and fungus mass of non‐hardened eggs could be reduced but not eliminated by iodophor disinfection with 135 ppm active iodine for 15 min. The extent of reduction was 47–65% (Experiment 1). The efficiency of disinfection increased with disinfection time as the reduction in bacteria and fungus mass was 40–55% after 15 min and 58–74% after 30 min (Experiment 2). Disinfection efficiency of iodophor solution diluted in water (reduction 49–57%) and of iodophor solution diluted in sodium chloride solution iso‐osmolar to the oocytes (reduction 52–61%) was similar (Experiment 3). The reduction in bacteria and fungus mass was persistent as it was 39–72% lower in embryos deriving from disinfected eggs than in embryos deriving from non‐disinfected ones (Experiment 4). In conclusion, the tested disinfection method is inadequate to eliminate pathogens completely but it could positively influence immune defence of eggs and embryos. 相似文献