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1.
白粉病是青稞生产中危害最为严重的病害之一,会造成产量严重下降。本实验室前期筛选到一个白粉菌诱导后上调表达的NF-YB转录因子HVUL0H33228.2。为了进一步明确该基因的作用机制,本研究构建了青稞受白粉菌诱导的酵母双杂交cDNA文库,以HVUL0H33228.2为诱饵进行酵母双杂交筛选。结果表明：所获得的cDNA文库滴度为5.6×108CFU/mL,插入片段长500～2 000 bp,重组率为94%,符合酵母双杂文库质量要求。利用酵母双杂交系统在该文库中筛选到6个与HVUL0H33228.2相互作用的蛋白,分别为铁氧还蛋白-NADP+氧化还原酶、核酮糖二磷酸羧化酶、叶绿素a/b结合蛋白2和6、光合系统Ⅱ反应中心蛋白CP43和醛缩酶型TIM桶家族蛋白。本研究为进一步揭示HVUL0H33228.2应答白粉菌侵染的分子机制提供了参考依据。 相似文献
2.
番茄叶霉病菌主要生理小种的RAPD标记鉴别 总被引：3，自引：0，他引：3
叶霉病是番茄的主要病害之一,目前我国大部分地区番茄叶霉病菌生理小种以1．2．3,1．2．3．4和1．2．3．4．9为主。本研究旨在利用RAPD技术对3个主要的番茄叶霉病菌生理小种进行分子鉴别。在筛选了41个RAPD随机引物的基础上,利用28个引物对3个生理小种的基因组DNA进行了分析,扩增获得了138个位点,3个生理小种基因组的带型相似率达96．38％.4个引物S89、S40、A30和A31在3个小种间产生了5个多态性位点,可以将3个生理小种完全区分开来。这些RAPD标记可用于番茄叶霉病菌生理小种鉴别,为有效地开展番茄叶霉病菌生理小种分化研究提供了一个新方法。 相似文献
3.
干旱胁迫下斑茅消减文库的构建及分析 总被引：6，自引：0，他引：6
以干旱胁迫下斑茅(Saccharum arundinaceum Retz.)叶片组织的cDNA为Tester，正常生长条件下斑茅叶片组织cDNA为Driver，利用抑制性消减杂交技术（Suppression Subtractive Hybridization, SSH）构建斑茅干旱胁迫消减文库。文库克隆总量约为30 000个，克隆重组率为99.2%；随机从文库中挑取3500个克隆分析表明，插入片断长度主要集中在200~1 000 bp之间，500 bp以上的有463个克隆，500 bp以下的有3 037个克隆，平均长度约450 bp; 通过随机对16个文库阳性克隆的测序及分析，3个克隆与ATP-NAD kinase、Aldo/keto reductase和锌指蛋白高度同源，3个克隆没有同源性，10个与逆境相关，是功能未知的EST。选择编号为EL0149和EL0145两个克隆进行Northern杂交验证，结果表明EL0149克隆为干旱胁迫上调表达的EST序列，而EL0145在正常供水和干旱条件下均无明显的的杂交信号。说明本研究克隆的SSH文库质量较高，可以进一步利用SSH文库结合芯片表达谱分析解析作物水分胁迫下的相关抗旱基因网络。 相似文献
4.
从中蔬四号番茄品种中克隆能在番茄叶片中高效转录的SlU3启动子,为今后利用CRISPR/Cas9基因编辑技术进行分子育种奠定了基础。采用2轮PCR的方法,第1轮PCR从中蔬四号番茄品种中克隆了3种SlU3启动子,再采用Transfer PCR方法分别对3个启动子进行截短,共得到6个不同长度的启动子,并分别构建6个截短的SlU3启动子驱动GUS融合植物表达载体,利用农杆菌转化法分别转染番茄叶片。运用DNAMAN软件对已克隆的SlU3和拟南芥AtU3启动子序列具有转录功能的必要元件进行序列分析;经过2轮PCR,首先从中蔬四号番茄品种中克隆了3种SlU3-1P、SlU3-3P、SlU3-4P启动子,其长度分别是1 156,1 114,1 147 bp,再采用Transfer PCR方法分别对3个启动子进行截短,共得到6个不同长度的启动子,其长度依次是489,318,450,248,457,248 bp,并分别构建6个截短SlU3启动子驱动GUS融合植物表达载体,启动子序列比对分析发现,番茄U3启动子与拟南芥U3启动子一样,也含有比较保守的2个元件,USE和TATA框,2个元件之间的位置比较固定。利用农杆菌转化法分别转染番茄叶片,结果显示,成功侵染后的番茄叶片均被染成蓝色,表明已克隆的6种不同截短番茄SlU3启动子均具有转录活性。成功克隆了6种在番茄叶片中高效转录的SlU3启动子,为构建番茄CRISPR/Cas9基因编辑载体提供更多高效的内源启动子。 相似文献
5.
小麦抗白粉病SSH-cDNA文库中差异基因的表达模式 总被引：2，自引：0，他引：2
为了解白粉菌诱导下抗白粉病小麦的抗病机制,构建了白粉菌接种初期的抑制性消减杂交cDNA(SSH-cDNA)文库。从中随机挑取140个阳性克隆进行测序,去除冗余序列和重复序列后,得到94条EST,利用NCBI的BLAST在线序列比对工具对GenBank的蛋白质数据库进行同源性比对及功能分类。结果表明,49个EST与已知功能蛋白同源性较高,主要涉及初级代谢(2%)、能量代谢(24%)、细胞结构(2%)、转录(2%)、蛋白质合成加工与储藏(16%)、转运(4%)、信号转导(4%)和抗病与防御(30%)。经文库比较,筛选出与病程相关蛋白基因同源的EST(Z25-1)和与谷胱甘肽硫转移酶同源的EST(Z440-1),GenBank登录号为EX567369和EX567360。以其EST序列为依据设计引物,通过RT-PCR分析它们在白粉菌诱导下的表达模式,结果该2基因表达存在明显差异。其中,Z25-1在未接种白粉菌时具有一定的表达量,白粉菌侵染后表达量开始上升,侵染72 h时表达量最高,然后下降;Z440-1在未接种时也具有一定的表达量,但在接种初期表达微弱,甚至不表达,24 h后表达开始上升,到72 h时达最高,然后下降。本研究表明,病程相关蛋白和谷胱甘肽硫转移酶属于诱导型表达基因,且参与白粉病抗病反应,在白粉菌诱导72 h时表达量最高。 相似文献
6.
7.
植物小分子热激蛋白(small heat shock proteins, s HSPs)是一种多样、古老的蛋白家族,分子量大小普遍在12~42 k D之间,包含典型的C端、N端和α晶状体蛋白域。s HSPs作为一种重要的分子伴侣在植物生长发育和响应逆境胁迫中起着重要的作用。本研究通过构建成熟期番茄果实cDNA文库,筛选与SlHSP17.7互作的蛋白,进而探索SlHSP17.7在果实发育进程中的分子机制。以Micro-Tom番茄绿熟期、转色期和完熟期果实cDNA为模板,构建番茄果实c DNA文库。构建pGBKT7-SlHSP17.7诱饵载体筛选与其互作的蛋白,利用酵母双杂交实验验证互作关系。经检测番茄果实cDNA文库库容是2.2×10~6CFU,工作液细胞密度是3.6×10~7cell/mL,均一化程度>106,结果符合质控要求。将pGBKT7-SlHSP17.7诱饵载体转化Y2HGold酵母菌株检测发现,其无毒性和自激活现象,经筛选c DNA文库、测序及序列比对分析得到钙阳离子交换体SlCCX1-like蛋白,利用酵母双杂交实验验证发现二者存在互作关系。经PCR鉴定后,所建文库质量良好,文库的库容和文库滴度均符合建库要求,用pGBKT7-SlHSP17.7诱饵载体筛选得到互作蛋白SlCCX1-like,为进一步研究在果实发育进程SlHSP17.7的生物学功能提供了保障。 相似文献
8.
利迪链霉菌A02诱导番茄抗灰霉病作用机制研究——对植株防御酶系的影响 总被引：3，自引：0，他引：3
利用利迪链霉菌生防菌株A02发酵液和番茄灰霉病菌对番茄植株进行诱导和接种处理,利用分光光度法测定处理前和处理后1~5 d番茄叶片组织的主要防御酶系——苯丙氨酸解氨酶、过氧化物酶、过氧化氢酶和多酚氧化酶的活性变化。试验结果显示,不接种灰霉病菌而单独诱导处理、接种灰霉病菌后诱导处理和诱导处理后接种灰霉病菌这3种处理都能提高番茄植株各防御酶系的活性,后者酶活性峰值最高,对番茄植株灰霉病的抑制作用也最明显,控制效果达94.92%。 相似文献
9.
《华北农学报》2021,36(2)
非生物胁迫环境严重制约了番茄的生产,因此挖掘番茄耐非生物胁迫相关基因尤为重要。前期通过高通量测序筛选获得的UDP-糖基转移酶基因能够参与番茄镉胁迫应答反应,以番茄为试材,进一步研究该基因参与番茄抗逆的功能。首先,利用同源克隆法获得UDP全长的cDNA序列,对该序列进行生物信息学分析,再利用系统进化树分析该蛋白与其他植物的亲缘关系。其次,采用实时荧光定量PCR分析该基因的组织表达特性及其在3种不同非生物胁迫条件下(Cd、PEG-6000、NaCl)的表达模式。进一步利用转基因酵母分析胁迫表型。测序结果显示,该基因的cDNA全长为1 486 bp,包含一个1 452 bp的开放阅读框(ORF),编码483个氨基酸,将其命名为SlUDP。系统进化树分析结果显示该蛋白与马铃薯的亲缘关系最近。实时荧光定量PCR分析结果表明,该基因在番茄不同组织中存在表达差异,在叶中表达量最高,各部位的表达量为叶片果实根部侧茎主茎花。转SlUDP基因酵母胁迫表型分析结果显示,该基因提高了酵母对Cd及干旱的耐受性。根据以上结果推测SlUDP可能在番茄响应重金属镉和干旱胁迫中起到了一定的作用。 相似文献
10.
随着保护地设施的不断发展,无土栽培作为一种高效、节能、无污染的规范化生产模式,已在各地进行推广应用。农十三师农科所利用无土栽培技术种植番茄,单季产量达9 000kg/667m2(旧制1亩,下同),实现了温室番茄的高产、高效。1 品种选择粉色无限生长型番茄奥粉8号,红色无限生长型番茄吉瑞(国外引进)。2 育苗播种时间6月下旬至7月上旬。2.1 苗床及种子处理苗床规格1.5m×5.0m×0.1m砂床,先用清水湿润基质,再用多菌灵或五氯硝基苯1 000倍液将基质淋湿,以地膜覆盖23天并用清水将残留药液清洗干净。 相似文献
11.
Biological Activity and Quantification of Suspected Allelochemicals from Alfalfa Plant Parts 总被引：7，自引：0，他引：7
Autotoxicity restricts reseeding of alfalfa (Medicago sativa L.) after alfalfa until autotoxic chemical(s) breaks down or is dispersed into external environments. A series of aqueous extracts from leaves, stems, roots and seeds of alfalfa ‘Vernal’ were bioassayed against alfalfa seedlings of the same cultivar to determine their autotoxicity. The highest inhibition was found in the extracts from the leaves. Extracts at 40 g dry tissue l?1 from alfalfa leaves were 15.4, 17.5 and 28.7 times more toxic to alfalfa root growth than were those from roots, stems and seeds, respectively. A high‐performance liquid chromatography (HPLC) analysis with nine standard compounds showed that the concentrations and compositions of allelopathic compounds depended on the plant parts. In leaf extracts that showed the most inhibitory effect on root growth, the highest amounts of allelochemicals were detected. Among nine phenolic compounds assayed for their phytotoxicity on root growth of alfalfa, coumarin, trans‐cinnamic acid and o‐coumaric acid at 10?3 m were most inhibitory. The type and amount of causative allelochemicals found in alfalfa plant parts were highly correlated with the results of the bioassay, indicating that the autotoxic effects of alfalfa plant parts significantly differed. 相似文献
12.
C. J. Spurr D. A. Fulton P. H. Brown R. J. Clark 《Journal of Agronomy and Crop Science》2002,188(4):275-280
Development of onion (Allium cepa L., cv. ‘Early Cream Gold’) seed under cool climate conditions in Tasmania, Australia occurred over a longer duration than previously reported, but similar patterns of change in yield components were recorded. In contrast to previous studies, umbel moisture content declined from 85 to 67 % over 57 days while seed moisture content decreased from 85 to 31 %. Seed yield continued to increase over the duration of crop development, with increasing seed weight compensating for seed loss resulting from capsule dehiscence in the later stages of maturation. Germination percentage was high and did not vary significantly from 53 to 77 days after full bloom (DAF), but mean germination time declined and uniformity of germination increased significantly over the same time period. The percentage abnormal seedlings declined with later harvest date, resulting in highest seed quality at 77 DAF. The results of this study suggest that the decision to harvest cool climate onion seed crops before capsule dehiscence will result in a loss of potential seed yield and quality. 相似文献
13.
Jens Jensen 《Euphytica》1979,28(1):47-56
Summary The high-lysine gene in Risø mutant 1508 conditions an increased lysine content in the endosperm via a changed protein composition, a decreased seed size, and several other characters of the seed. The designation lys3a, lys3b, and lys3c, is proposed for the allelic high-lysine genes in three Risø mutants, nos 1508, 18, and 19. Linkage studies with translocations locate the lys3 locus in the centromere region of chromosome 7. A linkage study involving the loci lys3 and ddt (resistance to DDT) together with the marker loci fs (fragile stem), s (short rachilla hairs), and r (smooth awn) show that the order of the five loci on chromosome 7 from the long to the short chromosome arm is r, s, fs, lys3, ddt. The distance from locus r to locus ddt is about 100 centimorgans. 相似文献
14.
G. H. Kroon 《Euphytica》1994,76(1-2):125-125
Summary
K x vadensis is a hybrid of K. blossfeldiana and K. marmorata obtained after doubling the number of chromosomes. 相似文献
15.
Hongyan WEI Jian ZHOU Jinguang LU Jingzheng SONG Qiongxi YU Zhihong JIANG 《Medicinal Plant》2019,(6):27-29
[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenol... 相似文献
16.
Summary Avoidance of rust fungi that was based on poor appressorium induction was previously found in Hordeum chilense. In the present study 95 accessions of Triticeae were screened for avoidance of Puccinia hordei. The percentage of appressorium formation per germinated spore ranged from 6 to 90%. On none of the 41 accessions of Aegilops, Agropyron, Elymus, Secale, Thinopyrum or Triticum studied was the rate of appressorium formation lower than 25%. Lower rates of appressorium formation were, however, found on accessions of wild barley species Hordeum brachyantherum, H. marinum, H. parodii and H. secalinum. Its implications in cereal breeding are discussed. 相似文献
17.
Summary Reactions of 13 grasses to Meloidogyne naasi varied with species; ryegrasses, fescues and their hybrids were generally susceptible and cocksfoot and timothy resistant. Marked variation in host resistance levels occurred between genotypes within cultivars.Selection of single plants, followed by tests on replicate tillers, identified resistant and susceptible genotypes in both Italian and perennial ryegrass cultivars. Resistant plants had few nematode-induced galls and fewer females and eggs than susceptibles. There was more or less continuous variation, with many genotypes intermediate between extremes of resistance and susceptibility. Selected resistant and susceptible genotypes are of use in assessing variation in nematode populations and as controls for breeding and selection programmes. 相似文献
18.
W. Erskine M. Tufail A. Russell M. C. Tyagi M. M. Rahman M. C. Saxena 《Euphytica》1993,73(1-2):127-135
Lentil production is limited by lack of moisture and unfavorable temperatures throughout its distribution. Waterlogging and salinity are only locally important. Progress has been made in breeding for tolerance to drought through selection for an appropriate phenology and increased water use efficiency and in breeding for winter hardiness through selection for cold tolerance.The diseases rust, vascular wilt, and Ascochyta blight, caused by Uromyces viciae-fabae, Fusarium oxysporum f. sp. lentis, and Ascochyta fabae f. sp. lentis, respectively, are the key fungal pathogens of lentil. Cultivars with resistance to rust and Ascochyta blight have been released in several countries and resistant sources to vascular wilt are being exploited. Sources of resistance to several other fungal and viral diseases of regional importance are known. In contrast, although the pea leaf weevil (Sitona spp.) and the parasitic weed broomrape (Orobanche spp.), and to a lesser extent the cyst nematode (Heterodera ciceri), are significant yield reducers of lentil, no sources of resistance to these biotic stresses have been found. Directions for future research in lentil on both biotic and abiotic stresses are discussed. 相似文献
19.
D. A. Bond G. J. Jellis G. G. Rowland J. Le Guen L. D. Robertson S. A. Khalil L. Li-Juan 《Euphytica》1993,73(1-2):151-166
Progress is being made, mainly by ICARDA but also elsewhere, in breeding for resistance to Botrytis, AScochyta, Uromyces, and Orobanche; and some lines have resistance to more than one pathogen. The strategy is to extend multiple resistance but also to seek new and durable forms of resistance. Internationally coordinated programs are needed to maintain the momentum of this work.Tolerance of abiotic stresses leads to types suited to dry or cold environments rather than broad adaptability, but in this cross-pollinated species, the more hybrid vigor expressed by a cultivar, the more it is likely to tolerate various stresses. 相似文献
20.
Chunli TANG Fengxian ZHAO Hongxia CHEN Jiabao MA Jiangcun WEI Meiyan QIU Zhen XIE 《Medicinal Plant》2019,(6):60-65
[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination... 相似文献