共查询到20条相似文献，搜索用时 62 毫秒
1.
海兰鸡IL-18全基因的克隆与序列分析 总被引：1，自引：0，他引：1
IL-18是一种能诱导产生IFN-γ的新型细胞因子,在调节Th1型细胞免疫应答中起重要作用。根据GenBank发表的鸡IL-18cDNA基因序列,自行设计一对引物,经植物血凝素(PHA)活化60日龄海兰鸡的脾淋巴细胞后,提取其总RNA,经反转录-聚合酶链反应(RT-PCR)扩增,扩增产物进行了T-A克隆、测序,获得了中国海兰鸡IL-18基因全序列,其大小为597 bp,与GenBank中查得的鸡IL-18基因进行比较发现,中国海兰鸡IL-18基因与Schneider报道的鸡IL-18基因序列完全一致,为进一步研究鸡IL-18基因表达、生物学活性和应用奠定了基础。 相似文献
2.
海兰鸡白细胞介素-18全基因的克隆与序列分析 总被引：7，自引：1，他引：7
白细胞介素-18（Interleukin-18，IL-18）是一种能诱导产生IFN-γ的新型细胞因子，在调节Th1型细胞免疫应答中起重要作用。根据GenBank发表的鸡IL-18cDNA基因序列，自行设计一对引物，经植物血凝素（PHA）活化60日龄海兰鸡的脾淋巴细胞后，提取其总RNA，经反转录-聚合酶链反应（RT—PCR）扩增，扩增产物进行了T-A克隆、测序，获得了中国海兰鸡IL-18基因全序列，其大小为597bp，与在GenBank中查得的鸡IL-18基因进行比较发现，中国海兰鸡IL-18基因与Schneider报道的鸡IL-18基因序列完全一致，为进一步研究鸡IL-18基因表达、生物学活性和应用奠定了基础。 相似文献
3.
根据国外已发表的鸡白细胞介素 18(IL- 18) c DNA基因序列设计了 1对特异性引物 ,应用 RT- PCR技术 ,从鸡新城疫 系病毒接种 4 8h左右的罗曼鸡胚脾细胞中扩增出鸡 IL - 18全基因 ,并进行了序列测定。结果表明 ,扩增片段全长 5 94 bp,共编码 198个氨基酸的前体蛋白 ,其中含有表达完整功能蛋白所必需的起始密码子和终止密码子。该序列与国外报道的鸡 IL - 18全基因核苷酸序列及推导的氨基酸序列的同源性分别为 99.8%和 10 0 % ;序列中编码成熟蛋白的这段基因与国内报道的源自白来航鸡编码 IL- 18成熟蛋白的基因核苷酸序列及推导的氨基酸序列的同源性分别为 99.8%和 99.4 %。本研究为鸡 IL - 18的扩增及其他细胞因子的扩增提供了一种简便易行的新方法 ,为进一步研究IL- 18基因的结构、功能、表达及表达产物的应用奠定了良好基础 相似文献
4.
编码鸡IL-18成熟蛋白基因的分子克隆与序列测定 总被引：19，自引：1，他引：19
白细胞介素18（IL-18）是一种能诱导IFN-γ产生的新型细胞因子，在调节Th1型细胞免疫应答中起重要作用，本研究根据已发表的鸡白介素18（IL-18）cDNA基因序列设计引物，用反转录-聚合酶链反应（RT-PCR）技术从有丝分裂原ConA刺激48小时活化的白来航鸡脾细胞中扩增出编码鸡IL-18成熟蛋白质的基因。并进行序列测定。并与结果进行了比较。发现本研究克隆到的鸡IL-18成熟蛋白编码基因序列在482位为C，而Schnieder等报道的序列为T，这一核苷酸序列的变化导致了推导的对应氨基酸残基由苯丙氨酸变为丝氨酸。该处碱基变化的原因究竟是鸡 IL-18本身存在多态性，还是反转录或PCR过程中的碱基错配所致，该处碱基的变化对于鸡IL-18蛋白的表达及其生物活性究竟有无影响等问题正在研究中，该基因为国内首次报道经序列测定证实的鸡IL-18基因，为进一步体外表达鸡IL-18蛋白并深入研究其功能奠定了基础。 相似文献
5.
根据GenBank上发表的鸡IL-15基因序列设计两对引物,以从鸡脾脏中提取的基因组RNA为模板,采用RT-PCR扩增出鸡白细胞介素-15基因并进行了序列分析。序列分析结果表明,鸡白细胞介素-15基因序列全长580bp,开放阅读框架内564个核苷酸共编码188个氨基酸。所扩增的序列与已报道的序列同源性为99.9%(563/564)。将扩增序列插入大肠杆菌载体pBV220中进行表达,SDS-PAGE显示目的蛋白约为21ku,表达产物经免疫荧光鉴定为阳性。 相似文献
6.
根据GenBank中鸡白细胞介素18（inteleukin 18,IL-18）和粒细胞-巨噬细胞集落刺激因子（granulocyte-macrophage colony stimulating factor,GM-CSF）基因序列,克隆白来杭鸡IL-18和GM-CSF基因并进行序列分析。结果显示：白来杭鸡IL-18基因开放阅读框为597 bp,编码199个氨基酸,所获得的白来杭鸡IL-18基因与GenBank鸡IL-18的核苷酸同源性为99.0%～99.8%,氨基酸同源性为97.5%～99.5%。白来杭鸡GM-CSF基因开放阅读框为435 bp,编码145个氨基酸,所获得的白来杭鸡GM-CSF基因与GenBank鸡GM-CSF的核苷酸同源性为99.3%～100%,氨基酸同源性为99.3%-100%。IL-18与GM-CSF基因编码的蛋白具有亲水性,都有很强的抗原性。IL-18共有2个潜在的N-糖基化位点,可能存在4个蛋白激酶C磷酸化位点及3个酪蛋白激酶Ⅱ磷酸化位点。GM-CSF共有1个潜在的N-糖基化位点,可能存在1个蛋白激酶C磷酸化位点及4个酪蛋白激酶Ⅱ磷酸化位点。本研究结果为进一步研究鸡IL-18、GM-CSF基因表达、生物学活性和应用奠定了基础。 相似文献
7.
根据GenBank发表的奶牛白细胞介素2(IL-2)基因序列,设计一对特异性引物,应用RT-PCR技术扩增奶牛IL-2基因,琼脂糖电泳显示扩增出的片段约为477bp。回收片段,克隆入pMD18-T载体,经菌落PCR、酶切及重组质粒PCR鉴定,测序结果显示,克隆的奶牛IL-2基因与GenBank发表的奶牛IL-2基因序列同源性为98.7%。该序列与水牛和山羊的IL-2基因序列同源性最大,在95%以上;氨基酸和抗原性比较分析显示,奶牛的IL-2与山羊及水牛的IL-2在这两方面有较高的同源性。 相似文献
8.
鸡白细胞介素18成熟蛋白全长基因的克隆与序列测定 总被引：2，自引：0，他引：2
根据已发表的鸡白细胞介素 18(IL- 18)成熟蛋白的 c DNA序列 ,设计 1对特异性引物 ,应用 RT- PCR从脂多糖(L PS)刺激 10 h活化的 MDCC- MSB1细胞中克隆到编码鸡 IL - 18成熟活性蛋白的完整基因片段 ,其大小为 5 10 bp。经序列测定证实 ,所获得的 c DNA基因序列与文献报道的结果完全一致 ,从而为进一步研究鸡 IL- 18的基因表达、生物学活性以及应用奠定了基础。 相似文献
9.
10.
四川草科鸡IL-15 cDNA的克隆与序列分析 总被引：1，自引：0，他引：1
根据GenBank上登录的鸡白介素15(IL-15)基因序列,设计合成一对特异性引物,以ConA诱导的鸡脾淋巴细胞提取的总RNA为模板进行RT-PCR扩增,并克隆到pMD18-T载体测序.测序结果表明,四川草科鸡IL-15开放阅读框(ORF)由564个核苷酸组成,编码187个氨基酸,与GenBank中公布的鸡白介素15基因进行比较,核苷酸和氨基酸同源性为98.6%～99.5%.生物信息学分析表明,该基因编码的蛋白具有亲水性,有很强的抗原性,共有3个潜在的N-糖基化位点,可能存在3个蛋白激酶C磷酸化位点,存在2个酪蛋白激酶Ⅱ磷酸化位点. 相似文献
11.
Rassnick KM Gieger TL Williams LE Ruslander DM Northrup NC Kristal O Myers NC Moore AS 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2001,15(3):196-199
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks. 相似文献
12.
Davis MS Willard MD Nelson SL Mandsager RE McKiernan BS Mansell JK Lehenbauer TW 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(3):311-314
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large. 相似文献
13.
Furosemide continuous rate infusion in the horse: evaluation of enhanced efficacy and reduced side effects 总被引：1，自引：0，他引：1
Johansson AM Gardner SY Levine JF Papich MG LaFevers DH Fuquay LR Reagan VH Atkins CE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(6):887-895
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses. 相似文献
14.
Waruiru RM Thamsborg SM Nansen P Kyvsgaard NC Bogh HO Munyua WK Gathuma JM 《Tropical animal health and production》2001,33(3):173-187
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round. 相似文献
15.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host. 相似文献
16.
17.
Evaluation of biohydrogenation rate of canola vs. soya bean seeds as unsaturated fatty acids sources for ruminants in situ 
下载免费PDF全文
下载免费PDF全文 S. Pashaei T. Ghoorchi A. Yamchi 《Journal of animal physiology and animal nutrition》2016,100(2):211-216
An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro‐organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed. 相似文献
18.
Serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in Shandan county of Gansu province. The liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. Most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. The liver contained the highest concentrations of copper, manganese and cobalt and the kidney of selenium, iron and calcium.Abbreviations Alb
albumin
- ALT
alanine aminotransferase
- AST
aspartate aminotransferase
- BUN
blood urea nitrogen
- Chol
cholesterol
- Glob
globulin
- -GT
-glutamyl transpeptidase
- IP
inorganic phosphorus
- TP
total protein 相似文献
19.
正We are pleased to publish the second special issue on avian brood parasitism and to be responsible guest editors for the two special issues of Chinese Birds (Vol. 3, No. 4, 2012 and Vol. 4, No. 1, 2013), entitled "Avian Brood Parasitism - A Growing Research Area in Behavioral Ecology". The first issue was published in December 2012. The goal of the two special issues is to publish accumulated knowledge and some of the recent developments in 相似文献
20.
Tropical Animal Health and Production - 相似文献