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1.
两种铜盐与添加水平对蛋鸡血液某些生理生化指标的影响 总被引：4，自引：0，他引：4
选择 2 3周龄海兰褐商品蛋鸡 396只 ,随机分为 11个处理组 ,对照组日粮中不添加铜 ,其余分别添加 6 ,15 ,30 ,6 0 ,12 5mg/kg的硫酸铜或柠檬酸铜。 8周后每组随机取 8只鸡 ,测定其血清总蛋白、总胆固醇、甘油三酯水平和血红蛋白含量 ,以及血清总抗氧化能力、谷胱苷肽S转移酶活力。结果表明当柠檬酸铜添加量为 30 ,6 0 ,12 5mg/kg时 ,其血红蛋白、血浆总蛋白、血清总抗氧化能力和谷胱苷肽S转移酶活力 ,均比对照组显著升高 (P <0 .0 5 ) ,而甘油三酯和总胆固醇含量较对照组显著降低 (P <0 .0 5 )。结果显示 ,柠檬酸铜的添加效果要好于硫酸铜。 相似文献
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日粮不同铜、钼水平对辽宁绒山羊机体抗氧化性能的影响 总被引：1，自引：0，他引：1
试验选用性别一致、年龄、体重相近的36只健康辽宁绒山羊,研究日粮中添加不同水平的铜(01、9mg/kgDM和38 mg/kg DM)和钼(0、5 mg/kg DM)对绒山羊机体抗氧化性能的影响。结果表明:日粮添加铜可显著增强绒山羊机体抗氧化性能,显著提高血铜浓度、铜蓝蛋白、铜-锌-SOD及谷胱甘肽过氧化物酶活性(P0.01),显著降低丙二醛含量(P0.01),但添加铜19 mg/kg DM和38 mg/kg DM差异不显著(P0.05)。日粮添加钼5 mg/kgDM显著提高谷胱甘肽过氧化物酶活性(P0.01),对其他指标无影响(P0.05)。铜钼对上述指标无显著互作效应(P0.05),但在铜蓝蛋白、铜-锌-SOD和谷胱甘肽过氧化物酶上存在互作趋势。 相似文献
3.
本试验旨在研究饲粮不同铜源及水平对育成期水貂血清铜、锌和铁含量及血清氮代谢、脂类和抗氧化指标影响,以及水貂对不同铜源的相对生物学利用率。选择60日龄健康、体重接近的水貂240只,随机分成10组,每组24个重复,每个重复1只貂。试验采用3×3+1试验设计,对照组饲喂基础饲粮,试验组分别在基础饲粮中添加3种铜源(硫酸铜、碱式氯化铜、蛋氨酸铜)和3个铜添加水平(10、25、40 mg/kg),预试期7 d,正试期45 d。结果表明：水貂血清铜含量随饲粮铜添加水平增加呈极显著线性增加( P=0.0004)。水貂血清总胆固醇( P=0.0015)、甘油三酯( P=0.0021)和高密度脂蛋白胆固醇( P=0.0101)含量随饲粮铜添加水平增加呈显著或极显著线性降低,然而水貂血清低密度脂蛋白胆固醇含量却未受饲粮铜添加水平的影响(P=0.5790)。水貂血清铜蓝蛋白和铜锌超氧化物歧化酶活性均随饲粮铜添加水平增加呈极显著线性升高( P=0.0001)。以血清铜蓝蛋白活性为指标,以硫酸铜为参比标准物(100%),碱式氯化铜和蛋氨酸铜的相对生物学利用率分别为109.93%和131.06%。以血清铜锌超氧化物歧化酶活性为指标,以硫酸铜为参比标准物(100%),碱式氯化铜和蛋氨酸铜的相对生物学利用率分别为111.01%和118.10%。由此可见,饲粮添加40 mg/kg的铜(基础饲粮铜含量8.05 mg/kg)能提高育成期水貂血清抗氧化酶的活性;育成期水貂3组铜源的相对生物学利用率为：蛋氨酸螯合铜>碱式氯化铜>硫酸铜。 相似文献
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《中国畜牧杂志》2015,(21)
选用75头遗传背景相同、胎次相近、公母各半,约45日龄,体重(14.00±0.30)kg的三元杂交健康仔猪,采用完全随机化设计随机分为5个处理组,每个处理组5个重复,每个重复3头。研究不同添加水平(10、150、300 mg/kg)和不同来源的(硫酸铜,蛋氨酸铜)铜对猪部分血液生化指标及含铜酶活性的影响。结果表明:添加不同铜源和水平的铜对猪日增重和饲料增重比均无显著差异(P0.05);添加不同铜源对甘油三酯、谷草转氨酶、谷丙转氨酶含量、谷胱甘肽过氧化物酶和铜锌超氧化物歧化酶活性无显著影响(P0.05);300 mg/kg铜可显著提高谷丙转氨酶A活性(P0.01)、血清铜兰蛋白活性(P0.01);显著降低血清胆固醇、血清尿素氮含量(P0.01)。添加150 mg/kg铜均显著提高血清谷胱甘肽过氧化物酶和铜锌超氧化物歧化酶活性(P0.05)。氨基酸铜源可显著提高血清铜兰蛋白活性,显著降低血清胆固醇、血清尿素氮含量(P0.01)。 相似文献
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《动物营养学报》2021,33(7)
本试验旨在研究饲粮中添加柠檬酸铜对肉兔生长性能、小肠黏膜发育和血清生化指标的影响。选择28日龄同期断奶新西兰肉兔420只,随机分为5组,每组14个重复,每个重复6只(公母各占1/2),分别饲喂在基础饲粮中添加0(对照组)、10、20 mg/kg(以铜计)柠檬酸铜以及20、150 mg/kg(以铜计)硫酸铜的试验饲粮,各组饲粮铜含量实测值依次为5.1、15.6、25.9、25.2和153.4 mg/kg。预试期7 d,正试期42 d。结果显示:1)与对照组相比,饲粮中添加20 mg/kg柠檬酸铜可以显著提高肉兔的平均日增重(P0.05),显著降低料重比、死亡率和腹泻率(P0.05),且20 mg/kg柠檬酸铜组与150 mg/kg硫酸铜组之间上述指标无显著差异(P0.05)。2)与对照组相比,饲粮中添加20 mg/kg柠檬酸铜可以显著提高肉兔小肠各段绒毛高度和绒毛高度/隐窝深度(P0.05),且20 mg/kg柠檬酸铜组与150 mg/kg硫酸铜组之间上述指标无显著差异(P0.05);小肠各段隐窝深度各组之间差异不显著(P0.05)。3)与对照组相比,饲粮添加20 mg/kg柠檬酸铜可以显著提高肉兔血清中铜锌超氧化物歧化酶、铜蓝蛋白活性和胰岛素、生长激素含量(P0.05),其中20 mg/kg柠檬酸铜组与150 mg/kg硫酸铜组在血清铜锌超氧化物歧化酶、铜蓝蛋白活性和生长激素含量上差异不显著(P0.05)。综上所述,饲粮中添加20 mg/kg柠檬酸铜可以促进肉兔生长,降低腹泻率和死亡率,促进肠道发育,改善血清生化指标,其作用效果与150 mg/kg硫酸铜相当。 相似文献
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日粮中添加高水平铜对雏鸭血液指标的影响 总被引：1，自引：0，他引：1
试验对日粮中添加高水平铜对雏鸭血液指标的影响进行了研究。360只1日龄天府肉鸭随机分为6组，分别喂以对照日粮（Cu 8mg／kg）和高铜日粮（Cu100mg／kg，高铜Ⅰ组；Cu200mg／kg，高铜Ⅱ组；Cu400mg／kg，高铜Ⅲ组，Cu600 mg／kg，高铜Ⅳ组；Cu800mg／kg，高铜V组）6周。与对照组比较，高铜Ⅳ组和V组的红细胞数量、血红蛋白含量、血清铜蓝蛋白活性和血清铜锌SOD活性降低（P〈0．0）1），高铜Ⅲ组、Ⅳ组和V组血清丙氨酸氨基转移酶、天门冬氨酸氨基转移酶活性显著升高（P〈0．01），胆固醇、甘油三酯和总胆红素含量显著增加（P〈0．01）。结果表明．日粮铜水平为400mg／kg及以上时可引起上述血液指标的改变。 相似文献
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试验旨在研究不同铜源对育成期乌苏里貉生长性能、血清生化指标的影响。随机选取(60±5)日龄健康的雄性乌苏里貉60只,随机分为4组,每组15个重复,每个重复1只。分别添加硫酸铜(Ⅰ组)、碱式氯化铜(Ⅱ组)、混合铜(Ⅲ组,碱式氯化铜和蛋氨酸铜,添加比例为11,以铜元素计)和蛋氨酸铜(Ⅳ组),添加水平为40 mg/kg的试验饲粮,预饲期7 d,正式期62 d。试验结束后,每只动物后肢静脉采血。结果表明:①不同铜源对不同阶段乌苏里貉体重和日增重没有显著差异(P>0.05)。②不同铜源对血清丙氨酸氨基转移酶(ALT)、天门冬酸氨基转移酶(AST)、血糖(GLU)、铜锌超氧化物歧化酶(Cu-Zn SOD)、铜蓝蛋白(CP)含量没有显著差异(P>0.05),Ⅰ组血清中乳酸脱氢酶(LDH)显著高于Ⅳ组(P<0.05),Ⅲ、Ⅳ组血清中碱性磷酸酶(ALP)含量显著高于Ⅰ组(P<0.05)。Ⅲ组血清中总超氧化物(T-SOD)含量显著高于Ⅳ组,Ⅳ组血清中T-SOD含量显著高于Ⅰ、Ⅱ组(P<0.05)。在本试验条件下,添加有机铜的组别可以提升ALP与T-SOD含量,添加硫酸铜组更容易危害动物健康。因此在实践生产中,为提高生长性能和降低饲养成本,建议有机铜与无机铜混合使用。 相似文献
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试验旨在研究饲粮中添加白藜芦醇(resveratrol,RES)对"杜×长×大"猪空肠抗氧化酶活性及抗氧化基因指标和紧密连接蛋白相关基因表达水平的影响。试验选用12头体重约为65 kg的育肥猪分为2组,每组6头。对照组育肥猪饲喂基础饲粮,试验组育肥猪饲喂在基础饲粮中添加400 mg/kg的RES的饲粮,试验期41 d。结果表明,与对照组相比,饲粮中添加400 mg/kg的RES显著提高了育肥猪空肠过氧化氢酶(CAT)、总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性及总抗氧化能力(T-AOC)(P<0.05),显著降低丙二醛(MDA)的含量(P<0.05);显著提高育肥猪空肠抗氧化基因铜/锌超氧化物歧化酶(Cu/Zn-SOD)、锰超氧化物歧化酶(Mn-SOD)、谷胱甘肽过氧化物酶-1 (GPx-1)和谷胱甘肽过氧化物酶-4 (GPx-4)活性(P<0.05);显著提高育肥猪空肠抗氧化酶活相关基因CAT、T-SOD和GSH-Px的表达水平(P<0.05),显著降低MDA的表达水平(P<0.05);显著提高育肥猪空肠紧密连接蛋白咬合蛋白(Occludin)、闭合蛋白-1 (Claudin-1)和链接黏附分子A (JAM-A)的表达水平(P<0.05)。研究表明,饲粮添加400 mg/kg的RES能够显著提高育肥猪空肠抗氧化能力及紧密连接蛋白的表达,改善动物肠道健康。 相似文献
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饲粮添加单月桂酸甘油酯对蛋鸡生产性能、蛋品质、血清生化指标、免疫器官指数和腹脂形态的影响 总被引：1，自引：0，他引：1
《中国家禽》2017,(17)
为研究饲粮添加单月桂酸甘油酯(GML)对蛋鸡生产性能、蛋品质、血清生化指标、免疫器官指数和腹脂形态的影响,以44周龄海兰褐蛋鸡为研究对象,在饲粮中添加150、300和450 mg/kg GML饲喂8周后分析蛋鸡相关指标的变化。结果表明,与对照组相比,饲粮添加300和450 mg/kg GML可以提高蛋鸡产蛋率、血清中超氧化物歧化酶和谷胱甘肽过氧化物酶的活力(P0.05),同时显著降低血清中丙氨酸氨基转移酶的活力,丙二醛、总胆固醇、低密度脂蛋白胆固醇和总胆红素的含量以及腹脂率(P0.05)。饲粮添加300 mg/kg GML可以显著提高鸡蛋蛋白高度、哈氏单位和蛋黄颜色(P0.05)。饲粮添加450 mg/kg GML还可以显著提高血清中总蛋白和高密度脂蛋白胆固醇的含量(P0.05),同时显著降低蛋鸡血清中天门冬氨酸氨基转移酶活力(P0.05)。此外,饲粮添加GML可以显著降低蛋鸡料蛋比和血清中甘油三酯的含量以及脂肪细胞的平均面积、平均周长和平均体积(P0.05),显著提高蛋鸡单个蛋重、脾脏指数和胸腺指数(P0.05)。总体来看,饲喂GML对提高蛋鸡生产性能、蛋品质、抗氧化能力和免疫功能,改善蛋鸡脂肪代谢和健康状况具有积极的作用。 相似文献
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高铜对雏鸭抗氧化酶活性的影响 总被引：1，自引：1，他引：1
360只1日龄天府肉鸭随机分为6组,分别喂以对照日粮(Cu 8 mg/kg)和高铜日粮(Ⅰ组Cu 100 mg/kg;Ⅱ组Cu 200 mg/kg;Ⅲ组Cu 400 mg/kg;Ⅳ组Cu 600 mg/kg;Ⅴ组Cu 800 mg/kg),试验期6周.与对照组比较,高铜Ⅳ组和Ⅴ组血清铜锌SOD、CP活性、肝脏铜锌SOD、GSH-Px活性降低(P<0.01);高铜Ⅲ组、Ⅳ、Ⅴ组肝脏黄嘌呤氧化酶、单胺氧化酶含量显著升高(P<0.01);高铜Ⅰ、Ⅱ组血清铜锌SOD、CP活性、肝脏铜锌SOD、GSH-Px活性升高(P<0.01).结果表明,日粮铜水平达400 mg/kg及以上时可引起血清和肝脏抗氧化酶活性降低,使机体抗氧化功能受损. 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献
19.
乳酸杆菌作为益生菌广泛用于人和动物.本文综述了乳酸杆菌改善宿主健康的机制.乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道.文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制. 相似文献
20.
Billinghurst RC Brama PA van Weeren PR Knowlton MS McIlwraith CW 《American journal of veterinary research》2004,65(2):143-150
OBJECTIVE: To determine whether serum concentrations of biomarkers of skeletal metabolism can, in conjunction with radiographic evaluation, indicate severity of osteochondrosis in developing horses. ANIMALS: 43 Dutch Warmblood foals with varying severity of osteochondrosis. PROCEDURE: 24 foals were monitored for 5 months and 19 foals were monitored for 11 months. Monthly radiographs of femoropatellar-femorotibial and tibio-tarsal joints were graded for osteochondral abnormalities. Serial blood samples were assayed for 8 cartilage and bone biomarkers. At the end of the monitoring period, foals were examined for macroscopic osteochondrosis lesions. RESULTS: Temporal relationships were evident between certain serum biomarkers and osteochondrosis severity in foals during their first year. Biomarkers of collagen degradation (collagenase-generated neoepitopes of type-II collagen fragments, type-I and -II collagen fragments [COL2-3/4C(short)], and cross-linked telopeptide fragments of type-I collagen) and bone mineralization (osteocalcin) were positive indicators of osteochondrosis severity at 5 months of age. In foals with lesions at 11 months of age, osteochondrosis severity correlated negatively with COL2-3/4C(short) and osteocalcin and positively with C-propeptide of type-II procollagen (CPII), a collagen synthesis marker. Radiographic grading of osteochondrosis lesions significantly correlated with macroscopic osteochondrosis severity score at both ages and was strongest when combined with osteocalcin at 5 months and CPII at 11 months. CONCLUSIONS AND CLINICAL RELEVANCE: The ability of serum biomarkers to indicate osteochondrosis severity appears to depend on stage of disease and is strengthened with radiography. In older foals with more permanent lesions, osteochondrosis severity is significantly related to biomarker concentrations of decreased bone formation and increased cartilage synthesis. 相似文献