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1.
多种动物血清中生物活性LH/CG的放射受体测定法(RRA)   总被引：1，自引：0，他引：1  
利用绵羊卵巢黄体细胞膜作为 LH / CG受体来源 ,以放射性12 5 I- h CG作为示踪标记配体 ,以高纯度的h CG为标准品建立标准曲线 ,建立了一种有效的 LH- RRA测定方法。它能够直接检测多种动物血清中与受体结合的具有生物活性的 LH的动态变化。该法测定程序简单 ,在 2 h内完成并获得可重复性结果。测定方法的灵敏度为 0 .3ng/管 ,批内和批间误差分别为 ( 3.4 1± 3.14 ) %和 ( 8.70± 7.93) %。动物 (牛、兔、猪 )垂体液中 LH含量反应曲线和 h CG标准曲线平行良好。牛、羊、猪、兔、鼠和仓鼠血清中 LH的剂量反应曲线和h CG标准曲线的平行性表明 ,该 LH- RRA可以用来检测不同动物血清中的具有生物活性的 LH变化。回收试验表明 ,在该测定方法中加入 3、10、30 ng h CG时的回收率分别为 ( 10 0 .59± 5.55) %、( 99.55± 9.86 ) %、( 10 4 .2 8± 7.4 5) %,说明测定结果的正确性。该 LH - RRA的测定结果反映了动物正常的生理状态。本试验垂体中有活性的 LH水平远远高于外周循环中的水平 ,雌、雄动物循环血液中的 LH含量不一致。  相似文献   

2.
文章采用体外细胞培养和放射免疫测定(RIA)等方法,研究了褪黑素(MLT)对季节性繁殖的蒙古母羊垂体细胞分泌促卵泡素(FSH)、促黄体素(LH)和促乳素(PRL)的作用。结果表明:当单独用递增的MLT(0、10、100、1000pg/ml)处理原代垂体细胞时,伴随时间的延长FSH的分泌量极显著下降(P<0·01),但对LH的基础分泌没有影响;用10IU/ml hCG单独刺激或用不同剂量MLT与10IU/ml hCG共同刺激垂体细胞,FSH和LH的分泌都极显著高于对照组(P<0·01),但与MLT的剂量没有关系。MLT能改变抑制垂体细胞PRL的分泌,用10IU/ml HCG单独作用使垂体细胞PRL分泌减少,但这种作用可被MLT呈剂量依赖性抑制。本研究表明MLT可作用于季节性繁殖蒙古绵羊的垂体细胞并影响其促性腺激素的分泌。  相似文献   

3.
《畜牧与兽医》2013,(11):66-68
为了探讨肌肉生长抑制素(MSTN)蛋白主动免疫对雌兔生殖激素合成与分泌的作用,本试验选择30只2月龄的雌兔随机分为3组,分别用生理盐水(对照组)、0.26 mL MSTN蛋白(试验1组)、0.52 mL MSTN蛋白(试验2组)进行免疫,每隔15 d以原剂量再免疫1次,共免疫4次。以第1次免疫当天为0 d,分别在0 d、15 d、30 d、45 d、60 d采集血样,采用酶联免疫吸附法分析血样中卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)的含量。结果表明:MSTN蛋白免疫后,试验2组血清中FSH、LH与对照组差异显著(P<0.05);试验1组血清中FSH、LH与对照组差异不显著(P>0.05);各组的血清中E2无显著差异(P>0.05)。研究表明,MSTN蛋白在雌性动物生殖中具有调节作用。  相似文献   

4.
牛促黄体素（bLH）同源放射免疫分析法的建立   总被引：2，自引：2，他引：0  
本实验用兔抗牛LH血清、~(125)I-标记的牛LH建立牛LH的放射免疫分析法。本方法灵敏度为0.12ng USDA-bLH-B-5/ml,与NIH-bTSH-S_(10),USDA-bFSH-BP_3和oPRL的交叉反应分别小于1.0％,0.1％和0。牛血浆添加USDA-bLH-B-5的回收率为100.8±4.7％,标准曲线的范围为0.019-1.25ng USDA-bLH-B-5。组内变异系数为3.4％(n=20)、组间变异系数为5.2％(n=7)。垂体兴奋试验表明本方法能灵敏、特异地测定出牛血液中LH的变化。  相似文献   

5.
LRH—A3对全奶母牦牛的诱导发情效果和作用机理初探   总被引：5，自引：2，他引：3  
《中国畜牧杂志》2002,38(1):14-15
本研究初步探讨了促性腺素释放激素类似物(LRH-A3)对全奶母牦牛(在当年4～6月份产犊的泌乳母牦牛)的诱导发情排卵效果及机理.1998年7月对试验组(n=20)母牦牛注射LRH-A3(300μg/头),对照组(n=42)未作任何处理.9月15日前用普通牛公牛配种,9月15日后用公牦牛补配.1999年产犊率为试验组50.0%(10/20),对照组28.6%(20/42),试验组和对照组差异极显著(P<0.01).对5头母牦牛在注射LRH-A3前30min及注射后30,45和60min血清中促黄体素(LH)浓度测定以进一步探明全奶母牦牛诱导发情第一情期受胎率低的原因.结果发现,虽然注射LRH-A3后60min全奶母牦牛血清中LH浓度显著升高(P<0.05),但该峰值(3.34±0.67ng/ml,n=5)未达到自然发情排卵的LH峰值(17.1829±2.1174ng/ml).  相似文献   

6.
褪黑素对体外培养绵羊垂体细胞分泌FSH和LH的影响   总被引：4，自引：1，他引：3  
本文采用体外细胞培养和放射免疫测定法(RIA)的方法,研究了褪黑素(MLT)对季节性繁殖的蒙古母羊垂体细胞分泌促卵泡素(FSH)和促黄体素(LH)的作用.结果表明当单独用递增的MLT(10、100、1000、2000 pg/mL)处理原代垂体细胞时,随时间的延长FSH的分泌量极显著下降(P<0.01),但对LH的基础分泌没有影响;无论用10IU/mLhCG单独刺激,还是用不同剂量MLT与10IU/mLhCG共同刺激垂体细胞,FSH和LH的分泌都极显著高于对照组(P<0.01),但与MLT的剂量没有关系.  相似文献   

7.
NO是一种性质活泼作用广泛的生物信息分子 ,参与了神经 内分泌 免疫的多种调控。本试验对体成熟SD大鼠灌喂不同剂量的大豆黄酮 ,研究植物雌激素大豆黄酮对大鼠血清、下丘脑、垂体NO分泌的影响。结果表明 ,灌喂不同剂量的大豆黄酮 (1 0 0 ,50 ,0ng/kg)对雄性大鼠血清、下丘脑、垂体NO分泌的影响作用不显著 ;各处理组中NO的血清含量极显著高于下丘脑、垂体的分泌量  相似文献   

8.
采用体外细胞培养和放射免疫测定法（RIA）测定日粮能量对鸟鸡外周血清促性腺激素与垂体分泌促性腺激素的影响。日粮能量处理：低能Ⅱ组、低能I组、对照组、高能I组、高能Ⅱ组;体外培养垂体细胞组分：高能组、对照纽、低能组。结果显示,血清中FSH含量,对照组与高能Ⅱ组相比差异显著（P〈0．05）,与低能I、Ⅱ组相比差异极显著（P〈0．01）;血清中LH含量,对照组与高能I组、高能Ⅱ组组间差异显著（P〈0．05）;低能Ⅱ组与高能I组、低能Ⅱ组与高能Ⅱ组、低能I组与高能I组、低能I组与高能Ⅱ组组间差异极显著（P〈0．01）;单层垂体细胞中FSH含量,高能组和对照组与低能组比差异显著（P〈0．05）;单层垂体细胞中LH含量,高能组与低能组比较差异极显著（P〈0．01）,对照组与低能组比较差异不显著。结果表明,日粮能量对处理体外培养乌鸡垂体细胞分泌FSH、LH有促进作用。  相似文献   

9.
利用阴离子交换柱QHyperD对双峰驼精清进行分离 ,大鼠垂体组织培养及母驼肌注活性组分 ,RIA法测定垂体组织培养液和母驼血浆中LH及FSH的浓度。实验结果表明 ,驼精清经QHyperD柱层析后 ,共得到 6个组分 (F1～F6 ) ,其中F3和F5在大鼠垂体组织培养实验中具有生物活性 ,可引起LH的明显释放 (P <0 .0 5 )。在体实验时给卵巢上有成熟卵泡的母驼分别肌注F3或F5 ,肌注后 5～ 7h ,LH的释放也明显增加 (P <0 .0 5 )。F3可引起母驼发生排卵 ,F5则不能 ,说明F3可能就是驼精清中的诱导排卵活性因子或其成分之一。另外 ,无论是F3或是F5 ,在体内外实验中均未能引起FSH的分泌发生显著变化  相似文献   

10.
本实验对牛垂体混合细胞进行24小时体外无血清培养,对其分泌催乳素的功能进行了观察,并分别在培养介质中加入0、1、10、100ng/ml的孕酮、脱氢表雄酮、雄烯二酮、雌二醇和睾酮,用放射免疫测定法测定培养液介质中催乳素含量,以研究这些性腺激素和中间产物对垂体细胞分泌催乳素的影响。结果表明:孕酮对离体牛垂体细胞无血清培养条件下分泌催乳素的功能没有影响。而脱氢表雄酮、雄烯二酮、睾酮和雌激素均引起催乳素分泌量显著升高,说明这些性腺类固醇激素对牛垂体细胞离体培养分泌催乳素具有促进作用,可能是垂体前叶催乳素分泌调节的重要组成部分。  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
Tropical Animal Health and Production -  相似文献   

14.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

15.
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.  相似文献   

16.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

17.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

18.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

19.
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors.  相似文献   

20.
<正>1.INTRODUCTION Ensuring transportation network security is one Of the most daunting challenges confronting homeland security agencies today.Significant research has been dedicated.To model and analyze the vulnerability of transportation systems,while notably fewer studies propose specific strategies for deploying defensive technologies to safeguard these systems.  相似文献   

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