共查询到20条相似文献，搜索用时 62 毫秒
1.
新疆昌吉部分地区马梨形虫病检测初报 总被引：1，自引：0，他引：1
《中国动物传染病学报》2016,(1)
为了解昌吉养殖马区域马匹感染梨形虫病情况,本文采用聚合酶链反应试验(polymerase chain reaction,PCR)与血液涂片镜检法相结合,对昌吉马匹随机抽样(n=78),进行马梨形虫病感染情况调查。血液涂片结果显示,马泰勒虫阳性率6.4%,驽巴贝斯虫阳性率为2.5%;PCR结果显示,马泰勒感染率为28.2%(22/78),驽巴贝斯虫感染率为10.2%(8/78),混合感染率为10.2%(8/78)。通过对采自马的蜱进行了形态学鉴定,共鉴定出4种蜱。该次对昌吉部分地区马梨形虫感染情况的调查结果,为该地区马的健康发展提供了科学依据。 相似文献
2.
为了解新疆南北疆部分疫区马匹携带驽巴贝斯虫(Babesia caballi)抗体情况,运用实验室已构建保存的地方虫株重组Bc48为靶抗原,将重组原核表达质粒pGEX4T-1-Bc48转化至BL21表达系统中,诱导表达出36 KDa的重组蛋白。经KCl法切胶纯化重组蛋白后作为rELISA包被抗原,对阿克苏、伊犁、和硕等7个区域的随机采集样品(n=256)进行马驽巴贝斯虫抗体检测。结果显示,Bc48重组抗原能够精确识别马驽巴贝斯虫标准阳性和阴性血清;所检测的临床样品中,驽巴贝斯虫抗体阳性率为19.5%,其中,南疆2个地区(和硕、阿克苏)马驽巴贝斯虫病阳性率分别为30%、9%;北疆5个地区(昭苏种马场、伊犁卡拉苏、洪纳海乡、阿尕什敖包乡和富蕴县区域)马驽巴贝斯虫抗体阳性率分别为17%、12.5%、17%、5%和35%。本次调查结果为新疆驽巴贝斯虫防控措施及疫苗和驱虫药的使用提供必要的理论依据。 相似文献
3.
《中国兽医杂志》2019,(7)
为了解新疆部分地区马匹感染马驽巴贝斯虫病的流行情况,通过PCR方法对不同地区所采集的样品进行检测,并对不同地区和不同年龄段马驽巴贝斯虫感染情况进行单因素方差分析。结果显示,所检测的504份马血样中,54份血样为阳性,阳性率为10.71%。其中北疆昭苏种马场、阿勒泰、吐鲁番托克逊马驽巴贝斯虫阳性率分别为20.61%、5.17%、0;巴音布鲁克牧场、和静地区马驽巴贝斯虫阳性率分别为0和8.59%。从阳性样品中都检测到了驽巴贝斯虫BC48基因片段,与已知序列的同源性为99%。X≤3,3X≤6,6X≤9,X9四个年龄段马驽巴贝斯虫感染率分别为9.76%、10.53%、11.21%和11.65%。单因素方差分析结果表明,昭苏种马场与阿勒泰、巴音布克牧场分别存在极显著性差异(P0.01);巴音布鲁克牧场与和静也存在极显著性差异(P0.01);昭苏种马场与吐鲁番托克逊存在显著性差异(P0.05),其余各地方差异不显著。各个年龄段马驽巴贝斯虫感染无显著性差异。本试验的研究数据可为研究新疆马驽巴贝斯虫分布特点提供数据支撑。 相似文献
4.
为了解云南地区马驴骡感染马泰勒虫和驽巴贝斯虫情况,采用双抗原夹心ELISA方法对云南8个地区马、驴、骡三种动物（n=969）进行了马泰勒虫和驽巴贝斯虫抗体阳性率的检测。结果显示：梨形虫总抗体阳性率为5.47%,其中马泰勒虫抗体阳性率为4.75%,驽巴贝斯虫抗体阳性率为4.85%,两者混合感染的抗体阳性率为4.13%;通过调查分析发现云南马、驴、骡存在梨形虫感染,且不同地区的不同动物的马梨形虫感染程度不同。本研究结果为马驴骡梨形虫病的流行防控提供了数据参考。 相似文献
5.
《畜牧与兽医》2016,(1):111-113
马梨形虫病按病程可分为急性、亚急性及慢性,尤其是慢性病例长期携带病原-带虫状态。为了解阿勒泰富蕴县放牧马携带马驽巴贝斯虫和马泰勒虫状况,采用血清学-竞争ELISA方法 (以血液涂片为辅),对放牧马(n=233)进行了马泰勒虫和驽巴贝斯虫2种虫血液循环抗体的检测试验。结果表明:富蕴县放牧马泰勒虫抗体阳性率为13.30%,驽巴贝斯虫抗体阳性率为17.58%,两者混合感染的抗体阳性率为2.15%,说明该县放牧马感染不同程度的马梨形虫病,且出现同一个马场和在同一匹马同时存在2种虫体抗体的情况;在试验抽样病例中几乎未发现有马梨形虫病临床症状,均呈隐性感染状态。因此带虫的放牧马随时有被蜱虫叮咬散播病原的危险,要加强防范。 相似文献
6.
马梨形虫病是由马驽巴贝斯虫和马泰勒虫寄生于马属动物的红细胞内所引起的一类血液原虫病,呈全球性分布,尤其在新疆发病率更高,处于逐年上升趋势,对区域性马产业的发展影响极大。为了解2018年新疆昭苏养马区域马梨形虫的感染情况,随机采集昭苏县18个乡镇的马全血及血清各858份,采用PCR和间接ELISA分别进行检测,对两种方法检测的18个地区、不同年龄阶段的马驽巴贝斯虫、马泰勒虫及混合感染情况进行统计学分析。结果显示,PCR检测马驽巴贝斯虫、马泰勒虫及混合感染的阳性率分别为12.12%、13.87%和2.80%;间接ELISA检测马驽巴贝斯虫、马泰勒虫及混合感染的抗体阳性率分别为15.50%、10.14%和2.56%;不同年龄阶段筛查结果显示,在6岁以下的马匹感染马驽巴贝斯虫、马泰勒虫及混合感染的阳性率较高,并且不同地区的不同年龄阶段马匹的马梨形虫感染率存在不同程度的差异。此次获得的昭苏县马梨形虫感染情况的一线数据,可为当地养马区域马梨形虫病的综合防控提供技术支撑。 相似文献
7.
广州及其周边地区马梨形虫病血清流行病学调查 总被引：2，自引：0，他引：2
为广东从化无规定马属动物疫病区建设的顺利进行和了解周边地区马属动物马梨形虫病的存在情况,采用酶联免疫吸附试验(ELISA)和涂片镜检的方法对广州、东莞和深圳的马属动物进行了马梨形虫病(马巴贝斯虫和驽巴贝斯虫两种虫体)的流行病学调查。结果表明,上述三个地区的马属动物均存在不同程度的马梨形虫病两种虫体的抗体,且出现同一个马场和在同一匹马同时存在两种虫体抗体的情况。对所有抗体阳性马匹进行涂片镜检未发现虫体,也未发现有马梨形虫病临床症状的马属动物。 相似文献
8.
本研究旨在建立马驽巴贝斯虫抗体的快速、准确检测方法。以纯化的马驽巴贝斯虫Bc48基因片段的原核表达产物免疫6周龄雌性BALB/c小鼠制备单克隆抗体,并利用重组抗原与单克隆抗体建立间接竞争ELISA(CI-ELISA)方法检测马驽巴贝斯虫抗体。结果显示:制备出3株能稳定分泌单克隆抗体的杂交瘤细胞株,命名为1H2、7F4、11F4,通过对CI-ELISA条件筛选得出,抗原最佳包被浓度为0.19μg·mL~(-1),单克隆抗体11F4的最佳工作浓度为1∶3.2×10~5,通过检测30份马驽巴贝斯虫阴性血清及20份阳性血清,确定该检测方法临界值为45%;特异性试验发现,该CI-ELISA方法不与感染马泰勒虫病的阳性血清发生反应,具有特异性;用所建立的CI-ELISA检测临床血清90份,与标准c-ELISA试剂盒总符合率为92.2%、阳性符合率92.1%、阴性符合率94.1%。试验结果表明,该CI-ELISA方法特异性强,敏感性高,稳定性和重复性好,操作简便。基于单克隆抗体(11F4)与重组蛋白(HIS-Bc48)所建立的CI-ELISA特异性、重复性好,可为新疆马驽巴贝斯虫的检测、监控提供有效手段。 相似文献
9.
10.
11.
12.
魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
13.
14.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
15.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
16.
17.
18.
Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献
19.
乳酸杆菌作为益生菌广泛用于人和动物.本文综述了乳酸杆菌改善宿主健康的机制.乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道.文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制. 相似文献
20.
Billinghurst RC Brama PA van Weeren PR Knowlton MS McIlwraith CW 《American journal of veterinary research》2004,65(2):143-150
OBJECTIVE: To determine whether serum concentrations of biomarkers of skeletal metabolism can, in conjunction with radiographic evaluation, indicate severity of osteochondrosis in developing horses. ANIMALS: 43 Dutch Warmblood foals with varying severity of osteochondrosis. PROCEDURE: 24 foals were monitored for 5 months and 19 foals were monitored for 11 months. Monthly radiographs of femoropatellar-femorotibial and tibio-tarsal joints were graded for osteochondral abnormalities. Serial blood samples were assayed for 8 cartilage and bone biomarkers. At the end of the monitoring period, foals were examined for macroscopic osteochondrosis lesions. RESULTS: Temporal relationships were evident between certain serum biomarkers and osteochondrosis severity in foals during their first year. Biomarkers of collagen degradation (collagenase-generated neoepitopes of type-II collagen fragments, type-I and -II collagen fragments [COL2-3/4C(short)], and cross-linked telopeptide fragments of type-I collagen) and bone mineralization (osteocalcin) were positive indicators of osteochondrosis severity at 5 months of age. In foals with lesions at 11 months of age, osteochondrosis severity correlated negatively with COL2-3/4C(short) and osteocalcin and positively with C-propeptide of type-II procollagen (CPII), a collagen synthesis marker. Radiographic grading of osteochondrosis lesions significantly correlated with macroscopic osteochondrosis severity score at both ages and was strongest when combined with osteocalcin at 5 months and CPII at 11 months. CONCLUSIONS AND CLINICAL RELEVANCE: The ability of serum biomarkers to indicate osteochondrosis severity appears to depend on stage of disease and is strengthened with radiography. In older foals with more permanent lesions, osteochondrosis severity is significantly related to biomarker concentrations of decreased bone formation and increased cartilage synthesis. 相似文献