共查询到20条相似文献，搜索用时 195 毫秒
1.
小鼠输卵管上皮细胞的原代培养及纯化方法研究 总被引：2，自引：0，他引：2
建立小鼠输卵管上皮细胞原代培养及纯化方法.小鼠输卵管上皮细胞原代培养采用组织块培养法和酶消化法.酶消化法于37℃分为4个处理组进行.处理1以2.5 g/L胰酶+0.4 g/L EDTA消化5、10、20 min;处理2以0.5 g/L胰酶+0.08 g/L EDTA消化35、50、75 min;处理3以0.3 g/L的Ⅰ型胶原酶消化60、90、240 min;处理4以2.5 g/L胰酶+0.4 g/L EDTA和0.3 g/L Ⅰ型胶原酶(1∶2)消化60 min,150 min.原代细胞纯化采用差速贴壁和反复差速贴壁法.传代采用胰酶两步消化法进一步纯化输卵管上皮细胞.组织块法原代培养成纤维细胞生长优势明显,传代纯化细胞效果不佳.用酶消化法原代培养时,处理1效果不理想;处理2采用3个消化时间时均取得比较理想的结果;处理3消化240 min时结果较好;处理4消化150 min效果较好.原代细胞纯化,反复差速贴壁得到的上皮细胞较纯,进一步传代纯化细胞取得了理想的结果.小鼠输卵管上皮细胞原代培养采用酶消化法结合反复差速贴壁分离纯化细胞,传代采用两步消化法,可以成功地进行小鼠输卵管上皮细胞的分离纯化培养. 相似文献
2.
3.
4.
初始体重为 2 0 0kg的黑白花奶牛育成牛分别采食东北羊草 +青贮 +混合精料(A组 ) ,未处理稻草颗粒 +青贮 +混合精料 (B组 ) ,处理稻草颗粒 +青贮 +混合料 (C组 ) ,处理稻草颗粒 +混合料和处理稻草颗粒 +甲醛处理混合精料 (E组 ) 5组日粮 ,每组1 0头牛 ,试验期 6 1d ,混合精料和甲醛处理混合精料定量供给 ,每天每头 1 .74kgDM ,玉米青贮也定量供给 ,每天每头 0 .98kgDM ,其余粗饲料自由采食 ,东北羊草 ,未处理稻草颗粒 ,以及C组 ,D组和E组的处理稻草颗粒的采食量分别为 2 .81 ,3 .84,4.1 4,5.1 5和4.98kgDM/(头·d) ,A ,B ,C ,D和E组育成牛平均日增重分别为 797,82 9,90 5,898和91 5g/(头·d) ,其中C ,D和E组的日增重无显著差异 (P >0 .0 5) ,但显著高于A和B组 ;饲料 (kgDM) /增重 (kg)比在各组之间无显著差异 ,用复合化学处理稻草颗粒可完全代替育成牛日粮中的东北羊草和青贮 ,且完全可以保证育成牛的生长发育。但精饲料中的豆饼和玉米经甲醛处理对生长牛的生产性能没有明显影响。 相似文献
5.
全收粪法与三氧化二铬法测定哺乳期犊牛液体饲料养分消化率 总被引：3，自引：0，他引：3
将 16头 10日龄的奶公犊按体重随机分为 4组,每组 4头牛。A、B、C组分别饲喂以植物蛋白为蛋白源的代乳料,D组饲喂牛奶。用全收粪法和外源指示剂三氧化二铬法测定了犊牛对牛奶和代乳料中的各种养分的消化率。试验用全收粪法测得的粗蛋白的消化率(% )分别为 71. 17±7. 48, 66. 19±1. 68, 72. 94±0. 87和 83. 16±1. 73;粗脂肪的消化率分别为 86. 70±1. 94, 85. 13±2. 70, 88. 43±0. 40和 95. 42±0. 49;有机物的消化率分别为 71. 48±6. 80, 65. 45±1. 98,70. 51±0. 60和 77. 31±7. 14。用三氧化二铬法测得的粗蛋白的消化率分别为 82. 50±3. 26, 76. 05±2. 47, 80. 76±1. 37和 85. 62±3. 19;粗脂肪的消化率分别为 91. 72±1. 95, 89. 40±2. 52, 91. 75±0. 92和 96. 09±0. 90;有机物的消化率分别为 82. 63±3. 22, 75. 50±2. 99, 79. 00±1. 93和 81. 48±0. 49。用这两种方法测得的蛋白质、脂肪和有机物的消化率间有中等以上的相关性(0. 56~0. 90)。 相似文献
6.
用从木瓜酶修饰的猪红细胞提取的CD58分子协同NDⅣ疫苗 ,免疫 8日龄雏鸡 (分为试验A组、对照B组 ) ;用从正常猪红细胞提取的CD58分子协同NDⅣ疫苗 ,免疫 18日龄雏鸡 (分为试验C、对照D组 )。以 β_微量法测定ND抗体效价 ,以α_醋酸萘酯酶染色法检测鸡外周血T细胞的变化 ,并分别用F48E9强毒株在免疫后 2 4d攻击A组和B组鸡、39d攻击C组和D组鸡。结果表明 ,免疫前 ,8日龄雏鸡的ND抗体效价为 2 5.8,18日龄雏鸡的ND抗体均为阴性 ;免疫后 ,与对照D组比较 ,试验C组的ND抗体提前 4d达到 2 4.8,并且试验C组ND抗体效价显著高于对照组 (P <0 .0 5或P <0 .0 1) ,保护期延长 ;试验A组雏鸡体内ND抗体变化和对照B组相似 ,但试验A组雏鸡外周血T细胞数量显著高于对照B组 (P <0 .0 1或P <0 .0 5 )。攻毒试验结果显示 ,试验A组鸡死亡率为 33%、对照B组鸡死亡率为 6 3% ;试验C组鸡死亡率为 17% ,对照D组鸡死亡率为 5 3%。本研究结果表明猪红细胞CD58分子具有较好的疫苗佐剂效应。 相似文献
7.
试验旨在探讨稳定可靠的贮精腺上皮细胞分离及原代培养方法,为研究鸡贮精机理提供细胞模型。以鸡输卵管的子宫阴道交接部组织样为材料,采用酶消化法和组织块培养法分离培养母鸡贮精腺上皮细胞,观察母鸡贮精腺上皮细胞的培养情况,比较不同细胞培养方法获得贮精腺上皮细胞的生长情况。结果表明,用胶原酶或胰酶单独消化母鸡子宫阴道交接部组织,经100目过滤后获得的贮精腺上皮细胞24 h后可贴壁,但48～72 h后细胞死亡;用胶原酶Ⅺ(0.01 g/mL)与胰酶(0.25%)先后消化母鸡子宫阴道交接部组织后再经100目过滤获得的贮精腺上皮细胞贴壁性良好,24～48 h细胞出现明显增殖,72 h后细胞增殖速度减慢,开始死亡;用组织块培养法7 d可获得鸡贮精腺上皮原代细胞,该细胞可传2～3代;用组织块培养法获得的细胞进行免疫组化试验,发现细胞表达贮精腺差异表达基因编码的NXPH1蛋白,该蛋白在培养细胞内的表达符合其分泌蛋白特性,表明组织块培养法所获细胞可用于后续研究。综上,用组织块培养法获得的鸡贮精腺上皮细胞可为研究母鸡贮精腺机制提供细胞模型。 相似文献
8.
甘露聚糖酶对大猪生产性能的影响 总被引：2，自引：0，他引：2
为探讨甘露聚糖酶在常规饲养水平下 ,对大猪生产性能及其经济效益的影响 ,试验选用中猪阶段结束后杜×长×大三元杂大猪 72头 ,依据体重、性别比例相近的原则 ,进行分组处理 ;各组基础日粮相同 ,营养水平一致 ,A组饲喂基础日粮 +0 .1 %复合酶A(不含甘露聚糖酶 ) ,B组饲喂基础日粮 +0 .1 %复合酶B(含 1 0 0 0U/g甘露聚糖酶 )、C组饲喂基础日粮 +0 .1 %复合酶C(含2 0 0 0U/g甘露聚糖酶 )。试验期从 2 0 0 2年 9月 30日至 2 0 0 2年 1 0月 30日 ,共 30d。试验结果表明 :试验期日增重A组为 739g、B组为 785g、C组为 835g ,B、C组日增重比对照组分别提高 6 .2 2、1 2 .99;料肉比A、B、C组分别为 3.5 4、3.2 9、3.0 2 ,B、C组比对照组分别降低 7.1、1 4 .7% ;综合经济效益每千克增重饲料成本B组、C组分别为 5 .4 9、5 .0 4元 ,对照组为 5 .91元 ,B、C组经济效益分别提高 7.1、1 4 .7%。 相似文献
9.
试验将自然状态下2～4岁健壮、经产、空怀母羊346只分4组(A组84只、B组68只、C组134只、D组60只),用孕酮阴道栓处理10～14天进行同期发情,其中A组取栓后直接观察发情情况,B组、C组和D组取栓后分别注射FSH25 u、PGF2α0.05～0.1 mg和FSH25 u+PGF2α0.05 mg.处理后2～4天用试情公羊鉴定母羊发情情况,记录发情羊号、出现发情的时间和发情持续时间.发情结束后剖检部分山羊(A组7只,B组9只,C组15只,D组8只),检查卵巢黄体与卵泡发育以及排卵点情况.结果表明A组发情率为48.80%,在4组中最低(P＜0.01);B组和C组分别为75.00%和84.33%,差异不显著(P＞0.05);D组发情率最高,为96.67%,显著高于A组(P＜0.01)与B组(P＜0.05),与C组差异不明显(P＞0.05).A、C组从取栓到出现发情时间分别为50.34 h±18.24 h、45.77 h±17.53 h,显著晚于B组的30.18 h±15.25 h和D组的26.34 h±10.20 h(P＜0.01).4组发情持续的时间分别为34.54 h±12.06 h、36.71 h±10.84 h、37.38 h±12.18 h和36.41 h±10.87 h,没有显著差异(P＞0.05).A、B、C和D组中剖检山羊的85.71%、88.89%、80.00%和100.00%出现排卵点(或新生黄体). 相似文献
10.
绿色添加剂不同添加水平对育成牛增重效果的影响 总被引：1，自引：0，他引：1
试验选择 2 4头平均体重为 35 6kg± 5 4kg的育成牛 ,按单因子随机试验设计分为A、B、C三组 ,每组 8头 ,A、B组为试验组 ,C组为对照组进行试验。A组饲喂精料 +0 5 %酵母培养物 +0 0 5 %寡糖 ;B组饲喂精料 +0 4 %酵母培养物 +0 0 8%寡糖 ;C组饲喂精料。各组精料完全相同且精料中不添加任何抗生素或药物添加剂。结果表明 ,A、B组日增重平均较C组分别提高 16 9%和13 7% ,差异显著 (P <0 0 5 ) ;料重比A、B组平均较C组分别降低 14 7%和 12 1% ,差异显著 (P <0 0 5 ) ;试验期A、B组较C组头均每日增加纯收入 1 12元和 0 83元 ,收益率分别为 1∶3 2和 1∶2 4。 相似文献
11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。 相似文献
12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections
of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal
(GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April
2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs
located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming
areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology
laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum
columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying
degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal
abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy
season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different
nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those
observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes.
But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the
results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia. 相似文献
13.
Tropical Animal Health and Production - 相似文献
14.
Rassnick KM Gieger TL Williams LE Ruslander DM Northrup NC Kristal O Myers NC Moore AS 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2001,15(3):196-199
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks. 相似文献
15.
H Hayashi T Yoshimura J Y Chen 《Comparative immunology, microbiology and infectious diseases》1985,8(2):73-87
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed. 相似文献
16.
Davis MS Willard MD Nelson SL Mandsager RE McKiernan BS Mansell JK Lehenbauer TW 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(3):311-314
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large. 相似文献
17.
Furosemide continuous rate infusion in the horse: evaluation of enhanced efficacy and reduced side effects 总被引：1，自引：0，他引：1
Johansson AM Gardner SY Levine JF Papich MG LaFevers DH Fuquay LR Reagan VH Atkins CE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(6):887-895
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses. 相似文献
18.
B. Cinar Kul N. Bilgen J.A. Lenstra O. Korkmaz Agaoglu B. Akyuz O. Ertugrul 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2015,132(6):449-453
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded. 相似文献
19.
Ling GV Thurmond MC Choi YK Franti CE Ruby AL Johnson DL 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(6):817-823
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors. 相似文献
20.
<正>1.INTRODUCTION Ensuring transportation network security is one Of the most daunting challenges confronting homeland security agencies today.Significant research has been dedicated.To model and analyze the vulnerability of transportation systems,while notably fewer studies propose specific strategies for deploying defensive technologies to safeguard these systems. 相似文献