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1.
Survival of marble goby larvae fed either Rhodovulum sulfidophilum, a phototrophic bacterium cultured from palm oil mill effluent (pPB), or microalgae ( Nannochloropsis sp.) was evaluated at two salinities. Larvae directly fed pPB had survival of 0–29% at 5 g L?1 salinity and 0–19% at 10 g L?1 salinity, whereas larvae directly fed microalgae suffered complete mortality after 20 days of culture at both salinities. However, larvae indirectly fed pPB or microalgae, i.e. via rotifers (Days 1–30) and Artemia nauplii (Days 21–30) cultured solely from pPB or microalgae, showed improved survival of 35–55% or 44–49% at 5 g L?1 salinity respectively. In all experiments, fish larvae reared at 5 g L?1 salinity showed significantly higher (P < 0.01) mean survival than those reared at 10 g L?1 salinity. The survival of larvae fed the bacterial‐based diet was higher compared with microalgal diet used in previous studies. The pPB had higher total polyunsaturated fatty acids and docosahexaenoic acid (DHA) than the microalgae, which had very high eicosapentaenoic acid (EPA). Larvae with very high ratios of DHA/EPA (>11) or/and ARA (arachidonic acid)/EPA (>5), attributable to their given diet, however suffered the highest mortality.  相似文献   

2.
The nitrite toxicity was estimated in juveniles of L. vannamei. The 24, 48, 72 and 96 h LC50 of nitrite‐N on juveniles were 8.1, 7.9, 6.8 and 5.7 mg L?1 at 0.6 g L?1; 14.4, 9.6 8.3 and 7.0 mg L?1 at 1.0 g L?1; 19.4, 15.4, 13.4 and 12.4 mg L?1 at 2.0 g L?1 of salinity respectively. The tolerance of juveniles to nitrite decreased at 96 h of exposure by 18.6% and 54.0%, when salinity declined from 1.0 to 0.6 g L?1 and from 2.0 to 0.6 g L?1 respectively. The safe concentrations at salinities of 0.6, 1.0 and 2.0 g L?1 were 0.28, 0.35 and 0.62 mg L?1 nitrite‐N respectively. The relationship between LC50 (mg L?1), salinity (S) (g L?1) and exposure time (T) (h) was LC50 = 8.4688 + 5.6764S – 0.0762T for salinities from 0.6 to 2.0 g L?1 and for exposure times from 24 to 96 h; the relationship between survival (%) and nitrite‐N concentration (C) for salinity of 0.6–2.0 g L?1, nitrite‐N concentrations of 0–40 mg L?1 and exposure times from 0 to 96 h was as follows: survival (%) = 0.8442 + 0.1909S – 0.0038T – 0.0277C + 0.0008ST + 0.0001CT–0.0029SC, and the tentative equation for predicting the 96‐h LC50 to salinities from 0.6 to 35 g L?1 in L. vannamei juveniles (3.9–4.4 g) was 96‐h LC50 = 0.2127 S2 + 1.558S + 5.9868. For nitrite toxicity, it is shown that a small change in salinity of waters from 2.0 to 0.6 g L?1 is more critical for L. vannamei than when wider differences in salinity occur in brackish and marine waters (15–35 g L?1).  相似文献   

3.
Marbled spinefoot, Siganus rivulatus, is a herbivorous euryhaline teleost widely distributed in the Eastern Mediterranean. It is an economically valuable species and a suitable candidate for warm water aquaculture. Accordingly, understanding the effects of environmental factors on fish metabolism is important to optimize culture conditions. Two experiments were performed to establish standard metabolic rate and study the effect of salinity on metabolism of marbled spinefoot. In the first experiment, a series of flow‐through respirometry experiments was performed at 27°C and 35 g L?1. The standard metabolic rate of marbled spinefoot juveniles was calculated as 0.57 ± 0.02 mg O2 g?1 h?1 (mean ± SE). In the second experiment, fish were maintained at salinities of 25, 30, 35 and 40 g L?1 for 2 weeks. Flow‐through respirometry was performed to measure respiration rates at the various salinities. Respiration rates were similar among fish in salinities of 30, 35 and 40 g L?1 but increased significantly at 25 g L?1. Results suggest that despite the euryhalinity of marbled spinefoot, farmers should maintain salinity within the optimal range of 30–40 g L?1 in order to improve productivity.  相似文献   

4.
In the field, moulting and salinity drop in the water due to excessive rainfall have been mentioned to be risk factors for WSSV outbreaks. Therefore, in this study, the effect of an acute change in environmental salinity and shedding of the old cuticle shell on the susceptibility of Penaeus vannamei to WSSV was evaluated by immersion challenge. For testing the effect of abrupt salinity stress, early premoult shrimp that were acclimated to 35 g L?1 were subjected to salinities of 50 g L?1, 35 g L?1, 20 g L?1, 10 g L?1 and 7 g L?1 or 5 g L?1 and simultaneously exposed to 105.5 SID50 mL?1 of WSSV for 5 h, after which the salinity was brought back to 35 g L?1. Shrimp that were transferred from 35 g L?1 to 50 g L?1, 35 g L?1 and 20 g L?1 did not become infected with WSSV. Shrimp became infected with WSSV after an acute salinity drop from 35 g L?1 to 10 g L?1 and lower. The mortality in shrimp, subjected to a salinity change to 10 g L?1, 7 g L?1 and 5 g L?1, was 6.7%, 46.7% and 53.3%, respectively (P < 0.05). For testing the effect of moulting, shrimp in early premoult, moulting and post‐moult were immersed in sea water containing 105.5 SID50 mL?1 of WSSV. The resulting mortality due to WSSV infection in shrimp inoculated during early premoult (0%), ecdysis (53.3%) and post‐moult (26.72%) demonstrated that a significant difference exists in susceptibility of shrimp during the short moulting process (P < 0.05). The findings of this study indicate that during a drop in environmental salinity lower than 10 g L?1 and ecdysis, shrimp are at risk for a WSSV infection. These findings have important implications for WSSV control measures.  相似文献   

5.
The Asian swamp eel Monopterus albus is normally considered a freshwater species, but can also occasionally be found in brackish water. It is an obligate air‐breather with highly reduced gills, making its osmoregulation physiology interesting because the gills normally represent the primary site of osmoregulation in teleosts. Being a popular fish for human consumption, the swamp eel is cultured extensively in freshwater ponds in the Mekong Delta of Vietnam. However, very little is known about its salinity tolerance, which is becoming topical due to the increasing salt‐water intrusions into tropical deltas. We therefore studied how increased salinity affects survival, growth, blood pressure, heart rate, blood osmolality and plasma ions. All eels survived prolonged exposure to 5 and 10 g L?1, although plasma osmolality increased significantly at 10 g L?1. Further elevation to 15 and 20 g L?1 was associated with significantly elevated mortality, with a corresponding increase in plasma osmolality and ion con‐centrations. Our results show that swamp eels thrive in 0–10 g L?1 with an optimum growth between 0 and 9 g L?1, indicating that utilization of low saline brackish waters for aquaculture is possible.  相似文献   

6.
This study examined the effects of water hardness and salinity on yolk sac larvae and swim‐up fry survival of Nile tilapia, Oreochromis niloticus (Chitralada strain), eggs during artificial incubation. Four experiments were conducted to evaluate the effects of hardness, salinity and the sources of saline incubation water. High water hardness treatments (500–4200 mg L?1 as CaCO3) resulted in higher yolk sac larvae and swim‐up fry survival than low water hardness treatments (50.0 and 132 mg L?1 as CaCO3); although yolk sac larvae and swim‐up fry survival did not differ among the high or low hardness treatments. Salinity of 4.0 g L?1 using seawater, and 4.0 and 8.0 g L?1 using unprocessed common salt resulted in the higher survival rate of yolk sac larvae and swim‐up fry than other salinity treatments. Yolk sac larvae and swim‐up fry survival was found to decrease with the increase in salinity and increase with the increase in water hardness. The present study demonstrated the positive effects of increased water hardness level (>132 mg L?1) on yolk sac larvae and swim‐up fry survival. The study also showed that seawater salinity of 4 g L?1 was the most appropriate salinity level for incubating Nile tilapia eggs.  相似文献   

7.
The experiment was conducted to determine the leucine requirement of juvenile Pacific white shrimp Litopenaeus vannamei (Boone) in low‐salinity water (0.50–1.20 g L?1). Six diets were formulated to contain 410 g kg?1 crude protein with fish meal, peanut meal and precoated crystalline amino acids with different concentration of l ‐leucine (16.72, 19.60, 22.06, 24.79, 27.28 and 30.16 g kg?1 dry diet). Each diet was randomly assigned to triplicate groups of 30 shrimps (0.38 ± 0.002 g), and the feed trial lasted for 8 weeks. The results indicated that the maximum weight gain was observed at 24.95 g kg?1 dietary leucine group, whereas the diets containing higher leucine concentration conversely reduced the growth performance (P < 0.05). Moreover, the highest body protein content and body protein deposition and the lowest haemolymph AST and ALT activities were also found at 24.95 g kg?1 dietary leucine group. With the increase in leucine in diets, a dose‐dependent increase was found in body lipid content and haemolymph urea concentration. The polynomial regression calculated using weight gain, feed efficiency and body protein deposition indicated that the optimal dietary leucine requirement for L. vannamei reared in low‐salinity water was 23.73 g kg?1 leucine of dry diet, correspondingly 57.88 g kg?1 of dietary protein.  相似文献   

8.
Larvae of Metapenaeus monoceros (Fabricius) at protozoea 1 (PZ1) stage were stocked in 2‐L glass flasks to investigate the effects of various salinities (25, 30, 35, 40, 45, 50 and 55 ppt) on growth and survival until the post‐larval (PL) stages. The PZ larvae were not able to tolerate a sudden salinity drop of over 10 ppt. Yet, an abrupt salinity increase of over 10 or even 15 ppt did not cause mortality. The PZ larvae were successfully acclimated to different test salinities at a rate of 4 ppt h?1. The larvae displayed better tolerance to high rather than low salinities. The lowest and highest critical salinities appeared to be 22 and 55 ppt respectively. Taking into account survival, growth and development results, the optimal salinity for the larval culture of M. monoceros inhabiting the Eastern Mediterranean was 40 ppt. At this salinity, the PZ1 larvae were successfully cultured until PL1 stage within 11 days with 68% survival on a feeding regime of Tetraselmis chuii Kylin (Butcher) (20 cells μ L?1), Chaetoceros calcitrans Paulsen (50 cells μ L?1), Isochrysis galbana Parke (30 cells μL?1) and five newly hatched Artemia nauplii mL?1 from M1 onwards at 28 °C.  相似文献   

9.
An 86‐day growth trial was conducted to investigate the effect of dietary oxidized fish oil on the growth and cytopathology of juvenile channel catfish (Ictalurus punctatus). Four diets containing 0 g kg?1 (control: fresh fish oil), 30 g kg?1 (low‐oxidized oil group), 60 g kg?1 (medium‐oxidized oil group) and 90 g kg?1 (high‐oxidized oil group) graded oxidized oil levels with the same dietary lipid level (90 g kg?1 diet) were evaluated. The results show that the specific growth rate decreased with increasing dietary oxidized oil level (< 0.05). All examined liver and kidney tissues in all dose groups exhibited what appeared as dose‐dependent cellular modifications. In addition, lipid droplet accumulation in the hepatocytes of fish in all dose groups was increased, and their localizations were distinctly different between all dose groups. The ultrastructural changes suggest the progression of mitochondrial vacuolation, especially in the renal tubules, in all dose groups. These results reveal a previously underappreciated effect of dietary oxidized fish oil on channel catfish kidneys. Overall, a series of nutriphysiological responses were adversely affected by exposure to dietary oxidized fish oil, and the corresponding interference patterns on the metabolism and transport of nutrients within cells were observed.  相似文献   

10.
The extensively farmed giant freshwater shrimp, Macrobrachium rosenbergii, can survive salinities up to 26 g L?1, but the commercially important grow‐out occurs exclusively in freshwater areas. Recent studies suggest the shrimp equally capable of growing in brackish as fresh water and a better understanding of how this species responds to changing salinity could significantly impact freshwater prawn farming in deltas and coastal areas. Here, the effect of salinity (0 and 15 g L?1) on standard metabolic rate (SMR) and critical oxygen tension (Pcrit) was measured in adult M. rosenbergii using intermittent closed respirometry. SMR was 79.8 ± 3.1 and 72.7 ± 2.9 μmol kg?1 min?1 in fresh and brackish water, respectively, with no significant difference between the two salinities (P = 0.122). During hypoxia M. rosenbergii maintained oxygen uptake down to a Pcrit of 26.3 ± 1.4 mmHg in fresh and 27.2 ± 2.0 mmHg in brackish water (P = 0.682), showing that salinity had no overall effect on oxygen conductance in the animals. These findings are in agreement with recent growth studies and provide further evidence that grow‐out phase could be accomplished in brackish water areas. Thus, the predicted intrusions of brackish water in tropical deltas as a consequence of future global warming may not impact this important production.  相似文献   

11.
The effects of salinity on plasma osmolality, branchial chloride cell density, feed consumption and conversion and growth performance of yellowtail kingfish (Seriola lalandi) were evaluated. Fish (11.6 ± 0.6 g) were kept for 29 days at 14, 18, 22, 26 (experimental) and 30 g L?1 (control) salinity in independent, pilot‐scale recirculation aquaculture systems. No differences in plasma osmolality or chloride cell numbers in gills were observed, pointing to a strong osmoregulatory capacity in the juveniles. Fish at 14, 18 and 22 g L?1 (7.61 ± 0.19, 7.61 ± 0.01 and 7.61 ± 0.13% day?1, respectively) had higher growth rates than fish at 26 and 30 g L?1 (7.10 ± 0.05 and 6.97 ± 0.06% day?1 respectively). The higher growth rate at lower salinity resulted from increased feed intake; feed conversion was not different. An evaluation of the impact of salinity on growth rate of on‐growing stages (till market size) seems warranted to assess whether the profitable effects of low salinity persist in later stages of this important aquaculture species.  相似文献   

12.
Feeding copepods during early larval culture stages of marine fish has proven to be advantageous for growth and survival of marine finfish larvae. However, commercial availability of most copepods is limited; thus, there is an impetus to evaluate promising copepod species to meet the diverse dietary demands of various marine fish. The marine cyclopoid copepod, Oithona colcarva, was isolated out of zooplankton samples taken from waters within Tampa Bay, Florida. Once isolated, trials were conducted to determine the appropriate culture parameters for producing nauplii to feed marine fish larvae. The effects of temperature (22°C, 26°C and 30°C), salinity (15, 20, 25, 30 and 35 g L?1), stocking density (0.5, 1.0, 2.0, 4.0 and 8.0 individuals mL?1) and diet (Nanno 3600 microalgae paste, Colurella adriatica, Rhodomonas lens, Tisochrysis lutea, Chaetoceros gracilis and/or Tetraselmis chuii) on nauplii production during a single life cycle of reproducing individuals were examined. Results of those trials indicated that a culture temperature of 30°C and a salinity of 30 g L?1 were advantageous for maximum nauplii production. Furthermore, a diet containing a 1:1:1 mixture of T. lutea, C. gracilis and T. chuii and a stocking density of at least 8 individuals mL?1 were identified as beneficial. The results of these trials, the potential for large‐scale culture and observations on the performance of marine fish larvae fed Oithona colcarva nauplii are discussed.  相似文献   

13.
This study measures the effect of protein and lipid levels in broodstock diet on the quality of juvenile Cherax quadricarinatus. Diets with different contents of crude protein (180, 250, 310, 370 g kg?1) and lipids (30 and 70 g kg?1) were offered to female crayfish. Juveniles were used to assess initial weight (IW), biochemical composition (BC) and survival to stress tests (ST). After 50 days, growth was estimated. No significant effects of broodstock diet on IW and BC of juveniles were detected. Protein content (PC) significantly influenced growth (P < 0.05), with an optimum level of 250 g kg?1, representing a weight gain of 1.17 g (98.08% body weight). PC significantly influenced survival of juveniles exposed to ST (P < 0.05). Results from fitting dose–response models indicated that maximum survival was obtained with PC above 261, 258 and 312 g kg?1 for hypoxia, salinity, ammonia tests. No significant effect of dietary lipid level was observed on growth and survival to ST, except for greater survival to ammonia ST at lipid content of 70 g kg?1. We recommend feed with PC of 260 g kg?1 to simultaneously enhance growth and quality of juveniles.  相似文献   

14.
To investigate the effect of dietary sodium chloride (NaCl) on meat quality of white shrimp (Litopenaeus vannamei) reared in low‐salinity (2 g L?1) water, shrimp were distributed into four groups (treatments T‐1, T‐2, T‐3 and control) with three replicates. All shrimps were completely randomised stocked into 12 tanks at an initial density of 40 shrimps per tank. Diets for the control, T‐1, T‐2 and T‐3 groups consisted of the basal diet supplemented with 0 g kg?1, 10 g kg?1, 20 g kg?1 and 40 g kg?1 of NaCl respectively. After 50 days, shrimps in T‐3 showed significantly better (< 0.05) moisture, crude protein and ash than those of the control and T‐1. Higher muscle Na content was observed (< 0.05) in T‐3 than that of the control. Significant increases (< 0.05) in contents of inosinic acid, total free amino acid (TFAA) and essential free amino acid (EFAA) were also found in T‐3. Texture assays showed significant differences (< 0.05) in hardness, adhesiveness and springiness between group T‐3 as compared with those of T‐1 and control. It indicated that dietary supplementation of NaCl appeared to be a promising practice to improve meat quality of white shrimp reared in low‐salinity waters.  相似文献   

15.
As marine finfish aquaculture expands, there is an increasing interest in the ability to ship early life stages from breeding centres to hatcheries so that each hatchery does not have to maintain its own broodstock. Here, we conducted 24 h air‐shipping simulations with yolk‐sac larvae of California yellowtail (CYT; Seriola lalandi) and white seabass (WSB; Atractoscion nobilis) to help fill in the informational gaps for shipping marine fish larvae. We examined the effects of a pH buffer on water quality, post‐shipping larval survival and subsequent survival to first feeding at larval densities of 1000, 3000, 6000 and 9000 larvae L?1. The pH buffer, 8.3 Trizma®, was tested at varying concentrations of zero (NT = 0.00 g L?1), low (LT = 0.75 g L?1), medium (MT = 1.5 g L?1) and high (HT = 3.0 g L?1). Trials were conducted using replicate 2 L aquarium bags filled with 500 mL of seawater and held in a water bath at 19–20°C. Results showed an interspecific difference in survival at the highest shipping densities under these experimental conditions. Shipping densities up to 6750 CYT larvae L?1 or 3000 WSB larvae L?1 consistently yielded >90% survival immediately after simulated shipment and >85% survival 48 h after the simulations. Furthermore, at these densities, pH was maintained at ~8.0 when buffered at 1.5 g L?1. The highest tested densities of 9580 CYT larvae L?1 and 9940 WSB larvae L?1, yielded lower survival 69–79% and 0.0–1.3% respectively after 24 h. Final pH in the high density CYT trials were unsatisfactory (below 7.0), regardless of the buffer concentration; however pH in the WSB high density trials improved with increasing buffer concentration. On the basis of the results from these air‐shipping simulations, we recommend CYT and WSB larvae be shipped in seawater with 1.5 g L?1 Trizma® at densities not greater than 6750 larvae L?1 for CYT and 3000 larvae L?1 for WSB. We believe this represents an important step in improving long distance transport protocols for these species and provides useful guidance in air transport of other economically and ecologically important marine species. Additional research is warranted to compare these simulation results with those from actual air shipments, as we did not account for factors that may vary in flight like temperature and pressure variations, and physical agitation.  相似文献   

16.
The effects of dietary calcium chloride (CaCl2) concentration on the growth, survival, moulting and body composition of Astacus leptodactylus were studied. Diets were prepared using supplementation of 0 (control), 30, 60 and 120 g kg?1 calcium chloride commercial trout larvae diet containing 50% protein and 12% lipid. Astacus leptodactylus larvae with an average total length of 22.0 ± 0.05 mm (TL) and weight of 0.53 ± 0.01 g obtained from eighteen ovigerous females collected from Seydisehir Sugla Dam lake in Konya, Turkey, were stocked in 0.2‐m2 aquariums at a rate of 50 crayfish m?2 and reared for 90 days. The experiments included four treatments (diets) with three replicates each. Calcium chloride exerted positive effects on growth, moulting frequency, feed efficiency and survival of narrow‐clawed crayfish. Crayfish fed with 60 g calcium kg?1 calcium chloride‐supplemented diet exhibited the highest weight gain and specific growth rate (SGR) but the lowest survival rate (63.3%) (P < 0.05). There were no significant differences (P > 0.05) in mean moisture (803.5 g kg?1) and protein content (177.0 g kg?1) in crayfish tail meat. However, lipid values were between 3.8 and 11.6 g kg?1 and significantly different among the diets (P < 0.05). Diet with 60 g kg?1 calcium chloride is recommended for the best growth of freshwater crayfish.  相似文献   

17.
More than 250 male and female yellowfin seabream (Acanthopagrus latus) were caught in the creeks near the Mahshar area in the north‐west of Persian Gulf using fishhooks to study the effects of salinity on reproductive indices. The experiments were carried out using three salinity treatments (30 ± 1 g L?1, 35 ± 1 g L?1 and 40 ± 1 g L?1) with three replications. A total of six males and three females were randomly introduced to each tank. The survival rate of the broodstock was estimated at more than 90% at different salinity levels and the maximum rate was observed at 30 g L?1 of the experiment rate. The percentage of buoyant eggs was more than 90% at 40 g L?1 and it was significantly different from other treatments. The average number of eggs per female (312 914 ± 65 085), and the average number of eggs per kilogram of female (649 460 ± 173 574) at 40 g L?1 were more than those in the other treatments but no significant differences were observed (≤ 0.05). The average percentage of fertilized eggs (86.7%) and the average percentage of hatched larvae (67%) at 40 g L?1 treatment was more than those at 30 g L?1 and 35 g L?1 treatments but no significant differences were observed (≤ 0.05). At 40 g L?1 salinity, in all spawning cases, the released eggs were hatched. Overall, the present study has shown that better buoyant eggs of A. latus can be obtained at salinity 40 g L?1. On the other hand, the percentage of fertilized eggs and hatched larvae were not affected by salinity.  相似文献   

18.
The aim of the present work was to obtain the lipid utilization of Octopus vulgaris supplying formulated semi‐moist diets with different contents in cod oil (reduced from water content): 0 g kg?1 (A0, 138 g kg?1 lipids DW; N = 4), 100 g kg?1 (A100, 286 g kg?1 lipids DW; N = 6) and 200 g kg?1 (A200, 388 g kg?1 lipids DW; N = 6). The rest of the ingredients were constant in the three diets: 200 g kg?1 gelatin, 100 g kg?1 egg yolk powder, 150 g kg?1 freeze‐dried Todarodes sagittatus and 50 g kg?1 freeze‐dried Sardinella aurita). Survival was 100% with the three diets. The highest absolute feeding (15.8 ± 1.2 g day?1), growth (9.6 ± 1.4 g day?1; 0.91% BW day?1) and feed efficiency rates (60.3%) were obtained with diet A0. This diet also showed greater retention of lipid and protein than A100 and A200. Protein digestibility was above 95% in all of the diets. Only diet A0 led to a high lipid digestibility coefficient (81.25%), which fell drastically to 12.3% in A200. It was notable the high polar lipid digestibility rates (83–89%) respect to neutral lipids (2–87%) in all diets. The best results were obtained with lipid feeding rates of around 1 g day?1 and a suitable lipid content on 130–140 g kg?1 DW in formulated diets for O. vulgaris.  相似文献   

19.
Nutritional efficacy of fairy shrimp (Streptocephalus sirindhornae) nauplii, as a live food, was studied for growth performance and survival rate of giant freshwater prawn (Macrobrachium rosenbergii) postlarvae. A feeding experiment was designed with four different feeds: dry commercial feed, fairy shrimp nauplii, Artemia sp. nauplii and adult Moina macrocopa. Results from the nutritional composition revealed that fairy shrimp nauplii had protein and lipid contents of 54.58 ± 2.8 g kg?1 and 255 ± 2.8 g kg?1, respectively. The highest value for an individual amino acid in fairy shrimp was lysine (140.7 ± 1.6 g kg?1). The essential amino acids content in the whole body of the larval prawns was in the range of 66.7–67.5 g kg?1. Fairy shrimp nauplii had the highest essential amino acid ratio (A/E) of lysine, similarly, in musculature of prawn larvae. Weight gain and specific growth rate of the postlarvae fed with fairy shrimp nauplii were significantly higher than those fed with Artemia nauplii, adult Moina and dry commercial feed. The presented results suggest that S. sirindhornae nauplii can be used as a nutritionally adequate food for freshwater prawn M. rosenbergii postlarvae.  相似文献   

20.
Sexually mature kutum, Rutilus frisii kutum, captured from its natural habits, the Caspian Sea and the Khoushkrood River, reared at 0.5 g L?1 and 8–13 g L?1 for approximately 1 year in experimental condition, for assessing the effect of salinity on reproduction. Plasma concentrations of sex steroid hormones (17β‐estradiol, testosterone and 17α‐hydroxyprogestrone) were measured in the three stages of gametogenesis. Female kutum held at <0.5 g L?1 or 8–13 g L?1 had no ovulated oocytes in their ovaries. In contrast, males held in captivity were spermiated, similar to their wild counterparts. The average sperm volume of males held at <0.5 g L?1 (2.36 ± 0.46 mL) was lower than males held at 8–13 g L?1 (3.65 ± 0.73 mL) at the end of the experimental period. The highest concentration of testosterone was observed in mid‐gametogenesis in wild fish that was significantly higher than the concentration seen in fish held in either <0.5 g L?1 or 8–13 g L?1. Female kutum showed suppressed steroid hormones in captivity, resulting in failure in the gonad development. However, male kutum adapt well to captivity and showed synchrony in steroid hormone variations with the wild fish, resulting in the testicular development. Results of this study also indicate that salinity plays a minor, but vital, role in reproduction of kutum, a factor that needs to be considered for keeping broodstock of brackish water fish species like kutum.  相似文献   

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